Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2837 and Pf1N1B4_2838 are separated by 9 nucleotides Pf1N1B4_2838 and Pf1N1B4_2839 overlap by 4 nucleotides Pf1N1B4_2839 and Pf1N1B4_2840 are separated by 8 nucleotides Pf1N1B4_2840 and Pf1N1B4_2841 are separated by 1 nucleotides
Pf1N1B4_2837 - FIG00956650: hypothetical protein, at 3,183,355 to 3,183,570
_2837
Pf1N1B4_2838 - FIG003276: zinc-binding protein, at 3,183,580 to 3,183,780
_2838
Pf1N1B4_2839 - Dephospho-CoA kinase (EC 2.7.1.24), at 3,183,777 to 3,184,388
_2839
Pf1N1B4_2840 - Leader peptidase (Prepilin peptidase) (EC 3.4.23.43) / N-methyltransferase (EC 2.1.1.-), at 3,184,397 to 3,185,269
_2840
Pf1N1B4_2841 - Type IV fimbrial assembly protein PilC, at 3,185,271 to 3,186,488
_2841
Position (kb)
3184
3185
3186 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3183.489 kb on + strand, within Pf1N1B4_2837 at 3183.489 kb on + strand, within Pf1N1B4_2837 at 3183.489 kb on + strand, within Pf1N1B4_2837 at 3183.489 kb on + strand, within Pf1N1B4_2837 at 3183.489 kb on + strand, within Pf1N1B4_2837 at 3183.490 kb on - strand, within Pf1N1B4_2837 at 3183.490 kb on - strand, within Pf1N1B4_2837 at 3183.491 kb on + strand, within Pf1N1B4_2837 at 3183.491 kb on + strand, within Pf1N1B4_2837 at 3183.491 kb on + strand, within Pf1N1B4_2837 at 3183.491 kb on + strand, within Pf1N1B4_2837 at 3183.491 kb on + strand, within Pf1N1B4_2837 at 3183.491 kb on + strand, within Pf1N1B4_2837 at 3183.491 kb on + strand, within Pf1N1B4_2837 at 3183.491 kb on + strand, within Pf1N1B4_2837 at 3183.491 kb on + strand, within Pf1N1B4_2837 at 3183.491 kb on + strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.492 kb on - strand, within Pf1N1B4_2837 at 3183.550 kb on + strand at 3183.551 kb on - strand at 3183.558 kb on + strand at 3183.800 kb on + strand at 3183.800 kb on + strand at 3183.800 kb on + strand at 3183.800 kb on + strand at 3183.800 kb on + strand at 3183.801 kb on - strand at 3183.801 kb on - strand at 3183.801 kb on - strand at 3183.801 kb on - strand at 3183.801 kb on - strand at 3183.801 kb on - strand at 3183.801 kb on - strand at 3183.801 kb on - strand at 3183.801 kb on - strand at 3183.801 kb on - strand at 3184.395 kb on - strand at 3184.423 kb on - strand at 3184.423 kb on - strand at 3184.423 kb on - strand at 3184.423 kb on - strand at 3184.423 kb on - strand at 3184.434 kb on - strand at 3184.434 kb on - strand at 3184.480 kb on - strand at 3184.673 kb on + strand, within Pf1N1B4_2840 at 3185.001 kb on + strand, within Pf1N1B4_2840 at 3185.001 kb on + strand, within Pf1N1B4_2840 at 3185.002 kb on - strand at 3185.002 