Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2384 and Pf1N1B4_2385 are separated by 160 nucleotides Pf1N1B4_2385 and Pf1N1B4_2386 are separated by 325 nucleotides Pf1N1B4_2386 and Pf1N1B4_2387 are separated by 205 nucleotides
Pf1N1B4_2384 - 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20), at 2,701,560 to 2,702,405
_2384
Pf1N1B4_2385 - Adenosylhomocysteinase (EC 3.3.1.1), at 2,702,566 to 2,703,975
_2385
Pf1N1B4_2386 - cytosolic long-chain acyl-CoA thioester hydrolase family protein, at 2,704,301 to 2,704,705
_2386
Pf1N1B4_2387 - Putative transport, at 2,704,911 to 2,705,798
_2387
Position (kb)
2702
2703
2704 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2701.621 kb on + strand at 2701.622 kb on - strand at 2701.622 kb on - strand at 2701.721 kb on - strand, within Pf1N1B4_2384 at 2701.795 kb on + strand, within Pf1N1B4_2384 at 2701.795 kb on + strand, within Pf1N1B4_2384 at 2701.795 kb on + strand, within Pf1N1B4_2384 at 2701.795 kb on + strand, within Pf1N1B4_2384 at 2701.795 kb on + strand, within Pf1N1B4_2384 at 2701.795 kb on + strand, within Pf1N1B4_2384 at 2701.795 kb on + strand, within Pf1N1B4_2384 at 2701.795 kb on + strand, within Pf1N1B4_2384 at 2701.796 kb on - strand, within Pf1N1B4_2384 at 2701.796 kb on - strand, within Pf1N1B4_2384 at 2701.796 kb on - strand, within Pf1N1B4_2384 at 2701.796 kb on - strand, within Pf1N1B4_2384 at 2701.796 kb on - strand, within Pf1N1B4_2384 at 2701.796 kb on - strand, within Pf1N1B4_2384 at 2701.796 kb on - strand, within Pf1N1B4_2384 at 2701.867 kb on + strand, within Pf1N1B4_2384 at 2701.867 kb on + strand, within Pf1N1B4_2384 at 2701.868 kb on - strand, within Pf1N1B4_2384 at 2701.868 kb on - strand, within Pf1N1B4_2384 at 2701.868 kb on - strand, within Pf1N1B4_2384 at 2701.868 kb on - strand, within Pf1N1B4_2384 at 2701.868 kb on - strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.873 kb on + strand, within Pf1N1B4_2384 at 2701.874 kb on - strand, within Pf1N1B4_2384 at 2701.874 kb on - strand, within Pf1N1B4_2384 at 2701.874 kb on - strand, within Pf1N1B4_2384 at 2701.874 kb on - strand, within Pf1N1B4_2384 at 2701.874 kb on - strand, within Pf1N1B4_2384 at 2701.874 kb on - strand, within Pf1N1B4_2384 at 2701.874 kb on - strand, within Pf1N1B4_2384 at 2701.874 kb on - strand, within Pf1N1B4_2384 at 2701.874 kb on - strand, within Pf1N1B4_2384 at 2701.894 kb on + strand, within Pf1N1B4_2384 at 2701.894 kb on + strand, within Pf1N1B4_2384 at 2701.894 kb on + strand, within Pf1N1B4_2384 at 2701.894 kb on + strand, within Pf1N1B4_2384 at 2701.895 kb on - strand, within Pf1N1B4_2384 at 2701.895 kb on - strand, within Pf1N1B4_2384 at 2701.895 kb on - strand, within Pf1N1B4_2384 at 2702.120 kb on + strand, within Pf1N1B4_2384 at 2702.120 kb on + strand, within Pf1N1B4_2384 at 2702.121 kb on - strand, within Pf1N1B4_2384 at 2702.143 kb on + strand, within Pf1N1B4_2384 at 2702.143 kb on + strand, within Pf1N1B4_2384 at 2702.143 kb on + strand, within Pf1N1B4_2384 at 2702.143 kb on + strand, within Pf1N1B4_2384 at 2702.143 kb on + strand, within Pf1N1B4_2384 at 2702.143 kb on + strand, within Pf1N1B4_2384 at 2702.144 kb on - strand, within Pf1N1B4_2384 at 2702.144 kb on - strand, within Pf1N1B4_2384 at 2702.144 kb on - strand, within