Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_602 and Pf1N1B4_603 are separated by 55 nucleotides Pf1N1B4_603 and Pf1N1B4_604 are separated by 77 nucleotides Pf1N1B4_604 and Pf1N1B4_605 are separated by 28 nucleotides Pf1N1B4_605 and Pf1N1B4_606 overlap by 69 nucleotides
Pf1N1B4_602 - NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12), at 695,951 to 696,952
_602
Pf1N1B4_603 - Methylglyoxal synthase (EC 4.2.3.3), at 697,008 to 697,472
_603
Pf1N1B4_604 - FIG006045: Sigma factor, ECF subfamily, at 697,550 to 698,068
_604
Pf1N1B4_605 - hypothetical protein, at 698,097 to 698,288
_605
Pf1N1B4_606 - Iron siderophore sensor protein, at 698,220 to 699,128
_606
Position (kb) 697
698 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 696.015 kb on - strand at 696.098 kb on + strand, within Pf1N1B4_602 at 696.172 kb on + strand at 696.172 kb on + strand, within Pf1N1B4_602 at 696.286 kb on + strand, within Pf1N1B4_602 at 696.287 kb on - strand at 696.313 kb on + strand, within Pf1N1B4_602 at 696.314 kb on - strand, within Pf1N1B4_602 at 696.497 kb on + strand, within Pf1N1B4_602 at 696.498 kb on - strand, within Pf1N1B4_602 at 696.950 kb on + strand at 697.088 kb on - strand at 697.389 kb on + strand, within Pf1N1B4_603 at 697.389 kb on + strand, within Pf1N1B4_603 at 697.389 kb on + strand, within Pf1N1B4_603 at 697.390 kb on - strand, within Pf1N1B4_603 at 697.390 kb on - strand, within Pf1N1B4_603 at 697.400 kb on + strand, within Pf1N1B4_603 at 697.400 kb on + strand, within Pf1N1B4_603 at 697.401 kb on - strand, within Pf1N1B4_603 at 697.401 kb on - strand, within Pf1N1B4_603 at 697.444 kb on - strand at 697.452 kb on + strand at 697.452 kb on + strand at 697.452 kb on + strand at 697.452 kb on + strand at 697.453 kb on - strand at 697.453 kb on - strand at 697.453 kb on - strand at 697.471 kb on - strand at 697.664 kb on + strand, within Pf1N1B4_604 at 697.665 kb on - strand, within Pf1N1B4_604 at 697.665 kb on - strand, within Pf1N1B4_604 at 697.958 kb on + strand, within Pf1N1B4_604 at 697.958 kb on + strand, within Pf1N1B4_604 at 697.958 kb on + strand, within Pf1N1B4_604 at 697.958 kb on + strand, within Pf1N1B4_604 at 697.959 kb on - strand, within Pf1N1B4_604 at 697.959 kb on - strand, within Pf1N1B4_604 at 697.959 kb on - strand, within Pf1N1B4_604 at 697.959 kb on - strand, within Pf1N1B4_604 at 698.098 kb on + strand at 698.098 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 696,015 - -2.7 696,098 + Pf1N1B4_602 0.15 -1.5 696,172 + -0.3 696,172 + Pf1N1B4_602 0.22 -2.2 696,286 + Pf1N1B4_602 0.33 +0.4 696,287 - -0.7 696,313 + Pf1N1B4_602 0.36 -2.8 696,314 - Pf1N1B4_602 0.36 -2.1 696,497 + Pf1N1B4_602 0.54 +0.4 696,498 - Pf1N1B4_602 0.55 -0.1 696,950 + +0.0 697,088 - +0.0 697,389 + Pf1N1B4_603 0.82 -1.0 697,389 + Pf1N1B4_603 0.82 -0.8 697,389 + Pf1N1B4_603 0.82 -2.0 697,390 - Pf1N1B4_603 0.82 -1.6 697,390 - Pf1N1B4_603 0.82 -1.2 697,400 + Pf1N1B4_603 0.84 +1.0 697,400 + Pf1N1B4_603 0.84 -1.7 697,401 - Pf1N1B4_603 0.85 -2.3 697,401 - Pf1N1B4_603 0.85 -2.4 697,444 - -0.7 697,452 + -0.2 697,452 + +0.7 697,452 + -2.4 697,452 + -2.3 697,453 - +0.4 697,453 - -0.7 697,453 - +0.2 697,471 - +0.9 697,664 + Pf1N1B4_604 0.22 -0.5 697,665 - Pf1N1B4_604 0.22 -1.8 697,665 - Pf1N1B4_604 0.22 +0.2 697,958 + Pf1N1B4_604 0.79 -0.4 697,958 + Pf1N1B4_604 0.79 -0.8 697,958 + Pf1N1B4_604 0.79 -1.0 697,958 + Pf1N1B4_604 0.79 -2.3 697,959 - Pf1N1B4_604 0.79 +0.0 697,959 - Pf1N1B4_604 0.79 +0.3 697,959 - Pf1N1B4_604 0.79 +0.6 697,959 - Pf1N1B4_604 0.79 -1.2 698,098 + +0.1 698,098 + +1.6
Or see this region's nucleotide sequence