Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2444 and Pf1N1B4_2445 are separated by 121 nucleotides
Pf1N1B4_2444 - Cholesterol oxidase (EC 1.1.3.6), at 2,770,004 to 2,773,462
_2444
Pf1N1B4_2445 - FIG00506946: hypothetical protein, at 2,773,584 to 2,775,452
_2445
Position (kb)
2770
2771
2772
2773
2774 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2769.163 kb on - strand at 2769.218 kb on - strand at 2769.243 kb on + strand at 2769.243 kb on + strand at 2769.244 kb on - strand at 2769.246 kb on - strand at 2769.247 kb on + strand at 2769.248 kb on - strand at 2769.248 kb on - strand at 2769.248 kb on - strand at 2769.278 kb on - strand at 2769.427 kb on + strand at 2769.428 kb on - strand at 2769.429 kb on + strand at 2769.429 kb on + strand at 2769.429 kb on + strand at 2769.429 kb on + strand at 2769.429 kb on + strand at 2769.430 kb on - strand at 2769.430 kb on - strand at 2769.430 kb on - strand at 2769.477 kb on + strand at 2769.477 kb on + strand at 2769.496 kb on - strand at 2769.526 kb on + strand at 2769.526 kb on + strand at 2769.526 kb on + strand at 2769.526 kb on + strand at 2769.528 kb on + strand at 2769.528 kb on + strand at 2769.528 kb on + strand at 2769.528 kb on + strand at 2769.559 kb on + strand at 2769.559 kb on + strand at 2769.655 kb on + strand at 2769.656 kb on - strand at 2769.705 kb on + strand at 2769.706 kb on - strand at 2769.706 kb on - strand at 2769.868 kb on - strand at 2769.869 kb on + strand at 2769.886 kb on - strand at 2769.886 kb on - strand at 2769.886 kb on - strand at 2769.951 kb on + strand at 2769.952 kb on - strand at 2770.046 kb on + strand at 2770.047 kb on - strand at 2770.171 kb on + strand at 2770.529 kb on + strand, within Pf1N1B4_2444 at 2770.530 kb on - strand, within Pf1N1B4_2444 at 2770.636 kb on + strand, within Pf1N1B4_2444 at 2770.670 kb on + strand, within Pf1N1B4_2444 at 2770.671 kb on - strand, within Pf1N1B4_2444 at 2770.775 kb on + strand, within Pf1N1B4_2444 at 2771.207 kb on + strand, within Pf1N1B4_2444 at 2771.276 kb on + strand at 2771.277 kb on - strand, within Pf1N1B4_2444 at 2771.277 kb on - strand, within Pf1N1B4_2444 at 2771.277 kb on - strand, within Pf1N1B4_2444 at 2771.550 kb on + strand at 2771.551 kb on - strand, within Pf1N1B4_2444 at 2771.580 kb on - strand, within Pf1N1B4_2444 at 2771.580 kb on - strand, within Pf1N1B4_2444 at 2771.580 kb on - strand, within Pf1N1B4_2444 at 2771.580 kb on - strand, within Pf1N1B4_2444 at 2771.592 kb on - strand, within Pf1N1B4_2444 at 2771.592 kb on - strand, within Pf1N1B4_2444 at 2771.592 kb on - strand, within Pf1N1B4_2444 at 2771.592 kb on - strand, within Pf1N1B4_2444 at 2771.789 kb on + strand, within Pf1N1B4_2444 at 2771.790 kb on - strand, within Pf1N1B4_2444 at 2771.790 kb on - strand, within Pf1N1B4_2444 at 2771.837 kb on + strand, within Pf1N1B4_2444 at 2772.032 kb on + strand, within Pf1N1B4_2444 at 2772.032 kb on + strand, within Pf1N1B4_2444 at 2772.066 kb on - strand, within Pf1N1B4_2444 at 2772.066 kb on - strand, within Pf1N1B4_2444 at 2772.066 kb on - strand, within Pf1N1B4_2444 at 2772.066 kb on - strand, within Pf1N1B4_2444 at 2772.101 kb on + strand, within Pf1N1B4_2444 at 2772.101 kb on + strand, within Pf1N1B4_2444 at 2772.102 kb on - strand, within Pf1N1B4_2444 at 2772.104 kb on + strand, within Pf1N1B4_2444 at 2772.104 kb on + strand, within Pf1N1B4_2444 at 2772.105 kb on - strand, within Pf1N1B4_2444 at 2772.176 kb on + strand, within Pf1N1B4_2444 at 2772.176 kb on + strand, within Pf1N1B4_2444 at 2772.177 kb on - strand, within Pf1N1B4_2444 at 2772.392 kb on + strand, within Pf1N1B4_2444 at 2772.392 kb on + strand, within Pf1N1B4_2444 at 2772.392 kb on + strand, within Pf1N1B4_2444 at 2772.392 kb on + strand, within Pf1N1B4_2444 at 2772.392 kb on + strand, within Pf1N1B4_2444 at 2772.393 kb on - strand, within Pf1N1B4_2444 at 2772.393 kb on - strand, within Pf1N1B4_2444 at 2772.393 kb on - strand, within Pf1N1B4_2444 at 2772.393 kb on - strand, within Pf1N1B4_2444 at 2772.393 kb on - strand, within Pf1N1B4_2444 at 2772.393 kb on - strand, within Pf1N1B4_2444 at 2772.480 kb on + strand, within Pf1N1B4_2444 at 2772.480 kb on + strand, within Pf1N1B4_2444 at 2772.480 kb on + strand, within Pf1N1B4_2444 at 2772.480 kb on + strand, within Pf1N1B4_2444 at 2772.480 kb on + strand, within Pf1N1B4_2444 at 2772.480 kb on + strand, within Pf1N1B4_2444 at 2772.480 kb on + strand, within Pf1N1B4_2444 at 2772.480 kb on + strand, within Pf1N1B4_2444 at 2772.481 kb on - strand, within Pf1N1B4_2444 at 2772.481 kb on - strand, within Pf1N1B4_2444 at 2772.481 kb on - strand at 2772.481 kb on - strand, within Pf1N1B4_2444 at 2772.481 kb on - strand, within Pf1N1B4_2444 at 2772.481 kb on - strand, within Pf1N1B4_2444 at 2772.560 kb on - strand, within Pf1N1B4_2444 at 2772.560 kb on - strand, within Pf1N1B4_2444 at 2772.617 kb on + strand, within Pf1N1B4_2444 at 2772.617 kb on + strand, within Pf1N1B4_2444 at 2772.618 kb on - strand, within Pf1N1B4_2444 at 2773.029 kb on - strand, within Pf1N1B4_2444 at 2773.067 kb on + strand, within Pf1N1B4_2444 at 2773.217 kb on + strand at 2773.218 kb on - strand at 2773.218 kb on - strand at 2773.218 kb on - strand at 2773.218 kb on - strand at 2773.218 kb on - strand at 2773.218 kb on - strand at 2773.310 kb on + strand at 2773.311 kb on - strand at 2773.593 kb on + strand at 2773.593 kb on + strand at 2773.593 kb on + strand at 2773.593 kb on + strand at 2773.593 kb on + strand at 2773.594 kb on - strand at 2773.594 kb on - strand at 2773.767 kb on + strand at 2773.768 kb on - strand at 2773.768 kb on - strand at 2773.797 kb on + strand, within Pf1N1B4_2445 at 2773.797 kb on + strand, within Pf1N1B4_2445 at 2773.797 kb on + strand, within Pf1N1B4_2445 