Experiment: carbon source Sucrose 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2365 and Pf1N1B4_2366 overlap by 4 nucleotides Pf1N1B4_2366 and Pf1N1B4_2367 overlap by 4 nucleotides Pf1N1B4_2367 and Pf1N1B4_2368 are separated by 66 nucleotides Pf1N1B4_2368 and Pf1N1B4_2369 are separated by 1 nucleotides
Pf1N1B4_2365 - Malonate decarboxylase gamma subunit, at 2,682,226 to 2,683,029
_2365
Pf1N1B4_2366 - Phosphoribosyl-dephospho-CoA transferase (EC 2.7.7.-), at 2,683,026 to 2,683,643
_2366
Pf1N1B4_2367 - Malonyl CoA acyl carrier protein transacylase (EC 2.3.1.39), at 2,683,640 to 2,684,551
_2367
Pf1N1B4_2368 - Malonate transporter, MadL subunit, at 2,684,618 to 2,685,034
_2368
Pf1N1B4_2369 - Malonate transporter, MadM subunit, at 2,685,036 to 2,685,800
_2369
Position (kb)
2683
2684
2685 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 2682.808 kb on + strand, within Pf1N1B4_2365 at 2682.808 kb on + strand, within Pf1N1B4_2365 at 2682.808 kb on + strand, within Pf1N1B4_2365 at 2682.809 kb on - strand, within Pf1N1B4_2365 at 2683.246 kb on + strand, within Pf1N1B4_2366 at 2683.246 kb on + strand, within Pf1N1B4_2366 at 2683.527 kb on + strand, within Pf1N1B4_2366 at 2683.537 kb on + strand, within Pf1N1B4_2366 at 2683.537 kb on + strand, within Pf1N1B4_2366 at 2683.537 kb on + strand, within Pf1N1B4_2366 at 2683.538 kb on - strand, within Pf1N1B4_2366 at 2683.538 kb on - strand, within Pf1N1B4_2366 at 2683.538 kb on - strand, within Pf1N1B4_2366 at 2683.538 kb on - strand, within Pf1N1B4_2366 at 2683.800 kb on + strand, within Pf1N1B4_2367 at 2683.800 kb on + strand, within Pf1N1B4_2367 at 2683.800 kb on + strand, within Pf1N1B4_2367 at 2683.800 kb on + strand at 2683.801 kb on - strand, within Pf1N1B4_2367 at 2683.856 kb on + strand, within Pf1N1B4_2367 at 2683.857 kb on - strand, within Pf1N1B4_2367 at 2683.982 kb on + strand, within Pf1N1B4_2367 at 2683.982 kb on + strand, within Pf1N1B4_2367 at 2683.983 kb on - strand, within Pf1N1B4_2367 at 2684.078 kb on - strand, within Pf1N1B4_2367 at 2684.309 kb on - strand, within Pf1N1B4_2367 at 2684.379 kb on + strand, within Pf1N1B4_2367 at 2684.403 kb on + strand, within Pf1N1B4_2367 at 2684.404 kb on - strand, within Pf1N1B4_2367 at 2684.404 kb on - strand, within Pf1N1B4_2367 at 2684.409 kb on + strand, within Pf1N1B4_2367 at 2684.410 kb on - strand, within Pf1N1B4_2367 at 2684.410 kb on - strand, within Pf1N1B4_2367 at 2684.410 kb on - strand, within Pf1N1B4_2367 at 2684.506 kb on + strand at 2684.506 kb on + strand at 2684.507 kb on - strand at 2684.549 kb on + strand at 2684.549 kb on + strand at 2684.549 kb on + strand at 2684.549 kb on + strand at 2684.549 kb on + strand at 2684.550 kb on - strand at 2684.574 kb on + strand at 2684.575 kb on - strand at 2684.611 kb on + strand at 2684.611 kb on + strand at 2684.611 kb on + strand at 2684.611 kb on + strand at 2684.611 kb on + strand at 2684.612 kb on - strand at 2684.623 kb on + strand at 2684.623 kb on + strand at 2684.623 kb on + strand at 2684.624 kb on - strand at 2684.624 kb on - strand at 2684.624 kb on - strand at 2684.624 kb on - strand at 2684.627 kb on + strand at 2684.627 kb on + strand at 2684.627 kb on + strand at 2684.628 kb on - strand at 2684.653 kb on + strand at 2684.653 kb on + strand at 2684.828 kb on + strand, within Pf1N1B4_2368 at 2684.828 kb on + strand, within Pf1N1B4_2368 at 2684.828 kb on + strand, within Pf1N1B4_2368 at 2684.828 kb on + strand, within Pf1N1B4_2368 at 2684.829 kb on - strand, within Pf1N1B4_2368 at 2684.829 kb on - strand, within Pf1N1B4_2368 at 2684.829 kb on - strand, within Pf1N1B4_2368 at 2684.829 kb on - strand, within Pf1N1B4_2368 at 2684.829 kb on - strand, within Pf1N1B4_2368 at 2684.829 kb on - strand, within Pf1N1B4_2368 at 2684.999 kb on - strand at 2685.000 kb on + strand at 2685.000 kb on + strand at 2685.000 kb on + strand at 2685.000 kb on + strand at 2685.000 kb on + strand at 2685.001 kb on - strand at 2685.001 kb on - strand at 2685.035 kb on + strand at 2685.035 kb on + strand at 2685.035 kb on + strand at 2685.036 kb on - strand at 2685.036 kb on - strand at 2685.036 kb on - strand at 2685.068 kb on + strand at 2685.068 kb on + strand at 2685.477 kb on + strand, within Pf1N1B4_2369 at 2685.477 kb on + strand, within Pf1N1B4_2369 at 2685.477 kb on + strand, within Pf1N1B4_2369 at 2685.477 kb on + strand, within Pf1N1B4_2369 at 2685.478 kb on - strand, within Pf1N1B4_2369 at 2685.478 kb on - strand, within Pf1N1B4_2369 at 2685.478 kb on - strand, within Pf1N1B4_2369
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 1mM remove 2,682,808 + Pf1N1B4_2365 0.72 -1.8 2,682,808 + Pf1N1B4_2365 0.72 +0.5 2,682,808 + Pf1N1B4_2365 0.72 +1.1 2,682,809 - Pf1N1B4_2365 0.73 +2.4 2,683,246 + Pf1N1B4_2366 0.36 +0.5 2,683,246 + Pf1N1B4_2366 0.36 +0.3 2,683,527 + Pf1N1B4_2366 0.81 -1.2 2,683,537 + Pf1N1B4_2366 0.83 -0.9 2,683,537 + Pf1N1B4_2366 0.83 -1.0 2,683,537 + Pf1N1B4_2366 0.83 +0.1 2,683,538 - Pf1N1B4_2366 0.83 +1.9 2,683,538 - Pf1N1B4_2366 0.83 +0.7 2,683,538 - Pf1N1B4_2366 0.83 -0.1 2,683,538 - Pf1N1B4_2366 0.83 -1.2 2,683,800 + Pf1N1B4_2367 0.18 -0.1 2,683,800 + Pf1N1B4_2367 0.18 +0.8 2,683,800 + Pf1N1B4_2367 0.18 -0.9 2,683,800 + +0.2 2,683,801 - Pf1N1B4_2367 0.18 -0.3 2,683,856 + Pf1N1B4_2367 0.24 -1.8 2,683,857 - Pf1N1B4_2367 0.24 +0.0 2,683,982 + Pf1N1B4_2367 0.38 +0.7 2,683,982 + Pf1N1B4_2367 0.38 +1.7 2,683,983 - Pf1N1B4_2367 0.38 -1.0 2,684,078 - Pf1N1B4_2367 0.48 -1.0 2,684,309 - Pf1N1B4_2367 0.73 -0.7 2,684,379 + Pf1N1B4_2367 0.81 +1.8 2,684,403 + Pf1N1B4_2367 0.84 +2.3 2,684,404 - Pf1N1B4_2367 0.84 -2.0 2,684,404 - Pf1N1B4_2367 0.84 -2.1 2,684,409 + Pf1N1B4_2367 0.84 -2.1 2,684,410 - Pf1N1B4_2367 0.84 +0.2 2,684,410 - Pf1N1B4_2367 0.84 +0.1 2,684,410 - Pf1N1B4_2367 0.84 +1.7 2,684,506 + +0.4 2,684,506 + +0.2 2,684,507 - +2.4 2,684,549 + -1.9 2,684,549 + -0.1 2,684,549 + +2.7 2,684,549 + -2.3 2,684,549 + +0.4 2,684,550 - -0.8 2,684,574 + +0.2 2,684,575 - -0.8 2,684,611 + +1.6 2,684,611 + -1.2 2,684,611 + -2.0 2,684,611 + +0.6 2,684,611 + +2.7 2,684,612 - +2.2 2,684,623 + -2.3 2,684,623 + -0.1 2,684,623 + +0.1 2,684,624 - -1.4 2,684,624 - -2.0 2,684,624 - -1.5 2,684,624 - -0.3 2,684,627 + -0.9 2,684,627 + -0.1 2,684,627 + -0.9 2,684,628 - -1.5 2,684,653 + -1.1 2,684,653 + -2.2 2,684,828 + Pf1N1B4_2368 0.50 -0.9 2,684,828 + Pf1N1B4_2368 0.50 +2.3 2,684,828 + Pf1N1B4_2368 0.50 +1.1 2,684,828 + Pf1N1B4_2368 0.50 +2.7 2,684,829 - Pf1N1B4_2368 0.51 -0.9 2,684,829 - Pf1N1B4_2368 0.51 +0.7 2,684,829 - Pf1N1B4_2368 0.51 +1.0 2,684,829 - Pf1N1B4_2368 0.51 -0.8 2,684,829 - Pf1N1B4_2368 0.51 -0.9 2,684,829 - Pf1N1B4_2368 0.51 -0.1 2,684,999 - +2.3 2,685,000 + +0.4 2,685,000 + -1.7 2,685,000 + +1.5 2,685,000 + +5.3 2,685,000 + -1.7 2,685,001 - +1.1 2,685,001 - -0.9 2,685,035 + -0.1 2,685,035 + +2.3 2,685,035 + -2.0 2,685,036 - -0.0 2,685,036 - -1.7 2,685,036 - +0.6 2,685,068 + +1.3 2,685,068 + -0.9 2,685,477 + Pf1N1B4_2369 0.58 -0.1 2,685,477 + Pf1N1B4_2369 0.58 -0.4 2,685,477 + Pf1N1B4_2369 0.58 -1.0 2,685,477 + Pf1N1B4_2369 0.58 -0.8 2,685,478 - Pf1N1B4_2369 0.58 -0.1 2,685,478 - Pf1N1B4_2369 0.58 -1.1 2,685,478 - Pf1N1B4_2369 0.58 +1.5
Or see this region's nucleotide sequence