Experiment: carbon source Sucrose 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3271 and Pf1N1B4_3272 are separated by 19 nucleotides Pf1N1B4_3272 and Pf1N1B4_3273 are separated by 79 nucleotides Pf1N1B4_3273 and Pf1N1B4_3274 are separated by 307 nucleotides Pf1N1B4_3274 and Pf1N1B4_3275 are separated by 64 nucleotides
Pf1N1B4_3271 - Bicyclomycin resistance protein, at 3,661,251 to 3,662,417
_3271
Pf1N1B4_3272 - hypothetical protein, at 3,662,437 to 3,662,553
_3272
Pf1N1B4_3273 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4), at 3,662,633 to 3,662,893
_3273
Pf1N1B4_3274 - Fosfomycin resistance protein FosA, at 3,663,201 to 3,663,617
_3274
Pf1N1B4_3275 - L-lysine permease, at 3,663,682 to 3,664,320
_3275
Position (kb)
3662
3663 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3661.660 kb on + strand, within Pf1N1B4_3271 at 3661.661 kb on - strand, within Pf1N1B4_3271 at 3661.661 kb on - strand, within Pf1N1B4_3271 at 3661.828 kb on + strand, within Pf1N1B4_3271 at 3661.931 kb on - strand, within Pf1N1B4_3271 at 3661.931 kb on - strand, within Pf1N1B4_3271 at 3661.931 kb on - strand, within Pf1N1B4_3271 at 3661.931 kb on - strand, within Pf1N1B4_3271 at 3661.931 kb on - strand, within Pf1N1B4_3271 at 3661.979 kb on - strand, within Pf1N1B4_3271 at 3662.179 kb on + strand, within Pf1N1B4_3271 at 3662.179 kb on + strand, within Pf1N1B4_3271 at 3662.179 kb on + strand, within Pf1N1B4_3271 at 3662.180 kb on - strand, within Pf1N1B4_3271 at 3662.180 kb on - strand, within Pf1N1B4_3271 at 3662.180 kb on - strand at 3662.180 kb on - strand, within Pf1N1B4_3271 at 3662.180 kb on - strand, within Pf1N1B4_3271 at 3662.184 kb on - strand, within Pf1N1B4_3271 at 3662.272 kb on + strand, within Pf1N1B4_3271 at 3662.272 kb on + strand, within Pf1N1B4_3271 at 3662.272 kb on + strand, within Pf1N1B4_3271 at 3662.273 kb on - strand, within Pf1N1B4_3271 at 3662.273 kb on - strand, within Pf1N1B4_3271 at 3662.273 kb on - strand, within Pf1N1B4_3271 at 3662.314 kb on - strand at 3662.347 kb on + strand at 3662.347 kb on + strand at 3662.347 kb on + strand at 3662.347 kb on + strand at 3662.347 kb on + strand at 3662.347 kb on + strand at 3662.348 kb on - strand at 3662.348 kb on - strand at 3662.348 kb on - strand at 3662.348 kb on - strand at 3662.348 kb on - strand at 3662.348 kb on - strand at 3662.348 kb on - strand at 3662.348 kb on - strand at 3662.348 kb on - strand at 3662.460 kb on - strand, within Pf1N1B4_3272 at 3662.499 kb on + strand, within Pf1N1B4_3272 at 3662.707 kb on + strand, within Pf1N1B4_3273 at 3662.707 kb on + strand, within Pf1N1B4_3273 at 3662.708 kb on - strand, within Pf1N1B4_3273 at 3662.708 kb on - strand, within Pf1N1B4_3273 at 3662.708 kb on - strand, within Pf1N1B4_3273 at 3663.139 kb on + strand at 3663.139 kb on + strand at 3663.140 kb on - strand at 3663.140 kb on - strand at 3663.140 kb on - strand at 3663.140 kb on - strand at 3663.140 kb on - strand at 3663.156 kb on - strand at 3663.156 kb on - strand at 3663.162 kb on + strand at 3663.162 kb on + strand at 3663.163 kb on - strand at 3663.163 kb on - strand at 3663.163 kb on - strand at 3663.184 kb on + strand at 3663.184 kb on + strand at 3663.186 kb on + strand at 3663.186 kb on + strand at 3663.186 kb on + strand at 3663.186 kb on + strand at 3663.186 kb on + strand at 3663.187 kb on - strand at 3663.187 kb on - strand at 3663.187 kb on - strand at 3663.187 kb on - strand at 3663.187 kb on - strand at 3663.187 kb on - strand at 3663.191 kb