Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1952 and Pf1N1B4_1953 are separated by 40 nucleotides Pf1N1B4_1953 and Pf1N1B4_1954 are separated by 159 nucleotides Pf1N1B4_1954 and Pf1N1B4_1955 are separated by 131 nucleotides Pf1N1B4_1955 and Pf1N1B4_1956 are separated by 150 nucleotides
Pf1N1B4_1952 - Putative lipase in cluster with Phosphatidate cytidylyltransferase, at 2,232,632 to 2,234,389
_1952
Pf1N1B4_1953 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (EC 2.7.8.5), at 2,234,430 to 2,235,053
_1953
Pf1N1B4_1954 - Transcriptional regulator, at 2,235,213 to 2,236,115
_1954
Pf1N1B4_1955 - Glutathione S-transferase, unnamed subgroup (EC 2.5.1.18), at 2,236,247 to 2,236,873
_1955
Pf1N1B4_1956 - Flavohemoprotein (Hemoglobin-like protein) (Flavohemoglobin) (Nitric oxide dioxygenase) (EC 1.14.12.17), at 2,237,024 to 2,237,890
_1956
Position (kb)
2235
2236
2237 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2234.334 kb on + strand at 2234.371 kb on + strand at 2234.372 kb on - strand at 2234.372 kb on - strand at 2234.401 kb on + strand at 2234.417 kb on + strand at 2234.417 kb on + strand at 2234.418 kb on - strand at 2234.418 kb on - strand at 2234.418 kb on - strand at 2234.431 kb on + strand at 2234.431 kb on + strand at 2234.432 kb on - strand at 2234.432 kb on - strand at 2234.494 kb on + strand, within Pf1N1B4_1953 at 2234.495 kb on - strand, within Pf1N1B4_1953 at 2234.495 kb on - strand, within Pf1N1B4_1953 at 2234.495 kb on - strand, within Pf1N1B4_1953 at 2234.495 kb on - strand, within Pf1N1B4_1953 at 2234.495 kb on - strand, within Pf1N1B4_1953 at 2234.586 kb on + strand, within Pf1N1B4_1953 at 2234.587 kb on - strand, within Pf1N1B4_1953 at 2234.623 kb on + strand, within Pf1N1B4_1953 at 2234.623 kb on + strand, within Pf1N1B4_1953 at 2234.624 kb on - strand, within Pf1N1B4_1953 at 2234.665 kb on + strand, within Pf1N1B4_1953 at 2234.666 kb on - strand, within Pf1N1B4_1953 at 2234.666 kb on - strand, within Pf1N1B4_1953 at 2234.780 kb on + strand, within Pf1N1B4_1953 at 2234.785 kb on + strand, within Pf1N1B4_1953 at 2234.878 kb on - strand, within Pf1N1B4_1953 at 2234.886 kb on + strand, within Pf1N1B4_1953 at 2234.886 kb on + strand, within Pf1N1B4_1953 at 2235.032 kb on + strand at 2235.032 kb on + strand at 2235.032 kb on + strand at 2235.032 kb on + strand at 2235.032 kb on + strand at 2235.032 kb on + strand at 2235.033 kb on - strand at 2235.033 kb on - strand at 2235.033 kb on - strand at 2235.033 kb on - strand at 2235.033 kb on - strand at 2235.040 kb on + strand at 2235.040 kb on + strand at 2235.040 kb on + strand at 2235.040 kb on + strand at 2235.040 kb on + strand at 2235.041 kb