Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4684

Experiment: carbon source Sucrose 1mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4682 and Pf1N1B4_4683 are separated by 128 nucleotidesPf1N1B4_4683 and Pf1N1B4_4684 are separated by 21 nucleotidesPf1N1B4_4684 and Pf1N1B4_4685 overlap by 4 nucleotides Pf1N1B4_4682 - Glycogen debranching enzyme (EC 3.2.1.-), at 5,169,010 to 5,171,169 _4682 Pf1N1B4_4683 - FIG00953356: hypothetical protein, at 5,171,298 to 5,171,645 _4683 Pf1N1B4_4684 - Malto-oligosyltrehalose synthase (EC 5.4.99.15), at 5,171,667 to 5,174,465 _4684 Pf1N1B4_4685 - 4-alpha-glucanotransferase (amylomaltase) (EC 2.4.1.25), at 5,174,462 to 5,176,540 _4685 Position (kb) 5171 5172 5173 5174 5175Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 5170.898 kb on + strand, within Pf1N1B4_4682at 5170.913 kb on + strand, within Pf1N1B4_4682at 5170.943 kb on + strand, within Pf1N1B4_4682at 5170.967 kb on + strandat 5171.169 kb on + strandat 5171.169 kb on + strandat 5171.752 kb on - strandat 5171.752 kb on - strandat 5171.887 kb on - strandat 5171.889 kb on + strandat 5171.889 kb on + strandat 5171.974 kb on + strand, within Pf1N1B4_4684at 5171.975 kb on - strandat 5171.975 kb on - strand, within Pf1N1B4_4684at 5171.975 kb on - strand, within Pf1N1B4_4684at 5172.093 kb on + strand, within Pf1N1B4_4684at 5172.093 kb on + strand, within Pf1N1B4_4684at 5172.093 kb on + strand, within Pf1N1B4_4684at 5172.093 kb on + strand, within Pf1N1B4_4684at 5172.122 kb on - strand, within Pf1N1B4_4684at 5172.758 kb on - strand, within Pf1N1B4_4684at 5173.045 kb on + strand, within Pf1N1B4_4684at 5173.045 kb on + strandat 5173.045 kb on + strand, within Pf1N1B4_4684at 5173.045 kb on + strand, within Pf1N1B4_4684at 5173.045 kb on + strand, within Pf1N1B4_4684at 5173.046 kb on - strand, within Pf1N1B4_4684at 5173.046 kb on - strand, within Pf1N1B4_4684at 5173.046 kb on - strand, within Pf1N1B4_4684at 5173.068 kb on + strand, within Pf1N1B4_4684at 5173.720 kb on + strand, within Pf1N1B4_4684at 5173.720 kb on + strandat 5173.720 kb on + strand, within Pf1N1B4_4684at 5173.720 kb on + strand, within Pf1N1B4_4684at 5173.721 kb on - strand, within Pf1N1B4_4684at 5173.721 kb on - strand, within Pf1N1B4_4684at 5173.835 kb on + strand, within Pf1N1B4_4684at 5174.342 kb on - strandat 5174.371 kb on + strandat 5174.947 kb on + strand, within Pf1N1B4_4685at 5175.111 kb on + strand, within Pf1N1B4_4685at 5175.117 kb on + strand, within Pf1N1B4_4685

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source Sucrose 1mM
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5,170,898 + Pf1N1B4_4682 0.87 -2.9
5,170,913 + Pf1N1B4_4682 0.88 -1.8
5,170,943 + Pf1N1B4_4682 0.89 +1.4
5,170,967 + +1.8
5,171,169 + +0.4
5,171,169 + -1.3
5,171,752 - -0.3
5,171,752 - -0.5
5,171,887 - +0.5
5,171,889 + +1.0
5,171,889 + +1.9
5,171,974 + Pf1N1B4_4684 0.11 -2.8
5,171,975 - -2.3
5,171,975 - Pf1N1B4_4684 0.11 -0.3
5,171,975 - Pf1N1B4_4684 0.11 -3.4
5,172,093 + Pf1N1B4_4684 0.15 +0.2
5,172,093 + Pf1N1B4_4684 0.15 +2.9
5,172,093 + Pf1N1B4_4684 0.15 +0.2
5,172,093 + Pf1N1B4_4684 0.15 +1.4
5,172,122 - Pf1N1B4_4684 0.16 +2.2
5,172,758 - Pf1N1B4_4684 0.39 -2.2
5,173,045 + Pf1N1B4_4684 0.49 -1.6
5,173,045 + +0.8
5,173,045 + Pf1N1B4_4684 0.49 -1.7
5,173,045 + Pf1N1B4_4684 0.49 +1.0
5,173,045 + Pf1N1B4_4684 0.49 -3.4
5,173,046 - Pf1N1B4_4684 0.49 -1.6
5,173,046 - Pf1N1B4_4684 0.49 -2.1
5,173,046 - Pf1N1B4_4684 0.49 -2.2
5,173,068 + Pf1N1B4_4684 0.50 +1.0
5,173,720 + Pf1N1B4_4684 0.73 +0.3
5,173,720 + -1.0
5,173,720 + Pf1N1B4_4684 0.73 +3.3
5,173,720 + Pf1N1B4_4684 0.73 +1.5
5,173,721 - Pf1N1B4_4684 0.73 -0.6
5,173,721 - Pf1N1B4_4684 0.73 +0.0
5,173,835 + Pf1N1B4_4684 0.77 +1.0
5,174,342 - +0.4
5,174,371 + +1.9
5,174,947 + Pf1N1B4_4685 0.23 -0.3
5,175,111 + Pf1N1B4_4685 0.31 +0.2
5,175,117 + Pf1N1B4_4685 0.32 -1.3

Or see this region's nucleotide sequence