Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3962 and Pf1N1B4_3963 are separated by 10 nucleotides Pf1N1B4_3963 and Pf1N1B4_3964 are separated by 2 nucleotides
Pf1N1B4_3962 - Glycerol-3-phosphate transporter, at 4,401,282 to 4,402,607
_3962
Pf1N1B4_3963 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49), at 4,402,618 to 4,404,009
_3963
Pf1N1B4_3964 - VgrG protein, at 4,404,012 to 4,405,436
_3964
Position (kb)
4402
4403
4404
4405 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4401.727 kb on - strand, within Pf1N1B4_3962 at 4401.774 kb on + strand, within Pf1N1B4_3962 at 4401.775 kb on - strand, within Pf1N1B4_3962 at 4401.776 kb on + strand, within Pf1N1B4_3962 at 4401.863 kb on + strand, within Pf1N1B4_3962 at 4401.863 kb on + strand, within Pf1N1B4_3962 at 4401.863 kb on + strand, within Pf1N1B4_3962 at 4402.011 kb on + strand, within Pf1N1B4_3962 at 4402.012 kb on - strand, within Pf1N1B4_3962 at 4402.012 kb on - strand, within Pf1N1B4_3962 at 4402.588 kb on + strand at 4402.601 kb on + strand at 4402.601 kb on + strand at 4402.601 kb on + strand at 4402.602 kb on - strand at 4402.602 kb on - strand at 4402.602 kb on - strand at 4402.602 kb on - strand at 4402.602 kb on - strand at 4402.605 kb on + strand at 4402.605 kb on + strand at 4402.605 kb on + strand at 4402.605 kb on + strand at 4402.605 kb on + strand at 4402.606 kb on - strand at 4402.606 kb on - strand at 4402.859 kb on - strand at 4402.859 kb on - strand at 4402.859 kb on - strand, within Pf1N1B4_3963 at 4402.957 kb on + strand, within Pf1N1B4_3963 at 4402.958 kb on - strand, within Pf1N1B4_3963 at 4402.996 kb on + strand, within Pf1N1B4_3963 at 4402.996 kb on + strand, within Pf1N1B4_3963 at 4402.997 kb on - strand, within Pf1N1B4_3963 at 4402.997 kb on - strand, within Pf1N1B4_3963 at 4403.099 kb on + strand, within Pf1N1B4_3963 at 4403.099 kb on + strand, within Pf1N1B4_3963 at 4403.100 kb on - strand, within Pf1N1B4_3963 at 4403.100 kb on - strand, within Pf1N1B4_3963 at 4403.100 kb on - strand, within Pf1N1B4_3963 at 4403.107 kb on - strand, within Pf1N1B4_3963 at 4403.252 kb on + strand, within Pf1N1B4_3963 at 4403.253 kb on - strand, within Pf1N1B4_3963 at 4403.476 kb on + strand, within Pf1N1B4_3963 at 4403.476 kb on + strand, within Pf1N1B4_3963 at 4403.476 kb on + strand, within Pf1N1B4_3963 at 4403.476 kb on + strand, within Pf1N1B4_3963 at 4403.477 kb on - strand, within Pf1N1B4_3963 at 4403.526 kb on + strand, within Pf1N1B4_3963 at 4403.646 kb on + strand at 4403.684 kb on + strand, within Pf1N1B4_3963 at 4403.725 kb on + strand, within Pf1N1B4_3963 at 4403.876 kb on - strand at 4404.054 kb on + strand at 4404.355 kb on + strand, within Pf1N1B4_3964 