Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2444 and Pf1N1B4_2445 are separated by 121 nucleotides Pf1N1B4_2445 and Pf1N1B4_2446 are separated by 26 nucleotides
Pf1N1B4_2444 - Cholesterol oxidase (EC 1.1.3.6), at 2,770,004 to 2,773,462
_2444
Pf1N1B4_2445 - FIG00506946: hypothetical protein, at 2,773,584 to 2,775,452
_2445
Pf1N1B4_2446 - miscellaneous; unknown, at 2,775,479 to 2,776,729
_2446
Position (kb)
2773
2774
2775
2776 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2772.617 kb on + strand, within Pf1N1B4_2444 at 2772.617 kb on + strand, within Pf1N1B4_2444 at 2772.618 kb on - strand, within Pf1N1B4_2444 at 2773.029 kb on - strand, within Pf1N1B4_2444 at 2773.067 kb on + strand, within Pf1N1B4_2444 at 2773.217 kb on + strand at 2773.218 kb on - strand at 2773.218 kb on - strand at 2773.218 kb on - strand at 2773.218 kb on - strand at 2773.218 kb on - strand at 2773.218 kb on - strand at 2773.310 kb on + strand at 2773.311 kb on - strand at 2773.593 kb on + strand at 2773.593 kb on + strand at 2773.593 kb on + strand at 2773.593 kb on + strand at 2773.593 kb on + strand at 2773.594 kb on - strand at 2773.594 kb on - strand at 2773.767 kb on + strand at 2773.768 kb on - strand at 2773.768 kb on - strand at 2773.797 kb on + strand, within Pf1N1B4_2445 at 2773.797 kb on + strand, within Pf1N1B4_2445 at 2773.797 kb on + strand, within Pf1N1B4_2445 at 2773.797 kb on + strand, within Pf1N1B4_2445 at 2773.797 kb on + strand, within Pf1N1B4_2445 at 2773.797 kb on + strand, within Pf1N1B4_2445 at 2773.798 kb on - strand, within Pf1N1B4_2445 at 2773.798 kb on - strand, within Pf1N1B4_2445 at 2773.798 kb on - strand, within Pf1N1B4_2445 at 2773.947 kb on + strand, within Pf1N1B4_2445 at 2773.947 kb on + strand, within Pf1N1B4_2445 at 2774.139 kb on + strand, within Pf1N1B4_2445 at 2774.140 kb on - strand, within Pf1N1B4_2445 at 2774.140 kb on - strand, within Pf1N1B4_2445 at 2774.160 kb on + strand, within Pf1N1B4_2445 at 2774.160 kb on + strand, within Pf1N1B4_2445 at 2774.161 kb on - strand, within Pf1N1B4_2445 at 2774.166 kb on + strand, within Pf1N1B4_2445 at 2774.167 kb on - strand, within Pf1N1B4_2445 at 2774.206 kb on - strand, within Pf1N1B4_2445 at 2774.208 kb on + strand, within Pf1N1B4_2445 at 2774.208 kb on + strand, within Pf1N1B4_2445 at 2774.208 kb on + strand, within Pf1N1B4_2445 at 2774.209 kb on - strand, within Pf1N1B4_2445 at 2774.209 kb on - strand, within Pf1N1B4_2445 at 2774.286 kb on + strand, within Pf1N1B4_2445 at 2774.470 kb on - strand at 2774.559 kb on + strand at 2774.560 kb on - strand, within Pf1N1B4_2445 at 2774.560 kb on - strand, within Pf1N1B4_2445 at 2774.619 kb on + strand, within Pf1N1B4_2445 at 2774.619 kb on + strand, within Pf1N1B4_2445 at 2774.620 kb on - strand, within Pf1N1B4_2445 at 2774.620 kb on - strand, within Pf1N1B4_2445 at 2774.773 kb on - strand at 2774.817 kb on + strand, within Pf1N1B4_2445 at 2774.818 kb on - strand, within Pf1N1B4_2445 