Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4428 and Pf1N1B4_4429 are separated by 17 nucleotides Pf1N1B4_4429 and Pf1N1B4_4430 overlap by 4 nucleotides Pf1N1B4_4430 and Pf1N1B4_4431 overlap by 4 nucleotides
Pf1N1B4_4428 - ABC-type polar amino acid transport system, ATPase component, at 4,888,471 to 4,889,307
_4428
Pf1N1B4_4429 - No significant database matches, at 4,889,325 to 4,889,921
_4429
Pf1N1B4_4430 - Cystathionine beta-lyase (EC 4.4.1.8), at 4,889,918 to 4,892,677
_4430
Pf1N1B4_4431 - Permeases of the major facilitator superfamily, at 4,892,674 to 4,893,861
_4431
Position (kb) 4889
4890
4891
4892
4893 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4889.305 kb on + strand at 4889.556 kb on + strand, within Pf1N1B4_4429 at 4889.557 kb on - strand, within Pf1N1B4_4429 at 4889.649 kb on + strand, within Pf1N1B4_4429 at 4889.649 kb on + strand, within Pf1N1B4_4429 at 4889.649 kb on + strand, within Pf1N1B4_4429 at 4889.877 kb on + strand at 4889.877 kb on + strand at 4889.878 kb on - strand at 4889.878 kb on - strand at 4889.914 kb on - strand at 4890.185 kb on - strand at 4890.185 kb on - strand at 4890.274 kb on - strand, within Pf1N1B4_4430 at 4890.292 kb on + strand, within Pf1N1B4_4430 at 4890.293 kb on - strand, within Pf1N1B4_4430 at 4890.555 kb on - strand, within Pf1N1B4_4430 at 4890.860 kb on + strand, within Pf1N1B4_4430 at 4890.959 kb on + strand, within Pf1N1B4_4430 at 4890.959 kb on + strand, within Pf1N1B4_4430 at 4890.959 kb on + strand, within Pf1N1B4_4430 at 4890.959 kb on + strand at 4890.960 kb on - strand, within Pf1N1B4_4430 at 4891.261 kb on + strand, within Pf1N1B4_4430 at 4891.262 kb on - strand, within Pf1N1B4_4430 at 4891.677 kb on - strand, within Pf1N1B4_4430 at 4891.757 kb on + strand, within Pf1N1B4_4430 at 4891.758 kb on - strand, within Pf1N1B4_4430 at 4891.800 kb on - strand, within Pf1N1B4_4430 at 4891.800 kb on - strand, within Pf1N1B4_4430 at 4891.961 kb on + strand, within Pf1N1B4_4430 at 4891.988 kb on + strand, within Pf1N1B4_4430 at 4891.989 kb on - strand, within Pf1N1B4_4430 at 4891.989 kb on - strand, within Pf1N1B4_4430 at 4892.043 kb on + strand, within Pf1N1B4_4430 at 4892.043 kb on + strand, within Pf1N1B4_4430 at 4892.133 kb on - strand, within Pf1N1B4_4430 at 4892.133 kb on - strand, within Pf1N1B4_4430 at 4892.296 kb on - strand, within Pf1N1B4_4430 at 4892.296 kb on - strand, within Pf1N1B4_4430 at 4892.296 kb on - strand, within Pf1N1B4_4430 at 4892.465 kb on + strand at 4892.465 kb on + strand at 4892.466 kb on - strand at 4892.466 kb on - strand at 4892.525 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 4,889,305 + -0.6 4,889,556 + Pf1N1B4_4429 0.39 +0.5 4,889,557 - Pf1N1B4_4429 0.39 +1.4 4,889,649 + Pf1N1B4_4429 0.54 +0.4 4,889,649 + Pf1N1B4_4429 0.54 +0.1 4,889,649 + Pf1N1B4_4429 0.54 -2.2 4,889,877 + -0.3 4,889,877 + +0.2 4,889,878 - +0.8 4,889,878 - +0.4 4,889,914 - +0.2 4,890,185 - +0.0 4,890,185 - -0.8 4,890,274 - Pf1N1B4_4430 0.13 -1.2 4,890,292 + Pf1N1B4_4430 0.14 -1.1 4,890,293 - Pf1N1B4_4430 0.14 -1.1 4,890,555 - Pf1N1B4_4430 0.23 +0.0 4,890,860 + Pf1N1B4_4430 0.34 -0.7 4,890,959 + Pf1N1B4_4430 0.38 -1.8 4,890,959 + Pf1N1B4_4430 0.38 +0.0 4,890,959 + Pf1N1B4_4430 0.38 +3.5 4,890,959 + +1.7 4,890,960 - Pf1N1B4_4430 0.38 -0.3 4,891,261 + Pf1N1B4_4430 0.49 -2.1 4,891,262 - Pf1N1B4_4430 0.49 +0.3 4,891,677 - Pf1N1B4_4430 0.64 +0.1 4,891,757 + Pf1N1B4_4430 0.67 -0.3 4,891,758 - Pf1N1B4_4430 0.67 -0.3 4,891,800 - Pf1N1B4_4430 0.68 -0.8 4,891,800 - Pf1N1B4_4430 0.68 -1.1 4,891,961 + Pf1N1B4_4430 0.74 -1.1 4,891,988 + Pf1N1B4_4430 0.75 +0.6 4,891,989 - Pf1N1B4_4430 0.75 -0.4 4,891,989 - Pf1N1B4_4430 0.75 -0.3 4,892,043 + Pf1N1B4_4430 0.77 +0.9 4,892,043 + Pf1N1B4_4430 0.77 -0.1 4,892,133 - Pf1N1B4_4430 0.80 -2.0 4,892,133 - Pf1N1B4_4430 0.80 +0.7 4,892,296 - Pf1N1B4_4430 0.86 -0.2 4,892,296 - Pf1N1B4_4430 0.86 -0.8 4,892,296 - Pf1N1B4_4430 0.86 -2.9 4,892,465 + -2.0 4,892,465 + +1.4 4,892,466 - +0.7 4,892,466 - -0.3 4,892,525 + -0.5
Or see this region's nucleotide sequence