Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5498 and Pf1N1B4_5499 are separated by 367 nucleotides Pf1N1B4_5499 and Pf1N1B4_5500 are separated by 177 nucleotides
Pf1N1B4_5498 - hypothetical protein, at 247,310 to 247,522
_5498
Pf1N1B4_5499 - hypothetical protein, at 247,890 to 249,695
_5499
Pf1N1B4_5500 - hypothetical protein, at 249,873 to 250,862
_5500
Position (kb) 247
248
249
250 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 247.038 kb on + strand at 247.038 kb on + strand at 247.041 kb on - strand at 247.041 kb on - strand at 247.109 kb on + strand at 247.150 kb on - strand at 247.191 kb on + strand at 247.192 kb on - strand at 247.219 kb on + strand at 247.252 kb on + strand at 247.252 kb on + strand at 247.253 kb on - strand at 247.253 kb on - strand at 247.255 kb on - strand at 247.361 kb on + strand, within Pf1N1B4_5498 at 247.362 kb on - strand, within Pf1N1B4_5498 at 247.362 kb on - strand, within Pf1N1B4_5498 at 247.462 kb on + strand, within Pf1N1B4_5498 at 247.462 kb on + strand, within Pf1N1B4_5498 at 247.631 kb on + strand at 247.632 kb on - strand at 247.632 kb on - strand at 247.632 kb on - strand at 247.632 kb on - strand at 247.683 kb on + strand at 247.684 kb on - strand at 247.705 kb on + strand at 247.706 kb on - strand at 247.706 kb on - strand at 247.706 kb on - strand at 247.706 kb on - strand at 247.780 kb on + strand at 247.780 kb on + strand at 247.780 kb on + strand at 247.781 kb on - strand at 247.781 kb on - strand at 247.855 kb on + strand at 248.092 kb on + strand, within Pf1N1B4_5499 at 248.092 kb on + strand, within Pf1N1B4_5499 at 248.093 kb on - strand at 248.320 kb on + strand, within Pf1N1B4_5499 at 248.356 kb on + strand at 248.356 kb on + strand, within Pf1N1B4_5499 at 248.356 kb on + strand, within Pf1N1B4_5499 at 248.357 kb on - strand, within Pf1N1B4_5499 at 248.357 kb on - strand, within Pf1N1B4_5499 at 248.357 kb on - strand, within Pf1N1B4_5499 at 248.357 kb on - strand, within Pf1N1B4_5499 at 248.357 kb on - strand at 248.434 kb on + strand, within Pf1N1B4_5499 at 248.434 kb on + strand, within Pf1N1B4_5499 at 248.434 kb on + strand, within Pf1N1B4_5499 at 248.434 kb on + strand, within Pf1N1B4_5499 at 248.434 kb on + strand, within Pf1N1B4_5499 at 248.434 kb on + strand, within Pf1N1B4_5499 at 248.435 kb on - strand, within Pf1N1B4_5499 at 248.435 kb on - strand, within Pf1N1B4_5499 at 248.515 kb on + strand, within Pf1N1B4_5499 at 248.515 kb on + strand, within Pf1N1B4_5499 at 248.515 kb on + strand, within Pf1N1B4_5499 at 248.516 kb on - strand, within Pf1N1B4_5499 at 248.516 kb on - strand, within Pf1N1B4_5499 at 248.577 kb on + strand at 248.577 kb on + strand, within Pf1N1B4_5499 at 248.578 kb on - strand, within Pf1N1B4_5499 at 248.578 kb on - strand, within Pf1N1B4_5499 at 248.578 kb on - strand, within Pf1N1B4_5499 at 248.645 kb on - strand at 248.654 kb on + strand, within Pf1N1B4_5499 at 248.654 kb on + strand, within Pf1N1B4_5499 at 248.654 kb on + strand, within Pf1N1B4_5499 at 248.654 kb on + strand, within Pf1N1B4_5499 at 248.654 kb on + strand, within Pf1N1B4_5499 at 248.655 kb on - strand at 248.655 kb on - strand, within Pf1N1B4_5499 at 248.655 kb on - strand, within Pf1N1B4_5499 at 248.785 kb on + strand, within Pf1N1B4_5499 at 248.786 kb on - strand, within Pf1N1B4_5499 at 248.796 kb on + strand at 248.900 kb on + strand, within Pf1N1B4_5499 at 248.971 kb