at 2775.082 kb on + strand, within Pf1N1B4_2445 at 2775.151 kb on - strand, within Pf1N1B4_2445 at 2775.151 kb on - strand, within Pf1N1B4_2445 at 2775.192 kb on + strand, within Pf1N1B4_2445 at 2775.192 kb on + strand, within Pf1N1B4_2445 at 2775.282 kb on + strand at 2775.282 kb on + strand at 2775.282 kb on + strand at 2775.348 kb on + strand at 2775.349 kb on - strand at 2775.509 kb on + strand at 2775.509 kb on + strand at 2775.509 kb on + strand at 2775.510 kb on - strand at 2775.525 kb on + strand at 2775.531 kb on + strand at 2775.531 kb on + strand at 2775.599 kb on + strand at 2775.599 kb on + strand at 2775.599 kb on + strand at 2775.647 kb on + strand at 2775.647 kb on + strand, within Pf1N1B4_2446 at 2775.647 kb on + strand, within Pf1N1B4_2446 at 2775.648 kb on - strand, within Pf1N1B4_2446 at 2776.049 kb on + strand, within Pf1N1B4_2446 at 2776.049 kb on + strand, within Pf1N1B4_2446 at 2776.145 kb on + strand, within Pf1N1B4_2446 at 2776.145 kb on + strand, within Pf1N1B4_2446 at 2776.145 kb on + strand, within Pf1N1B4_2446 at 2776.145 kb on + strand, within Pf1N1B4_2446 at 2776.145 kb on + strand, within Pf1N1B4_2446 at 2776.145 kb on + strand, within Pf1N1B4_2446 at 2776.145 kb on + strand, within Pf1N1B4_2446 at 2776.146 kb on - strand, within Pf1N1B4_2446 at 2776.146 kb on - strand, within Pf1N1B4_2446 at 2776.146 kb on - strand, within Pf1N1B4_2446 at 2776.146 kb on - strand, within Pf1N1B4_2446 at 2776.163 kb on + strand, within Pf1N1B4_2446 at 2776.163 kb on + strand, within Pf1N1B4_2446 at 2776.163 kb on + strand, within Pf1N1B4_2446 at 2776.164 kb on - strand, within Pf1N1B4_2446 at 2776.164 kb on - strand, within Pf1N1B4_2446 at 2776.211 kb on + strand, within Pf1N1B4_2446 at 2776.223 kb on + strand, within Pf1N1B4_2446 at 2776.223 kb on + strand, within Pf1N1B4_2446 at 2776.223 kb on + strand, within Pf1N1B4_2446 at 2776.223 kb on + strand, within Pf1N1B4_2446 at 2776.224 kb on - strand, within Pf1N1B4_2446 at 2776.224 kb on - strand, within Pf1N1B4_2446 at 2776.262 kb on + strand, within Pf1N1B4_2446 at 2776.262 kb on + strand, within Pf1N1B4_2446 at 2776.263 kb on - strand, within Pf1N1B4_2446 at 2776.286 kb on + strand, within Pf1N1B4_2446 at 2776.286 kb on + strand, within Pf1N1B4_2446 at 2776.286 kb on + strand at 2776.287 kb on - strand, within Pf1N1B4_2446 at 2776.452 kb on - strand, within Pf1N1B4_2446
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 2,772,617 + Pf1N1B4_2444 0.76 -0.0 2,772,617 + Pf1N1B4_2444 0.76 -1.7 2,772,618 - Pf1N1B4_2444 0.76 -0.3 2,773,029 - Pf1N1B4_2444 0.87 -2.6 2,773,067 + Pf1N1B4_2444 0.89 -2.0 2,773,217 + +0.4 2,773,218 - +0.6 2,773,218 - -0.0 2,773,218 - -1.6 2,773,218 - -2.0 2,773,218 - -1.6 2,773,218 - -0.4 2,773,310 + -1.1 2,773,311 - -0.0 2,773,593 + -0.8 2,773,593 + -0.3 2,773,593 + -2.7 2,773,593 + -2.0 2,773,593 + -2.2 2,773,594 - -1.7 2,773,594 - -2.4 2,773,767 + -0.3 2,773,768 - -1.5 2,773,768 - +0.4 2,773,797 + Pf1N1B4_2445 