Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2394 and Pf1N1B4_2395 are separated by 293 nucleotides Pf1N1B4_2395 and Pf1N1B4_2396 are separated by 18 nucleotides
Pf1N1B4_2394 - DNA ligase (EC 6.5.1.2), at 2,710,998 to 2,712,677
_2394
Pf1N1B4_2395 - S-adenosylmethionine synthetase (EC 2.5.1.6), at 2,712,971 to 2,714,161
_2395
Pf1N1B4_2396 - Transcriptional regulator, ArsR family / Methyltransferase fusion, at 2,714,180 to 2,715,175
_2396
Position (kb)
2712
2713
2714
2715 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2712.105 kb on + strand, within Pf1N1B4_2394 at 2712.105 kb on + strand, within Pf1N1B4_2394 at 2712.105 kb on + strand, within Pf1N1B4_2394 at 2712.106 kb on - strand, within Pf1N1B4_2394 at 2712.127 kb on + strand, within Pf1N1B4_2394 at 2712.128 kb on - strand, within Pf1N1B4_2394 at 2712.128 kb on - strand, within Pf1N1B4_2394 at 2712.268 kb on + strand, within Pf1N1B4_2394 at 2712.487 kb on + strand, within Pf1N1B4_2394 at 2712.487 kb on + strand, within Pf1N1B4_2394 at 2712.487 kb on + strand, within Pf1N1B4_2394 at 2712.488 kb on - strand, within Pf1N1B4_2394 at 2712.526 kb on + strand at 2712.526 kb on + strand at 2712.526 kb on + strand at 2712.526 kb on + strand at 2712.527 kb on - strand at 2712.713 kb on + strand at 2712.713 kb on + strand at 2712.713 kb on + strand at 2712.714 kb on - strand at 2712.714 kb on - strand at 2712.714 kb on - strand at 2712.714 kb on - strand at 2712.832 kb on - strand at 2712.972 kb on + strand at 2712.973 kb on - strand at 2712.973 kb on - strand at 2714.243 kb on - strand at 2714.247 kb on - strand at 2714.247 kb on - strand at 2714.247 kb on - strand at 2714.247 kb on - strand at 2714.247 kb on - strand at 2714.253 kb on - strand at 2714.253 kb on - strand at 2714.253 kb on - strand at 2714.253 kb on - strand at 2714.253 kb on - strand at 2714.253 kb on - strand at 2714.253 kb on - strand at 2714.253 kb on - strand at 2714.253 kb on - strand at 2714.253 kb on - strand at 2714.257 kb on - strand at 2714.257 kb on - strand at 2714.257 kb on - strand at 2714.257 kb on - strand at 2714.257 kb on - strand at 2714.257 kb on - strand at 2714.257 kb on - strand at 2714.376 kb on - strand, within Pf1N1B4_2396 at 2714.376 kb on - strand, within Pf1N1B4_2396 at 2714.376 kb on - strand, within Pf1N1B4_2396 at 2714.376 kb on - strand, within Pf1N1B4_2396 at 2714.376 kb on - strand, within Pf1N1B4_2396 at 2714.376 kb on - strand, within Pf1N1B4_2396 at 2714.376 kb on - strand, within Pf1N1B4_2396 at 2714.376 kb on - strand, within Pf1N1B4_2396 at 2714.376 kb on - strand, within Pf1N1B4_2396 at 