Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2468

Experiment: nitrogen source nitrite 0.1mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2467 and Pf1N1B4_2468 are separated by 236 nucleotidesPf1N1B4_2468 and Pf1N1B4_2469 are separated by 213 nucleotides Pf1N1B4_2467 - Acetyltransferase, at 2,797,408 to 2,798,418 _2467 Pf1N1B4_2468 - Inner membrane protein CreD, at 2,798,655 to 2,800,025 _2468 Pf1N1B4_2469 - Glutathione S-transferase family protein, at 2,800,239 to 2,800,904 _2469 Position (kb) 2798 2799 2800 2801Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2797.688 kb on - strand, within Pf1N1B4_2467at 2797.729 kb on + strand, within Pf1N1B4_2467at 2797.729 kb on + strand, within Pf1N1B4_2467at 2797.730 kb on - strand, within Pf1N1B4_2467at 2797.732 kb on + strand, within Pf1N1B4_2467at 2797.733 kb on - strand, within Pf1N1B4_2467at 2797.756 kb on + strand, within Pf1N1B4_2467at 2797.757 kb on - strand, within Pf1N1B4_2467at 2797.844 kb on + strand, within Pf1N1B4_2467at 2797.880 kb on + strand, within Pf1N1B4_2467at 2797.880 kb on + strand, within Pf1N1B4_2467at 2797.880 kb on + strand, within Pf1N1B4_2467at 2797.880 kb on + strand, within Pf1N1B4_2467at 2797.880 kb on + strand, within Pf1N1B4_2467at 2797.881 kb on - strandat 2797.881 kb on - strand, within Pf1N1B4_2467at 2797.960 kb on + strand, within Pf1N1B4_2467at 2797.980 kb on - strand, within Pf1N1B4_2467at 2798.050 kb on + strand, within Pf1N1B4_2467at 2798.050 kb on + strand, within Pf1N1B4_2467at 2798.123 kb on + strandat 2798.124 kb on - strand, within Pf1N1B4_2467at 2798.124 kb on - strand, within Pf1N1B4_2467at 2798.153 kb on + strand, within Pf1N1B4_2467at 2798.153 kb on + strand, within Pf1N1B4_2467at 2798.153 kb on + strand, within Pf1N1B4_2467at 2798.154 kb on - strand, within Pf1N1B4_2467at 2798.154 kb on - strand, within Pf1N1B4_2467at 2798.154 kb on - strand, within Pf1N1B4_2467at 2798.154 kb on - strand, within Pf1N1B4_2467at 2798.243 kb on + strand, within Pf1N1B4_2467at 2798.445 kb on + strandat 2798.445 kb on + strandat 2798.445 kb on + strandat 2798.445 kb on + strandat 2798.445 kb on + strandat 2798.445 kb on + strandat 2798.446 kb on - strandat 2798.446 kb on - strandat 2798.446 kb on - strandat 2798.446 kb on - strandat 2798.446 kb on - strandat 2798.446 kb on - strandat 2798.446 kb on - strandat 2798.446 kb on - strandat 2798.446 kb on - strandat 2798.447 kb on + strandat 2798.487 kb on + strandat 2798.514 kb on - strandat 2798.566 kb on - strandat 2798.575 kb on + strandat 2798.575 kb on + strandat 2798.575 kb on + strandat 2798.575 kb on + strandat 2798.575 kb on + strandat 2798.576 kb on - strandat 2798.803 kb on - strand, within Pf1N1B4_2468at 2798.803 kb on - strand, within Pf1N1B4_2468at 2798.925 kb on + strand, within