Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1701

Experiment: nitrogen source nitrite 0.5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1699 and Pf1N1B4_1700 overlap by 4 nucleotidesPf1N1B4_1700 and Pf1N1B4_1701 overlap by 24 nucleotidesPf1N1B4_1701 and Pf1N1B4_1702 are separated by 2 nucleotides Pf1N1B4_1699 - Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases, at 1,946,300 to 1,947,658 _1699 Pf1N1B4_1700 - hypothetical protein, at 1,947,655 to 1,947,828 _1700 Pf1N1B4_1701 - Methionine ABC transporter substrate-binding protein, at 1,947,805 to 1,948,614 _1701 Pf1N1B4_1702 - Methionine ABC transporter ATP-binding protein, at 1,948,617 to 1,949,738 _1702 Position (kb) 1947 1948 1949Strain fitness (log2 ratio) -2 -1 0 1 2at 1946.813 kb on + strand, within Pf1N1B4_1699at 1946.814 kb on - strand, within Pf1N1B4_1699at 1946.814 kb on - strand, within Pf1N1B4_1699at 1946.876 kb on + strand, within Pf1N1B4_1699at 1946.876 kb on + strand, within Pf1N1B4_1699at 1946.876 kb on + strand, within Pf1N1B4_1699at 1946.877 kb on - strand, within Pf1N1B4_1699at 1946.924 kb on + strandat 1946.925 kb on - strand, within Pf1N1B4_1699at 1946.925 kb on - strand, within Pf1N1B4_1699at 1947.203 kb on + strand, within Pf1N1B4_1699at 1947.203 kb on + strand, within Pf1N1B4_1699at 1947.212 kb on + strand, within Pf1N1B4_1699at 1947.213 kb on - strand, within Pf1N1B4_1699at 1947.213 kb on - strand, within Pf1N1B4_1699at 1947.275 kb on + strand, within Pf1N1B4_1699at 1947.299 kb on + strand, within Pf1N1B4_1699at 1947.299 kb on + strand, within Pf1N1B4_1699at 1947.300 kb on - strand, within Pf1N1B4_1699at 1947.300 kb on - strandat 1947.398 kb on + strand, within Pf1N1B4_1699at 1947.398 kb on + strand, within Pf1N1B4_1699at 1947.509 kb on + strand, within Pf1N1B4_1699at 1947.509 kb on + strand, within Pf1N1B4_1699at 1947.509 kb on + strand, within Pf1N1B4_1699at 1947.509 kb on + strand, within Pf1N1B4_1699at 1947.509 kb on + strand, within Pf1N1B4_1699at 1947.509 kb on + strand, within Pf1N1B4_1699at 1947.509 kb on + strandat 1947.510 kb on - strand, within Pf1N1B4_1699at 1947.510 kb on - strand, within Pf1N1B4_1699at 1947.510 kb on - strand, within Pf1N1B4_1699at 1947.510 kb on - strand, within Pf1N1B4_1699at 1947.560 kb on + strandat 1947.560 kb on + strandat 1947.560 kb on + strandat 1947.560 kb on + strandat 1947.572 kb on + strandat 1947.635 kb on - strandat 1947.783 kb on + strand, within Pf1N1B4_1700at 1947.944 kb on + strand, within Pf1N1B4_1701at 1947.944 kb on + strand, within Pf1N1B4_1701at 1947.945 kb on - strand, within Pf1N1B4_1701at 1948.045 kb on + strand, within Pf1N1B4_1701at 1948.045 kb on + strand, within Pf1N1B4_1701at 1948.046 kb on - strand, within Pf1N1B4_1701at 1948.153 kb on + strand, within Pf1N1B4_1701at 1948.153 kb on + strand, within Pf1N1B4_1701at 1948.363 kb on + strand, within Pf1N1B4_1701at 1948.399 kb on + strand, within Pf1N1B4_1701at 1948.399 kb on + strand, within Pf1N1B4_1701at 1948.400 kb on - strand, within Pf1N1B4_1701at 1948.400 kb on - strand, within Pf1N1B4_1701at 1948.469 kb on - strand, within Pf1N1B4_1701at 1948.469 kb on - strand, within Pf1N1B4_1701at 1948.469 kb on - strand, within Pf1N1B4_1701at 1948.537 kb on + strandat 1948.537 kb on + strandat 1948.537 kb on + strandat 1948.538 kb on - strandat 1948.538 kb on - strandat 1948.538 kb on - strandat 1948.538 kb on - strandat 1948.538 kb on - strandat 1948.538 kb on - strandat 1948.538 kb on - strandat 1948.592 kb on - strandat 1948.661 kb on + strandat 1948.661 kb on + strandat 1948.734 kb on + strand, within Pf1N1B4_1702at 1948.735 kb on - strand, within Pf1N1B4_1702at 1948.735 kb on - strand, within Pf1N1B4_1702