2714.376 kb on - strand, within Pf1N1B4_2396 at 2714.463 kb on - strand, within Pf1N1B4_2396 at 2714.463 kb on - strand, within Pf1N1B4_2396 at 2714.463 kb on - strand, within Pf1N1B4_2396 at 2714.463 kb on - strand, within Pf1N1B4_2396 at 2714.475 kb on - strand, within Pf1N1B4_2396 at 2714.475 kb on - strand, within Pf1N1B4_2396 at 2714.475 kb on - strand, within Pf1N1B4_2396 at 2714.475 kb on - strand, within Pf1N1B4_2396 at 2714.499 kb on - strand, within Pf1N1B4_2396 at 2714.715 kb on - strand, within Pf1N1B4_2396 at 2715.051 kb on - strand, within Pf1N1B4_2396 at 2715.084 kb on - strand at 2715.084 kb on - strand at 2715.084 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 2,712,105 + Pf1N1B4_2394 0.66 -2.0 2,712,105 + Pf1N1B4_2394 0.66 +1.2 2,712,105 + Pf1N1B4_2394 0.66 +0.2 2,712,106 - Pf1N1B4_2394 0.66 -0.2 2,712,127 + Pf1N1B4_2394 0.67 -0.7 2,712,128 - Pf1N1B4_2394 0.67 -0.0 2,712,128 - Pf1N1B4_2394 0.67 +0.3 2,712,268 + Pf1N1B4_2394 0.76 -0.3 2,712,487 + Pf1N1B4_2394 0.89 +1.0 2,712,487 + Pf1N1B4_2394 0.89 -0.3 2,712,487 + Pf1N1B4_2394 0.89 +0.0 2,712,488 - Pf1N1B4_2394 0.89 +0.9 2,712,526 + -0.4 2,712,526 + -2.1 2,712,526 + -0.5 2,712,526 + +1.3 2,712,527 - -0.6 2,712,713 + +0.2 2,712,713 + -2.0 2,712,713 + +1.0 2,712,714 - +0.1 2,712,714 - -0.6 2,712,714 - -2.3 2,712,714 - +1.4 2,712,832 - -0.7 2,712,972 + -0.6 2,712,973 - +0.4 2,712,973 - +1.3 2,714,243 - +0.2 2,714,247 - +0.7 2,714,247 - +1.0 2,714,247 - +0.4 2,714,247 - +0.4 2,714,247 - +0.4 2,714,253 - +0.3 2,714,253 - +1.1 2,714,253 - -0.4 2,714,253 - -0.6 2,714,253 - +0.2 2,714,253 - -0.1 2,714,253 - -0.9 2,714,253 - -0.3 2,714,253 - +1.4 2,714,253 - -1.0 2,714,257 - +1.5 2,714,257 - +0.7 2,714,257 - -2.1 2,714,257 - -0.2 2,714,257 - -1.0 2,714,257 - +1.0 2,714,257 - -1.5 2,714,376 - Pf1N1B4_2396 0.20 +0.4 2,714,376 - Pf1N1B4_2396 0.20 +1.8 2,714,376 - Pf1N1B4_2396 0.20 +0.7 2,714,376 - Pf1N1B4_2396 0.20 -0.3 2,714,376 - Pf1N1B4_2396 0.20 +0.8 2,714,376 - Pf1N1B4_2396 0.20 +1.1 2,714,376 - Pf1N1B4_2396 0.20 +0.4 2,714,376 - Pf1N1B4_2396 0.20 -0.4 2,714,376 - Pf1N1B4_2396 0.20 +0.4 2,714,376 - Pf1N1B4_2396 0.20 -1.4 2,714,463 - Pf1N1B4_2396 0.28 +0.8 2,714,463 - Pf1N1B4_2396 0.28 +1.2 2,714,463 - Pf1N1B4_2396 0.28 -1.7 2,714,463 - Pf1N1B4_2396 0.28 -0.6 2,714,475 - Pf1N1B4_2396 0.30 +0.9 2,714,475 - Pf1N1B4_2396 0.30 -0.2 2,714,475 - Pf1N1B4_2396 0.30 -0.9 2,714,475 - Pf1N1B4_2396 0.30 -1.4 2,714,499 - Pf1N1B4_2396 0.32 -1.1 2,714,715 - Pf1N1B4_2396 0.54 -0.6 2,715,051 - Pf1N1B4_2396 0.87 -0.6 2,715,084 - -0.2 2,715,084 - -0.6 2,715,084 - -2.0
Or see this region's nucleotide sequence