Pf1N1B4_2468at 2798.926 kb on - strand, within Pf1N1B4_2468at 2798.926 kb on - strand, within Pf1N1B4_2468at 2798.926 kb on - strand, within Pf1N1B4_2468at 2798.926 kb on - strand, within Pf1N1B4_2468at 2798.926 kb on - strand, within Pf1N1B4_2468at 2798.991 kb on + strand, within Pf1N1B4_2468at 2798.991 kb on + strand, within Pf1N1B4_2468at 2798.991 kb on + strand, within Pf1N1B4_2468at 2798.991 kb on + strand, within Pf1N1B4_2468at 2798.992 kb on - strand, within Pf1N1B4_2468at 2798.992 kb on - strand, within Pf1N1B4_2468at 2799.381 kb on + strand, within Pf1N1B4_2468at 2799.381 kb on + strand, within Pf1N1B4_2468at 2799.381 kb on + strand, within Pf1N1B4_2468at 2799.381 kb on + strandat 2799.382 kb on - strand, within Pf1N1B4_2468at 2799.382 kb on - strand, within Pf1N1B4_2468at 2799.382 kb on - strand, within Pf1N1B4_2468at 2799.382 kb on - strand, within Pf1N1B4_2468at 2799.382 kb on - strand, within Pf1N1B4_2468at 2799.382 kb on - strand, within Pf1N1B4_2468at 2799.382 kb on - strandat 2799.382 kb on - strand, within Pf1N1B4_2468at 2799.382 kb on - strand, within Pf1N1B4_2468at 2799.382 kb on - strand, within Pf1N1B4_2468at 2799.553 kb on - strand, within Pf1N1B4_2468at 2799.579 kb on + strand, within Pf1N1B4_2468at 2799.663 kb on + strand, within Pf1N1B4_2468at 2799.696 kb on + strand, within Pf1N1B4_2468at 2799.697 kb on - strand, within Pf1N1B4_2468at 2799.697 kb on - strand, within Pf1N1B4_2468at 2799.786 kb on + strand, within Pf1N1B4_2468at 2799.786 kb on + strand, within Pf1N1B4_2468at 2799.786 kb on + strand, within Pf1N1B4_2468at 2799.786 kb on + strand, within Pf1N1B4_2468at 2799.786 kb on + strand, within Pf1N1B4_2468at 2799.786 kb on + strand, within Pf1N1B4_2468at 2799.786 kb on + strand, within Pf1N1B4_2468at 2799.786 kb on + strand, within Pf1N1B4_2468at 2799.786 kb on + strand, within Pf1N1B4_2468at 2799.786 kb on + strand, within Pf1N1B4_2468at 2799.786 kb on + strand, within Pf1N1B4_2468at 2799.786 kb on + strand, within Pf1N1B4_2468at 2799.787 kb on - strand, within Pf1N1B4_2468at 2799.787 kb on - strand, within Pf1N1B4_2468at 2799.787 kb on - strand, within Pf1N1B4_2468at 2799.787 kb on - strand, within Pf1N1B4_2468at 2799.787 kb on - strand, within Pf1N1B4_2468at 2799.787 kb on - strand, within Pf1N1B4_2468at 2799.787 kb on - strand, within Pf1N1B4_2468at 2799.787 kb on - strand, within Pf1N1B4_2468at 2799.787 kb on - strand, within Pf1N1B4_2468at 2799.787 kb on - strand, within Pf1N1B4_2468at 2799.787 kb on - strand, within Pf1N1B4_2468at 2799.852 kb on + strand, within Pf1N1B4_2468at 2799.852 kb on + strand, within Pf1N1B4_2468at 2799.852 kb on + strand, within Pf1N1B4_2468at 2799.853 kb on - strand, within Pf1N1B4_2468at 2799.864 kb on + strand, within Pf1N1B4_2468at 2799.864 kb on + strand, within