on + strand, within Pf1N1B4_5499 at 248.972 kb on - strand, within Pf1N1B4_5499 at 248.972 kb on - strand, within Pf1N1B4_5499 at 248.972 kb on - strand, within Pf1N1B4_5499 at 249.004 kb on - strand at 249.070 kb on + strand, within Pf1N1B4_5499 at 249.070 kb on + strand, within Pf1N1B4_5499 at 249.070 kb on + strand, within Pf1N1B4_5499 at 249.071 kb on - strand, within Pf1N1B4_5499 at 249.232 kb on + strand, within Pf1N1B4_5499 at 249.232 kb on + strand at 249.232 kb on + strand, within Pf1N1B4_5499 at 249.233 kb on - strand, within Pf1N1B4_5499 at 249.233 kb on - strand at 249.246 kb on - strand, within Pf1N1B4_5499 at 249.246 kb on - strand, within Pf1N1B4_5499 at 249.274 kb on + strand, within Pf1N1B4_5499 at 249.450 kb on - strand, within Pf1N1B4_5499 at 249.450 kb on - strand, within Pf1N1B4_5499 at 249.450 kb on - strand, within Pf1N1B4_5499 at 249.560 kb on + strand at 249.571 kb on + strand at 249.571 kb on + strand at 249.571 kb on + strand at 249.572 kb on - strand at 249.572 kb on - strand at 249.623 kb on + strand at 249.627 kb on + strand at 249.627 kb on + strand at 249.627 kb on + strand at 249.628 kb on - strand at 249.682 kb on + strand at 249.683 kb on - strand at 249.683 kb on - strand at 249.742 kb on - strand at 249.840 kb on - strand at 249.840 kb on - strand at 249.880 kb on + strand at 249.880 kb on + strand at 249.880 kb on + strand at 249.880 kb on + strand at 249.880 kb on + strand at 249.880 kb on + strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.882 kb on + strand at 249.883 kb on - strand at 249.883 kb on - strand at 249.883 kb on - strand at 250.144 kb on + strand, within Pf1N1B4_5500 at 250.145 kb on - strand, within Pf1N1B4_5500 at 250.224 kb on + strand, within Pf1N1B4_5500 at 250.224 kb on + strand at 250.224 kb on + strand, within Pf1N1B4_5500 at 250.224 kb on + strand at 250.224 kb on + strand, within Pf1N1B4_5500 at 250.224 kb on + strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.268 kb on - strand, within Pf1N1B4_5500 at 250.365 kb on + strand, within Pf1N1B4_5500 at 250.365 kb on + strand, within Pf1N1B4_5500 at 250.366 kb on - strand, within Pf1N1B4_5500 at 250.366 kb on - strand, within Pf1N1B4_5500 at 250.366 kb on - strand, within Pf1N1B4_5500 at 250.366 kb on - strand, within Pf1N1B4_5500 at 250.429 kb on + strand, within Pf1N1B4_5500 at 250.429 kb on + strand, within Pf1N1B4_5500 at 250.430 kb on - strand, within Pf1N1B4_5500 at 250.430 kb on - strand, within Pf1N1B4_5500 at 250.430 kb on - strand, within Pf1N1B4_5500 at 250.430 kb on - strand, within Pf1N1B4_5500 at 250.689 kb on - strand, within Pf1N1B4_5500 at 250.689 kb on - strand, within Pf1N1B4_5500
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 247,038 + -3.1 247,038 + +0.3 247,041 - -1.5 247,041 - +0.3 247,109 + +1.6 247,150 - +1.0 247,191 + -2.3 247,192 - +1.4 247,219 + -1.3 247,252 + -1.0 247,252 + -0.7 247,253 - +0.7 247,253 - -0.2 247,255 - -2.5 247,361 + Pf1N1B4_5498 0.24 -2.9 247,362 - Pf1N1B4_5498 0.24 +1.9 247,362 - Pf1N1B4_5498 0.24 -0.5 247,462 + Pf1N1B4_5498 0.71 -2.0 247,462 + Pf1N1B4_5498 0.71 -2.5 247,631 + -0.6 247,632 - +1.7 247,632 - +1.5 247,632 - -1.1 247,632 - -1.3 247,683 + +2.5 247,684 - +0.2 247,705 + +0.9 247,706 - -0.3 247,706 - -0.3 247,706 - -2.4 247,706 - +2.0 247,780 + -1.4 247,780 + +1.2 247,780 + +2.1 247,781 - +1.3 247,781 - +0.1 247,855 + +0.9 248,092 + Pf1N1B4_5499 0.11 -0.4 