Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3963

Experiment: nitrogen source nitrite 0.5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3962 and Pf1N1B4_3963 are separated by 10 nucleotidesPf1N1B4_3963 and Pf1N1B4_3964 are separated by 2 nucleotides Pf1N1B4_3962 - Glycerol-3-phosphate transporter, at 4,401,282 to 4,402,607 _3962 Pf1N1B4_3963 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49), at 4,402,618 to 4,404,009 _3963 Pf1N1B4_3964 - VgrG protein, at 4,404,012 to 4,405,436 _3964 Position (kb) 4402 4403 4404 4405Strain fitness (log2 ratio) -2 -1 0 1 2at 4401.727 kb on - strand, within Pf1N1B4_3962at 4401.774 kb on + strand, within Pf1N1B4_3962at 4401.775 kb on - strand, within Pf1N1B4_3962at 4401.776 kb on + strand, within Pf1N1B4_3962at 4401.863 kb on + strand, within Pf1N1B4_3962at 4401.863 kb on + strand, within Pf1N1B4_3962at 4401.863 kb on + strand, within Pf1N1B4_3962at 4402.011 kb on + strand, within Pf1N1B4_3962at 4402.012 kb on - strand, within Pf1N1B4_3962at 4402.012 kb on - strand, within Pf1N1B4_3962at 4402.588 kb on + strandat 4402.601 kb on + strandat 4402.601 kb on + strandat 4402.601 kb on + strandat 4402.602 kb on - strandat 4402.602 kb on - strandat 4402.602 kb on - strandat 4402.602 kb on - strandat 4402.602 kb on - strandat 4402.605 kb on + strandat 4402.605 kb on + strandat 4402.605 kb on + strandat 4402.605 kb on + strandat 4402.605 kb on + strandat 4402.606 kb on - strandat 4402.606 kb on - strandat 4402.859 kb on - strandat 4402.859 kb on - strandat 4402.859 kb on - strand, within Pf1N1B4_3963at 4402.957 kb on + strand, within Pf1N1B4_3963at 4402.958 kb on - strand, within Pf1N1B4_3963at 4402.996 kb on + strand, within Pf1N1B4_3963at 4402.996 kb on + strand, within Pf1N1B4_3963at 4402.997 kb on - strand, within Pf1N1B4_3963at 4402.997 kb on - strand, within Pf1N1B4_3963at 4403.099 kb on + strand, within Pf1N1B4_3963at 4403.099 kb on + strand, within Pf1N1B4_3963at 4403.100 kb on - strand, within Pf1N1B4_3963at 4403.100 kb on - strand, within Pf1N1B4_3963at 4403.100 kb on - strand, within Pf1N1B4_3963at 4403.107 kb on - strand, within Pf1N1B4_3963at 4403.252 kb on + strand, within Pf1N1B4_3963at 4403.253 kb on - strand, within Pf1N1B4_3963at 4403.476 kb on + strand, within Pf1N1B4_3963at 4403.476 kb on + strand, within Pf1N1B4_3963at 4403.476 kb on + strand, within Pf1N1B4_3963at 4403.476 kb on + strand, within Pf1N1B4_3963at 4403.477 kb on - strand, within Pf1N1B4_3963at 4403.526 kb on + strand, within Pf1N1B4_3963at 4403.646 kb on + strandat 4403.684 kb on + strand, within Pf1N1B4_3963at 4403.725 kb on + strand, within Pf1N1B4_3963at 4403.876 kb on - strandat 4404.054 kb on + strandat 4404.355 kb on + strand, within Pf1N1B4_3964at 4404.434 kb on + strandat 4404.435 kb on - strand, within Pf1N1B4_3964at 4404.435 kb on - strand, within Pf1N1B4_3964at 4404.435 kb on - strand, within Pf1N1B4_3964at 4404.435 kb on - strand, within Pf1N1B4_3964at 4404.435 kb on - strand, within Pf1N1B4_3964at 4404.436 kb on + strandat 4404.437 kb on - strand, within Pf1N1B4_3964at 4404.907 kb on - strand, within Pf1N1B4_3964at 4404.907 kb on - strand, within Pf1N1B4_3964at 4404.938 kb on - strandat 4404.994 kb on + strand, within Pf1N1B4_3964at 4404.994 kb on + strand, within Pf1N1B4_3964at 4404.995 kb on - strand, within Pf1N1B4_3964

