Experiment: carbon source Sucrose 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2624 and Pf1N1B4_2625 are separated by 54 nucleotides Pf1N1B4_2625 and Pf1N1B4_2626 overlap by 39 nucleotides Pf1N1B4_2626 and Pf1N1B4_2627 are separated by 12 nucleotides Pf1N1B4_2627 and Pf1N1B4_2628 overlap by 17 nucleotides Pf1N1B4_2628 and Pf1N1B4_2629 are separated by 17 nucleotides
Pf1N1B4_2624 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 2,963,494 to 2,964,489
_2624
Pf1N1B4_2625 - hypothetical protein, at 2,964,544 to 2,964,834
_2625
Pf1N1B4_2626 - Biotin carboxylase (EC 6.3.4.14), at 2,964,796 to 2,965,881
_2626
Pf1N1B4_2627 - Prolyl oligopeptidase family protein, at 2,965,894 to 2,966,652
_2627
Pf1N1B4_2628 - hypothetical protein, at 2,966,636 to 2,966,764
_2628
Pf1N1B4_2629 - Inner membrane protein YbhQ, at 2,966,782 to 2,967,768
_2629
Position (kb)
2964
2965
2966 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2963.844 kb on + strand, within Pf1N1B4_2624 at 2963.844 kb on + strand, within Pf1N1B4_2624 at 2963.844 kb on + strand, within Pf1N1B4_2624 at 2963.844 kb on + strand, within Pf1N1B4_2624 at 2963.844 kb on + strand, within Pf1N1B4_2624 at 2963.844 kb on + strand, within Pf1N1B4_2624 at 2963.844 kb on + strand, within Pf1N1B4_2624 at 2963.845 kb on - strand, within Pf1N1B4_2624 at 2963.845 kb on - strand, within Pf1N1B4_2624 at 2963.845 kb on - strand, within Pf1N1B4_2624 at 2963.845 kb on - strand, within Pf1N1B4_2624 at 2963.845 kb on - strand, within Pf1N1B4_2624 at 2963.845 kb on - strand, within Pf1N1B4_2624 at 2963.845 kb on - strand, within Pf1N1B4_2624 at 2964.020 kb on + strand, within Pf1N1B4_2624 at 2964.021 kb on - strand, within Pf1N1B4_2624 at 2964.021 kb on - strand, within Pf1N1B4_2624 at 2964.021 kb on - strand, within Pf1N1B4_2624 at 2964.118 kb on - strand, within Pf1N1B4_2624 at 2964.494 kb on + strand at 2964.495 kb on - strand at 2964.495 kb on - strand at 2964.542 kb on + strand at 2964.643 kb on + strand, within Pf1N1B4_2625 at 2964.644 kb on - strand, within Pf1N1B4_2625 at 2964.644 kb on - strand, within Pf1N1B4_2625 at 2964.665 kb on + strand, within Pf1N1B4_2625 at 2964.666 kb on - strand, within Pf1N1B4_2625 at 2964.666 kb on - strand, within Pf1N1B4_2625 at 2964.666 kb on - strand, within Pf1N1B4_2625 at 2964.690 kb on - strand, within Pf1N1B4_2625 at 2964.763 kb on + strand, within Pf1N1B4_2625 at 2964.763 kb on + strand, within Pf1N1B4_2625 at 2964.763 kb on + strand, within Pf1N1B4_2625 at 2964.763 kb on + strand, within Pf1N1B4_2625 at 2964.763 kb on + strand, within Pf1N1B4_2625 at 2964.764 kb on - strand, within Pf1N1B4_2625 at 2964.764 kb on - strand, within Pf1N1B4_2625 at 2964.764 kb on - strand, within Pf1N1B4_2625 at 2964.764 kb on - strand, within Pf1N1B4_2625 at 2964.764 kb on - strand, within Pf1N1B4_2625 at 2964.931 kb on + strand, within Pf1N1B4_2626 at 2964.931 kb on + strand, within Pf1N1B4_2626 at 2964.932 kb on - strand, within Pf1N1B4_2626 at 2964.932 kb on - strand, within Pf1N1B4_2626 at 2964.932 kb on - strand, within Pf1N1B4_2626 at 2964.932 kb on - strand, within Pf1N1B4_2626 at 2964.932 kb on - strand, within Pf1N1B4_2626 at 2964.964 kb on + strand, within Pf1N1B4_2626 at 2964.964 kb on + strand, within Pf1N1B4_2626 at 2964.964 kb on + strand, within Pf1N1B4_2626 at 2964.965 kb on - strand, within Pf1N1B4_2626 at 2964.965 kb on - strand, within Pf1N1B4_2626 at 2964.965 kb on - strand, within Pf1N1B4_2626 at 2964.965 kb on - strand, within Pf1N1B4_2626 at 2965.072 kb on - strand, within Pf1N1B4_2626 at 2965.225 kb on + strand, within Pf1N1B4_2626 at 2965.225 kb on + strand, within Pf1N1B4_2626 at 2965.225 kb on + strand, within Pf1N1B4_2626 at 2965.226 kb on - strand, within Pf1N1B4_2626 at 2965.226 kb on - strand, within Pf1N1B4_2626 at 2965.226 kb on - strand, within Pf1N1B4_2626 at 2965.447 kb on + strand, within Pf1N1B4_2626 at 2965.447 kb on + strand, within Pf1N1B4_2626 at 2965.447 kb on + strand, within Pf1N1B4_2626 at 2965.447 kb on + strand, within Pf1N1B4_2626 at 2965.447 kb on + strand, within Pf1N1B4_2626 at 2965.447 kb on + strand, within Pf1N1B4_2626 at 2965.447 kb on + strand, within Pf1N1B4_2626 at 2965.448 kb on - strand, within Pf1N1B4_2626 at 2965.448 kb on - strand, within Pf1N1B4_2626 at 2965.448 kb on - strand at 2965.448 kb on - strand at 2965.448 kb on - strand, within Pf1N1B4_2626 at 2965.448 kb on - strand, within Pf1N1B4_2626 at 2965.513 kb on + strand, within Pf1N1B4_2626 at 2965.514 kb on - strand, within Pf1N1B4_2626 at 2965.514 kb on - strand, within Pf1N1B4_2626 at 2965.516 kb on + strand, within Pf1N1B4_2626 at 2965.516 kb on + strand, within Pf1N1B4_2626 at 2965.516 kb on + strand, within Pf1N1B4_2626 at 2965.516 kb on + strand, within Pf1N1B4_2626 at 2965.517 kb on - strand, within Pf1N1B4_2626 at 2965.517 kb on - strand, within Pf1N1B4_2626 at 2965.517 kb on - strand, within Pf1N1B4_2626 at 2965.618 kb on + strand, within Pf1N1B4_2626 at 2965.618 kb on + strand, within Pf1N1B4_2626 at 2965.618 kb on + strand at 2965.636 kb on + strand, within Pf1N1B4_2626 at 2965.636 kb on + strand, within Pf1N1B4_2626 at 2965.636 kb on + strand, within Pf1N1B4_2626 at 2965.636 kb on + strand, within Pf1N1B4_2626 at 