Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3516 and Pf1N1B4_3517 are separated by 61 nucleotides Pf1N1B4_3517 and Pf1N1B4_3518 are separated by 15 nucleotides Pf1N1B4_3518 and Pf1N1B4_3519 are separated by 31 nucleotides Pf1N1B4_3519 and Pf1N1B4_3520 overlap by 4 nucleotides
Pf1N1B4_3516 - TonB-dependent receptor; Outer membrane receptor for ferrienterochelin and colicins, at 3,923,424 to 3,925,547
_3516
Pf1N1B4_3517 - hypothetical protein, at 3,925,609 to 3,925,827
_3517
Pf1N1B4_3518 - Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94), at 3,925,843 to 3,926,769
_3518
Pf1N1B4_3519 - Lipase (EC 3.1.1.3), at 3,926,801 to 3,927,148
_3519
Pf1N1B4_3520 - Phosphohistidine phosphatase SixA, at 3,927,145 to 3,927,597
_3520
Position (kb)
3925
3926
3927 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3924.995 kb on - strand, within Pf1N1B4_3516 at 3925.007 kb on + strand, within Pf1N1B4_3516 at 3925.169 kb on + strand, within Pf1N1B4_3516 at 3925.205 kb on - strand, within Pf1N1B4_3516 at 3925.267 kb on + strand, within Pf1N1B4_3516 at 3925.267 kb on + strand, within Pf1N1B4_3516 at 3925.267 kb on + strand, within Pf1N1B4_3516 at 3925.267 kb on + strand, within Pf1N1B4_3516 at 3925.267 kb on + strand, within Pf1N1B4_3516 at 3925.267 kb on + strand, within Pf1N1B4_3516 at 3925.268 kb on - strand at 3925.268 kb on - strand, within Pf1N1B4_3516 at 3925.268 kb on - strand, within Pf1N1B4_3516 at 3925.557 kb on + strand at 3925.557 kb on + strand at 3925.558 kb on - strand at 3925.558 kb on - strand at 3925.651 kb on + strand at 3925.663 kb on + strand, within Pf1N1B4_3517 at 3925.701 kb on + strand, within Pf1N1B4_3517 at 3925.701 kb on + strand, within Pf1N1B4_3517 at 3925.743 kb on + strand, within Pf1N1B4_3517 at 3926.606 kb on + strand, within Pf1N1B4_3518 at 3926.607 kb on - strand, within Pf1N1B4_3518 at 3926.638 kb on + strand, within Pf1N1B4_3518 at 3926.638 kb on + strand, within Pf1N1B4_3518 at 3926.639 kb on - strand, within Pf1N1B4_3518 at 3926.639 kb on - strand, within Pf1N1B4_3518 at 3926.639 kb on - strand, within Pf1N1B4_3518 at 3926.639 kb on - strand, within Pf1N1B4_3518 at 3926.639 kb on - strand, within Pf1N1B4_3518 at 3926.708 kb on + strand at 3926.745 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.768 kb on - strand at 3926.768 kb on - strand at 3926.771 kb on + strand at 3926.781 kb on + strand at 3926.781 kb on + strand at 3926.781 kb on + strand at 3926.781 kb on + strand at 3926.781 kb on + strand at 3926.782 kb on - strand at 3926.782 kb on - strand at 3926.782 kb on - strand at 3926.782 kb on - strand at 3926.828 kb on + strand at 3926.828 kb on + strand at 3926.828 kb on + strand at 3926.828 kb on + strand at 3926.867 kb on + strand, within Pf1N1B4_3519 at 3926.867 kb on + strand, within Pf1N1B4_3519 at 3926.867 kb on + strand at 3926.919 kb on + strand, within Pf1N1B4_3519 at 3926.919 kb on + strand, within Pf1N1B4_3519 at 3926.919 kb on + strand, within Pf1N1B4_3519 at 3926.919 kb on + strand, within Pf1N1B4_3519 at 3926.920 kb on - strand, within Pf1N1B4_3519 at 3926.931 kb on - strand, within Pf1N1B4_3519 