Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2445

Experiment: nitrogen source nitrite 0.5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2444 and Pf1N1B4_2445 are separated by 121 nucleotidesPf1N1B4_2445 and Pf1N1B4_2446 are separated by 26 nucleotides Pf1N1B4_2444 - Cholesterol oxidase (EC 1.1.3.6), at 2,770,004 to 2,773,462 _2444 Pf1N1B4_2445 - FIG00506946: hypothetical protein, at 2,773,584 to 2,775,452 _2445 Pf1N1B4_2446 - miscellaneous; unknown, at 2,775,479 to 2,776,729 _2446 Position (kb) 2773 2774 2775 2776Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 2772.617 kb on + strand, within Pf1N1B4_2444at 2772.617 kb on + strand, within Pf1N1B4_2444at 2772.618 kb on - strand, within Pf1N1B4_2444at 2773.029 kb on - strand, within Pf1N1B4_2444at 2773.067 kb on + strand, within Pf1N1B4_2444at 2773.217 kb on + strandat 2773.218 kb on - strandat 2773.218 kb on - strandat 2773.218 kb on - strandat 2773.218 kb on - strandat 2773.218 kb on - strandat 2773.218 kb on - strandat 2773.310 kb on + strandat 2773.311 kb on - strandat 2773.593 kb on + strandat 2773.593 kb on + strandat 2773.593 kb on + strandat 2773.593 kb on + strandat 2773.593 kb on + strandat 2773.594 kb on - strandat 2773.594 kb on - strandat 2773.767 kb on + strandat 2773.768 kb on - strandat 2773.768 kb on - strandat 2773.797 kb on + strand, within Pf1N1B4_2445at 2773.797 kb on + strand, within Pf1N1B4_2445at 2773.797 kb on + strand, within Pf1N1B4_2445at 2773.797 kb on + strand, within Pf1N1B4_2445at 2773.797 kb on + strand, within Pf1N1B4_2445at 2773.797 kb on + strand, within Pf1N1B4_2445at 2773.798 kb on - strand, within Pf1N1B4_2445at 2773.798 kb on - strand, within Pf1N1B4_2445at 2773.798 kb on - strand, within Pf1N1B4_2445at 2773.947 kb on + strand, within Pf1N1B4_2445at 2773.947 kb on + strand, within Pf1N1B4_2445at 2774.139 kb on + strand, within Pf1N1B4_2445at 2774.140 kb on - strand, within Pf1N1B4_2445at 2774.140 kb on - strand, within Pf1N1B4_2445at 2774.160 kb on + strand, within Pf1N1B4_2445at 2774.160 kb on + strand, within Pf1N1B4_2445at 2774.161 kb on - strand, within Pf1N1B4_2445at 2774.166 kb on + strand, within Pf1N1B4_2445at 2774.167 kb on - strand, within Pf1N1B4_2445at 2774.206 kb on - strand, within Pf1N1B4_2445at 2774.208 kb on + strand, within Pf1N1B4_2445at 2774.208 kb on + strand, within Pf1N1B4_2445at 2774.208 kb on + strand, within Pf1N1B4_2445at 2774.209 kb on - strand, within Pf1N1B4_2445at 2774.209 kb on - strand, within Pf1N1B4_2445at 2774.286 kb on + strand, within Pf1N1B4_2445at 2774.470 kb on - strandat 2774.559 kb on + strandat 2774.560 kb on - strand, within Pf1N1B4_2445at 2774.560 kb on - strand, within Pf1N1B4_2445at 2774.619 kb on + strand, within Pf1N1B4_2445at 2774.619 kb on + strand, within Pf1N1B4_2445at 2774.620 kb on - strand, within Pf1N1B4_2445at 2774.620 kb on - strand, within Pf1N1B4_2445at 2774.773 kb on - strandat 2774.817 kb on + strand, within Pf1N1B4_2445at 2774.818 kb on - strand, within Pf1N1B4_2445at 2775.082 kb on + strand, within Pf1N1B4_2445at 2775.151 kb on - strand, within Pf1N1B4_2445at 2775.151 kb on - strand, within Pf1N1B4_2445at 2775.192 kb on + strand, within Pf1N1B4_2445at 2775.192 kb on + strand, within Pf1N1B4_2445at 2775.282 kb on + strandat 2775.282 kb on + strandat 2775.282 kb on + strandat 2775.348 kb on + strandat 2775.349 kb on - strandat 2775.509 kb on + strandat 2775.509 kb on + strandat 2775.509 kb on + strandat 2775.510 kb on - strandat 2775.525 kb on + strandat 2775.531 kb on + strandat 2775.531 kb on + strandat 2775.599 kb on + strandat 2775.599 kb on + strandat 2775.599 kb on + strandat 2775.647 kb on + strandat 2775.647 kb on + strand, within Pf1N1B4_2446at 2775.647 kb on + strand, within Pf1N1B4_2446at 2775.648 kb on - strand, within Pf1N1B4_2446at 2776.049 kb on + strand, within Pf1N1B4_2446at 2776.049 kb on + strand, within Pf1N1B4_2446at 2776.145 kb on + strand, within Pf1N1B4_2446at 2776.145 kb on + strand, within Pf1N1B4_2446at 2776.145 kb on + strand, within Pf1N1B4_2446at 2776.145 kb on + strand, within Pf1N1B4_2446at 2776.145 kb on + strand, within Pf1N1B4_2446at 2776.145 kb on + strand, within Pf1N1B4_2446at 2776.145 kb on + strand, within Pf1N1B4_2446at 2776.146 kb on - strand, within Pf1N1B4_2446at 2776.146 kb on - strand, within Pf1N1B4_2446at 2776.146 kb on - strand, within Pf1N1B4_2446at 2776.146 kb on - strand, within Pf1N1B4_2446at 2776.163 kb on + strand, within Pf1N1B4_2446at 2776.163 kb on + strand, within Pf1N1B4_2446at 2776.163 kb on + strand, within Pf1N1B4_2446at 2776.164 kb on - strand, within Pf1N1B4_2446at 2776.164 kb on - strand, within Pf1N1B4_2446at 2776.211 kb on + strand, within Pf1N1B4_2446at 2776.223 kb on + strand, within Pf1N1B4_2446at 2776.223 kb on + strand, within Pf1N1B4_2446at 2776.223 kb on + strand, within Pf1N1B4_2446at 2776.223 kb on + strand, within Pf1N1B4_2446at 2776.224 kb on - strand, within Pf1N1B4_2446at 2776.224 kb on - strand, within Pf1N1B4_2446at 2776.262 kb on + strand, within Pf1N1B4_2446at 2776.262 kb on + strand, within Pf1N1B4_2446at 2776.263 kb on - strand, within Pf1N1B4_2446at 2776.286 kb on + strand, within Pf1N1B4_2446at 2776.286 kb on + strand, within Pf1N1B4_2446at 2776.286 kb on + strandat 2776.287 kb on - strand, within Pf1N1B4_2446at 2776.452 kb on - strand, within Pf1N1B4_2446

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM
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2,772,617 + Pf1N1B4_2444 0.76 -0.0
2,772,617 + Pf1N1B4_2444 0.76 -1.7
2,772,618 - Pf1N1B4_2444 0.76 -0.3
2,773,029 - Pf1N1B4_2444 0.87 -2.6
2,773,067 + Pf1N1B4_2444 0.89 -2.0
2,773,217 + +0.4
2,773,218 - +0.6
2,773,218 - -0.0
2,773,218 - -1.6
2,773,218 - -2.0
2,773,218 - -1.6
2,773,218 - -0.4
2,773,310 + -1.1
2,773,311 - -0.0
2,773,593 + -0.8
2,773,593 + -0.3
2,773,593 + -2.7
2,773,593 + -2.0
2,773,593 + -2.2
2,773,594 - -1.7
2,773,594 - -2.4
2,773,767 + -0.3
2,773,768 - -1.5
2,773,768 - +0.4
