Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5423 and Pf1N1B4_5424 are separated by 5 nucleotides Pf1N1B4_5424 and Pf1N1B4_5425 are separated by 543 nucleotides Pf1N1B4_5425 and Pf1N1B4_5426 are separated by 461 nucleotides Pf1N1B4_5426 and Pf1N1B4_5427 are separated by 130 nucleotides
Pf1N1B4_5423 - RecD-like DNA helicase YrrC, at 162,581 to 164,764
_5423
Pf1N1B4_5424 - hypothetical protein, at 164,770 to 164,952
_5424
Pf1N1B4_5425 - DNA-binding protein HU-beta, at 165,496 to 165,771
_5425
Pf1N1B4_5426 - hypothetical protein, at 166,233 to 166,580
_5426
Pf1N1B4_5427 - C4-type zinc finger protein, DksA/TraR family, at 166,711 to 167,184
_5427
Position (kb) 165
166 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 164.499 kb on + strand, within Pf1N1B4_5423 at 164.500 kb on - strand, within Pf1N1B4_5423 at 164.500 kb on - strand, within Pf1N1B4_5423 at 164.533 kb on + strand, within Pf1N1B4_5423 at 164.533 kb on + strand, within Pf1N1B4_5423 at 164.740 kb on + strand at 164.839 kb on - strand, within Pf1N1B4_5424 at 164.839 kb on - strand at 164.895 kb on - strand, within Pf1N1B4_5424 at 164.895 kb on - strand, within Pf1N1B4_5424 at 165.160 kb on + strand at 165.244 kb on + strand at 165.244 kb on + strand at 165.244 kb on + strand at 165.244 kb on + strand at 165.244 kb on + strand at 165.269 kb on + strand at 165.276 kb on + strand at 165.276 kb on + strand at 165.276 kb on + strand at 165.276 kb on + strand at 165.279 kb on + strand at 165.279 kb on + strand at 165.280 kb on - strand at 165.337 kb on + strand at 165.419 kb on + strand at 165.419 kb on + strand at 165.535 kb on - strand, within Pf1N1B4_5425 at 165.640 kb on + strand, within Pf1N1B4_5425 at 165.641 kb on - strand, within Pf1N1B4_5425 at 165.641 kb on - strand, within Pf1N1B4_5425 at 165.724 kb on + strand, within Pf1N1B4_5425 at 165.725 kb on - strand, within Pf1N1B4_5425 at 165.828 kb on - strand at 165.848 kb on + strand at 165.849 kb on - strand at 165.903 kb on - strand at 165.949 kb on + strand at 165.950 kb on - strand at 165.950 kb on - strand at 166.031 kb on - strand at 166.031 kb on - strand at 166.031 kb on - strand at 166.128 kb on - strand at 166.211 kb on - strand at 166.222 kb on + strand at 166.222 kb on + strand at 166.223 kb on - strand at 166.274 kb on + strand, within Pf1N1B4_5426 at 166.274 kb on + strand, within Pf1N1B4_5426 at 166.274 kb on + strand, within Pf1N1B4_5426 at 166.275 kb on - strand, within Pf1N1B4_5426 at 166.275 kb on - strand, within Pf1N1B4_5426 at 166.287 kb on + strand, within Pf1N1B4_5426 at 166.287 kb on + strand, within Pf1N1B4_5426 at 166.287 kb on + strand, within Pf1N1B4_5426 at 166.287 kb on + strand, within Pf1N1B4_5426 at 166.287 kb on + strand, within Pf1N1B4_5426 at 166.288 kb on - strand, within Pf1N1B4_5426 at 166.288 kb on - strand, within Pf1N1B4_5426 at 166.288 kb on - strand, within Pf1N1B4_5426 at 166.347 kb on - strand, within Pf1N1B4_5426 at 166.371 kb on + strand, within Pf1N1B4_5426 at 166.372 kb on - strand, within Pf1N1B4_5426 at 166.439 kb on - strand, within Pf1N1B4_5426 at 166.532 kb on - strand, within Pf1N1B4_5426 at 166.603 kb on + strand at 166.603 kb on + strand at 166.603 kb on + strand at 166.603 kb on + strand at 166.603 kb on + strand at 166.604 kb on - strand at 166.604 kb on - strand at 166.604 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 164,499 + Pf1N1B4_5423 0.88 +0.6 164,500 - Pf1N1B4_5423 0.88 +1.9 164,500 - Pf1N1B4_5423 0.88 +0.2 164,533 + Pf1N1B4_5423 0.89 +1.0 164,533 + Pf1N1B4_5423 0.89 +0.7 164,740 + +1.6 164,839 - Pf1N1B4_5424 0.38 +1.4 164,839 - -0.7 164,895 - Pf1N1B4_5424 0.68 +1.0 164,895 - Pf1N1B4_5424 0.68 -1.4 165,160 + -1.5 165,244 + -3.2 165,244 + +1.2 165,244 + -1.4 165,244 + -2.2 165,244 + +1.2 165,269 + -0.9 165,276 + +1.2 165,276 + -1.3 165,276 + -1.3 165,276 + -1.7 165,279 + -2.8 165,279 + +1.7 165,280 - +2.3 165,337 + +1.0 165,419 + -0.7 165,419 + -1.7 165,535 - Pf1N1B4_5425 0.14 -0.4 165,640 + Pf1N1B4_5425 0.52 -2.3 165,641 - Pf1N1B4_5425 0.53 -1.7 165,641 - Pf1N1B4_5425 0.53 -1.5 165,724 + Pf1N1B4_5425 0.83 -1.3 165,725 - Pf1N1B4_5425 0.83 -1.8 165,828 - +0.2 165,848 + -1.0 165,849 - +1.9 165,903 - -0.3 165,949 + +0.2 165,950 - +0.7 165,950 - -0.9 166,031 - -1.5 166,031 - -0.1 166,031 - -2.7 166,128 - +1.5 166,211 - +0.8 166,222 + +1.5 166,222 + +0.3 166,223 - +0.7 166,274 + Pf1N1B4_5426 0.12 +0.8 166,274 + Pf1N1B4_5426 0.12 +1.7 166,274 + Pf1N1B4_5426 0.12 +0.6 166,275 - Pf1N1B4_5426 0.12 -0.2 166,275 - Pf1N1B4_5426 0.12 -1.7 166,287 + Pf1N1B4_5426 0.16 -1.6 166,287 + Pf1N1B4_5426 0.16 +0.5 166,287 + Pf1N1B4_5426 0.16 +0.4 166,287 + Pf1N1B4_5426 0.16 -1.9 166,287 + Pf1N1B4_5426 0.16 +1.1 166,288 - Pf1N1B4_5426 0.16 +2.3 166,288 - Pf1N1B4_5426 0.16 -1.0 166,288 - Pf1N1B4_5426 0.16 -1.9 166,347 - Pf1N1B4_5426 0.33 +0.7 166,371 + Pf1N1B4_5426 0.40 +0.8 166,372 - Pf1N1B4_5426 0.40 -1.4 166,439 - Pf1N1B4_5426 0.59 +1.2 166,532 - Pf1N1B4_5426 0.86 +1.9 166,603 + +2.0 166,603 + -1.3 166,603 + +2.6 166,603 + -2.9 166,603 + -1.4 166,604 - -1.6 166,604 - -1.5 166,604 - +1.8
Or see this region's nucleotide sequence