at 2773.797 kb on + strand, within Pf1N1B4_2445 at 2773.797 kb on + strand, within Pf1N1B4_2445 at 2773.797 kb on + strand, within Pf1N1B4_2445 at 2773.798 kb on - strand, within Pf1N1B4_2445 at 2773.798 kb on - strand, within Pf1N1B4_2445 at 2773.798 kb on - strand, within Pf1N1B4_2445 at 2773.947 kb on + strand, within Pf1N1B4_2445 at 2773.947 kb on + strand, within Pf1N1B4_2445 at 2774.139 kb on + strand, within Pf1N1B4_2445 at 2774.140 kb on - strand, within Pf1N1B4_2445 at 2774.140 kb on - strand, within Pf1N1B4_2445 at 2774.160 kb on + strand, within Pf1N1B4_2445 at 2774.160 kb on + strand, within Pf1N1B4_2445 at 2774.161 kb on - strand, within Pf1N1B4_2445 at 2774.166 kb on + strand, within Pf1N1B4_2445 at 2774.167 kb on - strand, within Pf1N1B4_2445 at 2774.206 kb on - strand, within Pf1N1B4_2445 at 2774.208 kb on + strand, within Pf1N1B4_2445 at 2774.208 kb on + strand, within Pf1N1B4_2445 at 2774.208 kb on + strand, within Pf1N1B4_2445 at 2774.209 kb on - strand, within Pf1N1B4_2445 at 2774.209 kb on - strand, within Pf1N1B4_2445 at 2774.286 kb on + strand, within Pf1N1B4_2445
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 2,769,163 - +0.1 2,769,218 - +1.5 2,769,243 + -0.6 2,769,243 + +1.0 2,769,244 - -1.2 2,769,246 - +0.3 2,769,247 + -0.7 2,769,248 - -2.2 2,769,248 - -2.5 2,769,248 - +0.7 2,769,278 - -0.8 2,769,427 + -2.5 2,769,428 - +1.3 2,769,429 + +1.6 2,769,429 + +0.0 2,769,429 + -0.4 2,769,429 + -0.2 2,769,429 + -2.2 2,769,430 - +0.9 2,769,430 - -1.2 2,769,430 - +1.0 2,769,477 + -1.6 2,769,477 + +0.6 2,769,496 - +0.4 2,769,526 + -0.4 2,769,526 + +1.4 2,769,526 + +1.6 2,769,526 + -0.2 2,769,528 + +0.4 2,769,528 + +0.3 2,769,528 + -0.2 2,769,528 + -1.1 2,769,559 + +0.2 2,769,559 + +0.4 2,769,655 + +0.6 2,769,656 - -2.1 2,769,705 + +0.8 2,769,706 - -2.4 2,769,706 - -1.6 2,769,868 - +0.5 2,769,869 + +1.0 2,769,886 - -2.4 2,769,886 - -0.2 2,769,886 - +1.5 2,769,951 + +0.6 2,769,952 - +0.4 2,770,046 + +1.8 2,770,047 - -2.3 2,770,171 + -1.9 2,770,529 + Pf1N1B4_2444 0.15 -2.0 2,770,530 - Pf1N1B4_2444 0.15 -1.3 2,770,636 + Pf1N1B4_2444 0.18 -0.9 2,770,670 + Pf1N1B4_2444 0.19 -2.2 2,770,671 - Pf1N1B4_2444 0.19 -1.4 2,770,775 + Pf1N1B4_2444 0.22 -1.2 2,771,207 + Pf1N1B4_2444 0.35 -0.7 2,771,276 + -0.6 2,771,277 - Pf1N1B4_2444 0.37 -2.5 2,771,277 - Pf1N1B4_2444 0.37 -1.6 2,771,277 - Pf1N1B4_2444 0.37 -1.6 2,771,550 + -0.0 2,771,551 - Pf1N1B4_2444 0.45 -0.8 2,771,580 - Pf1N1B4_2444 0.46 -2.7 2,771,580 - Pf1N1B4_2444 0.46 -1.0 2,771,580 - Pf1N1B4_2444 0.46 -0.4 2,771,580 - Pf1N1B4_2444 0.46 -0.6 2,771,592 - Pf1N1B4_2444 0.46 -1.7 2,771,592 - Pf1N1B4_2444 0.46 -3.3 2,771,592 - Pf1N1B4_2444 0.46 -1.8 2,771,592 - Pf1N1B4_2444 0.46 -3.2 2,771,789 + Pf1N1B4_2444 0.52 +1.9 2,771,790 - Pf1N1B4_2444 0.52 -2.0 2,771,790 - Pf1N1B4_2444 0.52 -2.2 2,771,837 + Pf1N1B4_2444 0.53 +0.0 2,772,032 + Pf1N1B4_2444 0.59 -2.0 