Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1143 and Pf1N1B4_1144 are separated by 167 nucleotides Pf1N1B4_1144 and Pf1N1B4_1145 are separated by 11 nucleotides Pf1N1B4_1145 and Pf1N1B4_1146 overlap by 1 nucleotides
Pf1N1B4_1143 - Phosphodiesterase/alkaline phosphatase D, at 1,285,072 to 1,286,613
_1143
Pf1N1B4_1144 - PTS system, fructose-specific IIB component (EC 2.7.1.69) / PTS system, fructose-specific IIC component (EC 2.7.1.69), at 1,286,781 to 1,288,520
_1144
Pf1N1B4_1145 - 1-phosphofructokinase (EC 2.7.1.56), at 1,288,532 to 1,289,473
_1145
Pf1N1B4_1146 - fructose-specific PTS system (fructose 1-phosphate forming), EI, Hpr, and EII-A components (from data) , at 1,289,473 to 1,292,334
_1146
Position (kb)
1286
1287
1288
1289 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1285.815 kb on + strand, within Pf1N1B4_1143 at 1285.829 kb on + strand, within Pf1N1B4_1143 at 1285.946 kb on + strand, within Pf1N1B4_1143 at 1285.953 kb on - strand, within Pf1N1B4_1143 at 1285.965 kb on - strand, within Pf1N1B4_1143 at 1286.070 kb on - strand, within Pf1N1B4_1143 at 1286.126 kb on + strand, within Pf1N1B4_1143 at 1286.126 kb on + strand, within Pf1N1B4_1143 at 1286.126 kb on + strand, within Pf1N1B4_1143 at 1286.127 kb on - strand, within Pf1N1B4_1143 at 1286.127 kb on - strand, within Pf1N1B4_1143 at 1286.203 kb on - strand, within Pf1N1B4_1143 at 1286.388 kb on + strand at 1286.640 kb on + strand at 1286.640 kb on + strand at 1286.641 kb on - strand at 1286.641 kb on - strand at 1286.641 kb on - strand at 1286.641 kb on - strand at 1286.641 kb on - strand at 1286.738 kb on + strand at 1286.776 kb on + strand at 1286.777 kb on - strand at 1286.839 kb on + strand at 1286.839 kb on + strand at 1286.840 kb on - strand at 1286.840 kb on - strand at 1286.840 kb on - strand at 1286.840 kb on - strand at 1286.884 kb on + strand at 1286.884 kb on + strand at 1286.884 kb on + strand at 1286.885 kb on - strand at 1286.965 kb on + strand, within Pf1N1B4_1144 at 1286.965 kb on + strand, within Pf1N1B4_1144 at 1286.966 kb on - strand, within Pf1N1B4_1144 at 1286.966 kb on - strand, within Pf1N1B4_1144 at 1286.966 kb on - strand, within Pf1N1B4_1144 at 1286.966 kb on - strand, within Pf1N1B4_1144 at 1286.966 kb on - strand, within Pf1N1B4_1144 at 1286.966 kb on - strand, within Pf1N1B4_1144 at 1287.063 kb on - strand, within Pf1N1B4_1144 at 1287.063 kb on - strand, within Pf1N1B4_1144 at 1287.063 kb on - strand, within Pf1N1B4_1144 at 1287.203 kb on - strand, within Pf1N1B4_1144 at 1287.385 kb on + strand, within Pf1N1B4_1144 at 1287.385 kb on + strand, within Pf1N1B4_1144 at 1287.385 kb on + strand, within Pf1N1B4_1144 at 1287.385 kb on + strand, within Pf1N1B4_1144 at 1287.385 kb on + strand, within Pf1N1B4_1144 at 1287.385 kb on + strand, within Pf1N1B4_1144 at 1287.385 kb on + strand, within Pf1N1B4_1144 at 1287.385 kb on + strand, within Pf1N1B4_1144 at 1287.385 kb on + strand, within Pf1N1B4_1144 at 1287.385 kb on + strand, within Pf1N1B4_1144 at 1287.385 kb on + strand at 1287.386 kb on - strand, within Pf1N1B4_1144 at 1287.386 kb on - strand, within Pf1N1B4_1144 at 1287.386 kb on - strand, within Pf1N1B4_1144 at 1287.386 kb on - strand, within Pf1N1B4_1144 at 1287.386 kb on - strand, within Pf1N1B4_1144 at 1287.386 kb on - strand, within Pf1N1B4_1144 at 1287.386 kb on - strand, within Pf1N1B4_1144 at 1287.386 kb on - strand, within Pf1N1B4_1144 at 1287.386 kb on - strand, within Pf1N1B4_1144 at 1287.386 kb on - strand, within Pf1N1B4_1144 at 1287.386 kb on - strand, within Pf1N1B4_1144 at 1287.386 kb on - strand, within Pf1N1B4_1144 at 1287.386 kb on - strand, within Pf1N1B4_1144 at 1287.386 kb on - strand, within Pf1N1B4_1144 at 1287.386 kb on - strand, within Pf1N1B4_1144 at 1287.386 kb on - strand, within Pf1N1B4_1144 at 1287.386 kb on - strand, within Pf1N1B4_1144 at 1287.386 kb on - strand, within Pf1N1B4_1144 at 1287.386 kb on - strand, within Pf1N1B4_1144 at 1287.475 kb on + strand at 1287.619 kb on + strand, within Pf1N1B4_1144 at 1287.619 kb on + strand, within Pf1N1B4_1144 at 1287.620 kb on - strand, within Pf1N1B4_1144 at 1287.620 kb on - strand, within Pf1N1B4_1144 at 1287.620 kb on - strand at 1287.802 kb on + strand, within Pf1N1B4_1144 at 1287.802 kb on + strand, within Pf1N1B4_1144 at 1287.802 kb on + strand, within Pf1N1B4_1144 at 1287.802 kb on + strand at 1287.802 kb on + strand, within Pf1N1B4_1144 at 1287.802 kb on + strand, within Pf1N1B4_1144 at 1287.802 kb on + strand, within Pf1N1B4_1144 at 1287.802 kb on + strand, within Pf1N1B4_1144 at 1287.803 kb on - strand, within Pf1N1B4_1144 at 1287.803 kb on - strand, within Pf1N1B4_1144 at 1287.803 kb on - strand, within Pf1N1B4_1144 at 1287.803 kb on - strand, within Pf1N1B4_1144 at 1287.803 kb on - strand, within Pf1N1B4_1144 at 1288.213 kb on + strand, within Pf1N1B4_1144 at 1288.214 kb on - strand, within Pf1N1B4_1144 at 1288.214 kb on - strand, within Pf1N1B4_1144 at 1288.214 kb on - strand, within Pf1N1B4_1144 at 1288.316 kb on + strand, within Pf1N1B4_1144 at 1288.316 kb on + strand, within Pf1N1B4_1144 at 1288.316 kb on + strand, within Pf1N1B4_1144 at 1288.316 kb on + strand, within Pf1N1B4_1144 at 1288.317 kb on - strand, within Pf1N1B4_1144 at 1288.513 kb on - strand at 1288.533 kb on + strand at 1288.533 kb on + strand at 1288.533 kb on + strand at 1288.533 kb on + strand at 1288.534 kb on - strand at 1288.534 kb on - strand at 1288.534 kb on - strand at 1288.534 kb on - strand at 1288.534 kb on - strand at 1288.534 kb on - strand at 1289.197 kb on + strand, within Pf1N1B4_1145 at 1289.489 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 1,285,815 + Pf1N1B4_1143 0.48 +0.6 1,285,829 + Pf1N1B4_1143 0.49 -0.3 1,285,946 + Pf1N1B4_1143 0.57 -0.7 1,285,953 - Pf1N1B4_1143 0.57 -0.2 1,285,965 - Pf1N1B4_1143 0.58 -0.0 1,286,070 - Pf1N1B4_1143 0.65 -0.0 1,286,126 + Pf1N1B4_1143 0.68 +0.6 1,286,126 + Pf1N1B4_1143 0.68 +0.9 1,286,126 + Pf1N1B4_1143 0.68 +2.0 1,286,127 - Pf1N1B4_1143 0.68 +1.0 1,286,127 - Pf1N1B4_1143 0.68 -1.4 1,286,203 - Pf1N1B4_1143 0.73 -0.0 1,286,388 + +2.1 1,286,640 + +0.8 1,286,640 + +1.8 1,286,641 - +3.1 1,286,641 - +0.5 1,286,641 - +0.0 1,286,641 - -0.9 1,286,641 - -1.8 1,286,738 + +0.8 1,286,776 + +0.2 1,286,777 - -0.1 1,286,839 + -1.5 1,286,839 + -0.2 1,286,840 - +0.1 1,286,840 - -0.8 1,286,840 - +0.9 1,286,840 - -1.2 1,286,884 + +0.3 1,286,884 + +1.0 1,286,884 + -1.1 1,286,885 - -0.8 1,286,965 + Pf1N1B4_1144 0.11 -0.4 1,286,965 + Pf1N1B4_1144 0.11 -1.1 1,286,966 - Pf1N1B4_1144 0.11 +0.7 1,286,966 - Pf1N1B4_1144 0.11 -1.3 1,286,966 - Pf1N1B4_1144 0.11 -0.4 1,286,966 - Pf1N1B4_1144 0.11 +0.8 1,286,966 - Pf1N1B4_1144 0.11 -0.2 1,286,966 - Pf1N1B4_1144 0.11 +1.1 1,287,063 - Pf1N1B4_1144 0.16 -1.9 1,287,063 - Pf1N1B4_1144 0.16 -0.7 1,287,063 - Pf1N1B4_1144 0.16 -0.9 1,287,203 - Pf1N1B4_1144 0.24 -1.5 1,287,385 + Pf1N1B4_1144 0.35 +0.3 1,287,385 + Pf1N1B4_1144 0.35 +1.4 1,287,385 + Pf1N1B4_1144 0.35 -1.5 1,287,385 + Pf1N1B4_1144 0.35 -2.0 1,287,385 + Pf1N1B4_1144 0.35 -0.5 1,287,385 + Pf1N1B4_1144 0.35 -2.9 1,287,385 + Pf1N1B4_1144 0.35 -1.1 1,287,385 + Pf1N1B4_1144 0.35 -0.0 1,287,385 + Pf1N1B4_1144 0.35 -0.7 1,287,385 + Pf1N1B4_1144 0.35 +0.3 1,287,385 + +0.5 1,287,386 - Pf1N1B4_1144 0.35 -0.3 1,287,386 - Pf1N1B4_1144 0.35 -2.4 1,287,386 - Pf1N1B4_1144 0.35 -1.2 1,287,386 - Pf1N1B4_1144 0.35 -0.6 1,287,386 - Pf1N1B4_1144 0.35 -0.9 1,287,386 - Pf1N1B4_1144 0.35 +0.4 1,287,386 - Pf1N1B4_1144 0.35 +1.1 1,287,386 - Pf1N1B4_1144 0.35 +0.7 1,287,386 - Pf1N1B4_1144 0.35 -0.7 1,287,386 - Pf1N1B4_1144 0.35 -1.6 1,287,386 - Pf1N1B4_1144 0.35 -0.5 1,287,386 - Pf1N1B4_1144 0.35 -0.1 1,287,386 - Pf1N1B4_1144 0.35 -1.2 1,287,386 - Pf1N1B4_1144 0.35 -1.4 1,287,386 - Pf1N1B4_1144 0.35 -1.7 1,287,386 - Pf1N1B4_1144 0.35 +0.9 1,287,386 - Pf1N1B4_1144 0.35 -0.7 1,287,386 - Pf1N1B4_1144 0.35 -2.9 1,287,386 - Pf1N1B4_1144 0.35 -1.2 1,287,475 + +0.6 1,287,619 + Pf1N1B4_1144 0.48 -1.9 1,287,619 + Pf1N1B4_1144 0.48 -1.5 1,287,620 - Pf1N1B4_1144 0.48 -1.6 1,287,620 - Pf1N1B4_1144 0.48 -0.5 1,287,620 - +1.7 1,287,802 + Pf1N1B4_1144 0.59 -1.5 1,287,802 + Pf1N1B4_1144 0.59 -0.0 1,287,802 + Pf1N1B4_1144 0.59 -0.7 1,287,802 + -1.8 1,287,802 + Pf1N1B4_1144 0.59 +1.6 1,287,802 + Pf1N1B4_1144 0.59 -1.1 1,287,802 + Pf1N1B4_1144 0.59 +0.3 1,287,802 + Pf1N1B4_1144 0.59 +0.5 1,287,803 - Pf1N1B4_1144 0.59 +0.6 1,287,803 - Pf1N1B4_1144 0.59 -2.0 1,287,803 - Pf1N1B4_1144 0.59 -1.6 1,287,803 - Pf1N1B4_1144 0.59 -1.9 1,287,803 - Pf1N1B4_1144 0.59 +1.4 1,288,213 + Pf1N1B4_1144 0.82 -2.3 1,288,214 - Pf1N1B4_1144 0.82 +0.1 1,288,214 - Pf1N1B4_1144 0.82 -1.5 1,288,214 - Pf1N1B4_1144 0.82 -1.1 1,288,316 + Pf1N1B4_1144 0.88 +1.8 1,288,316 + Pf1N1B4_1144 0.88 -1.1 1,288,316 + Pf1N1B4_1144 0.88 -1.6 1,288,316 + Pf1N1B4_1144 0.88 -0.5 1,288,317 - Pf1N1B4_1144 0.88 -0.9 1,288,513 - +0.2 1,288,533 + +0.2 1,288,533 + -1.5 1,288,533 + +1.0 1,288,533 + -0.1 1,288,534 - +1.0 1,288,534 - +1.9 1,288,534 - +0.9 1,288,534 - +0.1 1,288,534 - -1.3 1,288,534 - +0.1 1,289,197 + Pf1N1B4_1145 0.71 +2.7 1,289,489 - -0.9
Or see this region's nucleotide sequence