Experiment: nitrogen source nitrite 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5892 and Pf1N1B4_5893 overlap by 4 nucleotides Pf1N1B4_5893 and Pf1N1B4_5894 overlap by 8 nucleotides Pf1N1B4_5894 and Pf1N1B4_5895 are separated by 12 nucleotides
Pf1N1B4_5892 - FIG00961753: hypothetical protein, at 336,034 to 336,216
_5892
Pf1N1B4_5893 - Mycofactocin system glycosyltransferase, at 336,213 to 337,190
_5893
Pf1N1B4_5894 - Glycosyltransferase, at 337,183 to 338,319
_5894
Pf1N1B4_5895 - FIG00958408: hypothetical protein, at 338,332 to 339,333
_5895
Position (kb)
337
338
339 Strain fitness (log2 ratio)
-2
-1
0
1 at 336.252 kb on + strand at 336.252 kb on + strand at 336.279 kb on - strand at 337.015 kb on + strand, within Pf1N1B4_5893 at 337.016 kb on - strand, within Pf1N1B4_5893 at 337.078 kb on + strand, within Pf1N1B4_5893 at 337.079 kb on - strand at 337.079 kb on - strand, within Pf1N1B4_5893 at 337.225 kb on + strand at 337.225 kb on + strand at 337.225 kb on + strand at 337.226 kb on - strand at 337.226 kb on - strand at 337.364 kb on + strand, within Pf1N1B4_5894 at 337.712 kb on - strand, within Pf1N1B4_5894 at 337.876 kb on + strand, within Pf1N1B4_5894 at 337.877 kb on - strand, within Pf1N1B4_5894 at 338.018 kb on - strand, within Pf1N1B4_5894 at 338.018 kb on - strand at 338.979 kb on - strand, within Pf1N1B4_5895
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 1mM remove 336,252 + +0.5 336,252 + -2.6 336,279 - +0.0 337,015 + Pf1N1B4_5893 0.82 -0.5 337,016 - Pf1N1B4_5893 0.82 +0.4 337,078 + Pf1N1B4_5893 0.88 -0.3 337,079 - -0.8 337,079 - Pf1N1B4_5893 0.89 -1.6 337,225 + -0.1 337,225 + +0.1 337,225 + +0.2 337,226 - -0.7 337,226 - +0.0 337,364 + Pf1N1B4_5894 0.16 -1.5 337,712 - Pf1N1B4_5894 0.47 -1.2 337,876 + Pf1N1B4_5894 0.61 -0.7 337,877 - Pf1N1B4_5894 0.61 -0.6 338,018 - Pf1N1B4_5894 0.73 +0.7 338,018 - +0.1 338,979 - Pf1N1B4_5895 0.65 -1.8
Or see this region's nucleotide sequence