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM
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1,946,813 + Pf1N1B4_1699 0.38 -0.5
1,946,814 - Pf1N1B4_1699 0.38 +1.4
1,946,814 - Pf1N1B4_1699 0.38 +0.0
1,946,876 + Pf1N1B4_1699 0.42 -0.2
1,946,876 + Pf1N1B4_1699 0.42 +0.9
1,946,876 + Pf1N1B4_1699 0.42 -0.4
1,946,877 - Pf1N1B4_1699 0.42 +0.1
1,946,924 + +2.1
1,946,925 - Pf1N1B4_1699 0.46 -2.4
1,946,925 - Pf1N1B4_1699 0.46 +1.2
1,947,203 + Pf1N1B4_1699 0.66 -0.1
1,947,203 + Pf1N1B4_1699 0.66 -0.6
1,947,212 + Pf1N1B4_1699 0.67 -0.3
1,947,213 - Pf1N1B4_1699 0.67 -0.4
1,947,213 - Pf1N1B4_1699 0.67 +0.0
1,947,275 + Pf1N1B4_1699 0.72 +1.3
1,947,299 + Pf1N1B4_1699 0.74 +1.1
1,947,299 + Pf1N1B4_1699 0.74 -2.2
1,947,300 - Pf1N1B4_1699 0.74 -2.3
1,947,300 - +0.0
1,947,398 + Pf1N1B4_1699 0.81 -1.5
1,947,398 + Pf1N1B4_1699 0.81 -0.1
1,947,509 + Pf1N1B4_1699 0.89 -1.3
1,947,509 + Pf1N1B4_1699 0.89 -1.6
1,947,509 + Pf1N1B4_1699 0.89 -2.6
1,947,509 + Pf1N1B4_1699 0.89 -1.9
1,947,509 + Pf1N1B4_1699 0.89 -0.1
1,947,509 + Pf1N1B4_1699 0.89 +0.4
1,947,509 + +1.7
1,947,510 - Pf1N1B4_1699 0.89 -0.7
1,947,510 - Pf1N1B4_1699 0.89 +2.1
1,947,510 - Pf1N1B4_1699 0.89 +1.3
1,947,510 - Pf1N1B4_1699 0.89 +1.5
1,947,560 + -1.0
1,947,560 + +0.8
1,947,560 + +0.6
1,947,560 + -0.5
1,947,572 + +0.4
1,947,635 - -0.9
1,947,783 + Pf1N1B4_1700 0.74 +1.4
1,947,944 + Pf1N1B4_1701 0.17 -0.7
1,947,944 + Pf1N1B4_1701 0.17 -1.9
1,947,945 - Pf1N1B4_1701 0.17 +0.5
1,948,045 + Pf1N1B4_1701 0.30 +0.1
1,948,045 + Pf1N1B4_1701 0.30 -0.0
1,948,046 - Pf1N1B4_1701 0.30 -1.5
1,948,153 + Pf1N1B4_1701 0.43 +1.8
1,948,153 + Pf1N1B4_1701 0.43 +0.5
1,948,363 + Pf1N1B4_1701 0.69 -0.4
1,948,399 + Pf1N1B4_1701 0.73 -0.7
1,948,399 + Pf1N1B4_1701 0.73 -1.3
1,948,400 - Pf1N1B4_1701 0.73 +0.7
1,948,400 - Pf1N1B4_1701 0.73 +0.4
1,948,469 - Pf1N1B4_1701 0.82 -2.2
1,948,469 - Pf1N1B4_1701 0.82 -0.3
1,948,469 - Pf1N1B4_1701 0.82 -0.2
1,948,537 + +1.2
1,948,537 + -0.2
1,948,537 + +0.2
1,948,538 - -0.0
1,948,538 - +2.0
1,948,538 - +0.1
1,948,538 - -0.7
1,948,538 - +0.0
1,948,538 - +0.1
1,948,538 - -0.5
1,948,592 - +0.3
1,948,661 + -0.7
1,948,661 + -0.1
1,948,734 + Pf1N1B4_1702 0.10 +0.7
1,948,735 - Pf1N1B4_1702 0.11 +0.4
1,948,735 - Pf1N1B4_1702 0.11 -0.4

Or see this region's nucleotide sequence