Experiment: carbon source Sucrose 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4675 and Pf1N1B4_4676 are separated by 414 nucleotides Pf1N1B4_4676 and Pf1N1B4_4677 are separated by 152 nucleotides
Pf1N1B4_4675 - Mg(2+) transport ATPase protein C, at 5,156,737 to 5,157,210
_4675
Pf1N1B4_4676 - Alpha-amylase (EC 3.2.1.1), at 5,157,625 to 5,159,622
_4676
Pf1N1B4_4677 - Trehalose synthase (EC 5.4.99.16), at 5,159,775 to 5,163,116
_4677
Position (kb)
5157
5158
5159
5160 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 5156.669 kb on + strand at 5156.669 kb on + strand at 5156.669 kb on + strand at 5156.669 kb on + strand at 5156.669 kb on + strand at 5156.669 kb on + strand at 5156.670 kb on - strand at 5157.135 kb on + strand, within Pf1N1B4_4675 at 5157.225 kb on - strand at 5157.463 kb on - strand at 5157.538 kb on + strand at 5157.538 kb on + strand at 5157.655 kb on + strand at 5157.666 kb on + strand at 5157.666 kb on + strand at 5157.931 kb on + strand, within Pf1N1B4_4676 at 5157.931 kb on + strand, within Pf1N1B4_4676 at 5157.932 kb on - strand at 5158.268 kb on - strand, within Pf1N1B4_4676 at 5158.375 kb on + strand, within Pf1N1B4_4676 at 5158.376 kb on - strand, within Pf1N1B4_4676 at 5158.376 kb on - strand, within Pf1N1B4_4676 at 5158.720 kb on + strand at 5158.721 kb on - strand at 5158.957 kb on + strand, within Pf1N1B4_4676 at 5158.958 kb on - strand, within Pf1N1B4_4676 at 5158.963 kb on + strand, within Pf1N1B4_4676 at 5158.963 kb on + strand, within Pf1N1B4_4676 at 5158.964 kb on - strand at 5158.964 kb on - strand, within Pf1N1B4_4676 at 5158.964 kb on - strand, within Pf1N1B4_4676 at 5158.964 kb on - strand, within Pf1N1B4_4676 at 5159.002 kb on + strand at 5159.003 kb on - strand, within Pf1N1B4_4676 at 5159.065 kb on - strand, within Pf1N1B4_4676 at 5159.164 kb on + strand, within Pf1N1B4_4676 at 5159.164 kb on + strand, within Pf1N1B4_4676 at 5159.215 kb on + strand, within Pf1N1B4_4676 at 5159.377 kb on + strand, within Pf1N1B4_4676 at 5159.377 kb on + strand, within Pf1N1B4_4676 at 5159.378 kb on - strand, within Pf1N1B4_4676 at 5159.557 kb on + strand at 5159.557 kb on + strand at 5159.557 kb on + strand at 5159.558 kb on - strand at 5159.610 kb on + strand at 5159.610 kb on + strand at 5159.613 kb on + strand at 5159.613 kb on + strand at 5159.613 kb on + strand at 5159.614 kb on - strand at 5159.754 kb on + strand at 5159.755 kb on - strand at 5159.757 kb on + strand at 5159.757 kb on + strand at 5159.764 kb on - strand at 5159.771 kb on - strand at 5159.841 kb on + strand at 5159.967 kb on + strand at 5159.994 kb on + strand at 5159.995 kb on - strand at 5160.020 kb on - strand at 5160.020 kb on - strand at 5160.187 kb on - strand, within Pf1N1B4_4677 at 5160.187 kb on - strand at 5160.187 kb on - strand, within Pf1N1B4_4677 at 5160.187 kb on - strand, within Pf1N1B4_4677 at 5160.214 kb on - strand, within Pf1N1B4_4677 at 5160.214 kb on - strand, within Pf1N1B4_4677 at 5160.285 kb on + strand at 5160.285 kb on + strand, within Pf1N1B4_4677 at 5160.286 kb on - strand, within Pf1N1B4_4677 at 5160.304 kb on - strand, within Pf1N1B4_4677 at 5160.304 kb on - strand, within Pf1N1B4_4677 at 5160.304 kb on - strand, within Pf1N1B4_4677 at 5160.568 kb on - strand, within Pf1N1B4_4677
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 1mM remove 5,156,669 + -1.8 5,156,669 + +0.5 5,156,669 + +0.0 5,156,669 + -1.7 5,156,669 + -1.2 5,156,669 + -1.9 5,156,670 - -1.9 5,157,135 + Pf1N1B4_4675 0.84 +1.6 5,157,225 - +1.0 5,157,463 - -1.2 5,157,538 + -1.5 5,157,538 + -2.6 5,157,655 + -0.8 5,157,666 + +5.0 5,157,666 + +0.3 5,157,931 + Pf1N1B4_4676 0.15 -2.5 5,157,931 + Pf1N1B4_4676 0.15 -2.5 5,157,932 - -1.0 5,158,268 - Pf1N1B4_4676 0.32 -1.6 5,158,375 + Pf1N1B4_4676 0.38 +1.3 5,158,376 - Pf1N1B4_4676 0.38 -1.5 5,158,376 - Pf1N1B4_4676 0.38 -3.2 5,158,720 + +1.0 5,158,721 - -2.1 5,158,957 + Pf1N1B4_4676 0.67 +0.0 5,158,958 - Pf1N1B4_4676 0.67 +1.1 5,158,963 + Pf1N1B4_4676 0.67 +0.4 5,158,963 + Pf1N1B4_4676 0.67 +0.4 5,158,964 - -1.2 5,158,964 - Pf1N1B4_4676 0.67 +2.5 5,158,964 - Pf1N1B4_4676 0.67 -1.5 5,158,964 - Pf1N1B4_4676 0.67 -0.2 5,159,002 + -0.6 5,159,003 - Pf1N1B4_4676 0.69 -0.8 5,159,065 - Pf1N1B4_4676 0.72 +0.4 5,159,164 + Pf1N1B4_4676 0.77 +2.3 5,159,164 + Pf1N1B4_4676 0.77 -2.5 5,159,215 + Pf1N1B4_4676 0.80 -1.5 5,159,377 + Pf1N1B4_4676 0.88 -1.3 5,159,377 + Pf1N1B4_4676 0.88 -2.5 5,159,378 - Pf1N1B4_4676 0.88 -2.3 5,159,557 + +2.1 5,159,557 + -0.3 5,159,557 + -1.8 5,159,558 - +2.9 5,159,610 + +0.5 5,159,610 + -0.5 5,159,613 + -2.2 5,159,613 + -2.6 5,159,613 + +0.5 5,159,614 - -0.7 5,159,754 + +0.8 5,159,755 - -1.6 5,159,757 + -1.6 5,159,757 + -0.8 5,159,764 - -1.3 5,159,771 - -1.8 5,159,841 + +2.0 5,159,967 + -0.9 5,159,994 + -2.1 5,159,995 - -1.7 5,160,020 - -1.7 5,160,020 - -2.1 5,160,187 - Pf1N1B4_4677 0.12 -2.2 5,160,187 - +0.0 5,160,187 - Pf1N1B4_4677 0.12 -1.1 5,160,187 - Pf1N1B4_4677 0.12 +1.0 5,160,214 - Pf1N1B4_4677 0.13 -0.9 5,160,214 - Pf1N1B4_4677 0.13 +2.4 5,160,285 + +1.7 5,160,285 + Pf1N1B4_4677 0.15 +1.1 5,160,286 - Pf1N1B4_4677 0.15 +0.6 5,160,304 - Pf1N1B4_4677 0.16 +0.9 5,160,304 - Pf1N1B4_4677 0.16 -1.5 5,160,304 - Pf1N1B4_4677 0.16 -1.3 5,160,568 - Pf1N1B4_4677 0.24 +0.2
Or see this region's nucleotide sequence