kb on - strand, within Pf1N1B4_2840 at 3185.002 kb on - strand, within Pf1N1B4_2840 at 3185.002 kb on - strand, within Pf1N1B4_2840 at 3185.002 kb on - strand, within Pf1N1B4_2840 at 3185.073 kb on - strand, within Pf1N1B4_2840 at 3185.082 kb on + strand, within Pf1N1B4_2840 at 3185.082 kb on + strand, within Pf1N1B4_2840 at 3185.082 kb on + strand, within Pf1N1B4_2840 at 3185.083 kb on - strand, within Pf1N1B4_2840 at 3185.083 kb on - strand, within Pf1N1B4_2840 at 3185.083 kb on - strand, within Pf1N1B4_2840 at 3185.083 kb on - strand, within Pf1N1B4_2840 at 3185.083 kb on - strand, within Pf1N1B4_2840 at 3185.269 kb on + strand at 3185.269 kb on + strand at 3185.270 kb on - strand at 3185.270 kb on - strand at 3185.270 kb on - strand at 3185.270 kb on - strand at 3185.270 kb on - strand at 3185.270 kb on - strand at 3185.305 kb on + strand at 3185.305 kb on + strand at 3185.306 kb on - strand at 3185.343 kb on + strand at 3185.344 kb on - strand at 3185.346 kb on + strand at 3185.346 kb on + strand at 3185.347 kb on - strand at 3185.347 kb on - strand at 3185.410 kb on + strand, within Pf1N1B4_2841 at 3185.410 kb on + strand, within Pf1N1B4_2841 at 3185.411 kb on - strand, within Pf1N1B4_2841 at 3185.411 kb on - strand, within Pf1N1B4_2841 at 3185.496 kb on + strand, within Pf1N1B4_2841 at 3185.496 kb on + strand, within Pf1N1B4_2841 at 3185.496 kb on + strand at 3185.496 kb on + strand, within Pf1N1B4_2841 at 3185.497 kb on - strand, within Pf1N1B4_2841 at 3185.497 kb on - strand, within Pf1N1B4_2841 at 3185.497 kb on - strand, within Pf1N1B4_2841 at 3185.497 kb on - strand, within Pf1N1B4_2841 at 3185.497 kb on - strand, within Pf1N1B4_2841 at 3185.497 kb on - strand, within Pf1N1B4_2841 at 3185.498 kb on + strand, within Pf1N1B4_2841 at 3185.498 kb on + strand, within Pf1N1B4_2841 at 3185.553 kb on + strand, within Pf1N1B4_2841 at 3185.553 kb on + strand, within Pf1N1B4_2841 at 3185.559 kb on + strand, within Pf1N1B4_2841 at 3185.559 kb on + strand, within Pf1N1B4_2841 at 3185.559 kb on + strand, within Pf1N1B4_2841 at 3185.559 kb on + strand at 3185.562 kb on + strand, within Pf1N1B4_2841 at 3185.562 kb on + strand, within Pf1N1B4_2841 at 3185.563 kb on - strand, within Pf1N1B4_2841 at 3185.686 kb on + strand, within Pf1N1B4_2841 at 3185.686 kb on + strand, within Pf1N1B4_2841 at 3185.687 kb on - strand, within Pf1N1B4_2841 at 3185.687 kb on - strand at 3185.687 kb on - strand, within Pf1N1B4_2841 at 3185.971 kb on + strand, within Pf1N1B4_2841 at 3185.971 kb on + strand, within Pf1N1B4_2841 at 3185.972 kb on - strand, within Pf1N1B4_2841 at 3186.022 kb on + strand, within