Pf1N1B4_2384 at 2702.144 kb on - strand, within Pf1N1B4_2384 at 2702.144 kb on - strand, within Pf1N1B4_2384 at 2702.144 kb on - strand, within Pf1N1B4_2384 at 2702.144 kb on - strand, within Pf1N1B4_2384 at 2702.144 kb on - strand, within Pf1N1B4_2384 at 2702.144 kb on - strand, within Pf1N1B4_2384 at 2702.282 kb on - strand, within Pf1N1B4_2384 at 2702.387 kb on - strand at 2702.411 kb on + strand at 2702.412 kb on - strand at 2702.427 kb on + strand at 2702.427 kb on + strand at 2702.427 kb on + strand at 2702.427 kb on + strand at 2702.427 kb on + strand at 2702.428 kb on - strand at 2702.428 kb on - strand at 2702.496 kb on + strand at 2702.496 kb on + strand at 2702.496 kb on + strand at 2702.497 kb on - strand at 2702.497 kb on - strand at 2702.509 kb on + strand at 2702.509 kb on + strand at 2702.509 kb on + strand at 2702.509 kb on + strand at 2703.993 kb on - strand at 2703.993 kb on - strand at 2703.998 kb on - strand at 2703.998 kb on - strand at 2703.998 kb on - strand at 2703.998 kb on - strand at 2704.155 kb on - strand at 2704.165 kb on - strand at 2704.210 kb on + strand at 2704.222 kb on + strand at 2704.223 kb on - strand at 2704.237 kb on - strand at 2704.244 kb on + strand at 2704.277 kb on + strand at 2704.278 kb on - strand at 2704.300 kb on + strand at 2704.406 kb on + strand, within Pf1N1B4_2386 at 2704.406 kb on + strand, within Pf1N1B4_2386 at 2704.406 kb on + strand, within Pf1N1B4_2386 at 2704.406 kb on + strand, within Pf1N1B4_2386 at 2704.406 kb on + strand, within Pf1N1B4_2386 at 2704.406 kb on + strand, within Pf1N1B4_2386 at 2704.406 kb on + strand, within Pf1N1B4_2386 at 2704.407 kb on - strand, within Pf1N1B4_2386 at 2704.407 kb on - strand, within Pf1N1B4_2386 at 2704.407 kb on - strand, within Pf1N1B4_2386 at 2704.407 kb on - strand, within Pf1N1B4_2386 at 2704.448 kb on + strand, within Pf1N1B4_2386 at 2704.448 kb on + strand, within Pf1N1B4_2386 at 2704.448 kb on + strand, within Pf1N1B4_2386 at 2704.449 kb on - strand, within Pf1N1B4_2386 at 2704.449 kb on - strand, within Pf1N1B4_2386 at 2704.588 kb on + strand, within Pf1N1B4_2386 at 2704.664 kb on + strand, within Pf1N1B4_2386 at 2704.664 kb on + strand, within Pf1N1B4_2386 at 2704.840 kb on + strand at 2704.877 kb on + strand at 2704.878 kb on - strand at 2704.882 kb on - strand at 2704.910 kb on + strand at 2704.910 kb on + strand at 2704.910 kb on + strand at 2704.911 kb on - strand at 2704.911 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 2,701,621 + -1.4 2,701,622 - -0.8 2,701,622 - -1.6 2,701,721 - Pf1N1B4_2384 0.19 -3.6 2,701,795 + Pf1N1B4_2384 0.28 -2.0 2,701,795 + Pf1N1B4_2384 0.28 -3.1 2,701,795 + Pf1N1B4_2384 0.28 -0.0 2,701,795 + Pf1N1B4_2384 0.28 -2.9 2,701,795 + Pf1N1B4_2384 0.28 -1.4 2,701,795 + Pf1N1B4_2384 0.28 -0.9 2,701,795 + Pf1N1B4_2384 0.28 -0.8 2,701,795 + Pf1N1B4_2384 0.28 -0.8 2,701,796 - Pf1N1B4_2384 0.28 -1.9 2,701,796 - Pf1N1B4_2384 0.28 -3.0 2,701,796 - Pf1N1B4_2384 0.28 -3.2 2,701,796 - Pf1N1B4_2384 0.28 -3.0 2,701,796 - Pf1N1B4_2384 0.28 -1.8 2,701,796 - Pf1N1B4_2384 0.28 -0.9 2,701,796 - Pf1N1B4_2384 0.28 -3.4 2,701,867 + Pf1N1B4_2384 0.36 -2.7 2,701,867 + Pf1N1B4_2384 0.36 -0.3 2,701,868 - Pf1N1B4_2384 0.36 -0.6 2,701,868 - Pf1N1B4_2384 0.36 -2.4 2,701,868 - Pf1N1B4_2384 0.36 -1.4 2,701,868 - Pf1N1B4_2384 0.36 +0.4 2,701,868 - Pf1N1B4_2384 0.36 -1.3 2,701,873 + Pf1N1B4_2384 0.37 +0.2 2,701,873 + Pf1N1B4_2384 0.37 -3.0 2,701,873 + Pf1N1B4_2384 0.37 -0.9 2,701,873 + Pf1N1B4_2384 0.37 -1.7 2,701,873 + Pf1N1B4_2384 0.37 -2.1 2,701,873 + Pf1N1B4_2384 0.37 -0.3 2,701,873 + Pf1N1B4_2384 0.37 -1.7 2,701,873 + Pf1N1B4_2384 0.37 +0.6 2,701,873 + Pf1N1B4_2384 0.37 -2.3 2,701,873 + Pf1N1B4_2384 0.37 -2.4 2,701,873 + Pf1N1B4_2384 0.37 -3.6 2,701,873 + Pf1N1B4_2384 0.37 -1.5 2,701,873 + Pf1N1B4_2384 0.37 +0.1 2,701,873 + Pf1N1B4_2384 0.37 -1.5 2,701,874 - Pf1N1B4_2384 0.37 -0.4 2,701,874 - Pf1N1B4_2384 0.37 -1.6 2,701,874 - Pf1N1B4_2384 0.37 -1.6 2,701,874 - Pf1N1B4_2384 0.37 -1.9 2,701,874 - Pf1N1B4_2384 0.37 -1.1 2,701,874 - Pf1N1B4_2384 0.37 -0.6 2,701,874 - Pf1N1B4_2384 0.37 -0.3 2,701,874 - Pf1N1B4_2384 0.37 -1.7 2,701,874 - Pf1N1B4_2384 0.37 -1.0 2,701,894 + Pf1N1B4_2384 0.39 -2.2 2,701,894 + Pf1N1B4_2384 0.39 -1.2 2,701,894 + Pf1N1B4_2384 0.39 -2.2 2,701,894 + Pf1N1B4_2384 0.39 -1.8 2,701,895 - Pf1N1B4_2384 0.40 -1.1 2,701,895 - Pf1N1B4_2384 0.40 -0.2 2,701,895 - Pf1N1B4_2384 0.40 -3.5 2,702,120 + Pf1N1B4_2384 0.66 -2.7 2,702,120 + Pf1N1B4_2384 0.66 -1.2 2,702,121 - Pf1N1B4_2384 0.66 -0.6 2,702,143 + Pf1N1B4_2384 0.69 -1.2 2,702,143 + Pf1N1B4_2384 0.69 -1.7 2,702,143 + Pf1N1B4_2384 0.69 -0.4 2,702,143 + Pf1N1B4_2384 0.69 -2.1 2,702,143 + Pf1N1B4_2384 0.69 -1.5 2,702,143 + Pf1N1B4_2384 0.69 -2.4 2,702,144 - Pf1N1B4_2384 0.69 -3.2 2,702,144 - Pf1N1B4_2384 0.69 -2.4 2,702,144 - Pf1N1B4_2384 0.69 -1.8 2,702,144 - Pf1N1B4_2384 0.69 -1.7 2,702,144 - Pf1N1B4_2384 0.69 -0.7 2,702,144 - Pf1N1B4_2384 0.69 -3.3 2,702,144 - Pf1N1B4_2384 0.69 -3.0 2,702,144 - Pf1N1B4_2384 0.69 -1.5 2,702,144 - Pf1N1B4_2384 0.69 -1.4 2,702,282 - Pf1N1B4_2384 0.85 -2.7 2,702,387 - -2.3 2,702,411 + -1.0 2,702,412 - +0.2 2,702,427 + -2.2 2,702,427 + -2.6 2,702,427 + -0.9 2,702,427 + -0.7 2,702,427 + -1.6 2,702,428 - -0.5 2,702,428 - +0.6 2,702,496 + +0.5 2,702,496 + -2.4 2,702,496 + -2.6 2,702,497 - +1.9 2,702,497 - +0.6 2,702,509 + -1.0 2,702,509 + -1.7 2,702,509 + -2.2 2,702,509 + -1.1 2,703,993 - -0.4 2,703,993 - -0.9 2,703,998 - +0.4 2,703,998 - +0.8 2,703,998 - +0.2 2,703,998 - +0.2 2,704,155 - -0.4 2,704,165 - +0.7 2,704,210 + -0.6 2,704,222 + -0.6 2,704,223 - -1.6 2,704,237 - +0.3 2,704,244 + -1.7 2,704,277 + +0.2 2,704,278 - -0.3 2,704,300 + -1.0 2,704,406 + Pf1N1B4_2386 0.26 +1.6 2,704,406 + Pf1N1B4_2386 0.26 +0.9 2,704,406 + Pf1N1B4_2386 0.26 -0.5 2,704,406 + Pf1N1B4_2386 0.26 -0.5 2,704,406 + Pf1N1B4_2386 0.26 +0.3 2,704,406 + Pf1N1B4_2386 0.26 -1.3 2,704,406 + Pf1N1B4_2386 0.26 +1.2 2,704,407 - Pf1N1B4_2386 0.26 +0.4 2,704,407 - Pf1N1B4_2386 0.26 -0.6 2,704,407 - Pf1N1B4_2386 0.26 -0.5 2,704,407 - Pf1N1B4_2386 0.26 -0.7 2,704,448 + Pf1N1B4_2386 0.36 +0.7 2,704,448 + Pf1N1B4_2386 0.36 +0.1 2,704,448 + Pf1N1B4_2386 0.36 +0.0 2,704,449 - Pf1N1B4_2386 0.37 +2.9 2,704,449 - Pf1N1B4_2386 0.37 -0.3 2,704,588 + Pf1N1B4_2386 0.71 +0.7 2,704,664 + Pf1N1B4_2386 0.90 -1.3 2,704,664 + Pf1N1B4_2386 0.90 -0.1 2,704,840 + +0.1 2,704,877 + +1.3 2,704,878 - -2.7 2,704,882 - -0.7 2,704,910 + +3.5 2,704,910 + -0.8 2,704,910 + -1.0 2,704,911 - -0.5 2,704,911 - +0.3
Or see this region's nucleotide sequence