Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2385

Experiment: nitrogen source nitrite 0.5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2384 and Pf1N1B4_2385 are separated by 160 nucleotidesPf1N1B4_2385 and Pf1N1B4_2386 are separated by 325 nucleotidesPf1N1B4_2386 and Pf1N1B4_2387 are separated by 205 nucleotides Pf1N1B4_2384 - 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20), at 2,701,560 to 2,702,405 _2384 Pf1N1B4_2385 - Adenosylhomocysteinase (EC 3.3.1.1), at 2,702,566 to 2,703,975 _2385 Pf1N1B4_2386 - cytosolic long-chain acyl-CoA thioester hydrolase family protein, at 2,704,301 to 2,704,705 _2386 Pf1N1B4_2387 - Putative transport, at 2,704,911 to 2,705,798 _2387 Position (kb) 2702 2703 2704Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2701.621 kb on + strandat 2701.622 kb on - strandat 2701.622 kb on - strandat 2701.721 kb on - strand, within Pf1N1B4_2384at 2701.795 kb on + strand, within Pf1N1B4_2384at 2701.795 kb on + strand, within Pf1N1B4_2384at 2701.795 kb on + strand, within Pf1N1B4_2384at 2701.795 kb on + strand, within Pf1N1B4_2384at 2701.795 kb on + strand, within Pf1N1B4_2384at 2701.795 kb on + strand, within Pf1N1B4_2384at 2701.795 kb on + strand, within Pf1N1B4_2384at 2701.795 kb on + strand, within Pf1N1B4_2384at 2701.796 kb on - strand, within Pf1N1B4_2384at 2701.796 kb on - strand, within Pf1N1B4_2384at 2701.796 kb on - strand, within Pf1N1B4_2384at 2701.796 kb on - strand, within Pf1N1B4_2384at 2701.796 kb on - strand, within Pf1N1B4_2384at 2701.796 kb on - strand, within Pf1N1B4_2384at 2701.796 kb on - strand, within Pf1N1B4_2384at 2701.867 kb on + strand, within Pf1N1B4_2384at 2701.867 kb on + strand, within Pf1N1B4_2384at 2701.868 kb on - strand, within Pf1N1B4_2384at 2701.868 kb on - strand, within Pf1N1B4_2384at 2701.868 kb on - strand, within Pf1N1B4_2384at 2701.868 kb on - strand, within Pf1N1B4_2384at 2701.868 kb on - strand, within Pf1N1B4_2384at 2701.873 kb on + strand, within Pf1N1B4_2384at 2701.873 kb on + strand, within Pf1N1B4_2384at 2701.873 kb on + strand, within Pf1N1B4_2384at 2701.873 kb on + strand, within Pf1N1B4_2384at 2701.873 kb on + strand, within Pf1N1B4_2384at 2701.873 kb on + strand, within Pf1N1B4_2384at 2701.873 kb on + strand, within Pf1N1B4_2384at 2701.873 kb on + strand, within Pf1N1B4_2384at 2701.873 kb on + strand, within Pf1N1B4_2384at 2701.873 kb on + strand, within Pf1N1B4_2384at 2701.873 kb on + strand, within Pf1N1B4_2384at 2701.873 kb on + strand, within Pf1N1B4_2384at 2701.873 kb on + strand, within Pf1N1B4_2384at 2701.873 kb on + strand, within Pf1N1B4_2384at 