Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1591 and Pf1N1B4_1592 are separated by 118 nucleotides Pf1N1B4_1592 and Pf1N1B4_1593 are separated by 33 nucleotides Pf1N1B4_1593 and Pf1N1B4_1594 are separated by 291 nucleotides
Pf1N1B4_1591 - Formiminoglutamic iminohydrolase (EC 3.5.3.13) (from data) , at 1,832,279 to 1,833,643
_1591
Pf1N1B4_1592 - Outer membrane lipoprotein Blc, at 1,833,762 to 1,834,331
_1592
Pf1N1B4_1593 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 1,834,365 to 1,834,619
_1593
Pf1N1B4_1594 - Methyl-accepting chemotaxis protein, at 1,834,911 to 1,836,791
_1594
Position (kb)
1834
1835 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1833.467 kb on + strand, within Pf1N1B4_1591 at 1833.467 kb on + strand, within Pf1N1B4_1591 at 1833.601 kb on + strand at 1833.602 kb on - strand at 1833.745 kb on + strand at 1833.745 kb on + strand at 1833.745 kb on + strand at 1833.745 kb on + strand at 1833.745 kb on + strand at 1833.746 kb on - strand at 1833.746 kb on - strand at 1833.746 kb on - strand at 1833.746 kb on - strand at 1833.746 kb on - strand at 1833.746 kb on - strand at 1833.746 kb on - strand at 1833.746 kb on - strand at 1833.763 kb on + strand at 1833.763 kb on + strand at 1833.763 kb on + strand at 1833.764 kb on - strand at 1833.764 kb on - strand at 1833.764 kb on - strand at 1833.764 kb on - strand at 1833.789 kb on - strand at 1833.940 kb on + strand, within Pf1N1B4_1592 at 1833.940 kb on + strand, within Pf1N1B4_1592 at 1833.940 kb on + strand, within Pf1N1B4_1592 at 1833.940 kb on + strand, within Pf1N1B4_1592 at 1833.941 kb on - strand, within Pf1N1B4_1592 at 1833.941 kb on - strand, within Pf1N1B4_1592 at 1833.941 kb on - strand, within Pf1N1B4_1592 at 1833.941 kb on - strand, within Pf1N1B4_1592 at 1833.955 kb on + strand, within Pf1N1B4_1592 at 1833.955 kb on + strand, within Pf1N1B4_1592 at 1833.955 kb on + strand, within Pf1N1B4_1592 at 1833.956 kb on - strand, within Pf1N1B4_1592 at 1833.956 kb on - strand, within Pf1N1B4_1592 at 1833.956 kb on - strand, within Pf1N1B4_1592 at 1833.956 kb on - strand, within Pf1N1B4_1592 at 1833.967 kb on + strand, within Pf1N1B4_1592 at 1833.968 kb on - strand, within Pf1N1B4_1592 at 1833.968 kb on - strand, within Pf1N1B4_1592 at 1833.968 kb on - strand, within Pf1N1B4_1592 at 1834.057 kb on + strand at 1834.144 kb on + strand, within Pf1N1B4_1592 at 1834.145 kb on - strand, within Pf1N1B4_1592 at 1834.145 kb on - strand, within Pf1N1B4_1592 at 1834.180 kb on + strand, within Pf1N1B4_1592 at 1834.180 kb on + strand, within Pf1N1B4_1592 at 1834.180 kb on + strand, within Pf1N1B4_1592 at 1834.181 kb on - strand, within Pf1N1B4_1592 at 1834.266 kb on + strand, within Pf1N1B4_1592 at 1834.267 kb on - strand, within Pf1N1B4_1592 at 1834.267 kb on - strand, within Pf1N1B4_1592 at 1834.267 kb on - strand, within Pf1N1B4_1592 at 1834.312 kb on - strand at 1834.333 kb on - strand at 1834.355 kb on + strand at 1834.411 kb on + strand, within Pf1N1B4_1593 at 1834.411 kb on + strand, within Pf1N1B4_1593 at 1834.411 kb on + strand, within Pf1N1B4_1593 at 1834.412 kb on - strand, within Pf1N1B4_1593 at 1834.425 kb on - strand, within