Pf1N1B4_2468at 2799.865 kb on - strand, within Pf1N1B4_2468at 2800.101 kb on + strandat 2800.158 kb on + strandat 2800.158 kb on + strandat 2800.159 kb on - strandat 2800.164 kb on + strandat 2800.165 kb on - strandat 2800.291 kb on + strandat 2800.291 kb on + strandat 2800.292 kb on - strandat 2800.510 kb on - strand, within Pf1N1B4_2469at 2800.510 kb on - strand, within Pf1N1B4_2469at 2800.516 kb on + strand, within Pf1N1B4_2469at 2800.627 kb on + strand, within Pf1N1B4_2469at 2800.636 kb on + strand, within Pf1N1B4_2469at 2800.660 kb on + strand, within Pf1N1B4_2469at 2800.660 kb on + strandat 2800.660 kb on + strand, within Pf1N1B4_2469at 2800.661 kb on - strand, within Pf1N1B4_2469at 2800.661 kb on - strand, within Pf1N1B4_2469at 2800.845 kb on + strandat 2800.845 kb on + strandat 2800.845 kb on + strandat 2800.845 kb on + strandat 2800.845 kb on + strandat 2800.846 kb on - strandat 2801.004 kb on + strandat 2801.004 kb on + strandat 2801.004 kb on + strandat 2801.004 kb on + strandat 2801.005 kb on - strandat 2801.005 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM
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2,797,688 - Pf1N1B4_2467 0.28 -2.2
2,797,729 + Pf1N1B4_2467 0.32 +2.9
2,797,729 + Pf1N1B4_2467 0.32 +2.5
2,797,730 - Pf1N1B4_2467 0.32 -2.7
2,797,732 + Pf1N1B4_2467 0.32 +2.2
2,797,733 - Pf1N1B4_2467 0.32 +2.2
2,797,756 + Pf1N1B4_2467 0.34 -1.4
2,797,757 - Pf1N1B4_2467 0.35 -2.4
2,797,844 + Pf1N1B4_2467 0.43 +1.4
2,797,880 + Pf1N1B4_2467 0.47 +0.4
2,797,880 + Pf1N1B4_2467 0.47 +0.9
2,797,880 + Pf1N1B4_2467 0.47 +1.1
2,797,880 + Pf1N1B4_2467 0.47 +1.2
2,797,880 + Pf1N1B4_2467 0.47 -0.6
2,797,881 - +0.2
2,797,881 - Pf1N1B4_2467 0.47 -2.5
2,797,960 + Pf1N1B4_2467 0.55 -1.1
2,797,980 - Pf1N1B4_2467 0.57 -2.0
2,798,050 + Pf1N1B4_2467 0.64 +1.5
2,798,050 + Pf1N1B4_2467 0.64 -1.9
2,798,123 + -0.7
2,798,124 - Pf1N1B4_2467 0.71 -2.5
2,798,124 - Pf1N1B4_2467 0.71 +1.8
2,798,153 + Pf1N1B4_2467 0.74 +0.7
2,798,153 + Pf1N1B4_2467 0.74 +0.2
2,798,153 + Pf1N1B4_2467 0.74 -1.6
2,798,154 - Pf1N1B4_2467 0.74 -0.7
2,798,154 - Pf1N1B4_2467 0.74 -1.3
2,798,154 - Pf1N1B4_2467 0.74 -0.2
2,798,154 - Pf1N1B4_2467 0.74 -0.6
2,798,243 + Pf1N1B4_2467 0.83 -0.8
2,798,445 + -2.3
2,798,445 + -3.3
2,798,445 + -2.8
2,798,445 + -1.4
2,798,445 + -0.2
2,798,445 + -0.8
2,798,446 - -1.3
2,798,446 - -1.1
2,798,446 - -2.0
2,798,446 - +1.3
2,798,446 - +1.9
2,798,446 - +0.4
2,798,446 - -2.0
2,798,446 - -0.8
2,798,446 - -2.2
2,798,447 + +0.5
2,798,487 + +0.4
2,798,514 - -1.0
2,798,566 - -0.4
2,798,575 + -1.6
2,798,575 + -1.2
2,798,575 + +0.5
2,798,575 + -2.6
2,798,575 + +1.2
2,798,576 - +1.9
2,798,803 - Pf1N1B4_2468 0.11 +0.6
2,798,803 - Pf1N1B4_2468 0.11 +1.2
2,798,925 + Pf1N1B4_2468 0.20 +0.3
2,798,926 - Pf1N1B4_2468 0.20 -2.2