at 4404.434 kb on + strand at 4404.435 kb on - strand, within Pf1N1B4_3964 at 4404.435 kb on - strand, within Pf1N1B4_3964 at 4404.435 kb on - strand, within Pf1N1B4_3964 at 4404.435 kb on - strand, within Pf1N1B4_3964 at 4404.435 kb on - strand, within Pf1N1B4_3964 at 4404.436 kb on + strand at 4404.437 kb on - strand, within Pf1N1B4_3964 at 4404.907 kb on - strand, within Pf1N1B4_3964 at 4404.907 kb on - strand, within Pf1N1B4_3964 at 4404.938 kb on - strand at 4404.994 kb on + strand, within Pf1N1B4_3964 at 4404.994 kb on + strand, within Pf1N1B4_3964 at 4404.995 kb on - strand, within Pf1N1B4_3964
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 4,401,727 - Pf1N1B4_3962 0.34 -2.4 4,401,774 + Pf1N1B4_3962 0.37 +0.4 4,401,775 - Pf1N1B4_3962 0.37 +0.6 4,401,776 + Pf1N1B4_3962 0.37 -0.2 4,401,863 + Pf1N1B4_3962 0.44 +0.3 4,401,863 + Pf1N1B4_3962 0.44 -0.1 4,401,863 + Pf1N1B4_3962 0.44 +0.4 4,402,011 + Pf1N1B4_3962 0.55 -0.3 4,402,012 - Pf1N1B4_3962 0.55 +1.8 4,402,012 - Pf1N1B4_3962 0.55 +0.4 4,402,588 + +1.4 4,402,601 + +0.3 4,402,601 + -1.9 4,402,601 + -0.0 4,402,602 - -0.2 4,402,602 - +1.2 4,402,602 - +0.2 4,402,602 - -0.2 4,402,602 - -0.0 4,402,605 + +0.8 4,402,605 + +0.1 4,402,605 + -0.7 4,402,605 + +0.8 4,402,605 + -0.1 4,402,606 - +1.3 4,402,606 - +0.3 4,402,859 - +1.7 4,402,859 - +0.1 4,402,859 - Pf1N1B4_3963 0.17 -0.2 4,402,957 + Pf1N1B4_3963 0.24 -1.9 4,402,958 - Pf1N1B4_3963 0.24 +0.6 4,402,996 + Pf1N1B4_3963 0.27 -0.3 4,402,996 + Pf1N1B4_3963 0.27 -2.5 4,402,997 - Pf1N1B4_3963 0.27 +1.9 4,402,997 - Pf1N1B4_3963 0.27 -0.7 4,403,099 + Pf1N1B4_3963 0.35 -0.3 4,403,099 + Pf1N1B4_3963 0.35 +0.1 4,403,100 - Pf1N1B4_3963 0.35 +1.8 4,403,100 - Pf1N1B4_3963 0.35 +0.5 4,403,100 - Pf1N1B4_3963 0.35 +0.1 4,403,107 - Pf1N1B4_3963 0.35 +0.1 4,403,252 + Pf1N1B4_3963 0.46 -0.7 4,403,253 - Pf1N1B4_3963 0.46 +0.3 4,403,476 + Pf1N1B4_3963 0.62 +1.7 4,403,476 + Pf1N1B4_3963 0.62 -0.5 4,403,476 + Pf1N1B4_3963 0.62 -0.4 4,403,476 + Pf1N1B4_3963 0.62 -1.6 4,403,477 - Pf1N1B4_3963 0.62 -0.1 4,403,526 + Pf1N1B4_3963 0.65 -0.8 4,403,646 + -1.7 4,403,684 + Pf1N1B4_3963 0.77 -1.2 4,403,725 + Pf1N1B4_3963 0.80 -1.2 4,403,876 - +0.8 4,404,054 + -0.6 4,404,355 + Pf1N1B4_3964 0.24 +2.3 4,404,434 + -1.6 4,404,435 - Pf1N1B4_3964 0.30 +1.2 4,404,435 - Pf1N1B4_3964 0.30 -0.1 4,404,435 - Pf1N1B4_3964 0.30 +1.4 4,404,435 - Pf1N1B4_3964 0.30 +0.8 4,404,435 - Pf1N1B4_3964 0.30 -0.2 4,404,436 + -2.6 4,404,437 - Pf1N1B4_3964 0.30 +0.7 4,404,907 - Pf1N1B4_3964 0.63 -0.3 4,404,907 - Pf1N1B4_3964 0.63 -0.9 4,404,938 - +0.8 4,404,994 + Pf1N1B4_3964 0.69 +1.1 4,404,994 + Pf1N1B4_3964 0.69 -1.3 4,404,995 - Pf1N1B4_3964 0.69 +0.1
Or see this region's nucleotide sequence