Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3141 and Pf1N1B4_3142 are separated by 80 nucleotides Pf1N1B4_3142 and Pf1N1B4_3143 are separated by 83 nucleotides Pf1N1B4_3143 and Pf1N1B4_3144 are separated by 53 nucleotides Pf1N1B4_3144 and Pf1N1B4_3145 are separated by 43 nucleotides
Pf1N1B4_3141 - FIG00959768: hypothetical protein, at 3,524,567 to 3,525,085
_3141
Pf1N1B4_3142 - C-terminal domain of CinA type S; Protein Implicated in DNA repair function with RecA and MutS, at 3,525,166 to 3,525,666
_3142
Pf1N1B4_3143 - RecA protein, at 3,525,750 to 3,526,802
_3143
Pf1N1B4_3144 - Regulatory protein RecX, at 3,526,856 to 3,527,278
_3144
Pf1N1B4_3145 - Decarboxylase family protein, at 3,527,322 to 3,528,440
_3145
Position (kb)
3525
3526
3527 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3524.818 kb on + strand, within Pf1N1B4_3141 at 3524.819 kb on - strand, within Pf1N1B4_3141 at 3525.005 kb on - strand at 3525.005 kb on - strand at 3525.062 kb on + strand at 3525.062 kb on + strand at 3525.062 kb on + strand at 3525.062 kb on + strand at 3525.063 kb on - strand at 3525.063 kb on - strand at 3525.063 kb on - strand at 3525.063 kb on - strand at 3525.130 kb on - strand at 3525.174 kb on + strand at 3525.174 kb on + strand at 3525.174 kb on + strand at 3525.316 kb on + strand, within Pf1N1B4_3142 at 3525.317 kb on - strand, within Pf1N1B4_3142 at 3525.325 kb on + strand, within Pf1N1B4_3142 at 3525.325 kb on + strand, within Pf1N1B4_3142 at 3525.325 kb on + strand at 3525.326 kb on - strand, within Pf1N1B4_3142 at 3525.326 kb on - strand, within Pf1N1B4_3142 at 3525.326 kb on - strand, within Pf1N1B4_3142 at 3525.473 kb on + strand, within Pf1N1B4_3142 at 3525.474 kb on - strand, within Pf1N1B4_3142 at 3525.565 kb on + strand, within Pf1N1B4_3142 at 3525.565 kb on + strand, within Pf1N1B4_3142 at 3525.565 kb on + strand, within Pf1N1B4_3142 at 3525.728 kb on + strand at 3525.729 kb on - strand at 3525.735 kb on + strand at 3525.735 kb on + strand at 3525.735 kb on + strand at 3525.735 kb on + strand at 3525.939 kb on + strand, within Pf1N1B4_3143 at 3525.939 kb on + strand, within Pf1N1B4_3143 at 3526.398 kb on + strand, within Pf1N1B4_3143 at 3526.398 kb on + strand, within Pf1N1B4_3143 at 3526.551 kb on + strand, within Pf1N1B4_3143 at 3526.551 kb on + strand, within Pf1N1B4_3143 at 3526.552 kb on - strand, within Pf1N1B4_3143 at 3526.617 kb on + strand, within Pf1N1B4_3143 at 3526.617 kb on + strand, within Pf1N1B4_3143 at 3526.822 kb on - strand at 3527.169 kb on + strand, within Pf1N1B4_3144 at 3527.292 kb on + strand at 3527.293 kb on - strand at 3527.293 kb on - strand at 3527.401 kb on + strand at 3527.401 kb on + strand at 3527.401 kb on + strand at 3527.402 kb on - strand at 3527.409 kb on + strand at 3527.410 kb on - strand at 3527.410 kb on - strand at 3527.410 kb on - strand at 3527.410 kb on - strand at 3527.504 kb on - strand, within Pf1N1B4_3145 at 3527.644 kb on + strand, within Pf1N1B4_3145 at 3527.683 kb on + strand, within Pf1N1B4_3145 at 3527.684 kb on - strand at 3527.684 kb on - strand, within Pf1N1B4_3145 at 3527.796 kb on + strand, within Pf1N1B4_3145 at 3527.796 kb on + strand, within Pf1N1B4_3145
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 3,524,818 + Pf1N1B4_3141 0.48 +1.1 3,524,819 - Pf1N1B4_3141 0.49 -0.6 3,525,005 - -0.1 3,525,005 - +0.5 3,525,062 + -0.4 3,525,062 + +0.4 3,525,062 + -0.5 3,525,062 + -1.1 3,525,063 - -1.2 3,525,063 - +0.9 3,525,063 - -0.0 3,525,063 - +0.7 3,525,130 - -0.9 3,525,174 + -2.1 3,525,174 + +1.7 3,525,174 + -1.5 3,525,316 + Pf1N1B4_3142 0.30 +0.4 3,525,317 - Pf1N1B4_3142 0.30 -1.7 3,525,325 + Pf1N1B4_3142 0.32 -1.2 3,525,325 + Pf1N1B4_3142 0.32 -1.8 3,525,325 + +0.1 3,525,326 - Pf1N1B4_3142 0.32 +0.4 3,525,326 - Pf1N1B4_3142 0.32 +1.1 3,525,326 - Pf1N1B4_3142 0.32 -0.2 3,525,473 + Pf1N1B4_3142 0.61 -1.4 3,525,474 - Pf1N1B4_3142 0.61 -0.8 3,525,565 + Pf1N1B4_3142 0.80 +0.2 3,525,565 + Pf1N1B4_3142 0.80 +0.3 3,525,565 + Pf1N1B4_3142 0.80 -1.8 3,525,728 + -0.1 3,525,729 - -0.2 3,525,735 + -0.8 3,525,735 + +1.3 3,525,735 + -0.9 3,525,735 + +0.1 3,525,939 + Pf1N1B4_3143 0.18 +1.7 3,525,939 + Pf1N1B4_3143 0.18 +0.2 3,526,398 + Pf1N1B4_3143 0.62 -1.2 3,526,398 + Pf1N1B4_3143 0.62 -0.5 3,526,551 + Pf1N1B4_3143 0.76 -3.9 3,526,551 + Pf1N1B4_3143 0.76 -2.2 3,526,552 - Pf1N1B4_3143 0.76 -1.7 3,526,617 + Pf1N1B4_3143 0.82 -2.9 3,526,617 + Pf1N1B4_3143 0.82 -1.8 3,526,822 - -1.6 3,527,169 + Pf1N1B4_3144 0.74 -1.5 3,527,292 + -2.4 3,527,293 - +0.7 3,527,293 - +1.4 3,527,401 + +1.1 3,527,401 + +0.4 3,527,401 + +0.4 3,527,402 - +1.8 3,527,409 + +0.6 3,527,410 - +1.4 3,527,410 - -0.2 3,527,410 - -1.4 3,527,410 - -0.4 3,527,504 - Pf1N1B4_3145 0.16 -0.3 3,527,644 + Pf1N1B4_3145 0.29 -0.2 3,527,683 + Pf1N1B4_3145 0.32 -0.6 3,527,684 - -0.7 3,527,684 - Pf1N1B4_3145 0.32 -1.4 3,527,796 + Pf1N1B4_3145 0.42 -0.2 3,527,796 + Pf1N1B4_3145 0.42 -1.8
Or see this region's nucleotide sequence