Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5504 and Pf1N1B4_5505 are separated by 38 nucleotides Pf1N1B4_5505 and Pf1N1B4_5506 overlap by 10 nucleotides Pf1N1B4_5506 and Pf1N1B4_5507 are separated by 48 nucleotides
Pf1N1B4_5504 - hypothetical protein, at 254,478 to 255,089
_5504
Pf1N1B4_5505 - hypothetical protein, at 255,128 to 255,304
_5505
Pf1N1B4_5506 - hypothetical protein, at 255,295 to 255,819
_5506
Pf1N1B4_5507 - hypothetical protein, at 255,868 to 256,542
_5507
Position (kb) 254
255
256 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 253.492 kb on - strand at 253.494 kb on + strand at 253.495 kb on - strand at 253.518 kb on + strand at 253.559 kb on + strand at 253.560 kb on - strand at 253.593 kb on - strand at 253.593 kb on - strand at 253.604 kb on - strand at 253.604 kb on - strand at 253.725 kb on + strand at 253.725 kb on + strand at 253.726 kb on - strand at 253.770 kb on + strand at 253.770 kb on + strand at 253.770 kb on + strand at 253.770 kb on + strand at 253.781 kb on + strand at 253.958 kb on - strand at 253.971 kb on - strand at 253.989 kb on + strand at 254.037 kb on + strand at 254.038 kb on - strand at 254.038 kb on - strand at 254.074 kb on + strand at 254.074 kb on + strand at 254.074 kb on + strand at 254.075 kb on - strand at 254.075 kb on - strand at 254.161 kb on - strand at 254.417 kb on + strand at 254.418 kb on - strand at 254.501 kb on - strand at 254.538 kb on + strand at 254.539 kb on - strand at 254.539 kb on - strand at 254.581 kb on + strand, within Pf1N1B4_5504 at 254.581 kb on + strand, within Pf1N1B4_5504 at 254.582 kb on - strand, within Pf1N1B4_5504 at 254.582 kb on - strand, within Pf1N1B4_5504 at 254.582 kb on - strand, within Pf1N1B4_5504 at 254.702 kb on - strand, within Pf1N1B4_5504 at 254.730 kb on - strand, within Pf1N1B4_5504 at 254.730 kb on - strand, within Pf1N1B4_5504 at 254.769 kb on + strand, within Pf1N1B4_5504 at 254.769 kb on - strand, within Pf1N1B4_5504 at 254.770 kb on - strand, within Pf1N1B4_5504 at 254.770 kb on - strand, within Pf1N1B4_5504 at 254.770 kb on - strand, within Pf1N1B4_5504 at 254.770 kb on - strand, within Pf1N1B4_5504 at 254.770 kb on - strand, within Pf1N1B4_5504 at 254.770 kb on - strand, within Pf1N1B4_5504 at 254.770 kb on - strand, within Pf1N1B4_5504 at 254.770 kb on - strand, within Pf1N1B4_5504 at 254.834 kb on - strand, within Pf1N1B4_5504 at 254.836 kb on - strand, within Pf1N1B4_5504 at 254.836 kb on - strand, within Pf1N1B4_5504 at 254.836 kb on - strand, within Pf1N1B4_5504 at 254.893 kb on + strand, within Pf1N1B4_5504 at 254.894 kb on - strand, within Pf1N1B4_5504 at 254.894 kb on - strand, within Pf1N1B4_5504 at 255.054 kb on + strand at 255.055 kb on - strand at 255.066 kb on + strand at 255.067 kb on - strand at 255.067 kb on - strand at 255.131 kb on + strand at 255.131 kb on + strand at 255.131 kb on + strand at 255.131 kb on + strand at 255.131 kb on + strand at 255.131 kb on + strand at 255.131 kb on + strand at 255.132 kb on - strand at 255.132 kb on - strand at 255.132 kb on - strand at 255.132 kb on - strand at 255.132 kb on - strand at 255.132 kb on - strand at 255.132 kb on - strand at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.242 kb on + strand at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.242 kb on + strand at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.243 kb on - strand, within Pf1N1B4_5505 at 255.243 kb on - strand at 255.269 kb on + strand at 255.269 kb on + strand at 255.269 kb on + strand, within Pf1N1B4_5505 at 255.269 kb on + strand, within Pf1N1B4_5505 at 255.270 kb on - strand, within Pf1N1B4_5505 at 255.270 kb on - strand, within Pf1N1B4_5505 at 255.289 kb on + strand at 255.592 kb on + strand, within Pf1N1B4_5506 at 255.592 kb on + strand, within Pf1N1B4_5506 at 255.592 kb on + strand, within Pf1N1B4_5506 at 255.592 kb on + strand, within Pf1N1B4_5506 at 255.592 kb on + strand at 255.592 kb on + strand, within