at 3926.942 kb on + strand, within Pf1N1B4_3519 at 3927.144 kb on + strand at 3927.159 kb on - strand at 3927.286 kb on + strand, within Pf1N1B4_3520 at 3927.286 kb on + strand, within Pf1N1B4_3520 at 3927.286 kb on + strand, within Pf1N1B4_3520 at 3927.286 kb on + strand, within Pf1N1B4_3520 at 3927.286 kb on + strand, within Pf1N1B4_3520 at 3927.286 kb on + strand, within Pf1N1B4_3520 at 3927.287 kb on - strand, within Pf1N1B4_3520 at 3927.419 kb on + strand, within Pf1N1B4_3520 at 3927.478 kb on + strand, within Pf1N1B4_3520 at 3927.479 kb on - strand, within Pf1N1B4_3520 at 3927.479 kb on - strand, within Pf1N1B4_3520 at 3927.479 kb on - strand, within Pf1N1B4_3520 at 3927.639 kb on + strand at 3927.640 kb on - strand at 3927.727 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 3,924,995 - Pf1N1B4_3516 0.74 +0.5 3,925,007 + Pf1N1B4_3516 0.75 +0.5 3,925,169 + Pf1N1B4_3516 0.82 -0.6 3,925,205 - Pf1N1B4_3516 0.84 -0.3 3,925,267 + Pf1N1B4_3516 0.87 -2.3 3,925,267 + Pf1N1B4_3516 0.87 -2.3 3,925,267 + Pf1N1B4_3516 0.87 -0.3 3,925,267 + Pf1N1B4_3516 0.87 -1.1 3,925,267 + Pf1N1B4_3516 0.87 -1.3 3,925,267 + Pf1N1B4_3516 0.87 +0.6 3,925,268 - -0.7 3,925,268 - Pf1N1B4_3516 0.87 +0.0 3,925,268 - Pf1N1B4_3516 0.87 +0.8 3,925,557 + -0.1 3,925,557 + +1.7 3,925,558 - +0.2 3,925,558 - -0.1 3,925,651 + -1.0 3,925,663 + Pf1N1B4_3517 0.25 -0.0 3,925,701 + Pf1N1B4_3517 0.42 +0.1 3,925,701 + Pf1N1B4_3517 0.42 +1.3 3,925,743 + Pf1N1B4_3517 0.61 +0.4 3,926,606 + Pf1N1B4_3518 0.82 -2.2 3,926,607 - Pf1N1B4_3518 0.82 -1.8 3,926,638 + Pf1N1B4_3518 0.86 -1.5 3,926,638 + Pf1N1B4_3518 0.86 -3.1 3,926,639 - Pf1N1B4_3518 0.86 -1.9 3,926,639 - Pf1N1B4_3518 0.86 -0.0 3,926,639 - Pf1N1B4_3518 0.86 -1.0 3,926,639 - Pf1N1B4_3518 0.86 -2.0 3,926,639 - Pf1N1B4_3518 0.86 +0.7 3,926,708 + -2.1 3,926,745 + -0.4 3,926,767 + +0.6 3,926,767 + -0.7 3,926,767 + -0.2 3,926,767 + -0.6 3,926,767 + -2.5 3,926,767 + -2.6 3,926,767 + -1.1 3,926,767 + +2.4 3,926,767 + +0.1 3,926,768 - +0.5 3,926,768 - -1.1 3,926,771 + -0.7 3,926,781 + +0.0 3,926,781 + -0.6 3,926,781 + +1.0 3,926,781 + -0.9 3,926,781 + -0.2 3,926,782 - +0.4 3,926,782 - -0.6 3,926,782 - -1.3 3,926,782 - -0.1 3,926,828 + +0.6 3,926,828 + +0.4 3,926,828 + +2.3 3,926,828 + +0.8 3,926,867 + Pf1N1B4_3519 0.19 +0.5 3,926,867 + Pf1N1B4_3519 0.19 -1.0 3,926,867 + -1.3 3,926,919 + Pf1N1B4_3519 0.34 -2.5 3,926,919 + Pf1N1B4_3519 0.34 -0.6 3,926,919 + Pf1N1B4_3519 0.34 -1.3 3,926,919 + Pf1N1B4_3519 0.34 +0.2 3,926,920 - Pf1N1B4_3519 0.34 -0.1 3,926,931 - Pf1N1B4_3519 0.37 -1.3 3,926,942 + Pf1N1B4_3519 0.41 -0.6 3,927,144 + -1.6 3,927,159 - -0.0 3,927,286 + Pf1N1B4_3520 0.31 -0.1 3,927,286 + Pf1N1B4_3520 0.31 -0.4 3,927,286 + Pf1N1B4_3520 0.31 +1.0 3,927,286 + Pf1N1B4_3520 0.31 -1.8 3,927,286 + Pf1N1B4_3520 0.31 +0.2 3,927,286 + Pf1N1B4_3520 0.31 +0.5 3,927,287 - Pf1N1B4_3520 0.31 -0.7 3,927,419 + Pf1N1B4_3520 0.60 -0.4 3,927,478 + Pf1N1B4_3520 0.74 +0.9 3,927,479 - Pf1N1B4_3520 0.74 +0.6 3,927,479 - Pf1N1B4_3520 0.74 -0.6 3,927,479 - Pf1N1B4_3520 0.74 -0.4 3,927,639 + +1.7 3,927,640 - -1.5 3,927,727 - -2.7
Or see this region's nucleotide sequence