on - strand at 2235.041 kb on - strand at 2235.041 kb on - strand at 2235.041 kb on - strand at 2235.041 kb on - strand at 2235.041 kb on - strand at 2235.041 kb on - strand at 2235.096 kb on + strand at 2235.097 kb on - strand at 2235.098 kb on + strand at 2235.098 kb on + strand at 2235.098 kb on + strand at 2235.098 kb on + strand at 2235.098 kb on + strand at 2235.098 kb on + strand at 2235.098 kb on + strand at 2235.098 kb on + strand at 2235.098 kb on + strand at 2235.098 kb on + strand at 2235.098 kb on + strand at 2235.098 kb on + strand at 2235.098 kb on + strand at 2235.098 kb on + strand at 2235.098 kb on + strand at 2235.098 kb on + strand at 2235.099 kb on - strand at 2235.099 kb on - strand at 2235.099 kb on - strand at 2235.099 kb on - strand at 2235.099 kb on - strand at 2235.099 kb on - strand at 2235.099 kb on - strand at 2235.099 kb on - strand at 2235.099 kb on - strand at 2235.099 kb on - strand at 2235.099 kb on - strand at 2235.099 kb on - strand at 2235.099 kb on - strand at 2235.099 kb on - strand at 2235.099 kb on - strand at 2235.099 kb on - strand at 2235.099 kb on - strand at 2235.099 kb on - strand at 2235.099 kb on - strand at 2235.099 kb on - strand at 2235.099 kb on - strand at 2235.099 kb on - strand at 2235.108 kb on + strand at 2235.108 kb on + strand at 2235.123 kb on + strand at 2235.124 kb on - strand at 2235.207 kb on + strand at 2235.208 kb on - strand at 2235.208 kb on - strand at 2235.365 kb on + strand, within Pf1N1B4_1954 at 2235.365 kb on + strand, within Pf1N1B4_1954 at 2235.365 kb on + strand, within Pf1N1B4_1954 at 2235.365 kb on + strand, within Pf1N1B4_1954 at 2235.365 kb on + strand, within Pf1N1B4_1954 at 2235.365 kb on + strand, within Pf1N1B4_1954 at 2235.365 kb on + strand, within Pf1N1B4_1954 at 2235.366 kb on - strand, within Pf1N1B4_1954 at 2235.366 kb on - strand, within Pf1N1B4_1954 at 2235.366 kb on - strand, within Pf1N1B4_1954 at 2235.366 kb on - strand, within Pf1N1B4_1954 at 2235.366 kb on - strand, within Pf1N1B4_1954 at 2235.366 kb on - strand, within Pf1N1B4_1954 at 2235.366 kb on - strand, within Pf1N1B4_1954 at 2235.552 kb on + strand, within Pf1N1B4_1954 at 2235.553 kb on - strand, within Pf1N1B4_1954 at 2235.553 kb on - strand, within Pf1N1B4_1954 at 2235.553 kb on - strand, within Pf1N1B4_1954 at 2235.937 kb on - strand, within Pf1N1B4_1954 at 2235.937 kb on - strand, within Pf1N1B4_1954 at 2236.058 kb on + strand at 2236.059 kb on - strand at 2236.139 kb on - strand at 2236.139 kb on - strand at 2236.189 kb on + strand at 2236.265 kb on + strand at 2236.265 kb on + strand at 2236.265 kb on + strand at 2236.265 kb on + strand at 2236.265 kb on + strand at 2236.299 kb on + strand at 2236.299 kb on + strand at 2236.299 kb on + strand at 2236.299 kb on + strand at 2236.299 kb on + strand at 2236.299 kb on + strand at 2236.300 kb on - strand at 2236.466 kb on + strand, within Pf1N1B4_1955 at 2236.466 kb on + strand, within Pf1N1B4_1955 at 2236.467 kb on - strand, within Pf1N1B4_1955 at 2236.467 kb on - strand, within Pf1N1B4_1955 at 2236.481 kb on + strand, within Pf1N1B4_1955 at 2236.599 kb on + strand, within Pf1N1B4_1955 at 2236.599 kb on + strand, within Pf1N1B4_1955 at 2236.600 kb on - strand, within Pf1N1B4_1955 at 2236.600 kb on - strand at 2236.600 kb on - strand, within Pf1N1B4_1955 at 2236.600 kb on - strand, within Pf1N1B4_1955 at 2236.600 kb on - strand, within Pf1N1B4_1955 at 2236.613 kb on + strand, within Pf1N1B4_1955 at 2236.613 kb on + strand, within Pf1N1B4_1955 at 2236.613 kb on + strand, within Pf1N1B4_1955 at 2236.613 kb on + strand, within Pf1N1B4_1955 at 2236.613 kb on + strand, within Pf1N1B4_1955 at 2236.614 kb on - strand, within Pf1N1B4_1955 at 2236.614 kb on - strand, within Pf1N1B4_1955 at 2236.614 kb on - strand, within Pf1N1B4_1955 at 2236.659 kb on + strand, within Pf1N1B4_1955 at 2236.659 kb on + strand, within Pf1N1B4_1955 at 2236.659 kb on + strand, within Pf1N1B4_1955 at 2236.660 kb on - strand, within Pf1N1B4_1955 at 2236.660 kb on - strand, within Pf1N1B4_1955 at 2236.688 kb on + strand, within Pf1N1B4_1955 at 2236.688 kb on + strand, within Pf1N1B4_1955 at 2236.688 kb on + strand, within Pf1N1B4_1955 at 2236.689 kb on - strand, within Pf1N1B4_1955 at 2236.730 kb on + strand, within Pf1N1B4_1955 at 2236.736 kb on + strand, within Pf1N1B4_1955 at 2236.736 kb on + strand, within Pf1N1B4_1955 at 2236.736 kb on + strand, within Pf1N1B4_1955 at 2236.736 kb on + strand, within Pf1N1B4_1955 at 2236.736 kb on + strand, within Pf1N1B4_1955 at 2236.736 kb on + strand, within Pf1N1B4_1955 at 2236.736 kb on + strand, within Pf1N1B4_1955 at 2236.736 kb on + strand, within Pf1N1B4_1955 at 2236.736 kb on + strand, within Pf1N1B4_1955 at 2236.736 kb on + strand, within Pf1N1B4_1955 at 2236.736 kb on + strand, within Pf1N1B4_1955 at 2236.736 kb on + strand, within Pf1N1B4_1955 at 2236.737 kb on - strand at 2236.737 kb on - strand, within Pf1N1B4_1955 at 2236.737 kb on - strand, within Pf1N1B4_1955 at 2236.737 kb on - strand, within Pf1N1B4_1955 at 2236.737 kb on - strand, within Pf1N1B4_1955 at 2236.737 kb on - strand, within Pf1N1B4_1955 at 2237.013 kb on + strand at 2237.013 kb on + strand at 2237.013 kb on + strand at 2237.013 kb on + strand at 2237.014 kb on - strand at 2237.014 kb on - strand at 2237.014 kb on - strand at 2237.014 kb on - strand