2701.874 kb on - strand, within Pf1N1B4_2384at 2701.874 kb on - strand, within Pf1N1B4_2384at 2701.874 kb on - strand, within Pf1N1B4_2384at 2701.874 kb on - strand, within Pf1N1B4_2384at 2701.874 kb on - strand, within Pf1N1B4_2384at 2701.874 kb on - strand, within Pf1N1B4_2384at 2701.874 kb on - strand, within Pf1N1B4_2384at 2701.874 kb on - strand, within Pf1N1B4_2384at 2701.874 kb on - strand, within Pf1N1B4_2384at 2701.894 kb on + strand, within Pf1N1B4_2384at 2701.894 kb on + strand, within Pf1N1B4_2384at 2701.894 kb on + strand, within Pf1N1B4_2384at 2701.894 kb on + strand, within Pf1N1B4_2384at 2701.895 kb on - strand, within Pf1N1B4_2384at 2701.895 kb on - strand, within Pf1N1B4_2384at 2701.895 kb on - strand, within Pf1N1B4_2384at 2702.120 kb on + strand, within Pf1N1B4_2384at 2702.120 kb on + strand, within Pf1N1B4_2384at 2702.121 kb on - strand, within Pf1N1B4_2384at 2702.143 kb on + strand, within Pf1N1B4_2384at 2702.143 kb on + strand, within Pf1N1B4_2384at 2702.143 kb on + strand, within Pf1N1B4_2384at 2702.143 kb on + strand, within Pf1N1B4_2384at 2702.143 kb on + strand, within Pf1N1B4_2384at 2702.143 kb on + strand, within Pf1N1B4_2384at 2702.144 kb on - strand, within Pf1N1B4_2384at 2702.144 kb on - strand, within Pf1N1B4_2384at 2702.144 kb on - strand, within Pf1N1B4_2384at 2702.144 kb on - strand, within Pf1N1B4_2384at 2702.144 kb on - strand, within Pf1N1B4_2384at 2702.144 kb on - strand, within Pf1N1B4_2384at 2702.144 kb on - strand, within Pf1N1B4_2384at 2702.144 kb on - strand, within Pf1N1B4_2384at 2702.144 kb on - strand, within Pf1N1B4_2384at 2702.282 kb on - strand, within Pf1N1B4_2384at 2702.387 kb on - strandat 2702.411 kb on + strandat 2702.412 kb on - strandat 2702.427 kb on + strandat 2702.427 kb on + strandat 2702.427 kb on + strandat 2702.427 kb on + strandat 2702.427 kb on + strandat 2702.428 kb on - strandat 2702.428 kb on - strandat 2702.496 kb on + strandat 2702.496 kb on + strandat 2702.496 kb on + strandat 2702.497 kb on - strandat 2702.497 kb on - strandat 2702.509 kb on + strandat 2702.509 kb on + strandat 2702.509 kb on + strandat 2702.509 kb on + strandat 2703.993 kb on - strandat 2703.993 kb on - strandat 2703.998 kb on - strandat 2703.998 kb on - strandat 2703.998 kb on - strandat 2703.998 kb on - strandat 2704.155 kb on - strandat 2704.165 kb on - strandat 2704.210 kb on + strandat 2704.222 kb on + strandat 2704.223 kb on - strandat 2704.237 kb on - strandat 2704.244 kb on + strandat 2704.277 kb on + strandat 2704.278 kb on - strandat 2704.300 kb on + strandat 2704.406 kb on + strand, within Pf1N1B4_2386at 