Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3527

Experiment: nitrogen source nitrite 0.1mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3525 and Pf1N1B4_3526 are separated by 50 nucleotidesPf1N1B4_3526 and Pf1N1B4_3527 are separated by 8 nucleotidesPf1N1B4_3527 and Pf1N1B4_3528 are separated by 68 nucleotides Pf1N1B4_3525 - hydrolase, alpha/beta fold family, at 3,931,039 to 3,931,893 _3525 Pf1N1B4_3526 - Glutathione S-transferase (EC 2.5.1.18), at 3,931,944 to 3,932,603 _3526 Pf1N1B4_3527 - Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit, at 3,932,612 to 3,933,418 _3527 Pf1N1B4_3528 - Putative membrane protein, at 3,933,487 to 3,934,542 _3528 Position (kb) 3932 3933 3934Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3931.629 kb on + strand, within Pf1N1B4_3525at 3931.629 kb on + strand, within Pf1N1B4_3525at 3931.630 kb on - strandat 3931.948 kb on + strandat 3931.948 kb on + strandat 3931.948 kb on + strandat 3931.949 kb on - strandat 3931.949 kb on - strandat 3931.956 kb on + strandat 3931.956 kb on + strandat 3932.164 kb on - strand, within Pf1N1B4_3526at 3932.352 kb on + strand, within Pf1N1B4_3526at 3932.352 kb on + strand, within Pf1N1B4_3526at 3932.352 kb on + strand, within Pf1N1B4_3526at 3932.352 kb on + strand, within Pf1N1B4_3526at 3932.352 kb on + strand, within Pf1N1B4_3526at 3932.352 kb on + strand, within Pf1N1B4_3526at 3932.352 kb on + strand, within Pf1N1B4_3526at 3932.403 kb on + strand, within Pf1N1B4_3526at 3932.427 kb on + strand, within Pf1N1B4_3526at 3932.608 kb on - strandat 3932.672 kb on - strandat 3932.849 kb on + strandat 3932.850 kb on - strand, within Pf1N1B4_3527at 3932.850 kb on - strand, within Pf1N1B4_3527at 3932.850 kb on - strand, within Pf1N1B4_3527at 3932.932 kb on - strand, within Pf1N1B4_3527at 3932.932 kb on - strand, within Pf1N1B4_3527at 3933.005 kb on + strand, within Pf1N1B4_3527at 3933.030 kb on - strand, within Pf1N1B4_3527at 3933.030 kb on - strand, within Pf1N1B4_3527at 3933.034 kb on - strand, within Pf1N1B4_3527at 3933.080 kb on + strand, within Pf1N1B4_3527at 3933.081 kb on - strand, within Pf1N1B4_3527at 3933.110 kb on + strand, within Pf1N1B4_3527at 3933.351 kb on + strandat 3933.454 kb on - strandat 3933.484 kb on + strandat 3933.484 kb on + strandat 3933.484 kb on + strandat 3933.484 kb on + strandat 3933.484 kb on + strandat 3933.485 kb on - strandat 3933.485 kb on - strandat 3933.773 kb on + strand, within Pf1N1B4_3528at 3933.774 kb on - strand, within Pf1N1B4_3528at 3933.774 kb on - strand, within Pf1N1B4_3528at 3933.774 kb on - strand, within Pf1N1B4_3528at 3933.978 kb on + strand, within Pf1N1B4_3528at 3934.075 kb on + strand, within Pf1N1B4_3528at 3934.075 kb on + strand, within Pf1N1B4_3528at 3934.075 kb on + strand, within Pf1N1B4_3528at 3934.075 kb on + strand, within Pf1N1B4_3528at 3934.075 kb on + strand, within Pf1N1B4_3528at 3934.076 kb on - strandat 3934.352 kb on + strand, within Pf1N1B4_3528at 3934.352 kb on + strand, within Pf1N1B4_3528at 3934.352 kb on + strandat 3934.352 kb on + strand, within Pf1N1B4_3528at 3934.352 kb on + strand, within Pf1N1B4_3528at 3934.352 kb on + strand, within Pf1N1B4_3528at 3934.352 kb on + strand, within Pf1N1B4_3528at 3934.353 kb on - strandat 3934.356 kb on + strand, within Pf1N1B4_3528at 3934.356 kb on + strandat 3934.356 kb on + strand, within Pf1N1B4_3528at 3934.356 kb on + strand, within Pf1N1B4_3528at 3934.357 kb on - strand, within Pf1N1B4_3528