248,092 + Pf1N1B4_5499 0.11 -0.5 248,093 - -1.6 248,320 + Pf1N1B4_5499 0.24 +0.9 248,356 + +0.3 248,356 + Pf1N1B4_5499 0.26 -0.1 248,356 + Pf1N1B4_5499 0.26 +3.2 248,357 - Pf1N1B4_5499 0.26 -2.8 248,357 - Pf1N1B4_5499 0.26 -0.7 248,357 - Pf1N1B4_5499 0.26 +0.7 248,357 - Pf1N1B4_5499 0.26 +0.8 248,357 - -2.0 248,434 + Pf1N1B4_5499 0.30 +2.0 248,434 + Pf1N1B4_5499 0.30 +0.5 248,434 + Pf1N1B4_5499 0.30 -1.0 248,434 + Pf1N1B4_5499 0.30 -2.1 248,434 + Pf1N1B4_5499 0.30 -2.2 248,434 + Pf1N1B4_5499 0.30 -1.2 248,435 - Pf1N1B4_5499 0.30 +1.4 248,435 - Pf1N1B4_5499 0.30 -0.3 248,515 + Pf1N1B4_5499 0.35 -1.1 248,515 + Pf1N1B4_5499 0.35 +1.1 248,515 + Pf1N1B4_5499 0.35 -0.7 248,516 - Pf1N1B4_5499 0.35 +2.2 248,516 - Pf1N1B4_5499 0.35 -0.3 248,577 + +1.0 248,577 + Pf1N1B4_5499 0.38 +0.4 248,578 - Pf1N1B4_5499 0.38 -2.5 248,578 - Pf1N1B4_5499 0.38 -2.3 248,578 - Pf1N1B4_5499 0.38 +1.1 248,645 - -0.5 248,654 + Pf1N1B4_5499 0.42 -0.0 248,654 + Pf1N1B4_5499 0.42 +0.1 248,654 + Pf1N1B4_5499 0.42 -2.3 248,654 + Pf1N1B4_5499 0.42 -1.7 248,654 + Pf1N1B4_5499 0.42 +0.4 248,655 - +2.3 248,655 - Pf1N1B4_5499 0.42 -1.7 248,655 - Pf1N1B4_5499 0.42 +0.2 248,785 + Pf1N1B4_5499 0.50 -2.0 248,786 - Pf1N1B4_5499 0.50 +0.9 248,796 + +1.3 248,900 + Pf1N1B4_5499 0.56 -1.7 248,971 + Pf1N1B4_5499 0.60 +0.2 248,972 - Pf1N1B4_5499 0.60 +0.4 248,972 - Pf1N1B4_5499 0.60 +0.1 248,972 - Pf1N1B4_5499 0.60 +0.7 249,004 - -1.6 249,070 + Pf1N1B4_5499 0.65 -1.7 249,070 + Pf1N1B4_5499 0.65 -2.9 249,070 + Pf1N1B4_5499 0.65 -2.1 249,071 - Pf1N1B4_5499 0.65 -2.3 249,232 + Pf1N1B4_5499 0.74 +2.7 249,232 + -1.0 249,232 + Pf1N1B4_5499 0.74 -0.7 249,233 - Pf1N1B4_5499 0.74 +0.0 249,233 - -1.0 249,246 - Pf1N1B4_5499 0.75 +0.4 249,246 - Pf1N1B4_5499 0.75 +1.8 249,274 + Pf1N1B4_5499 0.77 -0.5 249,450 - Pf1N1B4_5499 0.86 +2.0 249,450 - Pf1N1B4_5499 0.86 -1.1 249,450 - Pf1N1B4_5499 0.86 +0.9 249,560 + +0.2 249,571 + +2.1 249,571 + +0.7 249,571 + -1.7 249,572 - -2.1 249,572 - +2.0 249,623 + -1.3 249,627 + -1.5 249,627 + -0.2 249,627 + +0.4 249,628 - -0.0 249,682 + -1.2 249,683 - -1.2 249,683 - +1.5 249,742 - +1.1 249,840 - -0.2 249,840 - -3.0 249,880 + +0.5 249,880 + +1.3 249,880 + +0.4 249,880 + -1.1 249,880 + +1.0 249,880 + -1.5 249,881 - +2.3 249,881 - +1.2 249,881 - +2.1 249,881 - +0.2 249,881 - -0.7 249,881 - -0.8 249,881 - +2.8 249,881 - -1.5 249,881 - +0.7 249,882 + -1.0 249,883 - -2.0 249,883 - -1.3 249,883 - +0.2 250,144 + Pf1N1B4_5500 0.27 +1.0 250,145 - Pf1N1B4_5500 0.27 -0.6 250,224 + Pf1N1B4_5500 0.35 +0.9 250,224 + +4.9 250,224 + Pf1N1B4_5500 0.35 -1.3 250,224 + -1.0 250,224 + Pf1N1B4_5500 0.35 +1.3 250,224 + Pf1N1B4_5500 0.35 +1.3 250,225 - Pf1N1B4_5500 0.36 -3.4 250,225 - Pf1N1B4_5500 0.36 +0.3 250,225 - Pf1N1B4_5500 0.36 -0.0 250,225 - Pf1N1B4_5500 0.36 -1.4 250,225 - Pf1N1B4_5500 0.36 -1.9 250,225 - Pf1N1B4_5500 0.36 +1.3 250,225 - Pf1N1B4_5500 0.36 +0.8 250,268 - Pf1N1B4_5500 0.40 -1.6 250,365 + Pf1N1B4_5500 0.50 -1.0 250,365 + Pf1N1B4_5500 0.50 +0.2 250,366 - Pf1N1B4_5500 0.50 +2.0 250,366 - Pf1N1B4_5500 0.50 -0.3 250,366 - Pf1N1B4_5500 0.50 -2.5 250,366 - Pf1N1B4_5500 0.50 +0.7 250,429 + Pf1N1B4_5500 0.56 +1.3 250,429 + Pf1N1B4_5500 0.56 +0.8 250,430 - Pf1N1B4_5500 0.56 -1.5 250,430 - Pf1N1B4_5500 0.56 -1.5 250,430 - Pf1N1B4_5500 0.56 -0.3 250,430 - Pf1N1B4_5500 0.56 +0.7 250,689 - Pf1N1B4_5500 0.82 +1.2 250,689 - Pf1N1B4_5500 0.82 -0.6
Or see this region's nucleotide sequence