2,798,926 - Pf1N1B4_2468 0.20 -2.3
2,798,926 - Pf1N1B4_2468 0.20 -2.4
2,798,926 - Pf1N1B4_2468 0.20 -0.4
2,798,926 - Pf1N1B4_2468 0.20 +0.7
2,798,991 + Pf1N1B4_2468 0.25 +0.3
2,798,991 + Pf1N1B4_2468 0.25 -1.6
2,798,991 + Pf1N1B4_2468 0.25 -1.8
2,798,991 + Pf1N1B4_2468 0.25 -0.4
2,798,992 - Pf1N1B4_2468 0.25 -1.0
2,798,992 - Pf1N1B4_2468 0.25 +2.6
2,799,381 + Pf1N1B4_2468 0.53 -0.8
2,799,381 + Pf1N1B4_2468 0.53 +0.5
2,799,381 + Pf1N1B4_2468 0.53 -1.2
2,799,381 + -0.5
2,799,382 - Pf1N1B4_2468 0.53 -2.3
2,799,382 - Pf1N1B4_2468 0.53 +1.4
2,799,382 - Pf1N1B4_2468 0.53 -1.2
2,799,382 - Pf1N1B4_2468 0.53 +1.5
2,799,382 - Pf1N1B4_2468 0.53 +2.1
2,799,382 - Pf1N1B4_2468 0.53 +1.3
2,799,382 - -0.7
2,799,382 - Pf1N1B4_2468 0.53 +1.3
2,799,382 - Pf1N1B4_2468 0.53 -1.0
2,799,382 - Pf1N1B4_2468 0.53 +3.2
2,799,553 - Pf1N1B4_2468 0.65 -0.1
2,799,579 + Pf1N1B4_2468 0.67 +1.1
2,799,663 + Pf1N1B4_2468 0.74 -0.6
2,799,696 + Pf1N1B4_2468 0.76 +1.0
2,799,697 - Pf1N1B4_2468 0.76 -2.3
2,799,697 - Pf1N1B4_2468 0.76 -2.4
2,799,786 + Pf1N1B4_2468 0.82 -0.7
2,799,786 + Pf1N1B4_2468 0.82 +0.6
2,799,786 + Pf1N1B4_2468 0.82 -0.1
2,799,786 + Pf1N1B4_2468 0.82 -0.5
2,799,786 + Pf1N1B4_2468 0.82 -0.7
2,799,786 + Pf1N1B4_2468 0.82 -0.5
2,799,786 + Pf1N1B4_2468 0.82 +0.9
2,799,786 + Pf1N1B4_2468 0.82 -2.2
2,799,786 + Pf1N1B4_2468 0.82 -0.4
2,799,786 + Pf1N1B4_2468 0.82 +2.0
2,799,786 + Pf1N1B4_2468 0.82 +1.7
2,799,786 + Pf1N1B4_2468 0.82 -1.6
2,799,787 - Pf1N1B4_2468 0.83 +0.2
2,799,787 - Pf1N1B4_2468 0.83 +1.6
2,799,787 - Pf1N1B4_2468 0.83 +1.3
2,799,787 - Pf1N1B4_2468 0.83 +3.7
2,799,787 - Pf1N1B4_2468 0.83 -1.5
2,799,787 - Pf1N1B4_2468 0.83 -1.0
2,799,787 - Pf1N1B4_2468 0.83 +1.1
2,799,787 - Pf1N1B4_2468 0.83 -0.3
2,799,787 - Pf1N1B4_2468 0.83 -1.0
2,799,787 - Pf1N1B4_2468 0.83 -1.6
2,799,787 - Pf1N1B4_2468 0.83 -2.8
2,799,852 + Pf1N1B4_2468 0.87 -2.4
2,799,852 + Pf1N1B4_2468 0.87 +2.1
2,799,852 + Pf1N1B4_2468 0.87 -1.5
2,799,853 - Pf1N1B4_2468 0.87 -2.7
2,799,864 + Pf1N1B4_2468 0.88 -0.5
2,799,864 + Pf1N1B4_2468 0.88 -0.5
2,799,865 - Pf1N1B4_2468 0.88 +0.4
2,800,101 + -0.8
2,800,158 + -1.9
2,800,158 + +1.6
2,800,159 - -2.7
2,800,164 + -0.1
2,800,165 - +0.5
2,800,291 + -0.7
2,800,291 + -0.8
2,800,292 - -2.7
2,800,510 - Pf1N1B4_2469 0.41 +0.2
2,800,510 - Pf1N1B4_2469 0.41 -2.4
2,800,516 + Pf1N1B4_2469 0.42 +0.6
2,800,627 + Pf1N1B4_2469 0.58 +0.5
2,800,636 + Pf1N1B4_2469 0.60 +0.9
2,800,660 + Pf1N1B4_2469 0.63 -1.6
2,800,660 + +2.3
2,800,660 + Pf1N1B4_2469 0.63 -2.7
2,800,661 - Pf1N1B4_2469 0.63 +1.8
2,800,661 - Pf1N1B4_2469 0.63 -2.4
2,800,845 + -0.7
2,800,845 + -0.8
2,800,845 + -0.4
2,800,845 + +2.9
2,800,845 + +2.9
2,800,846 - +0.1
2,801,004 + +2.2
2,801,004 + -3.3
2,801,004 + -0.7
2,801,004 + -2.4
2,801,005 - -0.7
2,801,005 - +0.2

Or see this region's nucleotide sequence