Experiment: carbon source Sucrose 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5113 and Pf1N1B4_5114 overlap by 4 nucleotides Pf1N1B4_5114 and Pf1N1B4_5115 are separated by 27 nucleotides Pf1N1B4_5115 and Pf1N1B4_5116 are separated by 29 nucleotides
Pf1N1B4_5113 - sucrose ABC transporter, permease component 1 (from data) , at 5,672,987 to 5,673,874
_5113
Pf1N1B4_5114 - sucrose ABC transporter, permease component 2 (from data) , at 5,673,871 to 5,674,713
_5114
Pf1N1B4_5115 - sucrose ABC transporter, ATPase component (from data) , at 5,674,741 to 5,675,886
_5115
Pf1N1B4_5116 - Sucrose alpha-glucosidase (EC 3.2.1.48) (from data) , at 5,675,916 to 5,677,385
_5116
Position (kb)
5674
5675
5676 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 5674.045 kb on + strand, within Pf1N1B4_5114 at 5674.476 kb on - strand, within Pf1N1B4_5114 at 5674.792 kb on - strand at 5674.999 kb on + strand, within Pf1N1B4_5115 at 5675.305 kb on + strand, within Pf1N1B4_5115 at 5675.306 kb on - strand, within Pf1N1B4_5115 at 5675.404 kb on + strand, within Pf1N1B4_5115 at 5675.404 kb on + strand at 5675.405 kb on - strand, within Pf1N1B4_5115 at 5675.654 kb on - strand, within Pf1N1B4_5115 at 5675.671 kb on + strand, within Pf1N1B4_5115 at 5675.671 kb on + strand, within Pf1N1B4_5115 at 5675.671 kb on + strand at 5675.672 kb on - strand, within Pf1N1B4_5115 at 5675.883 kb on - strand at 5676.046 kb on - strand at 5676.073 kb on - strand, within Pf1N1B4_5116 at 5676.073 kb on - strand at 5676.111 kb on + strand, within Pf1N1B4_5116 at 5676.249 kb on + strand, within Pf1N1B4_5116 at 5676.250 kb on - strand, within Pf1N1B4_5116 at 5676.409 kb on + strand, within Pf1N1B4_5116 at 5676.433 kb on - strand, within Pf1N1B4_5116 at 5676.501 kb on - strand, within Pf1N1B4_5116 at 5676.501 kb on - strand, within Pf1N1B4_5116 at 5676.519 kb on + strand, within Pf1N1B4_5116 at 5676.549 kb on + strand at 5676.550 kb on - strand, within Pf1N1B4_5116 at 5676.551 kb on + strand, within Pf1N1B4_5116 at 5676.551 kb on + strand, within Pf1N1B4_5116 at 5676.551 kb on + strand, within Pf1N1B4_5116 at 5676.551 kb on + strand, within Pf1N1B4_5116 at 5676.551 kb on + strand, within Pf1N1B4_5116 at 5676.551 kb on + strand, within Pf1N1B4_5116 at 5676.551 kb on + strand, within Pf1N1B4_5116 at 5676.552 kb on - strand, within Pf1N1B4_5116 at 5676.595 kb on + strand, within Pf1N1B4_5116 at 5676.596 kb on - strand, within Pf1N1B4_5116 at 5676.596 kb on - strand at 5676.596 kb on - strand, within Pf1N1B4_5116 at 5676.596 kb on - strand, within Pf1N1B4_5116 at 5676.603 kb on + strand, within Pf1N1B4_5116 at 5676.604 kb on - strand, within Pf1N1B4_5116 at 5676.652 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 1mM remove 5,674,045 + Pf1N1B4_5114 0.21 -1.6 5,674,476 - Pf1N1B4_5114 0.72 -2.1 5,674,792 - -2.0 5,674,999 + Pf1N1B4_5115 0.23 -1.3 5,675,305 + Pf1N1B4_5115 0.49 -1.5 5,675,306 - Pf1N1B4_5115 0.49 -2.1 5,675,404 + Pf1N1B4_5115 0.58 -2.3 5,675,404 + -1.2 5,675,405 - Pf1N1B4_5115 0.58 -3.3 5,675,654 - Pf1N1B4_5115 0.80 -4.1 5,675,671 + Pf1N1B4_5115 0.81 -1.8 5,675,671 + Pf1N1B4_5115 0.81 -3.9 5,675,671 + -2.1 5,675,672 - Pf1N1B4_5115 0.81 -1.4 5,675,883 - -3.8 5,676,046 - -1.2 5,676,073 - Pf1N1B4_5116 0.11 -1.9 5,676,073 - -0.7 5,676,111 + Pf1N1B4_5116 0.13 -3.2 5,676,249 + Pf1N1B4_5116 0.23 -2.4 5,676,250 - Pf1N1B4_5116 0.23 +1.2 5,676,409 + Pf1N1B4_5116 0.34 -3.3 5,676,433 - Pf1N1B4_5116 0.35 -3.1 5,676,501 - Pf1N1B4_5116 0.40 -1.7 5,676,501 - Pf1N1B4_5116 0.40 -2.0 5,676,519 + Pf1N1B4_5116 0.41 -4.8 5,676,549 + -1.1 5,676,550 - Pf1N1B4_5116 0.43 -2.8 5,676,551 + Pf1N1B4_5116 0.43 -2.8 5,676,551 + Pf1N1B4_5116 0.43 -0.5 5,676,551 + Pf1N1B4_5116 0.43 -1.7 5,676,551 + Pf1N1B4_5116 0.43 -1.2 5,676,551 + Pf1N1B4_5116 0.43 -2.4 5,676,551 + Pf1N1B4_5116 0.43 -3.5 5,676,551 + Pf1N1B4_5116 0.43 -2.0 5,676,552 - Pf1N1B4_5116 0.43 -3.2 5,676,595 + Pf1N1B4_5116 0.46 -2.5 5,676,596 - Pf1N1B4_5116 0.46 +1.2 5,676,596 - +1.1 5,676,596 - Pf1N1B4_5116 0.46 -0.3 5,676,596 - Pf1N1B4_5116 0.46 -3.6 5,676,603 + Pf1N1B4_5116 0.47 -1.4 5,676,604 - Pf1N1B4_5116 0.47 -3.1 5,676,652 - +1.3
Or see this region's nucleotide sequence