Experiment: carbon source Sucrose 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4682 and Pf1N1B4_4683 are separated by 128 nucleotides Pf1N1B4_4683 and Pf1N1B4_4684 are separated by 21 nucleotides Pf1N1B4_4684 and Pf1N1B4_4685 overlap by 4 nucleotides
Pf1N1B4_4682 - Glycogen debranching enzyme (EC 3.2.1.-), at 5,169,010 to 5,171,169
_4682
Pf1N1B4_4683 - FIG00953356: hypothetical protein, at 5,171,298 to 5,171,645
_4683
Pf1N1B4_4684 - Malto-oligosyltrehalose synthase (EC 5.4.99.15), at 5,171,667 to 5,174,465
_4684
Pf1N1B4_4685 - 4-alpha-glucanotransferase (amylomaltase) (EC 2.4.1.25), at 5,174,462 to 5,176,540
_4685
Position (kb)
5171
5172
5173
5174
5175 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5170.898 kb on + strand, within Pf1N1B4_4682 at 5170.913 kb on + strand, within Pf1N1B4_4682 at 5170.943 kb on + strand, within Pf1N1B4_4682 at 5170.967 kb on + strand at 5171.169 kb on + strand at 5171.169 kb on + strand at 5171.752 kb on - strand at 5171.752 kb on - strand at 5171.887 kb on - strand at 5171.889 kb on + strand at 5171.889 kb on + strand at 5171.974 kb on + strand, within Pf1N1B4_4684 at 5171.975 kb on - strand at 5171.975 kb on - strand, within Pf1N1B4_4684 at 5171.975 kb on - strand, within Pf1N1B4_4684 at 5172.093 kb on + strand, within Pf1N1B4_4684 at 5172.093 kb on + strand, within Pf1N1B4_4684 at 5172.093 kb on + strand, within Pf1N1B4_4684 at 5172.093 kb on + strand, within Pf1N1B4_4684 at 5172.122 kb on - strand, within Pf1N1B4_4684 at 5172.758 kb on - strand, within Pf1N1B4_4684 at 5173.045 kb on + strand, within Pf1N1B4_4684 at 5173.045 kb on + strand at 5173.045 kb on + strand, within Pf1N1B4_4684 at 5173.045 kb on + strand, within Pf1N1B4_4684 at 5173.045 kb on + strand, within Pf1N1B4_4684 at 5173.046 kb on - strand, within Pf1N1B4_4684 at 5173.046 kb on - strand, within Pf1N1B4_4684 at 5173.046 kb on - strand, within Pf1N1B4_4684 at 5173.068 kb on + strand, within Pf1N1B4_4684 at 5173.720 kb on + strand, within Pf1N1B4_4684 at 5173.720 kb on + strand at 5173.720 kb on + strand, within Pf1N1B4_4684 at 5173.720 kb on + strand, within Pf1N1B4_4684 at 5173.721 kb on - strand, within Pf1N1B4_4684 at 5173.721 kb on - strand, within Pf1N1B4_4684 at 5173.835 kb on + strand, within Pf1N1B4_4684 at 5174.342 kb on - strand at 5174.371 kb on + strand at 5174.947 kb on + strand, within Pf1N1B4_4685 at 5175.111 kb on + strand, within Pf1N1B4_4685 at 5175.117 kb on + strand, within Pf1N1B4_4685
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 1mM remove 5,170,898 + Pf1N1B4_4682 0.87 -2.9 5,170,913 + Pf1N1B4_4682 0.88 -1.8 5,170,943 + Pf1N1B4_4682 0.89 +1.4 5,170,967 + +1.8 5,171,169 + +0.4 5,171,169 + -1.3 5,171,752 - -0.3 5,171,752 - -0.5 5,171,887 - +0.5 5,171,889 + +1.0 5,171,889 + +1.9 5,171,974 + Pf1N1B4_4684 0.11 -2.8 5,171,975 - -2.3 5,171,975 - Pf1N1B4_4684 0.11 -0.3 5,171,975 - Pf1N1B4_4684 0.11 -3.4 5,172,093 + Pf1N1B4_4684 0.15 +0.2 5,172,093 + Pf1N1B4_4684 0.15 +2.9 5,172,093 + Pf1N1B4_4684 0.15 +0.2 5,172,093 + Pf1N1B4_4684 0.15 +1.4 5,172,122 - Pf1N1B4_4684 0.16 +2.2 5,172,758 - Pf1N1B4_4684 0.39 -2.2 5,173,045 + Pf1N1B4_4684 0.49 -1.6 5,173,045 + +0.8 5,173,045 + Pf1N1B4_4684 0.49 -1.7 5,173,045 + Pf1N1B4_4684 0.49 +1.0 5,173,045 + Pf1N1B4_4684 0.49 -3.4 5,173,046 - Pf1N1B4_4684 0.49 -1.6 5,173,046 - Pf1N1B4_4684 0.49 -2.1 5,173,046 - Pf1N1B4_4684 0.49 -2.2 5,173,068 + Pf1N1B4_4684 0.50 +1.0 5,173,720 + Pf1N1B4_4684 0.73 +0.3 5,173,720 + -1.0 5,173,720 + Pf1N1B4_4684 0.73 +3.3 5,173,720 + Pf1N1B4_4684 0.73 +1.5 5,173,721 - Pf1N1B4_4684 0.73 -0.6 5,173,721 - Pf1N1B4_4684 0.73 +0.0 5,173,835 + Pf1N1B4_4684 0.77 +1.0 5,174,342 - +0.4 5,174,371 + +1.9 5,174,947 + Pf1N1B4_4685 0.23 -0.3 5,175,111 + Pf1N1B4_4685 0.31 +0.2 5,175,117 + Pf1N1B4_4685 0.32 -1.3
Or see this region's nucleotide sequence