Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4594

Experiment: nitrogen source nitrite 0.1mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4593 and Pf1N1B4_4594 overlap by 4 nucleotidesPf1N1B4_4594 and Pf1N1B4_4595 are separated by 282 nucleotides Pf1N1B4_4593 - ABC transporter, periplasmic substrate-binding protein, at 5,064,001 to 5,065,842 _4593 Pf1N1B4_4594 - Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1), at 5,065,839 to 5,067,671 _4594 Pf1N1B4_4595 - Membrane-bound lytic murein transglycosylase D precursor (EC 3.2.1.-), at 5,067,954 to 5,069,408 _4595 Position (kb) 5065 5066 5067 5068Strain fitness (log2 ratio) -2 -1 0 1 2at 5064.983 kb on + strand, within Pf1N1B4_4593at 5064.984 kb on - strand, within Pf1N1B4_4593at 5064.984 kb on - strand, within Pf1N1B4_4593at 5064.984 kb on - strand, within Pf1N1B4_4593at 5065.448 kb on - strand, within Pf1N1B4_4593at 5065.487 kb on + strand, within Pf1N1B4_4593at 5065.488 kb on - strand, within Pf1N1B4_4593at 5065.589 kb on + strand, within Pf1N1B4_4593at 5065.703 kb on - strandat 5065.942 kb on + strandat 5065.942 kb on + strandat 5065.942 kb on + strandat 5065.969 kb on + strandat 5065.970 kb on - strandat 5065.970 kb on - strandat 5066.218 kb on + strand, within Pf1N1B4_4594at 5066.570 kb on - strandat 5066.588 kb on - strand, within Pf1N1B4_4594at 5066.633 kb on + strand, within Pf1N1B4_4594at 5066.894 kb on - strand, within Pf1N1B4_4594at 5067.212 kb on - strand, within Pf1N1B4_4594at 5067.223 kb on - strand, within Pf1N1B4_4594at 5067.763 kb on + strandat 5068.067 kb on - strandat 5068.067 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM
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5,064,983 + Pf1N1B4_4593 0.53 -1.6
5,064,984 - Pf1N1B4_4593 0.53 -2.0
5,064,984 - Pf1N1B4_4593 0.53 -2.0
5,064,984 - Pf1N1B4_4593 0.53 -2.6
5,065,448 - Pf1N1B4_4593 0.79 -1.1
5,065,487 + Pf1N1B4_4593 0.81 +0.4
5,065,488 - Pf1N1B4_4593 0.81 +0.8
5,065,589 + Pf1N1B4_4593 0.86 -0.2
5,065,703 - +0.4
5,065,942 + -1.0
5,065,942 + -2.1
5,065,942 + +1.9
5,065,969 + -0.4
5,065,970 - +0.4
5,065,970 - +0.7
5,066,218 + Pf1N1B4_4594 0.21 -2.1
5,066,570 - +1.2
5,066,588 - Pf1N1B4_4594 0.41 +0.8
5,066,633 + Pf1N1B4_4594 0.43 +1.8
5,066,894 - Pf1N1B4_4594 0.58 -1.3
5,067,212 - Pf1N1B4_4594 0.75 +1.5
5,067,223 - Pf1N1B4_4594 0.76 -0.8
5,067,763 + +0.1
5,068,067 - -1.4
5,068,067 - -1.2

Or see this region's nucleotide sequence