Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1699 and Pf1N1B4_1700 overlap by 4 nucleotides Pf1N1B4_1700 and Pf1N1B4_1701 overlap by 24 nucleotides Pf1N1B4_1701 and Pf1N1B4_1702 are separated by 2 nucleotides Pf1N1B4_1702 and Pf1N1B4_1703 overlap by 11 nucleotides
Pf1N1B4_1699 - Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases, at 1,946,300 to 1,947,658
_1699
Pf1N1B4_1700 - hypothetical protein, at 1,947,655 to 1,947,828
_1700
Pf1N1B4_1701 - Methionine ABC transporter substrate-binding protein, at 1,947,805 to 1,948,614
_1701
Pf1N1B4_1702 - Methionine ABC transporter ATP-binding protein, at 1,948,617 to 1,949,738
_1702
Pf1N1B4_1703 - Methionine ABC transporter permease protein, at 1,949,728 to 1,950,372
_1703
Position (kb)
1948
1949
1950 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1947.635 kb on - strand at 1947.783 kb on + strand, within Pf1N1B4_1700 at 1947.944 kb on + strand, within Pf1N1B4_1701 at 1947.944 kb on + strand, within Pf1N1B4_1701 at 1947.945 kb on - strand, within Pf1N1B4_1701 at 1948.045 kb on + strand, within Pf1N1B4_1701 at 1948.045 kb on + strand, within Pf1N1B4_1701 at 1948.046 kb on - strand, within Pf1N1B4_1701 at 1948.153 kb on + strand, within Pf1N1B4_1701 at 1948.153 kb on + strand, within Pf1N1B4_1701 at 1948.363 kb on + strand, within Pf1N1B4_1701 at 1948.399 kb on + strand, within Pf1N1B4_1701 at 1948.399 kb on + strand, within Pf1N1B4_1701 at 1948.400 kb on - strand, within Pf1N1B4_1701 at 1948.400 kb on - strand, within Pf1N1B4_1701 at 1948.469 kb on - strand, within Pf1N1B4_1701 at 1948.469 kb on - strand, within Pf1N1B4_1701 at 1948.469 kb on - strand, within Pf1N1B4_1701 at 1948.537 kb on + strand at 1948.537 kb on + strand at 1948.537 kb on + strand at 1948.538 kb on - strand at 1948.538 kb on - strand at 1948.538 kb on - strand at 1948.538 kb on - strand at 1948.538 kb on - strand at 1948.538 kb on - strand at 1948.538 kb on - strand at 1948.592 kb on - strand at 1948.661 kb on + strand at 1948.661 kb on + strand at 1948.734 kb on + strand, within Pf1N1B4_1702 at 1948.735 kb on - strand, within Pf1N1B4_1702 at 1948.735 kb on - strand, within Pf1N1B4_1702 at 1949.727 kb on + strand at 1949.727 kb on + strand at 1949.727 kb on + strand at 1949.727 kb on + strand at 1949.728 kb on - strand at 1949.728 kb on - strand at 1949.857 kb on + strand, within Pf1N1B4_1703 at 1949.857 kb on + strand, within Pf1N1B4_1703 at 1949.857 kb on + strand, within Pf1N1B4_1703 at 1949.858 kb on - strand, within Pf1N1B4_1703 at 1949.977 kb on + strand, within Pf1N1B4_1703 at 1949.978 kb on - strand, within Pf1N1B4_1703 at 1950.175 