Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3525

Experiment: nitrogen source nitrite 0.1mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3523 and Pf1N1B4_3524 are separated by 110 nucleotidesPf1N1B4_3524 and Pf1N1B4_3525 overlap by 4 nucleotidesPf1N1B4_3525 and Pf1N1B4_3526 are separated by 50 nucleotidesPf1N1B4_3526 and Pf1N1B4_3527 are separated by 8 nucleotides Pf1N1B4_3523 - hypothetical protein, at 3,929,988 to 3,930,125 _3523 Pf1N1B4_3524 - FIG00955363: hypothetical protein, at 3,930,236 to 3,931,042 _3524 Pf1N1B4_3525 - hydrolase, alpha/beta fold family, at 3,931,039 to 3,931,893 _3525 Pf1N1B4_3526 - Glutathione S-transferase (EC 2.5.1.18), at 3,931,944 to 3,932,603 _3526 Pf1N1B4_3527 - Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit, at 3,932,612 to 3,933,418 _3527 Position (kb) 3931 3932Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3930.133 kb on + strandat 3930.181 kb on + strandat 3930.235 kb on + strandat 3930.235 kb on + strandat 3930.235 kb on + strandat 3930.235 kb on + strandat 3930.236 kb on - strandat 3930.236 kb on - strandat 3930.236 kb on - strandat 3930.236 kb on - strandat 3930.249 kb on + strandat 3930.250 kb on - strandat 3930.250 kb on - strandat 3930.250 kb on - strandat 3930.288 kb on - strandat 3930.288 kb on - strandat 3930.321 kb on - strand, within Pf1N1B4_3524at 3930.674 kb on - strandat 3930.674 kb on - strand, within Pf1N1B4_3524at 3930.716 kb on + strand, within Pf1N1B4_3524at 3930.716 kb on + strand, within Pf1N1B4_3524at 3930.791 kb on + strand, within Pf1N1B4_3524at 3930.792 kb on - strand, within Pf1N1B4_3524at 3930.792 kb on - strand, within Pf1N1B4_3524at 3930.792 kb on - strand, within Pf1N1B4_3524at 3930.949 kb on + strand, within Pf1N1B4_3524at 3930.949 kb on + strand, within Pf1N1B4_3524at 3930.949 kb on + strand, within Pf1N1B4_3524at 3931.299 kb on - strand, within Pf1N1B4_3525at 3931.431 kb on + strand, within Pf1N1B4_3525at 3931.516 kb on + strandat 3931.516 kb on + strand, within Pf1N1B4_3525at 3931.516 kb on + strand, within Pf1N1B4_3525at 3931.629 kb on + strand, within Pf1N1B4_3525at 3931.629 kb on + strand, within Pf1N1B4_3525at 3931.630 kb on - strandat 3931.948 kb on + strandat 3931.948 kb on + strandat 3931.948 kb on + strandat 3931.949 kb on - strandat 3931.949 kb on - strandat 3931.956 kb on + strandat 3931.956 kb on + strandat 3932.164 kb on - strand, within Pf1N1B4_3526at 3932.352 kb on + strand, within Pf1N1B4_3526at 3932.352 kb on + strand, within Pf1N1B4_3526at 3932.352 kb on + strand, within Pf1N1B4_3526at 3932.352 kb on + strand, within Pf1N1B4_3526at 3932.352 kb on + strand, within Pf1N1B4_3526at 3932.352 kb on + strand, within Pf1N1B4_3526at 3932.352 kb on + strand, within Pf1N1B4_3526at 3932.403 kb on + strand, within Pf1N1B4_3526at 3932.427 kb on + strand, within Pf1N1B4_3526at 3932.608 kb on - strandat 3932.672 kb on - strandat 3932.849 kb on + strandat 3932.850 kb on - strand, within Pf1N1B4_3527at 3932.850 kb on - strand, within Pf1N1B4_3527at 3932.850 kb on - strand, within Pf1N1B4_3527

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM
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3,930,133 + -2.9
3,930,181 + -0.3
3,930,235 + -2.2
3,930,235 + -1.9
3,930,235 + +1.2
3,930,235 + -1.8
3,930,236 - -0.6
3,930,236 - -0.3
3,930,236 - -1.9
3,930,236 - -2.3
3,930,249 + -1.8
3,930,250 - +0.8
3,930,250 - -2.5
3,930,250 - -1.5
3,930,288 - -2.3
3,930,288 - +1.4
3,930,321 - Pf1N1B4_3524 0.11 -1.1
3,930,674 - +2.2
3,930,674 - Pf1N1B4_3524 0.54 -1.6
3,930,716 + Pf1N1B4_3524 0.59 -1.0
3,930,716 + Pf1N1B4_3524 0.59 -2.0
3,930,791 + Pf1N1B4_3524 0.69 -3.1
3,930,792 - Pf1N1B4_3524 0.69 -1.2
3,930,792 - Pf1N1B4_3524 0.69 +2.0
3,930,792 - Pf1N1B4_3524 0.69 -1.3
3,930,949 + Pf1N1B4_3524 0.88 +0.6
3,930,949 + Pf1N1B4_3524 0.88 -1.7
3,930,949 + Pf1N1B4_3524 0.88 -0.1
3,931,299 - Pf1N1B4_3525 0.30 -1.4
3,931,431 + Pf1N1B4_3525 0.46 +0.8
3,931,516 + -1.7
3,931,516 + Pf1N1B4_3525 0.56 -1.0
3,931,516 + Pf1N1B4_3525 0.56 -1.9
3,931,629 + Pf1N1B4_3525 0.69 +1.7
3,931,629 + Pf1N1B4_3525 0.69 +1.8
3,931,630 - -0.3
3,931,948 + -0.4
3,931,948 + -0.1
3,931,948 + +0.0
3,931,949 - -0.3
3,931,949 - -0.3
3,931,956 + +0.6
3,931,956 + +0.3
3,932,164 - Pf1N1B4_3526 0.33 -0.6
3,932,352 + Pf1N1B4_3526 0.62 +0.2
3,932,352 + Pf1N1B4_3526 0.62 +2.6
3,932,352 + Pf1N1B4_3526 0.62 -2.0
3,932,352 + Pf1N1B4_3526 0.62 +1.4
3,932,352 + Pf1N1B4_3526 0.62 +0.3
3,932,352 + Pf1N1B4_3526 0.62 -0.1
3,932,352 + Pf1N1B4_3526 0.62 -0.3
3,932,403 + Pf1N1B4_3526 0.70 +0.2
3,932,427 + Pf1N1B4_3526 0.73 +1.1
3,932,608 - -2.0
3,932,672 - +1.3
3,932,849 + -1.3
3,932,850 - Pf1N1B4_3527 0.29 +0.9
3,932,850 - Pf1N1B4_3527 0.29 -0.7
3,932,850 - Pf1N1B4_3527 0.29 +0.6

Or see this region's nucleotide sequence