Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS14510

Experiment: water survival rep B; Treatment=watercosm; Collection=outgrowth_in_CPG; Time=180_days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS14500 and RALBFv3_RS14505 overlap by 4 nucleotidesRALBFv3_RS14505 and RALBFv3_RS14510 overlap by 4 nucleotidesRALBFv3_RS14510 and RALBFv3_RS14515 are separated by 101 nucleotidesRALBFv3_RS14515 and RALBFv3_RS14520 are separated by 120 nucleotides RALBFv3_RS14500: RALBFv3_RS14500 - pyridoxine 5'-phosphate synthase, at 3,166,844 to 3,167,635 _RS14500 RALBFv3_RS14505: RALBFv3_RS14505 - holo-ACP synthase, at 3,167,632 to 3,168,075 _RS14505 RALBFv3_RS14510: RALBFv3_RS14510 - beta-N-acetylhexosaminidase, at 3,168,072 to 3,169,124 _RS14510 RALBFv3_RS14515: RALBFv3_RS14515 - elongation factor P, at 3,169,226 to 3,169,795 _RS14515 RALBFv3_RS14520: RALBFv3_RS14520 - elongation factor P maturation arginine rhamnosyltransferase EarP, at 3,169,916 to 3,171,121 _RS14520 Position (kb) 3168 3169 3170Strain fitness (log2 ratio) -7 -6 -5 -4 -3 -2 -1 0 1at 3168.820 kb on - strand, within RALBFv3_RS14510at 3169.228 kb on - strandat 3169.903 kb on - strandat 3169.905 kb on - strandat 3169.918 kb on - strandat 3169.946 kb on + strandat 3169.946 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction water survival rep B; Treatment=watercosm; Collection=outgrowth_in_CPG; Time=180_days
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3,168,820 - RALBFv3_RS14510 0.71 -7.1
3,169,228 - -0.3
3,169,903 - -0.3
3,169,905 - +0.7
3,169,918 - -1.1
3,169,946 + -1.6
3,169,946 + -2.5

Or see this region's nucleotide sequence