2965.636 kb on + strand, within Pf1N1B4_2626 at 2965.637 kb on - strand, within Pf1N1B4_2626 at 2965.637 kb on - strand, within Pf1N1B4_2626 at 2965.637 kb on - strand, within Pf1N1B4_2626 at 2965.637 kb on - strand, within Pf1N1B4_2626 at 2965.637 kb on - strand, within Pf1N1B4_2626 at 2965.798 kb on + strand at 2965.798 kb on + strand at 2965.799 kb on - strand at 2965.799 kb on - strand at 2965.799 kb on - strand at 2965.799 kb on - strand at 2965.854 kb on + strand at 2965.854 kb on + strand at 2965.854 kb on + strand at 2965.855 kb on - strand at 2965.855 kb on - strand at 2965.855 kb on - strand at 2965.875 kb on + strand at 2965.875 kb on + strand at 2965.875 kb on + strand at 2965.875 kb on + strand at 2965.875 kb on + strand at 2965.876 kb on - strand at 2965.876 kb on - strand at 2965.876 kb on - strand at 2965.876 kb on - strand at 2965.879 kb on + strand at 2965.880 kb on - strand at 2965.893 kb on + strand at 2965.995 kb on - strand, within Pf1N1B4_2627 at 2966.224 kb on + strand, within Pf1N1B4_2627 at 2966.225 kb on - strand, within Pf1N1B4_2627 at 2966.234 kb on - strand, within Pf1N1B4_2627 at 2966.299 kb on + strand, within Pf1N1B4_2627 at 2966.458 kb on + strand, within Pf1N1B4_2627 at 2966.458 kb on + strand, within Pf1N1B4_2627 at 2966.458 kb on + strand, within Pf1N1B4_2627 at 2966.458 kb on + strand, within Pf1N1B4_2627 at 2966.458 kb on + strand, within Pf1N1B4_2627 at 2966.458 kb on + strand, within Pf1N1B4_2627 at 2966.458 kb on + strand, within Pf1N1B4_2627 at 2966.459 kb on - strand, within Pf1N1B4_2627 at 2966.459 kb on - strand, within Pf1N1B4_2627 at 2966.459 kb on - strand, within Pf1N1B4_2627 at 2966.459 kb on - strand, within Pf1N1B4_2627 at 2966.459 kb on - strand, within Pf1N1B4_2627 at 2966.459 kb on - strand, within Pf1N1B4_2627 at 2966.459 kb on - strand, within Pf1N1B4_2627 at 2966.459 kb on - strand, within Pf1N1B4_2627 at 2966.459 kb on - strand at 2966.578 kb on + strand at 2966.578 kb on + strand at 2966.578 kb on + strand at 2966.578 kb on + strand at 2966.578 kb on + strand at 2966.578 kb on + strand at 2966.578 kb on + strand at 2966.578 kb on + strand at 2966.579 kb on - strand at 2966.579 kb on - strand at 2966.579 kb on - strand at 2966.579 kb on - strand at 2966.579 kb on - strand at 2966.637 kb on + strand at 2966.638 kb on - strand at 2966.837 kb on + strand at 2966.837 kb on + strand at 2966.837 kb on + strand at 2966.837 kb on + strand at 2966.837 kb on + strand at 2966.837 kb on + strand at 2966.837 kb on + strand at 2966.838 kb on - strand at 2966.838 kb on - strand at 2966.851 kb on - strand at 