2704.406 kb on + strand, within Pf1N1B4_2386at 2704.406 kb on + strand, within Pf1N1B4_2386at 2704.406 kb on + strand, within Pf1N1B4_2386at 2704.406 kb on + strand, within Pf1N1B4_2386at 2704.406 kb on + strand, within Pf1N1B4_2386at 2704.406 kb on + strand, within Pf1N1B4_2386at 2704.407 kb on - strand, within Pf1N1B4_2386at 2704.407 kb on - strand, within Pf1N1B4_2386at 2704.407 kb on - strand, within Pf1N1B4_2386at 2704.407 kb on - strand, within Pf1N1B4_2386at 2704.448 kb on + strand, within Pf1N1B4_2386at 2704.448 kb on + strand, within Pf1N1B4_2386at 2704.448 kb on + strand, within Pf1N1B4_2386at 2704.449 kb on - strand, within Pf1N1B4_2386at 2704.449 kb on - strand, within Pf1N1B4_2386at 2704.588 kb on + strand, within Pf1N1B4_2386at 2704.664 kb on + strand, within Pf1N1B4_2386at 2704.664 kb on + strand, within Pf1N1B4_2386at 2704.840 kb on + strandat 2704.877 kb on + strandat 2704.878 kb on - strandat 2704.882 kb on - strandat 2704.910 kb on + strandat 2704.910 kb on + strandat 2704.910 kb on + strandat 2704.911 kb on - strandat 2704.911 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM
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2,701,621 + -1.4
2,701,622 - -0.8
2,701,622 - -1.6
2,701,721 - Pf1N1B4_2384 0.19 -3.6
2,701,795 + Pf1N1B4_2384 0.28 -2.0
2,701,795 + Pf1N1B4_2384 0.28 -3.1
2,701,795 + Pf1N1B4_2384 0.28 -0.0
2,701,795 + Pf1N1B4_2384 0.28 -2.9
2,701,795 + Pf1N1B4_2384 0.28 -1.4
2,701,795 + Pf1N1B4_2384 0.28 -0.9
2,701,795 + Pf1N1B4_2384 0.28 -0.8
2,701,795 + Pf1N1B4_2384 0.28 -0.8
2,701,796 - Pf1N1B4_2384 0.28 -1.9
2,701,796 - Pf1N1B4_2384 0.28 -3.0
2,701,796 - Pf1N1B4_2384 0.28 -3.2
2,701,796 - Pf1N1B4_2384 0.28 -3.0
2,701,796 - Pf1N1B4_2384 0.28 -1.8
2,701,796 - Pf1N1B4_2384 0.28 -0.9
2,701,796 - Pf1N1B4_2384 0.28 -3.4
2,701,867 + Pf1N1B4_2384 0.36 -2.7
2,701,867 + Pf1N1B4_2384 0.36 -0.3
2,701,868 - Pf1N1B4_2384 0.36 -0.6
2,701,868 - Pf1N1B4_2384 0.36 -2.4
2,701,868 - Pf1N1B4_2384 0.36 -1.4
2,701,868 - Pf1N1B4_2384 0.36 +0.4
2,701,868 - Pf1N1B4_2384 0.36 -1.3
2,701,873 + Pf1N1B4_2384 0.37 +0.2
2,701,873 + Pf1N1B4_2384 0.37 -3.0
2,701,873 + Pf1N1B4_2384 0.37 -0.9
2,701,873 + Pf1N1B4_2384 0.37 -1.7
2,701,873 + Pf1N1B4_2384 0.37 -2.1
2,701,873 + Pf1N1B4_2384 0.37 -0.3
2,701,873 + Pf1N1B4_2384 0.37 -1.7
2,701,873 + Pf1N1B4_2384 0.37 +0.6
2,701,873 + Pf1N1B4_2384 0.37 -2.3
2,701,873 + Pf1N1B4_2384 0.37 -2.4
2,701,873 + Pf1N1B4_2384 0.37 -3.6
2,701,873 + Pf1N1B4_2384 0.37 -1.5
2,701,873 + Pf1N1B4_2384 0.37 +0.1
2,701,873 + Pf1N1B4_2384 0.37 -1.5
2,701,874 - Pf1N1B4_2384 0.37 -0.4
2,701,874 - Pf1N1B4_2384 0.37 -1.6
