Experiment: carbon source Sucrose 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3648 and Pf1N1B4_3649 are separated by 68 nucleotides Pf1N1B4_3649 and Pf1N1B4_3650 are separated by 273 nucleotides Pf1N1B4_3650 and Pf1N1B4_3651 overlap by 1 nucleotides Pf1N1B4_3651 and Pf1N1B4_3652 are separated by 289 nucleotides
Pf1N1B4_3648 - hypothetical protein, at 4,053,995 to 4,054,498
_3648
Pf1N1B4_3649 - Excisionase domain protein, at 4,054,567 to 4,054,998
_3649
Pf1N1B4_3650 - hypothetical protein, at 4,055,272 to 4,055,487
_3650
Pf1N1B4_3651 - Antitoxin 1, at 4,055,487 to 4,055,891
_3651
Pf1N1B4_3652 - Mobile element protein, at 4,056,181 to 4,057,209
_3652
Position (kb)
4055
4056 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4054.542 kb on + strand at 4054.542 kb on + strand at 4054.543 kb on - strand at 4054.544 kb on + strand at 4054.544 kb on + strand at 4054.544 kb on + strand at 4054.544 kb on + strand at 4054.544 kb on + strand at 4054.544 kb on + strand at 4054.545 kb on - strand at 4054.545 kb on - strand at 4054.545 kb on - strand at 4054.545 kb on - strand at 4054.545 kb on - strand at 4054.545 kb on - strand at 4054.545 kb on - strand at 4054.545 kb on - strand at 4055.114 kb on + strand at 4055.114 kb on + strand at 4055.115 kb on - strand at 4055.183 kb on + strand at 4055.375 kb on - strand, within Pf1N1B4_3650 at 4055.748 kb on + strand, within Pf1N1B4_3651 at 4055.749 kb on - strand, within Pf1N1B4_3651 at 4055.814 kb on + strand, within Pf1N1B4_3651 at 4055.896 kb on + strand at 4055.930 kb on - strand at 4055.930 kb on - strand at 4055.936 kb on - strand at 4055.936 kb on - strand at 4055.936 kb on - strand at 4055.936 kb on - strand at 4055.936 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 1mM remove 4,054,542 + +0.9 4,054,542 + +0.7 4,054,543 - -0.0 4,054,544 + -1.6 4,054,544 + -1.2 4,054,544 + +1.3 4,054,544 + +0.4 4,054,544 + -1.6 4,054,544 + +0.0 4,054,545 - -0.2 4,054,545 - +0.6 4,054,545 - +2.4 4,054,545 - -0.3 4,054,545 - +0.4 4,054,545 - -1.3 4,054,545 - +0.5 4,054,545 - -0.4 4,055,114 + +0.2 4,055,114 + -1.9 4,055,115 - +0.4 4,055,183 + -1.5 4,055,375 - Pf1N1B4_3650 0.48 +1.6 4,055,748 + Pf1N1B4_3651 0.64 -1.2 4,055,749 - Pf1N1B4_3651 0.65 -2.6 4,055,814 + Pf1N1B4_3651 0.81 -1.8 4,055,896 + +2.1 4,055,930 - -0.0 4,055,930 - -1.0 4,055,936 - +1.5 4,055,936 - -2.1 4,055,936 - +0.1 4,055,936 - -2.5 4,055,936 - -1.8
Or see this region's nucleotide sequence