2966.873 kb on + strand at 2966.873 kb on + strand at 2966.874 kb on - strand at 2966.874 kb on - strand at 2966.874 kb on - strand at 2966.874 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 1mM remove 2,963,844 + Pf1N1B4_2624 0.35 -0.4 2,963,844 + Pf1N1B4_2624 0.35 +1.3 2,963,844 + Pf1N1B4_2624 0.35 +1.4 2,963,844 + Pf1N1B4_2624 0.35 -0.9 2,963,844 + Pf1N1B4_2624 0.35 +0.5 2,963,844 + Pf1N1B4_2624 0.35 +2.1 2,963,844 + Pf1N1B4_2624 0.35 -2.1 2,963,845 - Pf1N1B4_2624 0.35 -1.9 2,963,845 - Pf1N1B4_2624 0.35 +1.1 2,963,845 - Pf1N1B4_2624 0.35 -3.1 2,963,845 - Pf1N1B4_2624 0.35 -2.2 2,963,845 - Pf1N1B4_2624 0.35 +2.4 2,963,845 - Pf1N1B4_2624 0.35 +1.2 2,963,845 - Pf1N1B4_2624 0.35 -1.9 2,964,020 + Pf1N1B4_2624 0.53 +0.3 2,964,021 - Pf1N1B4_2624 0.53 +0.5 2,964,021 - Pf1N1B4_2624 0.53 +1.5 2,964,021 - Pf1N1B4_2624 0.53 -1.1 2,964,118 - Pf1N1B4_2624 0.63 +0.3 2,964,494 + -0.6 2,964,495 - -1.7 2,964,495 - -1.1 2,964,542 + +0.6 2,964,643 + Pf1N1B4_2625 0.34 +2.6 2,964,644 - Pf1N1B4_2625 0.34 -1.6 2,964,644 - Pf1N1B4_2625 0.34 -0.4 2,964,665 + Pf1N1B4_2625 0.42 +1.2 2,964,666 - Pf1N1B4_2625 0.42 -1.1 2,964,666 - Pf1N1B4_2625 0.42 +0.3 2,964,666 - Pf1N1B4_2625 0.42 -1.7 2,964,690 - Pf1N1B4_2625 0.50 +1.9 2,964,763 + Pf1N1B4_2625 0.75 +0.3 2,964,763 + Pf1N1B4_2625 0.75 +2.0 2,964,763 + Pf1N1B4_2625 0.75 -1.5 2,964,763 + Pf1N1B4_2625 0.75 +0.3 2,964,763 + Pf1N1B4_2625 0.75 +1.0 2,964,764 - Pf1N1B4_2625 0.76 -1.9 2,964,764 - Pf1N1B4_2625 0.76 -1.2 2,964,764 - Pf1N1B4_2625 0.76 -0.4 2,964,764 - Pf1N1B4_2625 0.76 -0.2 2,964,764 - Pf1N1B4_2625 0.76 +0.3 2,964,931 + Pf1N1B4_2626 0.12 -2.2 2,964,931 + Pf1N1B4_2626 0.12 -0.1 2,964,932 - Pf1N1B4_2626 0.13 +0.3 2,964,932 - Pf1N1B4_2626 0.13 +1.5 2,964,932 - Pf1N1B4_2626 0.13 -0.7 2,964,932 - Pf1N1B4_2626 0.13 +0.0 2,964,932 - Pf1N1B4_2626 0.13 +3.4 2,964,964 + Pf1N1B4_2626 0.15 +2.6 2,964,964 + Pf1N1B4_2626 0.15 +2.2 2,964,964 + Pf1N1B4_2626 0.15 -1.0 2,964,965 - Pf1N1B4_2626 0.16 -1.5 2,964,965 - Pf1N1B4_2626 0.16 -1.4 2,964,965 - Pf1N1B4_2626 0.16 -0.8 2,964,965 - Pf1N1B4_2626 0.16 -1.0 2,965,072 - Pf1N1B4_2626 0.25 -3.0 2,965,225 + Pf1N1B4_2626 0.40 +0.1 2,965,225 + Pf1N1B4_2626 0.40 -1.3 2,965,225 + Pf1N1B4_2626 0.40 +1.9 2,965,226 - Pf1N1B4_2626 0.40 -1.3 2,965,226 - Pf1N1B4_2626 0.40 -0.1 2,965,226 - Pf1N1B4_2626 0.40 -2.6 2,965,447 + Pf1N1B4_2626 0.60 +2.6 2,965,447 + Pf1N1B4_2626 0.60 +1.0 2,965,447 + Pf1N1B4_2626 0.60 +0.6 2,965,447 + Pf1N1B4_2626 0.60 +0.3 2,965,447 + Pf1N1B4_2626 0.60 -0.3 2,965,447 + Pf1N1B4_2626 0.60 -0.1 2,965,447 + Pf1N1B4_2626 0.60 -1.2 2,965,448 - Pf1N1B4_2626 0.60 +2.6 2,965,448 - Pf1N1B4_2626 0.60 -1.0 2,965,448 - +0.6 2,965,448 - -0.7 2,965,448 - Pf1N1B4_2626 0.60 -1.7 2,965,448 - Pf1N1B4_2626 0.60 -1.3 2,965,513 + Pf1N1B4_2626 0.66 +1.7 2,965,514 - Pf1N1B4_2626 0.66 +0.0 2,965,514 - Pf1N1B4_2626 0.66 -1.5 2,965,516 + Pf1N1B4_2626 0.66 +0.2 2,965,516 + Pf1N1B4_2626 0.66 +0.0 2,965,516 + Pf1N1B4_2626 0.66 -0.9 2,965,516 + Pf1N1B4_2626 0.66 -3.0 2,965,517 - Pf1N1B4_2626 0.66 +0.8 2,965,517 - Pf1N1B4_2626 0.66 +2.7 2,965,517 - Pf1N1B4_2626 0.66 +1.3 2,965,618 + Pf1N1B4_2626 0.76 -2.1 2,965,618 + Pf1N1B4_2626 0.76 -1.1 2,965,618 + -1.0 2,965,636 + Pf1N1B4_2626 0.77 -0.1 2,965,636 + Pf1N1B4_2626 0.77 +2.0 2,965,636 + Pf1N1B4_2626 0.77 -0.1 2,965,636 + Pf1N1B4_2626 0.77 -0.2 2,965,636 + Pf1N1B4_2626 0.77 +0.1 2,965,637 - Pf1N1B4_2626 0.77 -0.0 2,965,637 - Pf1N1B4_2626 0.77 +1.4 2,965,637 - Pf1N1B4_2626 0.77 -1.9 2,965,637 - Pf1N1B4_2626 0.77 +3.4 2,965,637 - Pf1N1B4_2626 0.77 +1.5 2,965,798 + -1.9 2,965,798 + -1.0 2,965,799 - +0.4 2,965,799 - +1.7 2,965,799 - -2.0 2,965,799 - +1.5 2,965,854 + -1.7 2,965,854 + +0.4 2,965,854 + +2.2 2,965,855 - -1.1 2,965,855 - +0.9 2,965,855 - +0.1 2,965,875 + +1.1 2,965,875 + -0.3 2,965,875 + +0.2 2,965,875 + -0.6 2,965,875 + +1.9 2,965,876 - +1.0 2,965,876 - +1.8 2,965,876 - +1.4 2,965,876 - +0.1 2,965,879 + -0.9 2,965,880 - +0.1 2,965,893 + -0.7 2,965,995 - Pf1N1B4_2627 0.13 -1.9 2,966,224 + Pf1N1B4_2627 0.43 -2.8 2,966,225 - Pf1N1B4_2627 0.44 -0.7 2,966,234 - Pf1N1B4_2627 0.45 +1.0 2,966,299 + Pf1N1B4_2627 0.53 +0.6 2,966,458 + Pf1N1B4_2627 0.74 +1.0 2,966,458 + Pf1N1B4_2627 0.74 -1.6 2,966,458 + Pf1N1B4_2627 0.74 +1.2 2,966,458 + Pf1N1B4_2627 0.74 -0.7 2,966,458 + Pf1N1B4_2627 0.74 -0.9 2,966,458 + Pf1N1B4_2627 0.74 +3.1 2,966,458 + Pf1N1B4_2627 0.74 -2.4 2,966,459 - Pf1N1B4_2627 0.74 +1.8 2,966,459 - Pf1N1B4_2627 0.74 +1.9 2,966,459 - Pf1N1B4_2627 0.74 +1.1 2,966,459 - Pf1N1B4_2627 0.74 +0.0 2,966,459 - Pf1N1B4_2627 0.74 -0.1 2,966,459 - Pf1N1B4_2627 0.74 -2.0 2,966,459 - Pf1N1B4_2627 0.74 +1.0 2,966,459 - Pf1N1B4_2627 0.74 +2.1 2,966,459 - -1.1 2,966,578 + -1.7 2,966,578 + -1.9 2,966,578 + +2.7 2,966,578 + -2.6 2,966,578 + +0.3 2,966,578 + +2.0 2,966,578 + -0.7 2,966,578 + +0.0 2,966,579 - +0.3 2,966,579 - +0.8 2,966,579 - -0.2 2,966,579 - -0.6 2,966,579 - -0.9 2,966,637 + +0.4 2,966,638 - -1.2 2,966,837 + -0.7 2,966,837 + +1.9 2,966,837 + -2.2 2,966,837 + -0.3 2,966,837 + -1.9 2,966,837 + +0.7 2,966,837 + -1.9 2,966,838 - +0.6 2,966,838 - +0.9 2,966,851 - +1.7 2,966,873 + -0.0 2,966,873 + +4.2 2,966,874 - -1.4 2,966,874 - -2.4 2,966,874 - -1.0 2,966,874 - -1.7
Or see this region's nucleotide sequence