Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5629 and Pf1N1B4_5630 are separated by 20 nucleotides Pf1N1B4_5630 and Pf1N1B4_5631 are separated by 15 nucleotides Pf1N1B4_5631 and Pf1N1B4_5632 are separated by 305 nucleotides
Pf1N1B4_5629 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 56,358 to 57,932
_5629
Pf1N1B4_5630 - hypothetical protein, at 57,953 to 58,084
_5630
Pf1N1B4_5631 - Sigma-54 dependent transcriptional regulator, at 58,100 to 59,515
_5631
Pf1N1B4_5632 - D-beta-hydroxybutyrate permease, at 59,821 to 61,212
_5632
Position (kb) 58
59
60 Strain fitness (log2 ratio) -2
-1
0
1
2 at 57.106 kb on + strand, within Pf1N1B4_5629 at 57.107 kb on - strand, within Pf1N1B4_5629 at 57.110 kb on - strand, within Pf1N1B4_5629 at 57.229 kb on - strand, within Pf1N1B4_5629 at 57.512 kb on + strand, within Pf1N1B4_5629 at 57.769 kb on - strand, within Pf1N1B4_5629 at 57.894 kb on + strand at 58.002 kb on - strand, within Pf1N1B4_5630 at 58.078 kb on + strand at 58.078 kb on + strand at 58.079 kb on - strand at 58.082 kb on + strand at 58.082 kb on + strand at 58.083 kb on - strand at 58.083 kb on - strand at 58.083 kb on - strand at 58.142 kb on - strand at 58.557 kb on - strand, within Pf1N1B4_5631 at 58.557 kb on - strand, within Pf1N1B4_5631 at 58.557 kb on - strand, within Pf1N1B4_5631 at 58.732 kb on - strand, within Pf1N1B4_5631 at 58.732 kb on - strand, within Pf1N1B4_5631 at 58.732 kb on - strand, within Pf1N1B4_5631 at 59.048 kb on + strand, within Pf1N1B4_5631 at 59.251 kb on + strand, within Pf1N1B4_5631 at 59.251 kb on + strand, within Pf1N1B4_5631 at 59.252 kb on - strand, within Pf1N1B4_5631 at 59.474 kb on + strand at 59.475 kb on - strand at 59.525 kb on + strand at 59.525 kb on + strand at 59.526 kb on - strand at 60.454 kb on + strand, within Pf1N1B4_5632
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 57,106 + Pf1N1B4_5629 0.47 +0.3 57,107 - Pf1N1B4_5629 0.48 +0.6 57,110 - Pf1N1B4_5629 0.48 -2.7 57,229 - Pf1N1B4_5629 0.55 +0.2 57,512 + Pf1N1B4_5629 0.73 +0.9 57,769 - Pf1N1B4_5629 0.90 +0.8 57,894 + +1.4 58,002 - Pf1N1B4_5630 0.37 -0.4 58,078 + +0.0 58,078 + -2.7 58,079 - +0.9 58,082 + -0.9 58,082 + -0.1 58,083 - -2.6 58,083 - +1.1 58,083 - -0.9 58,142 - +2.5 58,557 - Pf1N1B4_5631 0.32 -1.4 58,557 - Pf1N1B4_5631 0.32 +0.7 58,557 - Pf1N1B4_5631 0.32 -0.3 58,732 - Pf1N1B4_5631 0.45 -1.2 58,732 - Pf1N1B4_5631 0.45 -2.3 58,732 - Pf1N1B4_5631 0.45 -0.5 59,048 + Pf1N1B4_5631 0.67 -2.6 59,251 + Pf1N1B4_5631 0.81 -1.6 59,251 + Pf1N1B4_5631 0.81 -1.7 59,252 - Pf1N1B4_5631 0.81 -0.0 59,474 + +0.4 59,475 - +1.0 59,525 + +0.6 59,525 + +0.7 59,526 - -0.1 60,454 + Pf1N1B4_5632 0.45 +0.3
Or see this region's nucleotide sequence