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM
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3,931,629 + Pf1N1B4_3525 0.69 +1.7
3,931,629 + Pf1N1B4_3525 0.69 +1.8
3,931,630 - -0.3
3,931,948 + -0.4
3,931,948 + -0.1
3,931,948 + +0.0
3,931,949 - -0.3
3,931,949 - -0.3
3,931,956 + +0.6
3,931,956 + +0.3
3,932,164 - Pf1N1B4_3526 0.33 -0.6
3,932,352 + Pf1N1B4_3526 0.62 +0.2
3,932,352 + Pf1N1B4_3526 0.62 +2.6
3,932,352 + Pf1N1B4_3526 0.62 -2.0
3,932,352 + Pf1N1B4_3526 0.62 +1.4
3,932,352 + Pf1N1B4_3526 0.62 +0.3
3,932,352 + Pf1N1B4_3526 0.62 -0.1
3,932,352 + Pf1N1B4_3526 0.62 -0.3
3,932,403 + Pf1N1B4_3526 0.70 +0.2
3,932,427 + Pf1N1B4_3526 0.73 +1.1
3,932,608 - -2.0
3,932,672 - +1.3
3,932,849 + -1.3
3,932,850 - Pf1N1B4_3527 0.29 +0.9
3,932,850 - Pf1N1B4_3527 0.29 -0.7
3,932,850 - Pf1N1B4_3527 0.29 +0.6
3,932,932 - Pf1N1B4_3527 0.40 +0.5
3,932,932 - Pf1N1B4_3527 0.40 -0.7
3,933,005 + Pf1N1B4_3527 0.49 +1.0
3,933,030 - Pf1N1B4_3527 0.52 -1.8
3,933,030 - Pf1N1B4_3527 0.52 +0.3
3,933,034 - Pf1N1B4_3527 0.52 +0.3
3,933,080 + Pf1N1B4_3527 0.58 +1.3
3,933,081 - Pf1N1B4_3527 0.58 +0.2
3,933,110 + Pf1N1B4_3527 0.62 +0.4
3,933,351 + -1.5
3,933,454 - -0.8
3,933,484 + +2.7
3,933,484 + -2.1
3,933,484 + -1.7
3,933,484 + -0.6
3,933,484 + +0.6
3,933,485 - -2.6
3,933,485 - -3.2
3,933,773 + Pf1N1B4_3528 0.27 +1.2
3,933,774 - Pf1N1B4_3528 0.27 -0.2
3,933,774 - Pf1N1B4_3528 0.27 +1.8
3,933,774 - Pf1N1B4_3528 0.27 -0.4
3,933,978 + Pf1N1B4_3528 0.46 -2.1
3,934,075 + Pf1N1B4_3528 0.56 -1.8
3,934,075 + Pf1N1B4_3528 0.56 -0.4
3,934,075 + Pf1N1B4_3528 0.56 -2.3
3,934,075 + Pf1N1B4_3528 0.56 -1.4
3,934,075 + Pf1N1B4_3528 0.56 -1.9
3,934,076 - -1.6
3,934,352 + Pf1N1B4_3528 0.82 +0.7
3,934,352 + Pf1N1B4_3528 0.82 -2.5
3,934,352 + +0.9
3,934,352 + Pf1N1B4_3528 0.82 +2.6
3,934,352 + Pf1N1B4_3528 0.82 +0.8
3,934,352 + Pf1N1B4_3528 0.82 +2.6
3,934,352 + Pf1N1B4_3528 0.82 +1.0
3,934,353 - +1.5
3,934,356 + Pf1N1B4_3528 0.82 -2.5
3,934,356 + -1.8
3,934,356 + Pf1N1B4_3528 0.82 -0.4
3,934,356 + Pf1N1B4_3528 0.82 +2.0
3,934,357 - Pf1N1B4_3528 0.82 +1.9

Or see this region's nucleotide sequence