Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4968 and Pf1N1B4_4969 are separated by 214 nucleotides Pf1N1B4_4969 and Pf1N1B4_4970 are separated by 581 nucleotides
Pf1N1B4_4968 - 4-carboxymuconolactone decarboxylase (EC 4.1.1.44), at 5,509,259 to 5,510,083
_4968
Pf1N1B4_4969 - FIG00956189: hypothetical protein, at 5,510,298 to 5,510,729
_4969
Pf1N1B4_4970 - hypothetical protein, at 5,511,311 to 5,511,718
_4970
Position (kb)
5510
5511 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5509.336 kb on - strand at 5509.336 kb on - strand at 5509.336 kb on - strand at 5509.527 kb on + strand, within Pf1N1B4_4968 at 5509.528 kb on - strand, within Pf1N1B4_4968 at 5509.547 kb on + strand, within Pf1N1B4_4968 at 5509.668 kb on - strand, within Pf1N1B4_4968 at 5509.668 kb on - strand, within Pf1N1B4_4968 at 5509.717 kb on - strand, within Pf1N1B4_4968 at 5509.717 kb on - strand, within Pf1N1B4_4968 at 5509.907 kb on - strand, within Pf1N1B4_4968 at 5509.908 kb on + strand at 5510.092 kb on + strand at 5510.092 kb on + strand at 5510.092 kb on + strand at 5510.095 kb on + strand at 5510.096 kb on - strand at 5510.096 kb on - strand at 5510.096 kb on - strand at 5510.108 kb on + strand at 5510.127 kb on - strand at 5510.285 kb on + strand at 5510.285 kb on + strand at 5510.286 kb on - strand at 5510.558 kb on - strand, within Pf1N1B4_4969 at 5510.562 kb on - strand, within Pf1N1B4_4969 at 5510.562 kb on - strand, within Pf1N1B4_4969 at 5510.681 kb on - strand, within Pf1N1B4_4969 at 5510.957 kb on + strand at 5511.058 kb on + strand at 5511.083 kb on - strand at 5511.083 kb on - strand at 5511.156 kb on + strand at 5511.156 kb on + strand at 5511.301 kb on + strand at 5511.301 kb on + strand at 5511.302 kb on - strand at 5511.321 kb on - strand at 5511.321 kb on - strand at 5511.579 kb on - strand, within Pf1N1B4_4970
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 5,509,336 - +0.0 5,509,336 - -0.3 5,509,336 - -1.7 5,509,527 + Pf1N1B4_4968 0.32 -0.1 5,509,528 - Pf1N1B4_4968 0.33 -4.0 5,509,547 + Pf1N1B4_4968 0.35 -0.3 5,509,668 - Pf1N1B4_4968 0.50 +0.1 5,509,668 - Pf1N1B4_4968 0.50 +0.1 5,509,717 - Pf1N1B4_4968 0.56 -1.0 5,509,717 - Pf1N1B4_4968 0.56 +0.3 5,509,907 - Pf1N1B4_4968 0.79 +0.0 5,509,908 + +1.1 5,510,092 + +0.5 5,510,092 + +1.1 5,510,092 + -2.4 5,510,095 + +0.8 5,510,096 - +0.3 5,510,096 - +0.5 5,510,096 - -0.3 5,510,108 + +0.8 5,510,127 - +1.6 5,510,285 + +0.1 5,510,285 + +0.1 5,510,286 - +1.3 5,510,558 - Pf1N1B4_4969 0.60 -1.0 5,510,562 - Pf1N1B4_4969 0.61 -0.7 5,510,562 - Pf1N1B4_4969 0.61 -1.5 5,510,681 - Pf1N1B4_4969 0.89 +1.1 5,510,957 + -1.0 5,511,058 + -0.1 5,511,083 - +1.9 5,511,083 - -0.7 5,511,156 + +1.0 5,511,156 + +0.1 5,511,301 + +1.2 5,511,301 + +0.1 5,511,302 - +0.3 5,511,321 - -1.9 5,511,321 - +0.0 5,511,579 - Pf1N1B4_4970 0.66 +0.1
Or see this region's nucleotide sequence