Experiment: nitrogen source nitrite 0.5mM 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt Pf1N1B4_2012 and Pf1N1B4_2013 are separated by 47 nucleotides Pf1N1B4_2013 and Pf1N1B4_2014 are separated by 111 nucleotides Pf1N1B4_2014 and Pf1N1B4_2015 are separated by 99 nucleotides Pf1N1B4_2015 and Pf1N1B4_2016 are separated by 17 nucleotides  
        
        Pf1N1B4_2012 - Amino acid ABC transporter, permease protein, at 2,298,879 to 2,299,661 
         
        _2012 
         
        
        Pf1N1B4_2013 - Lysine-arginine-ornithine-binding periplasmic protein precursor (TC 3.A.1.3.1), at 2,299,709 to 2,300,530 
         
        _2013 
         
        
        Pf1N1B4_2014 - Homocysteine S-methyltransferase (EC 2.1.1.10), at 2,300,642 to 2,301,541 
         
        _2014 
         
        
        Pf1N1B4_2015 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 2,301,641 to 2,302,309 
         
        _2015 
         
        
        Pf1N1B4_2016 - N-acetylglutamate synthase (EC 2.3.1.1), at 2,302,327 to 2,302,773 
         
        _2016 
         Position (kb)  
2300 
 
2301 
 
2302 Strain fitness (log2 ratio)  
-3 
 
-2 
 
-1 
 
0 
 
1 
 
2 at 2299.851 kb on + strand at 2299.989 kb on + strand, within Pf1N1B4_2013 at 2299.989 kb on + strand, within Pf1N1B4_2013 at 2300.070 kb on + strand, within Pf1N1B4_2013 at 2300.079 kb on + strand, within Pf1N1B4_2013 at 2300.079 kb on + strand, within Pf1N1B4_2013 at 2300.079 kb on + strand, within Pf1N1B4_2013 at 2300.079 kb on + strand, within Pf1N1B4_2013 at 2300.079 kb on + strand, within Pf1N1B4_2013 at 2300.080 kb on - strand, within Pf1N1B4_2013 at 2300.080 kb on - strand, within Pf1N1B4_2013 at 2300.137 kb on + strand, within Pf1N1B4_2013 at 2300.137 kb on + strand, within Pf1N1B4_2013 at 2300.137 kb on + strand, within Pf1N1B4_2013 at 2300.137 kb on + strand, within Pf1N1B4_2013 at 2300.137 kb on + strand at 2300.137 kb on + strand, within Pf1N1B4_2013 at 2300.138 kb on - strand, within Pf1N1B4_2013 at 2300.138 kb on - strand, within Pf1N1B4_2013 at 2300.138 kb on - strand, within Pf1N1B4_2013 at 2300.138 kb on - strand, within Pf1N1B4_2013 at 2300.181 kb on + strand, within Pf1N1B4_2013 at 2300.181 kb on + strand, within Pf1N1B4_2013 at 2300.181 kb on + strand, within Pf1N1B4_2013 at 2300.181 kb on + strand, within Pf1N1B4_2013 at 2300.182 kb on - strand, within Pf1N1B4_2013 at 2300.182 kb on - strand, within Pf1N1B4_2013 at 2300.184 kb on + strand, within Pf1N1B4_2013 at 2300.184 kb on + strand, within Pf1N1B4_2013 at 2300.184 kb on + strand, within Pf1N1B4_2013 at 2300.184 kb on + strand, within Pf1N1B4_2013 at 2300.185 kb on - strand, within Pf1N1B4_2013 at 2300.185 kb on - strand, within Pf1N1B4_2013 at 2300.351 kb on + strand, within Pf1N1B4_2013 at 2300.404 kb on - strand, within Pf1N1B4_2013 at 2300.580 kb on - strand at 2300.587 kb on + strand at 2300.587 kb on + strand at 2300.587 kb on + strand at 2300.588 kb on - strand at 2300.589 kb on + strand at 2300.590 kb on - strand at 2300.590 kb on - strand at 2300.783 kb on + strand, within Pf1N1B4_2014 at 2300.783 kb on + strand, within Pf1N1B4_2014 at 2300.783 kb on + strand, within Pf1N1B4_2014 at 2300.784 kb on - strand, within Pf1N1B4_2014 at 2300.784 kb on - strand, within Pf1N1B4_2014 at 2300.784 kb on - strand, within Pf1N1B4_2014 at 2300.785 kb on + strand, within Pf1N1B4_2014 at 2300.785 kb on + strand, within Pf1N1B4_2014 at 2300.786 kb on - strand, within Pf1N1B4_2014 at 2300.822 kb on + strand, within Pf1N1B4_2014 at 2300.843 kb on - strand, within Pf1N1B4_2014 at 2300.843 kb on - strand, within Pf1N1B4_2014 at 2301.434 kb on + strand, within Pf1N1B4_2014 at 2301.511 kb on + strand at 2301.511 kb on + strand at 2301.511 kb on + strand at 2301.512 kb on - strand at 2301.512 kb on - strand at 2301.512 kb on - strand at 2301.579 kb on + strand at 2301.579 kb on + strand at 2301.579 kb on + strand at 2301.579 kb on + strand at 2301.579 kb on + strand at 2301.580 kb on - strand at 2301.601 kb on + strand at 2301.601 kb on + strand at 2301.602 kb on - strand at 2301.602 kb on - strand at 2301.602 kb on - strand at 2301.632 kb on - strand at 2301.632 kb on - strand at 2301.840 kb on + strand, within Pf1N1B4_2015 at 2301.840 kb on + strand, within Pf1N1B4_2015 at 2301.841 kb on - strand, within Pf1N1B4_2015 at 2301.841 kb on - strand, within Pf1N1B4_2015 at 2301.921 kb on + strand, within Pf1N1B4_2015 at 2302.071 kb on + strand, within Pf1N1B4_2015 at 2302.071 kb on + strand, within Pf1N1B4_2015 at 2302.072 kb on - strand, within Pf1N1B4_2015 at 2302.072 kb on - strand, within Pf1N1B4_2015 at 2302.149 kb on + strand, within Pf1N1B4_2015 at 2302.149 kb on + strand, within Pf1N1B4_2015 at 2302.150 kb on - strand, within Pf1N1B4_2015 at 2302.328 kb on + strand at 2302.329 kb on - strand at 2302.329 kb on - strand at 2302.329 kb on - strand at 2302.329 kb on - strand at 2302.329 kb on - strand at 2302.329 kb on - strand at 2302.329 kb on - strand at 2302.329 kb on - strand at 2302.330 kb on + strand at 2302.330 kb on + strand at 2302.330 kb on + strand at 2302.330 kb on + strand at 2302.331 kb on - strand at 2302.331 kb on - strand at 2302.331 kb on - strand at 2302.331 kb on - strand at 2302.331 kb on - strand at 2302.331 kb on - strand at 2302.331 kb on - strand at 2302.331 kb on - strand at 2302.331 kb on - strand at 2302.379 kb on + strand, within Pf1N1B4_2016 at 2302.380 kb on - strand, within Pf1N1B4_2016 at 2302.380 kb on - strand, within Pf1N1B4_2016 at 2302.380 kb on - strand, within Pf1N1B4_2016 at 2302.458 kb on - strand, within Pf1N1B4_2016 at 2302.458 kb on - strand, within Pf1N1B4_2016 at 2302.529 kb on + strand, within Pf1N1B4_2016 at 2302.530 kb on - strand, within Pf1N1B4_2016 at 2302.530 kb on - strand, within Pf1N1B4_2016 at 2302.530 kb on - strand, within Pf1N1B4_2016  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   nitrogen source nitrite 0.5mM             remove   2,299,851   +        -0.1   2,299,989   +    Pf1N1B4_2013   0.34  +0.4   2,299,989   +    Pf1N1B4_2013   0.34  +0.1   2,300,070   +    Pf1N1B4_2013   0.44  +1.4   2,300,079   +    Pf1N1B4_2013   0.45  -0.1   2,300,079   +    Pf1N1B4_2013   0.45  +1.4   2,300,079   +    Pf1N1B4_2013   0.45  +1.7   2,300,079   +    Pf1N1B4_2013   0.45  -0.9   2,300,079   +    Pf1N1B4_2013   0.45  +0.2   2,300,080   -    Pf1N1B4_2013   0.45  -0.9   2,300,080   -    Pf1N1B4_2013   0.45  +0.1   2,300,137   +    Pf1N1B4_2013   0.52  +1.1   2,300,137   +    Pf1N1B4_2013   0.52  +1.3   2,300,137   +    Pf1N1B4_2013   0.52  -2.1   2,300,137   +    Pf1N1B4_2013   0.52  +0.3   2,300,137   +        -0.4   2,300,137   +    Pf1N1B4_2013   0.52  -1.4   2,300,138   -    Pf1N1B4_2013   0.52  +0.2   2,300,138   -    Pf1N1B4_2013   0.52  +0.2   2,300,138   -    Pf1N1B4_2013   0.52  +0.9   2,300,138   -    Pf1N1B4_2013   0.52  -0.7   2,300,181   +    Pf1N1B4_2013   0.57  -1.2   2,300,181   +    Pf1N1B4_2013   0.57  +0.3   2,300,181   +    Pf1N1B4_2013   0.57  +1.2   