2,701,874 - Pf1N1B4_2384 0.37 -1.6
2,701,874 - Pf1N1B4_2384 0.37 -1.9
2,701,874 - Pf1N1B4_2384 0.37 -1.1
2,701,874 - Pf1N1B4_2384 0.37 -0.6
2,701,874 - Pf1N1B4_2384 0.37 -0.3
2,701,874 - Pf1N1B4_2384 0.37 -1.7
2,701,874 - Pf1N1B4_2384 0.37 -1.0
2,701,894 + Pf1N1B4_2384 0.39 -2.2
2,701,894 + Pf1N1B4_2384 0.39 -1.2
2,701,894 + Pf1N1B4_2384 0.39 -2.2
2,701,894 + Pf1N1B4_2384 0.39 -1.8
2,701,895 - Pf1N1B4_2384 0.40 -1.1
2,701,895 - Pf1N1B4_2384 0.40 -0.2
2,701,895 - Pf1N1B4_2384 0.40 -3.5
2,702,120 + Pf1N1B4_2384 0.66 -2.7
2,702,120 + Pf1N1B4_2384 0.66 -1.2
2,702,121 - Pf1N1B4_2384 0.66 -0.6
2,702,143 + Pf1N1B4_2384 0.69 -1.2
2,702,143 + Pf1N1B4_2384 0.69 -1.7
2,702,143 + Pf1N1B4_2384 0.69 -0.4
2,702,143 + Pf1N1B4_2384 0.69 -2.1
2,702,143 + Pf1N1B4_2384 0.69 -1.5
2,702,143 + Pf1N1B4_2384 0.69 -2.4
2,702,144 - Pf1N1B4_2384 0.69 -3.2
2,702,144 - Pf1N1B4_2384 0.69 -2.4
2,702,144 - Pf1N1B4_2384 0.69 -1.8
2,702,144 - Pf1N1B4_2384 0.69 -1.7
2,702,144 - Pf1N1B4_2384 0.69 -0.7
2,702,144 - Pf1N1B4_2384 0.69 -3.3
2,702,144 - Pf1N1B4_2384 0.69 -3.0
2,702,144 - Pf1N1B4_2384 0.69 -1.5
2,702,144 - Pf1N1B4_2384 0.69 -1.4
2,702,282 - Pf1N1B4_2384 0.85 -2.7
2,702,387 - -2.3
2,702,411 + -1.0
2,702,412 - +0.2
2,702,427 + -2.2
2,702,427 + -2.6
2,702,427 + -0.9
2,702,427 + -0.7
2,702,427 + -1.6
2,702,428 - -0.5
2,702,428 - +0.6
2,702,496 + +0.5
2,702,496 + -2.4
2,702,496 + -2.6
2,702,497 - +1.9
2,702,497 - +0.6
2,702,509 + -1.0
2,702,509 + -1.7
2,702,509 + -2.2
2,702,509 + -1.1
2,703,993 - -0.4
2,703,993 - -0.9
2,703,998 - +0.4
2,703,998 - +0.8
2,703,998 - +0.2
2,703,998 - +0.2
2,704,155 - -0.4
2,704,165 - +0.7
2,704,210 + -0.6
2,704,222 + -0.6
2,704,223 - -1.6
2,704,237 - +0.3
2,704,244 + -1.7
2,704,277 + +0.2
2,704,278 - -0.3
2,704,300 + -1.0
2,704,406 + Pf1N1B4_2386 0.26 +1.6
2,704,406 + Pf1N1B4_2386 0.26 +0.9
2,704,406 + Pf1N1B4_2386 0.26 -0.5
2,704,406 + Pf1N1B4_2386 0.26 -0.5
2,704,406 + Pf1N1B4_2386 0.26 +0.3
2,704,406 + Pf1N1B4_2386 0.26 -1.3
2,704,406 + Pf1N1B4_2386 0.26 +1.2
2,704,407 - Pf1N1B4_2386 0.26 +0.4
2,704,407 - Pf1N1B4_2386 0.26 -0.6
2,704,407 - Pf1N1B4_2386 0.26 -0.5
2,704,407 - Pf1N1B4_2386 0.26 -0.7
2,704,448 + Pf1N1B4_2386 0.36 +0.7
2,704,448 + Pf1N1B4_2386 0.36 +0.1
2,704,448 + Pf1N1B4_2386 0.36 +0.0
2,704,449 - Pf1N1B4_2386 0.37 +2.9
2,704,449 - Pf1N1B4_2386 0.37 -0.3
2,704,588 + Pf1N1B4_2386 0.71 +0.7
2,704,664 + Pf1N1B4_2386 0.90 -1.3
2,704,664 + Pf1N1B4_2386 0.90 -0.1
2,704,840 + +0.1
2,704,877 + +1.3
2,704,878 - -2.7
2,704,882 - -0.7
2,704,910 + +3.5
2,704,910 + -0.8
2,704,910 + -1.0
2,704,911 - -0.5
2,704,911 - +0.3

Or see this region's nucleotide sequence