on + strand at 3663.289 kb on + strand, within Pf1N1B4_3274 at 3663.289 kb on + strand, within Pf1N1B4_3274 at 3663.290 kb on - strand, within Pf1N1B4_3274 at 3663.290 kb on - strand, within Pf1N1B4_3274 at 3663.290 kb on - strand, within Pf1N1B4_3274 at 3663.315 kb on + strand, within Pf1N1B4_3274 at 3663.316 kb on - strand, within Pf1N1B4_3274 at 3663.387 kb on + strand, within Pf1N1B4_3274 at 3663.387 kb on + strand, within Pf1N1B4_3274 at 3663.388 kb on - strand, within Pf1N1B4_3274 at 3663.502 kb on - strand, within Pf1N1B4_3274 at 3663.502 kb on - strand at 3663.586 kb on - strand at 3663.640 kb on + strand at 3663.640 kb on + strand at 3663.640 kb on + strand at 3663.641 kb on - strand at 3663.641 kb on - strand at 3663.727 kb on + strand at 3663.727 kb on + strand at 3663.727 kb on + strand at 3663.728 kb on - strand at 3663.728 kb on - strand at 3663.866 kb on + strand, within Pf1N1B4_3275
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 1mM remove 3,661,660 + Pf1N1B4_3271 0.35 +1.7 3,661,661 - Pf1N1B4_3271 0.35 +0.0 3,661,661 - Pf1N1B4_3271 0.35 +1.6 3,661,828 + Pf1N1B4_3271 0.49 -0.1 3,661,931 - Pf1N1B4_3271 0.58 -0.4 3,661,931 - Pf1N1B4_3271 0.58 -1.4 3,661,931 - Pf1N1B4_3271 0.58 +0.9 3,661,931 - Pf1N1B4_3271 0.58 +3.1 3,661,931 - Pf1N1B4_3271 0.58 +0.1 3,661,979 - Pf1N1B4_3271 0.62 -0.3 3,662,179 + Pf1N1B4_3271 0.80 +3.2 3,662,179 + Pf1N1B4_3271 0.80 -1.6 3,662,179 + Pf1N1B4_3271 0.80 -1.7 3,662,180 - Pf1N1B4_3271 0.80 +1.0 3,662,180 - Pf1N1B4_3271 0.80 -1.3 3,662,180 - +0.8 3,662,180 - Pf1N1B4_3271 0.80 +4.1 3,662,180 - Pf1N1B4_3271 0.80 +1.4 3,662,184 - Pf1N1B4_3271 0.80 -0.8 3,662,272 + Pf1N1B4_3271 0.87 +1.7 3,662,272 + Pf1N1B4_3271 0.87 -2.6 3,662,272 + Pf1N1B4_3271 0.87 -0.3 3,662,273 - Pf1N1B4_3271 0.88 -2.2 3,662,273 - Pf1N1B4_3271 0.88 -0.0 3,662,273 - Pf1N1B4_3271 0.88 +1.8 3,662,314 - -0.8 3,662,347 + -1.8 3,662,347 + +1.7 3,662,347 + -1.2 3,662,347 + -0.9 3,662,347 + -1.0 3,662,347 + +2.7 3,662,348 - +0.2 3,662,348 - +2.1 3,662,348 - -1.2 3,662,348 - -2.0 3,662,348 - -2.3 3,662,348 - +1.0 3,662,348 - -1.7 3,662,348 - +0.3 3,662,348 - -0.8 3,662,460 - Pf1N1B4_3272 0.20 -0.3 3,662,499 + Pf1N1B4_3272 0.53 -0.6 3,662,707 + Pf1N1B4_3273 0.28 -2.0 3,662,707 + Pf1N1B4_3273 0.28 -1.3 3,662,708 - Pf1N1B4_3273 0.29 -1.9 3,662,708 - Pf1N1B4_3273 0.29 -1.7 3,662,708 - Pf1N1B4_3273 0.29 -1.3 3,663,139 + -1.3 3,663,139 + +1.2 3,663,140 - -3.1 3,663,140 - +1.5 3,663,140 - -0.9 3,663,140 - -2.6 3,663,140 - +1.8 3,663,156 - +1.2 3,663,156 - -2.0 3,663,162 + -0.1 3,663,162 + -1.3 3,663,163 - -0.5 3,663,163 - +1.0 3,663,163 - -2.2 3,663,184 + +0.2 3,663,184 + -0.7 3,663,186 + -1.6 3,663,186 + +1.1 3,663,186 + +2.9 3,663,186 + -1.1 3,663,186 + -0.1 3,663,187 - -2.1 3,663,187 - -1.2 3,663,187 - +1.4 3,663,187 - -1.1 3,663,187 - +3.0 3,663,187 - -1.3 3,663,191 + +0.4 3,663,289 + Pf1N1B4_3274 0.21 +1.0 3,663,289 + Pf1N1B4_3274 0.21 -2.5 3,663,290 - Pf1N1B4_3274 0.21 +2.9 3,663,290 - Pf1N1B4_3274 0.21 -1.0 3,663,290 - Pf1N1B4_3274 0.21 -0.7 3,663,315 + Pf1N1B4_3274 0.27 +1.1 3,663,316 - Pf1N1B4_3274 0.28 -2.9 3,663,387 + Pf1N1B4_3274 0.45 -1.7 3,663,387 + Pf1N1B4_3274 0.45 -0.3 3,663,388 - Pf1N1B4_3274 0.45 -0.7 3,663,502 - Pf1N1B4_3274 0.72 -0.0 3,663,502 - -2.0 3,663,586 - +1.7 3,663,640 + -0.9 3,663,640 + +2.5 3,663,640 + -2.0 3,663,641 - -0.5 3,663,641 - -2.7 3,663,727 + -1.3 3,663,727 + -0.3 3,663,727 + +0.9 3,663,728 - +1.4 3,663,728 - -1.5 3,663,866 + Pf1N1B4_3275 0.29 -1.4
Or see this region's nucleotide sequence