2,300,181   +    Pf1N1B4_2013   0.57  +0.8   2,300,182   -    Pf1N1B4_2013   0.58  +0.6   2,300,182   -    Pf1N1B4_2013   0.58  +0.4   2,300,184   +    Pf1N1B4_2013   0.58  -1.4   2,300,184   +    Pf1N1B4_2013   0.58  -0.7   2,300,184   +    Pf1N1B4_2013   0.58  +0.7   2,300,184   +    Pf1N1B4_2013   0.58  +1.1   2,300,185   -    Pf1N1B4_2013   0.58  +2.1   2,300,185   -    Pf1N1B4_2013   0.58  +0.4   2,300,351   +    Pf1N1B4_2013   0.78  +0.8   2,300,404   -    Pf1N1B4_2013   0.85  -0.4   2,300,580   -        +0.3   2,300,587   +        -0.5   2,300,587   +        +0.3   2,300,587   +        +0.8   2,300,588   -        -0.6   2,300,589   +        +0.9   2,300,590   -        -1.1   2,300,590   -        -0.8   2,300,783   +    Pf1N1B4_2014   0.16  +1.5   2,300,783   +    Pf1N1B4_2014   0.16  -0.1   2,300,783   +    Pf1N1B4_2014   0.16  +0.1   2,300,784   -    Pf1N1B4_2014   0.16  +0.1   2,300,784   -    Pf1N1B4_2014   0.16  -1.2   2,300,784   -    Pf1N1B4_2014   0.16  +0.1   2,300,785   +    Pf1N1B4_2014   0.16  +1.4   2,300,785   +    Pf1N1B4_2014   0.16  +1.1   2,300,786   -    Pf1N1B4_2014   0.16  +1.0   2,300,822   +    Pf1N1B4_2014   0.20  -0.0   2,300,843   -    Pf1N1B4_2014   0.22  +0.9   2,300,843   -    Pf1N1B4_2014   0.22  -0.1   2,301,434   +    Pf1N1B4_2014   0.88  +1.9   2,301,511   +        +0.5   2,301,511   +        +0.7   2,301,511   +        -1.2   2,301,512   -        +1.6   2,301,512   -        -0.7   2,301,512   -        +1.1   2,301,579   +        -1.8   2,301,579   +        -0.4   2,301,579   +        -2.2   2,301,579   +        -1.2   2,301,579   +        -0.9   2,301,580   -        -0.7   2,301,601   +        +0.3   2,301,601   +        +0.5   2,301,602   -        -1.2   2,301,602   -        +1.1   2,301,602   -        +1.9   2,301,632   -        +0.8   2,301,632   -        -0.7   2,301,840   +    Pf1N1B4_2015   0.30  -1.1   2,301,840   +    Pf1N1B4_2015   0.30  -1.6   2,301,841   -    Pf1N1B4_2015   0.30  +0.3   2,301,841   -    Pf1N1B4_2015   0.30  +0.2   2,301,921   +    Pf1N1B4_2015   0.42  +0.8   2,302,071   +    Pf1N1B4_2015   0.64  -1.0   2,302,071   +    Pf1N1B4_2015   0.64  +0.6   2,302,072   -    Pf1N1B4_2015   0.64  +0.6   2,302,072   -    Pf1N1B4_2015   0.64  +0.8   2,302,149   +    Pf1N1B4_2015   0.76  -2.4   2,302,149   +    Pf1N1B4_2015   0.76  +0.5   2,302,150   -    Pf1N1B4_2015   0.76  +1.4   2,302,328   +        +1.2   2,302,329   -        +0.1   2,302,329   -        -2.3   2,302,329   -        -1.2   2,302,329   -        +0.5   2,302,329   -        -0.2   2,302,329   -        +0.9   2,302,329   -        +0.1   2,302,329   -        -0.4   2,302,330   +        -1.7   2,302,330   +        +0.2   2,302,330   +        -0.7   2,302,330   +        -0.5   2,302,331   -        +0.9   2,302,331   -        +1.0   2,302,331   -        +0.6   2,302,331   -        -0.6   2,302,331   -        -1.9   2,302,331   -        +0.2   2,302,331   -        -1.0   2,302,331   -        -0.8   2,302,331   -        -0.4   2,302,379   +    Pf1N1B4_2016   0.12  -1.7   2,302,380   -    Pf1N1B4_2016   0.12  -1.0   2,302,380   -    Pf1N1B4_2016   0.12  -0.8   2,302,380   -    Pf1N1B4_2016   0.12  +0.1   2,302,458   -    Pf1N1B4_2016   0.29  -0.9   2,302,458   -    Pf1N1B4_2016   0.29  -0.3   2,302,529   +    Pf1N1B4_2016   0.45  -1.3   2,302,530   -    Pf1N1B4_2016   0.45  +0.5   2,302,530   -    Pf1N1B4_2016   0.45  -2.9   2,302,530   -    Pf1N1B4_2016   0.45  -0.8 
 
Or see this region's nucleotide sequence