2,772,032 + Pf1N1B4_2444 0.59 -0.8 2,772,066 - Pf1N1B4_2444 0.60 -2.0 2,772,066 - Pf1N1B4_2444 0.60 -2.3 2,772,066 - Pf1N1B4_2444 0.60 -1.5 2,772,066 - Pf1N1B4_2444 0.60 -2.0 2,772,101 + Pf1N1B4_2444 0.61 -2.4 2,772,101 + Pf1N1B4_2444 0.61 -2.8 2,772,102 - Pf1N1B4_2444 0.61 -2.6 2,772,104 + Pf1N1B4_2444 0.61 +0.4 2,772,104 + Pf1N1B4_2444 0.61 -0.2 2,772,105 - Pf1N1B4_2444 0.61 +0.4 2,772,176 + Pf1N1B4_2444 0.63 -1.0 2,772,176 + Pf1N1B4_2444 0.63 -3.0 2,772,177 - Pf1N1B4_2444 0.63 -1.7 2,772,392 + Pf1N1B4_2444 0.69 -1.2 2,772,392 + Pf1N1B4_2444 0.69 -1.0 2,772,392 + Pf1N1B4_2444 0.69 -1.1 2,772,392 + Pf1N1B4_2444 0.69 -1.3 2,772,392 + Pf1N1B4_2444 0.69 -0.6 2,772,393 - Pf1N1B4_2444 0.69 -2.9 2,772,393 - Pf1N1B4_2444 0.69 -1.7 2,772,393 - Pf1N1B4_2444 0.69 -0.5 2,772,393 - Pf1N1B4_2444 0.69 +0.2 2,772,393 - Pf1N1B4_2444 0.69 -0.4 2,772,393 - Pf1N1B4_2444 0.69 -0.9 2,772,480 + Pf1N1B4_2444 0.72 +0.4 2,772,480 + Pf1N1B4_2444 0.72 -0.1 2,772,480 + Pf1N1B4_2444 0.72 -2.3 2,772,480 + Pf1N1B4_2444 0.72 +0.6 2,772,480 + Pf1N1B4_2444 0.72 -0.3 2,772,480 + Pf1N1B4_2444 0.72 -0.2 2,772,480 + Pf1N1B4_2444 0.72 -0.3 2,772,480 + Pf1N1B4_2444 0.72 -1.4 2,772,481 - Pf1N1B4_2444 0.72 +1.2 2,772,481 - Pf1N1B4_2444 0.72 -1.7 2,772,481 - -0.8 2,772,481 - Pf1N1B4_2444 0.72 -0.6 2,772,481 - Pf1N1B4_2444 0.72 -4.0 2,772,481 - Pf1N1B4_2444 0.72 -0.9 2,772,560 - Pf1N1B4_2444 0.74 -0.4 2,772,560 - Pf1N1B4_2444 0.74 -2.6 2,772,617 + Pf1N1B4_2444 0.76 -0.0 2,772,617 + Pf1N1B4_2444 0.76 -1.7 2,772,618 - Pf1N1B4_2444 0.76 -0.3 2,773,029 - Pf1N1B4_2444 0.87 -2.6 2,773,067 + Pf1N1B4_2444 0.89 -2.0 2,773,217 + +0.4 2,773,218 - +0.6 2,773,218 - -0.0 2,773,218 - -1.6 2,773,218 - -2.0 2,773,218 - -1.6 2,773,218 - -0.4 2,773,310 + -1.1 2,773,311 - -0.0 2,773,593 + -0.8 2,773,593 + -0.3 2,773,593 + -2.7 2,773,593 + -2.0 2,773,593 + -2.2 2,773,594 - -1.7 2,773,594 - -2.4 2,773,767 + -0.3 2,773,768 - -1.5 2,773,768 - +0.4 2,773,797 + Pf1N1B4_2445 0.11 -1.5 2,773,797 + Pf1N1B4_2445 0.11 -1.7 2,773,797 + Pf1N1B4_2445 0.11 -1.3 2,773,797 + Pf1N1B4_2445 0.11 -1.7 2,773,797 + Pf1N1B4_2445 0.11 -1.7 2,773,797 + Pf1N1B4_2445 0.11 -1.6 2,773,798 - Pf1N1B4_2445 0.11 -0.2 2,773,798 - Pf1N1B4_2445 0.11 -2.2 2,773,798 - Pf1N1B4_2445 0.11 -0.6 2,773,947 + Pf1N1B4_2445 0.19 -1.2 2,773,947 + Pf1N1B4_2445 0.19 +0.3 2,774,139 + Pf1N1B4_2445 0.30 -2.0 2,774,140 - Pf1N1B4_2445 0.30 -1.3 2,774,140 - Pf1N1B4_2445 0.30 -2.4 2,774,160 + Pf1N1B4_2445 0.31 -0.2 2,774,160 + Pf1N1B4_2445 0.31 -1.2 2,774,161 - Pf1N1B4_2445 0.31 -2.1 2,774,166 + Pf1N1B4_2445 0.31 -1.2 2,774,167 - Pf1N1B4_2445 0.31 -1.0 2,774,206 - Pf1N1B4_2445 0.33 -3.3 2,774,208 + Pf1N1B4_2445 0.33 -0.8 2,774,208 + Pf1N1B4_2445 0.33 -0.7 2,774,208 + Pf1N1B4_2445 0.33 -2.9 2,774,209 - Pf1N1B4_2445 0.33 -0.2 2,774,209 - Pf1N1B4_2445 0.33 -0.6 2,774,286 + Pf1N1B4_2445 0.38 -1.2
Or see this region's nucleotide sequence