Pf1N1B4_2841 at 3186.022 kb on + strand, within Pf1N1B4_2841 at 3186.022 kb on + strand, within Pf1N1B4_2841 at 3186.022 kb on + strand, within Pf1N1B4_2841 at 3186.023 kb on - strand, within Pf1N1B4_2841 at 3186.023 kb on - strand, within Pf1N1B4_2841 at 3186.023 kb on - strand, within Pf1N1B4_2841 at 3186.023 kb on - strand, within Pf1N1B4_2841 at 3186.023 kb on - strand, within Pf1N1B4_2841 at 3186.091 kb on + strand, within Pf1N1B4_2841 at 3186.091 kb on + strand, within Pf1N1B4_2841 at 3186.091 kb on + strand, within Pf1N1B4_2841 at 3186.091 kb on + strand at 3186.091 kb on + strand, within Pf1N1B4_2841 at 3186.106 kb on + strand, within Pf1N1B4_2841 at 3186.106 kb on + strand, within Pf1N1B4_2841 at 3186.107 kb on - strand, within Pf1N1B4_2841 at 3186.107 kb on - strand, within Pf1N1B4_2841 at 3186.166 kb on - strand, within Pf1N1B4_2841 at 3186.240 kb on + strand, within Pf1N1B4_2841
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 3,183,489 + Pf1N1B4_2837 0.62 +0.7 3,183,489 + Pf1N1B4_2837 0.62 +1.8 3,183,489 + Pf1N1B4_2837 0.62 +1.8 3,183,489 + Pf1N1B4_2837 0.62 +1.2 3,183,489 + Pf1N1B4_2837 0.62 +2.5 3,183,490 - Pf1N1B4_2837 0.62 -2.1 3,183,490 - Pf1N1B4_2837 0.62 -1.7 3,183,491 + Pf1N1B4_2837 0.63 -1.6 3,183,491 + Pf1N1B4_2837 0.63 -0.1 3,183,491 + Pf1N1B4_2837 0.63 +0.3 3,183,491 + Pf1N1B4_2837 0.63 -0.1 3,183,491 + Pf1N1B4_2837 0.63 +0.2 3,183,491 + Pf1N1B4_2837 0.63 +0.8 3,183,491 + Pf1N1B4_2837 0.63 +0.2 3,183,491 + Pf1N1B4_2837 0.63 -1.3 3,183,491 + Pf1N1B4_2837 0.63 -2.3 3,183,491 + Pf1N1B4_2837 0.63 -0.1 3,183,492 - Pf1N1B4_2837 0.63 +3.4 3,183,492 - Pf1N1B4_2837 0.63 +0.4 3,183,492 - Pf1N1B4_2837 0.63 +0.9 3,183,492 - Pf1N1B4_2837 0.63 +2.4 3,183,492 - Pf1N1B4_2837 0.63 +0.7 3,183,492 - Pf1N1B4_2837 0.63 -0.9 3,183,492 - Pf1N1B4_2837 0.63 -0.1 3,183,492 - Pf1N1B4_2837 0.63 +0.3 3,183,492 - Pf1N1B4_2837 0.63 +0.6 3,183,492 - Pf1N1B4_2837 0.63 -0.7 3,183,492 - +1.2 3,183,492 - Pf1N1B4_2837 0.63 +1.4 3,183,492 - Pf1N1B4_2837 0.63 +2.0 3,183,492 - Pf1N1B4_2837 0.63 +2.0 3,183,492 - Pf1N1B4_2837 0.63 +0.7 3,183,492 - Pf1N1B4_2837 0.63 +1.3 3,183,550 + +0.8 3,183,551 - +1.3 3,183,558 + +1.9 3,183,800 + -1.9 3,183,800 + +0.3 3,183,800 + -2.0 3,183,800 + -0.1 3,183,800 + +0.7 3,183,801 - -2.1 3,183,801 - -2.1 3,183,801 - +1.4 3,183,801 - +1.3 3,183,801 - +1.0 3,183,801 - -0.0 3,183,801 - -1.0 3,183,801 - -2.0 3,183,801 - -1.8 3,183,801 - +0.9 3,184,395 - -0.7 3,184,423 - -0.8 3,184,423 - +1.1 3,184,423 - -1.3 3,184,423 - +3.4 3,184,423 - -1.5 3,184,434 - +2.2 3,184,434 - +3.4 3,184,480 - -0.1 3,184,673 + Pf1N1B4_2840 0.32 +1.4 3,185,001 + Pf1N1B4_2840 0.69 -0.9 3,185,001 + Pf1N1B4_2840 0.69 -1.8 3,185,002 - +2.0 3,185,002 - Pf1N1B4_2840 0.69 -2.6 3,185,002 - Pf1N1B4_2840 0.69 +0.3 3,185,002 - Pf1N1B4_2840 0.69 -1.0 3,185,002 - Pf1N1B4_2840 0.69 -0.3 3,185,073 - Pf1N1B4_2840 0.77 -2.0 3,185,082 + Pf1N1B4_2840 0.78 +1.2 3,185,082 + Pf1N1B4_2840 0.78 -1.9 3,185,082 + Pf1N1B4_2840 0.78 -1.5 3,185,083 - Pf1N1B4_2840 0.79 +1.7 3,185,083 - Pf1N1B4_2840 0.79 +0.4 3,185,083 - Pf1N1B4_2840 0.79 -1.0 3,185,083 - Pf1N1B4_2840 0.79 +0.1 3,185,083 - Pf1N1B4_2840 0.79 -0.9 3,185,269 + +1.3 3,185,269 + -1.3 3,185,270 - +1.4 3,185,270 - +1.4 3,185,270 - -1.6 3,185,270 - +1.7 3,185,270 - -1.3 3,185,270 - +0.1 3,185,305 + +2.3 3,185,305 + -1.0 3,185,306 - +0.7 3,185,343 + +2.2 3,185,344 - +0.2 3,185,346 + -2.5 3,185,346 + +1.0 3,185,347 - -1.5 3,185,347 - -2.8 3,185,410 + Pf1N1B4_2841 0.11 +1.9 3,185,410 + Pf1N1B4_2841 0.11 +0.2 3,185,411 - Pf1N1B4_2841 0.11 -0.8 3,185,411 - Pf1N1B4_2841 0.11 -2.6 3,185,496 + Pf1N1B4_2841 0.18 +0.4 3,185,496 + Pf1N1B4_2841 0.18 +0.4 3,185,496 + +1.3 3,185,496 + Pf1N1B4_2841 0.18 -1.3 3,185,497 - Pf1N1B4_2841 0.19 -1.3 3,185,497 - Pf1N1B4_2841 0.19 -0.1 3,185,497 - Pf1N1B4_2841 0.19 +1.3 3,185,497 - Pf1N1B4_2841 0.19 -1.3 3,185,497 - Pf1N1B4_2841 0.19 -2.4 3,185,497 - Pf1N1B4_2841 0.19 -0.3 3,185,498 + Pf1N1B4_2841 0.19 +0.5 3,185,498 + Pf1N1B4_2841 0.19 +3.4 3,185,553 + Pf1N1B4_2841 0.23 -2.8 3,185,553 + Pf1N1B4_2841 0.23 +0.7 3,185,559 + Pf1N1B4_2841 0.24 +0.2 3,185,559 + Pf1N1B4_2841 0.24 -1.7 3,185,559 + Pf1N1B4_2841 0.24 -0.3 3,185,559 + +1.4 3,185,562 + Pf1N1B4_2841 0.24 -0.9 3,185,562 + Pf1N1B4_2841 0.24 -0.9 3,185,563 - Pf1N1B4_2841 0.24 +0.0 3,185,686 + Pf1N1B4_2841 0.34 -0.3 3,185,686 + Pf1N1B4_2841 0.34 -1.8 3,185,687 - Pf1N1B4_2841 0.34 -0.5 3,185,687 - -1.3 3,185,687 - Pf1N1B4_2841 0.34 +0.7 3,185,971 + Pf1N1B4_2841 0.57 -0.9 3,185,971 + Pf1N1B4_2841 0.57 -0.1 3,185,972 - Pf1N1B4_2841 0.58 +0.0 3,186,022 + Pf1N1B4_2841 0.62 -1.5 3,186,022 + Pf1N1B4_2841 0.62 +0.5 3,186,022 + Pf1N1B4_2841 0.62 -0.2 3,186,022 + Pf1N1B4_2841 0.62 +0.6 3,186,023 - Pf1N1B4_2841 0.62 +0.8 3,186,023 - Pf1N1B4_2841 0.62 -0.1 3,186,023 - Pf1N1B4_2841 0.62 -1.7 3,186,023 - Pf1N1B4_2841 0.62 -0.7 3,186,023 - Pf1N1B4_2841 0.62 +0.4 3,186,091 + Pf1N1B4_2841 0.67 -2.6 3,186,091 + Pf1N1B4_2841 0.67 -0.4 3,186,091 + Pf1N1B4_2841 0.67 -3.1 3,186,091 + +0.4 3,186,091 + Pf1N1B4_2841 0.67 +1.6 3,186,106 + Pf1N1B4_2841 0.69 +0.6 3,186,106 + Pf1N1B4_2841 0.69 -0.5 3,186,107 - Pf1N1B4_2841 0.69 -0.0 3,186,107 - Pf1N1B4_2841 0.69 -2.1 3,186,166 - Pf1N1B4_2841 0.73 -2.5 3,186,240 + Pf1N1B4_2841 0.80 +0.4
Or see this region's nucleotide sequence