0.11 -1.5 2,773,797 + Pf1N1B4_2445 0.11 -1.7 2,773,797 + Pf1N1B4_2445 0.11 -1.3 2,773,797 + Pf1N1B4_2445 0.11 -1.7 2,773,797 + Pf1N1B4_2445 0.11 -1.7 2,773,797 + Pf1N1B4_2445 0.11 -1.6 2,773,798 - Pf1N1B4_2445 0.11 -0.2 2,773,798 - Pf1N1B4_2445 0.11 -2.2 2,773,798 - Pf1N1B4_2445 0.11 -0.6 2,773,947 + Pf1N1B4_2445 0.19 -1.2 2,773,947 + Pf1N1B4_2445 0.19 +0.3 2,774,139 + Pf1N1B4_2445 0.30 -2.0 2,774,140 - Pf1N1B4_2445 0.30 -1.3 2,774,140 - Pf1N1B4_2445 0.30 -2.4 2,774,160 + Pf1N1B4_2445 0.31 -0.2 2,774,160 + Pf1N1B4_2445 0.31 -1.2 2,774,161 - Pf1N1B4_2445 0.31 -2.1 2,774,166 + Pf1N1B4_2445 0.31 -1.2 2,774,167 - Pf1N1B4_2445 0.31 -1.0 2,774,206 - Pf1N1B4_2445 0.33 -3.3 2,774,208 + Pf1N1B4_2445 0.33 -0.8 2,774,208 + Pf1N1B4_2445 0.33 -0.7 2,774,208 + Pf1N1B4_2445 0.33 -2.9 2,774,209 - Pf1N1B4_2445 0.33 -0.2 2,774,209 - Pf1N1B4_2445 0.33 -0.6 2,774,286 + Pf1N1B4_2445 0.38 -1.2 2,774,470 - -1.4 2,774,559 + -1.8 2,774,560 - Pf1N1B4_2445 0.52 -2.2 2,774,560 - Pf1N1B4_2445 0.52 -0.4 2,774,619 + Pf1N1B4_2445 0.55 +1.7 2,774,619 + Pf1N1B4_2445 0.55 -1.2 2,774,620 - Pf1N1B4_2445 0.55 +0.6 2,774,620 - Pf1N1B4_2445 0.55 -0.5 2,774,773 - +2.8 2,774,817 + Pf1N1B4_2445 0.66 -1.5 2,774,818 - Pf1N1B4_2445 0.66 -1.7 2,775,082 + Pf1N1B4_2445 0.80 -0.2 2,775,151 - Pf1N1B4_2445 0.84 -1.6 2,775,151 - Pf1N1B4_2445 0.84 -0.2 2,775,192 + Pf1N1B4_2445 0.86 -0.8 2,775,192 + Pf1N1B4_2445 0.86 -1.5 2,775,282 + +0.2 2,775,282 + +0.5 2,775,282 + +0.1 2,775,348 + -0.5 2,775,349 - -1.5 2,775,509 + -3.0 2,775,509 + +0.1 2,775,509 + -2.3 2,775,510 - -1.4 2,775,525 + -1.2 2,775,531 + -1.7 2,775,531 + -1.6 2,775,599 + -4.4 2,775,599 + -0.2 2,775,599 + -0.2 2,775,647 + -1.0 2,775,647 + Pf1N1B4_2446 0.13 +0.2 2,775,647 + Pf1N1B4_2446 0.13 -2.1 2,775,648 - Pf1N1B4_2446 0.14 -1.6 2,776,049 + Pf1N1B4_2446 0.46 -1.2 2,776,049 + Pf1N1B4_2446 0.46 -0.5 2,776,145 + Pf1N1B4_2446 0.53 -2.1 2,776,145 + Pf1N1B4_2446 0.53 -2.2 2,776,145 + Pf1N1B4_2446 0.53 -0.6 2,776,145 + Pf1N1B4_2446 0.53 -2.3 2,776,145 + Pf1N1B4_2446 0.53 -0.8 2,776,145 + Pf1N1B4_2446 0.53 -1.8 2,776,145 + Pf1N1B4_2446 0.53 -0.5 2,776,146 - Pf1N1B4_2446 0.53 -2.0 2,776,146 - Pf1N1B4_2446 0.53 -2.8 2,776,146 - Pf1N1B4_2446 0.53 -0.6 2,776,146 - Pf1N1B4_2446 0.53 +0.7 2,776,163 + Pf1N1B4_2446 0.55 -1.6 2,776,163 + Pf1N1B4_2446 0.55 -1.7 2,776,163 + Pf1N1B4_2446 0.55 +1.4 2,776,164 - Pf1N1B4_2446 0.55 -1.6 2,776,164 - Pf1N1B4_2446 0.55 -3.1 2,776,211 + Pf1N1B4_2446 0.59 -0.8 2,776,223 + Pf1N1B4_2446 0.59 -2.5 2,776,223 + Pf1N1B4_2446 0.59 -2.2 2,776,223 + Pf1N1B4_2446 0.59 +0.9 2,776,223 + Pf1N1B4_2446 0.59 -1.5 2,776,224 - Pf1N1B4_2446 0.60 -2.5 2,776,224 - Pf1N1B4_2446 0.60 +0.4 2,776,262 + Pf1N1B4_2446 0.63 -0.6 2,776,262 + Pf1N1B4_2446 0.63 -1.2 2,776,263 - Pf1N1B4_2446 0.63 -2.7 2,776,286 + Pf1N1B4_2446 0.65 -1.7 2,776,286 + Pf1N1B4_2446 0.65 +0.6 2,776,286 + +0.0 2,776,287 - Pf1N1B4_2446 0.65 -2.5 2,776,452 - Pf1N1B4_2446 0.78 -0.5
Or see this region's nucleotide sequence