Pf1N1B4_5506 at 255.593 kb on - strand, within Pf1N1B4_5506 at 255.593 kb on - strand, within Pf1N1B4_5506 at 255.593 kb on - strand at 255.593 kb on - strand at 255.689 kb on + strand, within Pf1N1B4_5506 at 255.689 kb on + strand, within Pf1N1B4_5506 at 255.689 kb on + strand, within Pf1N1B4_5506 at 255.689 kb on + strand, within Pf1N1B4_5506 at 255.691 kb on + strand at 255.692 kb on - strand, within Pf1N1B4_5506 at 255.740 kb on + strand, within Pf1N1B4_5506 at 255.741 kb on - strand, within Pf1N1B4_5506 at 255.741 kb on - strand, within Pf1N1B4_5506 at 255.829 kb on + strand at 255.829 kb on + strand at 255.829 kb on + strand at 255.830 kb on - strand at 255.881 kb on + strand at 255.881 kb on + strand at 255.881 kb on + strand at 255.882 kb on - strand at 255.932 kb on + strand at 255.933 kb on - strand at 255.933 kb on - strand at 255.933 kb on - strand at 255.933 kb on - strand at 255.933 kb on - strand at 255.933 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 253,492 - +1.7 253,494 + -1.4 253,495 - +1.3 253,518 + +2.3 253,559 + +2.9 253,560 - +0.5 253,593 - +0.3 253,593 - +1.5 253,604 - +1.2 253,604 - -1.6 253,725 + +2.8 253,725 + -2.2 253,726 - -2.9 253,770 + -0.2 253,770 + +0.9 253,770 + -1.8 253,770 + +0.0 253,781 + +0.7 253,958 - +0.9 253,971 - -0.5 253,989 + +0.6 254,037 + -2.1 254,038 - -2.3 254,038 - +3.3 254,074 + +3.0 254,074 + +0.9 254,074 + -2.3 254,075 - -1.6 254,075 - +0.4 254,161 - -1.0 254,417 + -1.2 254,418 - -1.9 254,501 - +0.4 254,538 + +0.2 254,539 - -1.9 254,539 - +0.3 254,581 + Pf1N1B4_5504 0.17 -0.0 254,581 + Pf1N1B4_5504 0.17 -3.0 254,582 - Pf1N1B4_5504 0.17 -0.1 254,582 - Pf1N1B4_5504 0.17 -1.7 254,582 - Pf1N1B4_5504 0.17 -1.0 254,702 - Pf1N1B4_5504 0.37 +1.1 254,730 - Pf1N1B4_5504 0.41 -2.2 254,730 - Pf1N1B4_5504 0.41 -2.4 254,769 + Pf1N1B4_5504 0.48 +1.9 254,769 - Pf1N1B4_5504 0.48 -0.9 254,770 - Pf1N1B4_5504 0.48 -1.5 254,770 - Pf1N1B4_5504 0.48 -2.4 254,770 - Pf1N1B4_5504 0.48 -3.1 254,770 - Pf1N1B4_5504 0.48 -1.6 254,770 - Pf1N1B4_5504 0.48 +1.2 254,770 - Pf1N1B4_5504 0.48 -0.5 254,770 - Pf1N1B4_5504 0.48 -0.8 254,770 - Pf1N1B4_5504 0.48 -2.0 254,834 - Pf1N1B4_5504 0.58 +0.7 254,836 - Pf1N1B4_5504 0.58 -2.2 254,836 - Pf1N1B4_5504 0.58 -2.2 254,836 - Pf1N1B4_5504 0.58 -2.7 254,893 + Pf1N1B4_5504 0.68 -0.9 254,894 - Pf1N1B4_5504 0.68 -1.0 254,894 - Pf1N1B4_5504 0.68 -0.0 255,054 + +0.9 255,055 - -1.0 255,066 + +0.8 255,067 - +1.3 255,067 - +1.2 255,131 + -0.5 255,131 + +2.3 255,131 + +0.4 255,131 + -1.0 255,131 + -0.6 255,131 + +0.6 255,131 + +0.2 255,132 - -1.5 255,132 - -1.9 255,132 - +0.2 255,132 - +1.3 255,132 - -2.2 255,132 - -0.1 255,132 - +0.8 255,242 + Pf1N1B4_5505 0.64 -1.9 255,242 + Pf1N1B4_5505 0.64 +1.8 255,242 + +3.0 255,242 + Pf1N1B4_5505 0.64 -2.5 255,242 + Pf1N1B4_5505 0.64 +1.5 255,242 + -1.2 255,242 + Pf1N1B4_5505 0.64 -2.1 255,242 + Pf1N1B4_5505 0.64 -1.6 255,242 + Pf1N1B4_5505 0.64 -2.3 255,243 - Pf1N1B4_5505 0.65 -3.1 255,243 - -1.0 255,269 + +3.3 255,269 + -1.7 255,269 + Pf1N1B4_5505 0.80 -1.8 255,269 + Pf1N1B4_5505 0.80 -1.9 255,270 - Pf1N1B4_5505 0.80 -1.3 255,270 - Pf1N1B4_5505 0.80 -0.3 255,289 + +0.3 255,592 + Pf1N1B4_5506 0.57 +0.0 255,592 + Pf1N1B4_5506 0.57 -2.5 255,592 + Pf1N1B4_5506 0.57 +0.6 255,592 + Pf1N1B4_5506 0.57 -3.3 255,592 + -1.2 255,592 + Pf1N1B4_5506 0.57 -1.6 255,593 - Pf1N1B4_5506 0.57 +0.3 255,593 - Pf1N1B4_5506 0.57 -2.7 255,593 - +2.3 255,593 - -2.2 255,689 + Pf1N1B4_5506 0.75 +0.4 255,689 + Pf1N1B4_5506 0.75 +1.8 255,689 + Pf1N1B4_5506 0.75 +2.5 255,689 + Pf1N1B4_5506 0.75 +1.1 255,691 + +2.3 255,692 - Pf1N1B4_5506 0.76 -2.3 255,740 + Pf1N1B4_5506 0.85 -1.3 255,741 - Pf1N1B4_5506 0.85 -0.3 255,741 - Pf1N1B4_5506 0.85 -2.9 255,829 + -1.5 255,829 + -1.8 255,829 + +2.3 255,830 - -0.5 255,881 + -2.5 255,881 + -1.1 255,881 + -1.0 255,882 - +0.8 255,932 + +0.7 255,933 - -2.5 255,933 - +0.2 255,933 - +0.6 255,933 - -2.9 255,933 - +0.6 255,933 - -2.8
Or see this region's nucleotide sequence