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM
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4,401,727 - Pf1N1B4_3962 0.34 -2.4
4,401,774 + Pf1N1B4_3962 0.37 +0.4
4,401,775 - Pf1N1B4_3962 0.37 +0.6
4,401,776 + Pf1N1B4_3962 0.37 -0.2
4,401,863 + Pf1N1B4_3962 0.44 +0.3
4,401,863 + Pf1N1B4_3962 0.44 -0.1
4,401,863 + Pf1N1B4_3962 0.44 +0.4
4,402,011 + Pf1N1B4_3962 0.55 -0.3
4,402,012 - Pf1N1B4_3962 0.55 +1.8
4,402,012 - Pf1N1B4_3962 0.55 +0.4
4,402,588 + +1.4
4,402,601 + +0.3
4,402,601 + -1.9
4,402,601 + -0.0
4,402,602 - -0.2
4,402,602 - +1.2
4,402,602 - +0.2
4,402,602 - -0.2
4,402,602 - -0.0
4,402,605 + +0.8
4,402,605 + +0.1
4,402,605 + -0.7
4,402,605 + +0.8
4,402,605 + -0.1
4,402,606 - +1.3
4,402,606 - +0.3
4,402,859 - +1.7
4,402,859 - +0.1
4,402,859 - Pf1N1B4_3963 0.17 -0.2
4,402,957 + Pf1N1B4_3963 0.24 -1.9
4,402,958 - Pf1N1B4_3963 0.24 +0.6
4,402,996 + Pf1N1B4_3963 0.27 -0.3
4,402,996 + Pf1N1B4_3963 0.27 -2.5
4,402,997 - Pf1N1B4_3963 0.27 +1.9
4,402,997 - Pf1N1B4_3963 0.27 -0.7
4,403,099 + Pf1N1B4_3963 0.35 -0.3
4,403,099 + Pf1N1B4_3963 0.35 +0.1
4,403,100 - Pf1N1B4_3963 0.35 +1.8
4,403,100 - Pf1N1B4_3963 0.35 +0.5
4,403,100 - Pf1N1B4_3963 0.35 +0.1
4,403,107 - Pf1N1B4_3963 0.35 +0.1
4,403,252 + Pf1N1B4_3963 0.46 -0.7
4,403,253 - Pf1N1B4_3963 0.46 +0.3
4,403,476 + Pf1N1B4_3963 0.62 +1.7
4,403,476 + Pf1N1B4_3963 0.62 -0.5
4,403,476 + Pf1N1B4_3963 0.62 -0.4
4,403,476 + Pf1N1B4_3963 0.62 -1.6
4,403,477 - Pf1N1B4_3963 0.62 -0.1
4,403,526 + Pf1N1B4_3963 0.65 -0.8
4,403,646 + -1.7
4,403,684 + Pf1N1B4_3963 0.77 -1.2
4,403,725 + Pf1N1B4_3963 0.80 -1.2
4,403,876 - +0.8
4,404,054 + -0.6
4,404,355 + Pf1N1B4_3964 0.24 +2.3
4,404,434 + -1.6
4,404,435 - Pf1N1B4_3964 0.30 +1.2
4,404,435 - Pf1N1B4_3964 0.30 -0.1
4,404,435 - Pf1N1B4_3964 0.30 +1.4
4,404,435 - Pf1N1B4_3964 0.30 +0.8
4,404,435 - Pf1N1B4_3964 0.30 -0.2
4,404,436 + -2.6
4,404,437 - Pf1N1B4_3964 0.30 +0.7
4,404,907 - Pf1N1B4_3964 0.63 -0.3
4,404,907 - Pf1N1B4_3964 0.63 -0.9
4,404,938 - +0.8
4,404,994 + Pf1N1B4_3964 0.69 +1.1
4,404,994 + Pf1N1B4_3964 0.69 -1.3
4,404,995 - Pf1N1B4_3964 0.69 +0.1

Or see this region's nucleotide sequence