at 2237.020 kb on + strand at 2237.020 kb on + strand at 2237.020 kb on + strand at 2237.021 kb on - strand at 2237.063 kb on + strand at 2237.063 kb on + strand at 2237.063 kb on + strand at 2237.064 kb on - strand at 2237.064 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 2,234,334 + -0.0 2,234,371 + -1.3 2,234,372 - +0.4 2,234,372 - -1.8 2,234,401 + +2.0 2,234,417 + +1.4 2,234,417 + +0.3 2,234,418 - -0.9 2,234,418 - -0.9 2,234,418 - +0.4 2,234,431 + -1.7 2,234,431 + +0.4 2,234,432 - +0.2 2,234,432 - +1.3 2,234,494 + Pf1N1B4_1953 0.10 +0.9 2,234,495 - Pf1N1B4_1953 0.10 -1.6 2,234,495 - Pf1N1B4_1953 0.10 -0.6 2,234,495 - Pf1N1B4_1953 0.10 -3.1 2,234,495 - Pf1N1B4_1953 0.10 -0.5 2,234,495 - Pf1N1B4_1953 0.10 -0.9 2,234,586 + Pf1N1B4_1953 0.25 +1.0 2,234,587 - Pf1N1B4_1953 0.25 +0.7 2,234,623 + Pf1N1B4_1953 0.31 -0.6 2,234,623 + Pf1N1B4_1953 0.31 -1.5 2,234,624 - Pf1N1B4_1953 0.31 -1.9 2,234,665 + Pf1N1B4_1953 0.38 -0.3 2,234,666 - Pf1N1B4_1953 0.38 -0.1 2,234,666 - Pf1N1B4_1953 0.38 -0.3 2,234,780 + Pf1N1B4_1953 0.56 -0.1 2,234,785 + Pf1N1B4_1953 0.57 -1.3 2,234,878 - Pf1N1B4_1953 0.72 -2.8 2,234,886 + Pf1N1B4_1953 0.73 -0.5 2,234,886 + Pf1N1B4_1953 0.73 -1.3 2,235,032 + -2.6 2,235,032 + -1.0 2,235,032 + +0.6 2,235,032 + +0.9 2,235,032 + -1.0 2,235,032 + -1.6 2,235,033 - +0.9 2,235,033 - +1.8 2,235,033 - -1.1 2,235,033 - +3.9 2,235,033 - +0.1 2,235,040 + -2.3 2,235,040 + -0.9 2,235,040 + -1.6 2,235,040 + -0.6 2,235,040 + +0.6 2,235,041 - +1.1 2,235,041 - +0.1 2,235,041 - -1.7 2,235,041 - -1.5 2,235,041 - -1.5 2,235,041 - -1.0 2,235,041 - -0.9 2,235,096 + +1.1 2,235,097 - -0.9 2,235,098 + -0.1 2,235,098 + -0.6 2,235,098 + -1.1 2,235,098 + -2.1 2,235,098 + +1.3 2,235,098 + -0.6 2,235,098 + +1.6 2,235,098 + +1.6 2,235,098 + -2.9 2,235,098 + -0.3 2,235,098 + -2.9 2,235,098 + +0.4 2,235,098 + +1.4 2,235,098 + +0.4 2,235,098 + +1.0 2,235,098 + +0.1 2,235,099 - -1.0 2,235,099 - -1.1 2,235,099 - +1.1 2,235,099 - +1.3 2,235,099 - +0.9 2,235,099 - +1.0 2,235,099 - +1.7 2,235,099 - -1.1 2,235,099 - +0.6 2,235,099 - +1.6 2,235,099 - -0.0 2,235,099 - +2.8 2,235,099 - +0.9 2,235,099 - +1.1 2,235,099 - -2.1 2,235,099 - -3.4 2,235,099 - -1.1 2,235,099 - -1.7 2,235,099 - -0.8 2,235,099 - -0.9 2,235,099 - -1.8 2,235,099 - +1.3 2,235,108 + +1.9 2,235,108 + -2.3 2,235,123 + -0.5 2,235,124 - -2.3 2,235,207 + -0.3 2,235,208 - -1.8 2,235,208 - -1.6 2,235,365 + Pf1N1B4_1954 0.17 -0.6 2,235,365 + Pf1N1B4_1954 0.17 -2.1 2,235,365 + Pf1N1B4_1954 0.17 -0.6 2,235,365 + Pf1N1B4_1954 0.17 +1.6 2,235,365 + Pf1N1B4_1954 0.17 -3.6 2,235,365 + Pf1N1B4_1954 0.17 -0.9 2,235,365 + Pf1N1B4_1954 0.17 -3.2 2,235,366 - Pf1N1B4_1954 0.17 +2.2 2,235,366 - Pf1N1B4_1954 0.17 +0.1 2,235,366 - Pf1N1B4_1954 0.17 -1.0 2,235,366 - Pf1N1B4_1954 0.17 -0.3 2,235,366 - Pf1N1B4_1954 0.17 +1.9 2,235,366 - Pf1N1B4_1954 0.17 -2.0 2,235,366 - Pf1N1B4_1954 0.17 +1.4 2,235,552 + Pf1N1B4_1954 0.38 -2.4 2,235,553 - Pf1N1B4_1954 0.38 +1.3 2,235,553 - Pf1N1B4_1954 0.38 +0.5 2,235,553 - Pf1N1B4_1954 0.38 -0.1 2,235,937 - Pf1N1B4_1954 0.80 -2.8 2,235,937 - Pf1N1B4_1954 0.80 -1.9 2,236,058 + +0.9 2,236,059 - -1.5 2,236,139 - -1.1 2,236,139 - +1.3 2,236,189 + +0.3 2,236,265 + -2.1 2,236,265 + -0.4 2,236,265 + +1.3 2,236,265 + -0.2 2,236,265 + -1.3 2,236,299 + -0.7 2,236,299 + +0.9 2,236,299 + +1.6 2,236,299 + -0.3 2,236,299 + -2.1 2,236,299 + -2.5 2,236,300 - -0.1 2,236,466 + Pf1N1B4_1955 0.35 -3.1 2,236,466 + Pf1N1B4_1955 0.35 +0.6 2,236,467 - Pf1N1B4_1955 0.35 +0.0 2,236,467 - Pf1N1B4_1955 0.35 -0.4 2,236,481 + Pf1N1B4_1955 0.37 -0.4 2,236,599 + Pf1N1B4_1955 0.56 +3.3 2,236,599 + Pf1N1B4_1955 0.56 -2.1 2,236,600 - Pf1N1B4_1955 0.56 -0.4 2,236,600 - -1.6 2,236,600 - Pf1N1B4_1955 0.56 +0.0 2,236,600 - Pf1N1B4_1955 0.56 -1.3 2,236,600 - Pf1N1B4_1955 0.56 -2.2 2,236,613 + Pf1N1B4_1955 0.58 -1.5 2,236,613 + Pf1N1B4_1955 0.58 +0.7 2,236,613 + Pf1N1B4_1955 0.58 -1.6 2,236,613 + Pf1N1B4_1955 0.58 -1.3 2,236,613 + Pf1N1B4_1955 0.58 +2.8 2,236,614 - Pf1N1B4_1955 0.59 +1.7 2,236,614 - Pf1N1B4_1955 0.59 -0.3 2,236,614 - Pf1N1B4_1955 0.59 -1.9 2,236,659 + Pf1N1B4_1955 0.66 -1.3 2,236,659 + Pf1N1B4_1955 0.66 -1.6 2,236,659 + Pf1N1B4_1955 0.66 +0.2 2,236,660 - Pf1N1B4_1955 0.66 +0.8 2,236,660 - Pf1N1B4_1955 0.66 +0.9 2,236,688 + Pf1N1B4_1955 0.70 -0.6 2,236,688 + Pf1N1B4_1955 0.70 +2.6 2,236,688 + Pf1N1B4_1955 0.70 +1.2 2,236,689 - Pf1N1B4_1955 0.70 +1.3 2,236,730 + Pf1N1B4_1955 0.77 -1.4 2,236,736 + Pf1N1B4_1955 0.78 +0.1 2,236,736 + Pf1N1B4_1955 0.78 -0.7 2,236,736 + Pf1N1B4_1955 0.78 +1.4 2,236,736 + Pf1N1B4_1955 0.78 -1.8 2,236,736 + Pf1N1B4_1955 0.78 -0.7 2,236,736 + Pf1N1B4_1955 0.78 +2.3 2,236,736 + Pf1N1B4_1955 0.78 -3.2 2,236,736 + Pf1N1B4_1955 0.78 -0.9 2,236,736 + Pf1N1B4_1955 0.78 +0.6 2,236,736 + Pf1N1B4_1955 0.78 +0.3 2,236,736 + Pf1N1B4_1955 0.78 +0.6 2,236,736 + Pf1N1B4_1955 0.78 -1.8 2,236,737 - +1.0 2,236,737 - Pf1N1B4_1955 0.78 -0.9 2,236,737 - Pf1N1B4_1955 0.78 -2.6 2,236,737 - Pf1N1B4_1955 0.78 -0.9 2,236,737 - Pf1N1B4_1955 0.78 -1.8 2,236,737 - Pf1N1B4_1955 0.78 -2.1 2,237,013 + +0.7 2,237,013 + -3.1 2,237,013 + -1.6 2,237,013 + +0.9 2,237,014 - +0.1 2,237,014 - -3.1 2,237,014 - +0.1 2,237,014 - -2.9 2,237,020 + +2.4 2,237,020 + +2.7 2,237,020 + +0.2 2,237,021 - +0.6 2,237,063 + +0.8 2,237,063 + -0.7 2,237,063 + -0.6 2,237,064 - +1.1 2,237,064 - -1.0
Or see this region's nucleotide sequence