Pf1N1B4_1593 at 1834.604 kb on + strand at 1834.604 kb on + strand at 1834.624 kb on + strand at 1834.624 kb on + strand at 1834.624 kb on + strand at 1834.625 kb on - strand at 1834.625 kb on - strand at 1834.625 kb on - strand at 1834.625 kb on - strand at 1834.625 kb on - strand at 1834.669 kb on + strand at 1834.669 kb on + strand at 1834.669 kb on + strand at 1834.670 kb on - strand at 1834.670 kb on - strand at 1834.670 kb on - strand at 1834.670 kb on - strand at 1834.709 kb on + strand at 1834.741 kb on + strand at 1834.741 kb on + strand at 1834.770 kb on + strand at 1834.771 kb on - strand at 1834.798 kb on + strand at 1834.824 kb on + strand at 1834.824 kb on + strand at 1834.824 kb on + strand at 1834.824 kb on + strand at 1834.824 kb on + strand at 1834.824 kb on + strand at 1834.824 kb on + strand at 1834.824 kb on + strand at 1834.824 kb on + strand at 1834.825 kb on - strand at 1834.825 kb on - strand at 1834.825 kb on - strand at 1834.825 kb on - strand at 1834.825 kb on - strand at 1834.825 kb on - strand at 1834.825 kb on - strand at 1834.825 kb on - strand at 1834.835 kb on + strand at 1834.835 kb on + strand at 1834.836 kb on - strand at 1834.897 kb on + strand at 1834.897 kb on + strand at 1834.897 kb on + strand at 1834.898 kb on - strand at 1834.898 kb on - strand at 1834.898 kb on - strand at 1834.898 kb on - strand at 1834.898 kb on - strand at 1834.898 kb on - strand at 1835.178 kb on + strand, within Pf1N1B4_1594 at 1835.178 kb on + strand, within Pf1N1B4_1594 at 1835.178 kb on + strand, within Pf1N1B4_1594 at 1835.180 kb on + strand, within Pf1N1B4_1594 at 1835.202 kb on + strand, within Pf1N1B4_1594 at 1835.202 kb on + strand, within Pf1N1B4_1594 at 1835.203 kb on - strand, within Pf1N1B4_1594 at 1835.203 kb on - strand, within Pf1N1B4_1594 at 1835.262 kb on + strand, within Pf1N1B4_1594 at 1835.262 kb on + strand, within Pf1N1B4_1594 at 1835.263 kb on - strand, within Pf1N1B4_1594 at 1835.286 kb on + strand, within Pf1N1B4_1594 at 1835.331 kb on + strand, within Pf1N1B4_1594 at 1835.331 kb on + strand, within Pf1N1B4_1594 at 1835.331 kb on + strand, within Pf1N1B4_1594 at 1835.331 kb on + strand, within Pf1N1B4_1594 at 1835.331 kb on + strand, within Pf1N1B4_1594 at 1835.331 kb on + strand, within Pf1N1B4_1594 at 1835.332 kb on - strand, within Pf1N1B4_1594 at 1835.332 kb on - strand, within Pf1N1B4_1594 at 1835.332 kb on - strand, within Pf1N1B4_1594 at 1835.332 kb on - strand, within Pf1N1B4_1594 at 1835.363 kb on + strand, within Pf1N1B4_1594 at 1835.486 kb on + strand, within Pf1N1B4_1594 at 1835.486 kb on + strand, within Pf1N1B4_1594 at 1835.559 kb on + strand, within Pf1N1B4_1594
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 1,833,467 + Pf1N1B4_1591 0.87 -3.1 1,833,467 + Pf1N1B4_1591 0.87 +0.9 1,833,601 + -1.9 1,833,602 - +2.0 1,833,745 + +1.5 1,833,745 + +0.9 1,833,745 + -2.7 1,833,745 + -0.1 1,833,745 + +0.0 1,833,746 - +3.7 1,833,746 - -1.4 1,833,746 - +3.5 1,833,746 - -1.1 1,833,746 - +0.4 1,833,746 - -1.5 1,833,746 - +2.5 1,833,746 - -0.3 1,833,763 + -0.5 1,833,763 + +1.0 1,833,763 + +0.2 1,833,764 - +0.8 1,833,764 - +0.3 1,833,764 - -0.5 