Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5329 and Pf1N1B4_5330 are separated by 25 nucleotides Pf1N1B4_5330 and Pf1N1B4_5331 are separated by 356 nucleotides Pf1N1B4_5331 and Pf1N1B4_5332 are separated by 2 nucleotides Pf1N1B4_5332 and Pf1N1B4_5333 are separated by 11 nucleotides Pf1N1B4_5333 and Pf1N1B4_5334 are separated by 131 nucleotides
Pf1N1B4_5329 - hypothetical protein, at 77,354 to 77,896
_5329
Pf1N1B4_5330 - hypothetical protein, at 77,922 to 78,233
_5330
Pf1N1B4_5331 - hypothetical protein, at 78,590 to 78,760
_5331
Pf1N1B4_5332 - hypothetical protein, at 78,763 to 78,957
_5332
Pf1N1B4_5333 - hypothetical protein, at 78,969 to 79,595
_5333
Pf1N1B4_5334 - hypothetical protein, at 79,727 to 79,930
_5334
Position (kb) 78
79 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 77.686 kb on + strand, within Pf1N1B4_5329 at 77.752 kb on - strand, within Pf1N1B4_5329 at 77.781 kb on + strand, within Pf1N1B4_5329 at 77.781 kb on + strand at 77.782 kb on - strand, within Pf1N1B4_5329 at 77.847 kb on + strand at 77.923 kb on + strand at 77.923 kb on + strand at 77.980 kb on + strand, within Pf1N1B4_5330 at 78.022 kb on - strand, within Pf1N1B4_5330 at 78.152 kb on + strand at 78.152 kb on + strand, within Pf1N1B4_5330 at 78.153 kb on - strand at 78.313 kb on + strand at 78.313 kb on + strand at 78.313 kb on + strand at 78.313 kb on + strand at 78.314 kb on - strand at 78.336 kb on + strand at 78.336 kb on + strand at 78.336 kb on + strand at 78.336 kb on + strand at 78.351 kb on + strand at 78.351 kb on + strand at 78.351 kb on + strand at 78.352 kb on - strand at 78.352 kb on - strand at 78.392 kb on + strand at 78.392 kb on + strand at 78.425 kb on + strand at 78.425 kb on + strand at 78.426 kb on - strand at 78.426 kb on - strand at 78.474 kb on + strand at 78.474 kb on + strand at 78.475 kb on - strand at 78.475 kb on - strand at 78.475 kb on - strand at 78.475 kb on - strand at 78.475 kb on - strand at 78.597 kb on - strand at 78.597 kb on - strand at 78.597 kb on - strand at 78.729 kb on + strand, within Pf1N1B4_5331 at 78.730 kb on - strand, within Pf1N1B4_5331 at 78.730 kb on - strand, within Pf1N1B4_5331 at 78.730 kb on - strand, within Pf1N1B4_5331 at 78.739 kb on - strand, within Pf1N1B4_5331 at 78.971 kb on - strand at 79.045 kb on + strand, within Pf1N1B4_5333 at 79.045 kb on + strand, within Pf1N1B4_5333 at 79.046 kb on - strand, within Pf1N1B4_5333 at 79.046 kb on - strand, within Pf1N1B4_5333 at 79.046 kb on - strand, within Pf1N1B4_5333 at 79.106 kb on + strand, within Pf1N1B4_5333 at 79.268 kb on + strand, within Pf1N1B4_5333 at 79.268 kb on + strand, within Pf1N1B4_5333 at 79.269 kb on - strand, within Pf1N1B4_5333 at 79.269 kb on - strand, within Pf1N1B4_5333 at 79.393 kb on - strand, within Pf1N1B4_5333 at 79.393 kb on - strand, within Pf1N1B4_5333 at 79.446 kb on - strand, within Pf1N1B4_5333 at 79.501 kb on + strand, within Pf1N1B4_5333 at 79.501 kb on + strand, within Pf1N1B4_5333 at 79.502 kb on - strand, within Pf1N1B4_5333 at 79.502 kb on - strand, within Pf1N1B4_5333 at 79.576 kb on + strand at 79.576 kb on + strand at 79.576 kb on + strand at 79.576 kb on + strand at 79.577 kb on - strand at 79.577 kb on - strand at 79.577 kb on - strand at 79.577 kb on - strand at 79.577 kb on - strand at 79.580 kb on + strand at 79.580 kb on + strand at 79.726 kb on - strand at 79.728 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 77,686 + Pf1N1B4_5329 0.61 -2.3 77,752 - Pf1N1B4_5329 0.73 -0.9 77,781 + Pf1N1B4_5329 0.79 -2.3 77,781 + +1.0 77,782 - Pf1N1B4_5329 0.79 +0.5 77,847 + -2.7 77,923 + +0.2 77,923 + -1.9 77,980 + Pf1N1B4_5330 0.19 +0.6 78,022 - Pf1N1B4_5330 0.32 -1.2 78,152 + -1.5 78,152 + Pf1N1B4_5330 0.74 -0.9 78,153 - -1.4 78,313 + -0.4 78,313 + +0.6 78,313 + -0.3 78,313 + -1.6 78,314 - +1.6 78,336 + -0.7 78,336 + +1.0 78,336 + +0.3 78,336 + +0.8 78,351 + -1.4 78,351 + -0.9 78,351 + -0.3 78,352 - -0.3 78,352 - +1.2 78,392 + -1.6 78,392 + -2.5 78,425 + +1.5 78,425 + +3.4 78,426 - -0.4 78,426 - +0.4 78,474 + +0.7 78,474 + -1.2 78,475 - -0.1 78,475 - +1.5 78,475 - -0.8 78,475 - +1.6 78,475 - -2.6 78,597 - +1.0 78,597 - -2.4 78,597 - +1.3 78,729 + Pf1N1B4_5331 0.81 -0.7 78,730 - Pf1N1B4_5331 0.82 -2.7 78,730 - Pf1N1B4_5331 0.82 +0.4 78,730 - Pf1N1B4_5331 0.82 -1.9 78,739 - Pf1N1B4_5331 0.87 -1.0 78,971 - +1.9 79,045 + Pf1N1B4_5333 0.12 -2.5 79,045 + Pf1N1B4_5333 0.12 -1.2 79,046 - Pf1N1B4_5333 0.12 -1.8 79,046 - Pf1N1B4_5333 0.12 +1.9 79,046 - Pf1N1B4_5333 0.12 +1.7 79,106 + Pf1N1B4_5333 0.22 +1.6 79,268 + Pf1N1B4_5333 0.48 -1.4 79,268 + Pf1N1B4_5333 0.48 +1.0 79,269 - Pf1N1B4_5333 0.48 +0.1 79,269 - Pf1N1B4_5333 0.48 +1.7 79,393 - Pf1N1B4_5333 0.68 -0.4 79,393 - Pf1N1B4_5333 0.68 +1.3 79,446 - Pf1N1B4_5333 0.76 +0.7 79,501 + Pf1N1B4_5333 0.85 -2.5 79,501 + Pf1N1B4_5333 0.85 -0.9 79,502 - Pf1N1B4_5333 0.85 -2.8 79,502 - Pf1N1B4_5333 0.85 -1.4 79,576 + -0.1 79,576 + +2.5 79,576 + -2.2 79,576 + -1.7 79,577 - +1.8 79,577 - +1.2 79,577 - -1.3 79,577 - +0.6 79,577 - +3.0 79,580 + +1.3 79,580 + -2.2 79,726 - -1.8 79,728 + +1.8
Or see this region's nucleotide sequence