kb on - strand, within Pf1N1B4_1703 at 1950.175 kb on - strand, within Pf1N1B4_1703 at 1950.281 kb on + strand, within Pf1N1B4_1703 at 1950.281 kb on + strand, within Pf1N1B4_1703 at 1950.363 kb on + strand at 1950.379 kb on + strand at 1950.379 kb on + strand at 1950.379 kb on + strand at 1950.380 kb on - strand at 1950.402 kb on + strand at 1950.402 kb on + strand at 1950.403 kb on - strand at 1950.541 kb on + strand at 1950.542 kb on - strand at 1950.542 kb on - strand at 1950.548 kb on + strand at 1950.562 kb on + strand at 1950.562 kb on + strand at 1950.586 kb on + strand at 1950.586 kb on + strand at 1950.586 kb on + strand at 1950.587 kb on - strand at 1950.587 kb on - strand at 1950.607 kb on + strand at 1950.687 kb on + strand at 1950.688 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 1,947,635 - -0.9 1,947,783 + Pf1N1B4_1700 0.74 +1.4 1,947,944 + Pf1N1B4_1701 0.17 -0.7 1,947,944 + Pf1N1B4_1701 0.17 -1.9 1,947,945 - Pf1N1B4_1701 0.17 +0.5 1,948,045 + Pf1N1B4_1701 0.30 +0.1 1,948,045 + Pf1N1B4_1701 0.30 -0.0 1,948,046 - Pf1N1B4_1701 0.30 -1.5 1,948,153 + Pf1N1B4_1701 0.43 +1.8 1,948,153 + Pf1N1B4_1701 0.43 +0.5 1,948,363 + Pf1N1B4_1701 0.69 -0.4 1,948,399 + Pf1N1B4_1701 0.73 -0.7 1,948,399 + Pf1N1B4_1701 0.73 -1.3 1,948,400 - Pf1N1B4_1701 0.73 +0.7 1,948,400 - Pf1N1B4_1701 0.73 +0.4 1,948,469 - Pf1N1B4_1701 0.82 -2.2 1,948,469 - Pf1N1B4_1701 0.82 -0.3 1,948,469 - Pf1N1B4_1701 0.82 -0.2 1,948,537 + +1.2 1,948,537 + -0.2 1,948,537 + +0.2 1,948,538 - -0.0 1,948,538 - +2.0 1,948,538 - +0.1 1,948,538 - -0.7 1,948,538 - +0.0 1,948,538 - +0.1 1,948,538 - -0.5 1,948,592 - +0.3 1,948,661 + -0.7 1,948,661 + -0.1 1,948,734 + Pf1N1B4_1702 0.10 +0.7 1,948,735 - Pf1N1B4_1702 0.11 +0.4 1,948,735 - Pf1N1B4_1702 0.11 -0.4 1,949,727 + -0.1 1,949,727 + +0.5 1,949,727 + -1.6 1,949,727 + +1.1 1,949,728 - +0.9 1,949,728 - +0.1 1,949,857 + Pf1N1B4_1703 0.20 +1.4 1,949,857 + Pf1N1B4_1703 0.20 -1.0 1,949,857 + Pf1N1B4_1703 0.20 -0.7 1,949,858 - Pf1N1B4_1703 0.20 -1.2 1,949,977 + Pf1N1B4_1703 0.39 +0.1 1,949,978 - Pf1N1B4_1703 0.39 -0.2 1,950,175 - Pf1N1B4_1703 0.69 +1.5 1,950,175 - Pf1N1B4_1703 0.69 -2.1 1,950,281 + Pf1N1B4_1703 0.86 -1.5 1,950,281 + Pf1N1B4_1703 0.86 -0.9 1,950,363 + -0.3 1,950,379 + -2.5 1,950,379 + +0.4 1,950,379 + +1.3 1,950,380 - +1.1 1,950,402 + +0.7 1,950,402 + -0.4 1,950,403 - +1.0 1,950,541 + -1.9 1,950,542 - +0.8 1,950,542 - -0.1 1,950,548 + +0.3 1,950,562 + +0.1 1,950,562 + -0.9 1,950,586 + +0.9 1,950,586 + -0.4 1,950,586 + -0.3 1,950,587 - -0.1 1,950,587 - -0.2 1,950,607 + -0.5 1,950,687 + -0.2 1,950,688 - +1.3
Or see this region's nucleotide sequence