1,833,764 - +2.1 1,833,789 - -1.2 1,833,940 + Pf1N1B4_1592 0.31 +0.9 1,833,940 + Pf1N1B4_1592 0.31 -0.6 1,833,940 + Pf1N1B4_1592 0.31 -1.7 1,833,940 + Pf1N1B4_1592 0.31 -1.7 1,833,941 - Pf1N1B4_1592 0.31 -2.2 1,833,941 - Pf1N1B4_1592 0.31 -2.3 1,833,941 - Pf1N1B4_1592 0.31 -2.0 1,833,941 - Pf1N1B4_1592 0.31 -1.9 1,833,955 + Pf1N1B4_1592 0.34 +1.8 1,833,955 + Pf1N1B4_1592 0.34 +1.1 1,833,955 + Pf1N1B4_1592 0.34 +1.4 1,833,956 - Pf1N1B4_1592 0.34 -1.3 1,833,956 - Pf1N1B4_1592 0.34 +0.3 1,833,956 - Pf1N1B4_1592 0.34 +2.2 1,833,956 - Pf1N1B4_1592 0.34 +0.4 1,833,967 + Pf1N1B4_1592 0.36 +0.1 1,833,968 - Pf1N1B4_1592 0.36 -1.2 1,833,968 - Pf1N1B4_1592 0.36 -2.3 1,833,968 - Pf1N1B4_1592 0.36 -1.2 1,834,057 + -1.4 1,834,144 + Pf1N1B4_1592 0.67 -0.7 1,834,145 - Pf1N1B4_1592 0.67 -0.5 1,834,145 - Pf1N1B4_1592 0.67 +1.2 1,834,180 + Pf1N1B4_1592 0.73 +0.5 1,834,180 + Pf1N1B4_1592 0.73 -2.4 1,834,180 + Pf1N1B4_1592 0.73 +0.5 1,834,181 - Pf1N1B4_1592 0.74 +1.9 1,834,266 + Pf1N1B4_1592 0.88 -0.1 1,834,267 - Pf1N1B4_1592 0.89 -3.0 1,834,267 - Pf1N1B4_1592 0.89 -1.3 1,834,267 - Pf1N1B4_1592 0.89 -3.1 1,834,312 - +1.2 1,834,333 - -0.7 1,834,355 + +0.8 1,834,411 + Pf1N1B4_1593 0.18 +1.0 1,834,411 + Pf1N1B4_1593 0.18 -1.2 1,834,411 + Pf1N1B4_1593 0.18 -3.3 1,834,412 - Pf1N1B4_1593 0.18 -1.8 1,834,425 - Pf1N1B4_1593 0.24 -0.8 1,834,604 + +2.7 1,834,604 + -1.7 1,834,624 + +1.9 1,834,624 + +1.0 1,834,624 + -0.3 1,834,625 - +1.2 1,834,625 - +1.0 1,834,625 - -2.1 1,834,625 - +1.4 1,834,625 - -0.1 1,834,669 + -1.1 1,834,669 + +1.6 1,834,669 + -2.0 1,834,670 - -0.8 1,834,670 - +0.2 1,834,670 - -2.5 1,834,670 - -2.2 1,834,709 + +0.7 1,834,741 + -1.3 1,834,741 + +2.4 1,834,770 + -2.6 1,834,771 - -1.4 1,834,798 + +0.2 1,834,824 + +1.5 1,834,824 + +2.5 1,834,824 + -2.4 1,834,824 + -1.6 1,834,824 + -1.9 1,834,824 + -0.3 1,834,824 + +1.1 1,834,824 + +3.2 1,834,824 + +1.4 1,834,825 - -0.8 1,834,825 - -2.9 1,834,825 - +1.7 1,834,825 - -1.3 1,834,825 - -1.5 1,834,825 - -1.1 1,834,825 - -0.7 1,834,825 - -2.4 1,834,835 + +2.4 1,834,835 + +0.4 1,834,836 - -1.3 1,834,897 + -0.4 1,834,897 + -0.3 1,834,897 + -2.1 1,834,898 - -0.6 1,834,898 - -2.0 1,834,898 - -0.1 1,834,898 - -0.4 1,834,898 - +0.5 1,834,898 - -1.4 1,835,178 + Pf1N1B4_1594 0.14 -1.4 1,835,178 + Pf1N1B4_1594 0.14 -1.2 1,835,178 + Pf1N1B4_1594 0.14 -0.3 1,835,180 + Pf1N1B4_1594 0.14 +2.7 1,835,202 + Pf1N1B4_1594 0.15 -2.4 1,835,202 + Pf1N1B4_1594 0.15 -0.5 1,835,203 - Pf1N1B4_1594 0.16 -1.5 1,835,203 - Pf1N1B4_1594 0.16 -2.3 1,835,262 + Pf1N1B4_1594 0.19 +0.4 1,835,262 + Pf1N1B4_1594 0.19 -0.8 1,835,263 - Pf1N1B4_1594 0.19 -0.8 1,835,286 + Pf1N1B4_1594 0.20 -1.1 1,835,331 + Pf1N1B4_1594 0.22 -1.0 1,835,331 + Pf1N1B4_1594 0.22 +1.5 1,835,331 + Pf1N1B4_1594 0.22 -0.0 1,835,331 + Pf1N1B4_1594 0.22 +0.8 1,835,331 + Pf1N1B4_1594 0.22 -1.0 1,835,331 + Pf1N1B4_1594 0.22 -2.3 1,835,332 - Pf1N1B4_1594 0.22 +1.9 1,835,332 - Pf1N1B4_1594 0.22 +0.4 1,835,332 - Pf1N1B4_1594 0.22 -1.8 1,835,332 - Pf1N1B4_1594 0.22 +0.9 1,835,363 + Pf1N1B4_1594 0.24 +0.3 1,835,486 + Pf1N1B4_1594 0.31 -2.6 1,835,486 + Pf1N1B4_1594 0.31 +1.3 1,835,559 + Pf1N1B4_1594 0.34 -2.5
Or see this region's nucleotide sequence