2,773,797 + Pf1N1B4_2445 0.11 -1.5
2,773,797 + Pf1N1B4_2445 0.11 -1.7
2,773,797 + Pf1N1B4_2445 0.11 -1.3
2,773,797 + Pf1N1B4_2445 0.11 -1.7
2,773,797 + Pf1N1B4_2445 0.11 -1.7
2,773,797 + Pf1N1B4_2445 0.11 -1.6
2,773,798 - Pf1N1B4_2445 0.11 -0.2
2,773,798 - Pf1N1B4_2445 0.11 -2.2
2,773,798 - Pf1N1B4_2445 0.11 -0.6
2,773,947 + Pf1N1B4_2445 0.19 -1.2
2,773,947 + Pf1N1B4_2445 0.19 +0.3
2,774,139 + Pf1N1B4_2445 0.30 -2.0
2,774,140 - Pf1N1B4_2445 0.30 -1.3
2,774,140 - Pf1N1B4_2445 0.30 -2.4
2,774,160 + Pf1N1B4_2445 0.31 -0.2
2,774,160 + Pf1N1B4_2445 0.31 -1.2
2,774,161 - Pf1N1B4_2445 0.31 -2.1
2,774,166 + Pf1N1B4_2445 0.31 -1.2
2,774,167 - Pf1N1B4_2445 0.31 -1.0
2,774,206 - Pf1N1B4_2445 0.33 -3.3
2,774,208 + Pf1N1B4_2445 0.33 -0.8
2,774,208 + Pf1N1B4_2445 0.33 -0.7
2,774,208 + Pf1N1B4_2445 0.33 -2.9
2,774,209 - Pf1N1B4_2445 0.33 -0.2
2,774,209 - Pf1N1B4_2445 0.33 -0.6
2,774,286 + Pf1N1B4_2445 0.38 -1.2
2,774,470 - -1.4
2,774,559 + -1.8
2,774,560 - Pf1N1B4_2445 0.52 -2.2
2,774,560 - Pf1N1B4_2445 0.52 -0.4
2,774,619 + Pf1N1B4_2445 0.55 +1.7
2,774,619 + Pf1N1B4_2445 0.55 -1.2
2,774,620 - Pf1N1B4_2445 0.55 +0.6
2,774,620 - Pf1N1B4_2445 0.55 -0.5
2,774,773 - +2.8
2,774,817 + Pf1N1B4_2445 0.66 -1.5
2,774,818 - Pf1N1B4_2445 0.66 -1.7
2,775,082 + Pf1N1B4_2445 0.80 -0.2
2,775,151 - Pf1N1B4_2445 0.84 -1.6
2,775,151 - Pf1N1B4_2445 0.84 -0.2
2,775,192 + Pf1N1B4_2445 0.86 -0.8
2,775,192 + Pf1N1B4_2445 0.86 -1.5
2,775,282 + +0.2
2,775,282 + +0.5
2,775,282 + +0.1
2,775,348 + -0.5
2,775,349 - -1.5
2,775,509 + -3.0
2,775,509 + +0.1
2,775,509 + -2.3
2,775,510 - -1.4
2,775,525 + -1.2
2,775,531 + -1.7
2,775,531 + -1.6
2,775,599 + -4.4
2,775,599 + -0.2
2,775,599 + -0.2
2,775,647 + -1.0
2,775,647 + Pf1N1B4_2446 0.13 +0.2
2,775,647 + Pf1N1B4_2446 0.13 -2.1
2,775,648 - Pf1N1B4_2446 0.14 -1.6
2,776,049 + Pf1N1B4_2446 0.46 -1.2
2,776,049 + Pf1N1B4_2446 0.46 -0.5
2,776,145 + Pf1N1B4_2446 0.53 -2.1
2,776,145 + Pf1N1B4_2446 0.53 -2.2
2,776,145 + Pf1N1B4_2446 0.53 -0.6
2,776,145 + Pf1N1B4_2446 0.53 -2.3
2,776,145 + Pf1N1B4_2446 0.53 -0.8
2,776,145 + Pf1N1B4_2446 0.53 -1.8
2,776,145 + Pf1N1B4_2446 0.53 -0.5
2,776,146 - Pf1N1B4_2446 0.53 -2.0
2,776,146 - Pf1N1B4_2446 0.53 -2.8
2,776,146 - Pf1N1B4_2446 0.53 -0.6
2,776,146 - Pf1N1B4_2446 0.53 +0.7
2,776,163 + Pf1N1B4_2446 0.55 -1.6
2,776,163 + Pf1N1B4_2446 0.55 -1.7
2,776,163 + Pf1N1B4_2446 0.55 +1.4
2,776,164 - Pf1N1B4_2446 0.55 -1.6
2,776,164 - Pf1N1B4_2446 0.55 -3.1
2,776,211 + Pf1N1B4_2446 0.59 -0.8
2,776,223 + Pf1N1B4_2446 0.59 -2.5
2,776,223 + Pf1N1B4_2446 0.59 -2.2
2,776,223 + Pf1N1B4_2446 0.59 +0.9
2,776,223 + Pf1N1B4_2446 0.59 -1.5
2,776,224 - Pf1N1B4_2446 0.60 -2.5
2,776,224 - Pf1N1B4_2446 0.60 +0.4
2,776,262 + Pf1N1B4_2446 0.63 -0.6
2,776,262 + Pf1N1B4_2446 0.63 -1.2
2,776,263 - Pf1N1B4_2446 0.63 -2.7
2,776,286 + Pf1N1B4_2446 0.65 -1.7
2,776,286 + Pf1N1B4_2446 0.65 +0.6
2,776,286 + +0.0
2,776,287 - Pf1N1B4_2446 0.65 -2.5
2,776,452 - Pf1N1B4_2446 0.78 -0.5

Or see this region's nucleotide sequence