Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_243 and Pf1N1B4_244 overlap by 105 nucleotides Pf1N1B4_244 and Pf1N1B4_245 are separated by 219 nucleotides Pf1N1B4_245 and Pf1N1B4_246 are separated by 1 nucleotides Pf1N1B4_246 and Pf1N1B4_247 overlap by 4 nucleotides Pf1N1B4_247 and Pf1N1B4_248 are separated by 123 nucleotides
Pf1N1B4_243 - Ammonium transporter, at 301,651 to 302,925
_243
Pf1N1B4_244 - hypothetical protein, at 302,821 to 302,946
_244
Pf1N1B4_245 - Probable transmembrane protein, at 303,166 to 303,480
_245
Pf1N1B4_246 - Probable transmembrane protein, at 303,482 to 303,871
_246
Pf1N1B4_247 - FIG00956101: hypothetical protein, at 303,868 to 304,206
_247
Pf1N1B4_248 - EAL-domain protein, at 304,330 to 305,493
_248
Position (kb) 303
304 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 302.588 kb on + strand, within Pf1N1B4_243 at 302.656 kb on + strand, within Pf1N1B4_243 at 302.656 kb on + strand, within Pf1N1B4_243 at 302.656 kb on + strand at 302.656 kb on + strand, within Pf1N1B4_243 at 302.657 kb on - strand, within Pf1N1B4_243 at 302.657 kb on - strand, within Pf1N1B4_243 at 302.657 kb on - strand at 302.658 kb on + strand, within Pf1N1B4_243 at 302.658 kb on + strand, within Pf1N1B4_243 at 302.658 kb on + strand, within Pf1N1B4_243 at 302.659 kb on - strand, within Pf1N1B4_243 at 302.659 kb on - strand, within Pf1N1B4_243 at 302.666 kb on + strand, within Pf1N1B4_243 at 302.666 kb on + strand, within Pf1N1B4_243 at 302.667 kb on - strand, within Pf1N1B4_243 at 302.820 kb on + strand at 302.820 kb on + strand at 302.820 kb on + strand at 302.821 kb on - strand at 303.008 kb on + strand at 303.009 kb on - strand at 303.079 kb on + strand at 303.080 kb on - strand at 303.104 kb on + strand at 303.105 kb on - strand at 303.128 kb on + strand at 303.128 kb on + strand at 303.129 kb on - strand at 303.160 kb on + strand at 303.397 kb on + strand, within Pf1N1B4_245 at 303.397 kb on + strand, within Pf1N1B4_245 at 303.482 kb on - strand at 303.482 kb on - strand at 303.716 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.753 kb on - strand, within Pf1N1B4_246 at 304.204 kb on + strand at 304.247 kb on + strand at 304.315 kb on + strand at 304.315 kb on + strand at 304.316 kb on - strand at 304.318 kb on + strand at 304.318 kb on + strand at 304.319 kb on - strand at 304.319 kb on - strand at 304.319 kb on - strand at 304.574 kb on + strand, within Pf1N1B4_248 at 304.575 kb on - strand, within Pf1N1B4_248
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 302,588 + Pf1N1B4_243 0.73 -0.7 302,656 + Pf1N1B4_243 0.79 -0.4 302,656 + Pf1N1B4_243 0.79 +0.6 302,656 + -2.3 302,656 + Pf1N1B4_243 0.79 -1.3 302,657 - Pf1N1B4_243 0.79 +0.6 302,657 - Pf1N1B4_243 0.79 -2.5 302,657 - +1.2 302,658 + Pf1N1B4_243 0.79 +1.3 302,658 + Pf1N1B4_243 0.79 -1.0 302,658 + Pf1N1B4_243 0.79 -3.0 302,659 - Pf1N1B4_243 0.79 +1.1 302,659 - Pf1N1B4_243 0.79 +1.8 302,666 + Pf1N1B4_243 0.80 -0.4 302,666 + Pf1N1B4_243 0.80 -0.2 302,667 - Pf1N1B4_243 0.80 +1.6 302,820 + -1.5 302,820 + -0.5 302,820 + -2.5 302,821 - +1.0 303,008 + -0.6 303,009 - +0.2 303,079 + -1.1 303,080 - +1.0 303,104 + -1.0 303,105 - +0.0 303,128 + -1.5 303,128 + -2.9 303,129 - +0.3 303,160 + +2.0 303,397 + Pf1N1B4_245 0.73 -1.9 303,397 + Pf1N1B4_245 0.73 -2.2 303,482 - +1.0 303,482 - -1.0 303,716 + Pf1N1B4_246 0.60 +2.5 303,752 + Pf1N1B4_246 0.69 +0.4 303,752 + Pf1N1B4_246 0.69 -3.0 303,752 + Pf1N1B4_246 0.69 +2.5 303,752 + Pf1N1B4_246 0.69 -2.8 303,752 + Pf1N1B4_246 0.69 +1.2 303,752 + Pf1N1B4_246 0.69 -2.9 303,752 + Pf1N1B4_246 0.69 +1.3 303,752 + Pf1N1B4_246 0.69 -0.8 303,752 + Pf1N1B4_246 0.69 +0.6 303,752 + Pf1N1B4_246 0.69 +0.3 303,753 - Pf1N1B4_246 0.69 -2.3 304,204 + -1.5 304,247 + -0.2 304,315 + +1.2 304,315 + +0.3 304,316 - -0.4 304,318 + +0.1 304,318 + -1.6 304,319 - +1.0 304,319 - +0.3 304,319 - +1.0 304,574 + Pf1N1B4_248 0.21 -2.2 304,575 - Pf1N1B4_248 0.21 -0.4
Or see this region's nucleotide sequence