Experiment: nitrogen source nitrite 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4502 and Pf1N1B4_4503 are separated by 385 nucleotides Pf1N1B4_4503 and Pf1N1B4_4504 are separated by 3 nucleotides Pf1N1B4_4504 and Pf1N1B4_4505 overlap by 23 nucleotides
Pf1N1B4_4502 - Aldehyde dehydrogenase (EC 1.2.1.3), at 4,963,641 to 4,965,161
_4502
Pf1N1B4_4503 - Coenzyme PQQ synthesis protein B, at 4,965,547 to 4,966,461
_4503
Pf1N1B4_4504 - Pyrroloquinoline-quinone synthase (EC 1.3.3.11), at 4,966,465 to 4,966,872
_4504
Pf1N1B4_4505 - Coenzyme PQQ synthesis protein E, at 4,966,850 to 4,968,487
_4505
Position (kb)
4965
4966
4967 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4964.822 kb on + strand, within Pf1N1B4_4502 at 4964.823 kb on - strand, within Pf1N1B4_4502 at 4965.232 kb on + strand at 4965.233 kb on - strand at 4965.290 kb on + strand at 4965.347 kb on - strand at 4965.475 kb on + strand at 4965.475 kb on + strand at 4965.476 kb on - strand at 4965.476 kb on - strand at 4966.359 kb on + strand, within Pf1N1B4_4503 at 4966.436 kb on - strand at 4966.449 kb on + strand at 4966.522 kb on + strand at 4966.700 kb on - strand, within Pf1N1B4_4504 at 4966.754 kb on - strand, within Pf1N1B4_4504 at 4967.118 kb on - strand, within Pf1N1B4_4505 at 4967.124 kb on - strand, within Pf1N1B4_4505 at 4967.192 kb on + strand, within Pf1N1B4_4505 at 4967.435 kb on + strand, within Pf1N1B4_4505
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 1mM remove 4,964,822 + Pf1N1B4_4502 0.78 -0.7 4,964,823 - Pf1N1B4_4502 0.78 -1.1 4,965,232 + -2.6 4,965,233 - -0.3 4,965,290 + +0.4 4,965,347 - -0.5 4,965,475 + -0.4 4,965,475 + -2.5 4,965,476 - +1.6 4,965,476 - +0.2 4,966,359 + Pf1N1B4_4503 0.89 +0.1 4,966,436 - +0.5 4,966,449 + -1.2 4,966,522 + -1.4 4,966,700 - Pf1N1B4_4504 0.58 +0.9 4,966,754 - Pf1N1B4_4504 0.71 -1.9 4,967,118 - Pf1N1B4_4505 0.16 -1.1 4,967,124 - Pf1N1B4_4505 0.17 +2.3 4,967,192 + Pf1N1B4_4505 0.21 -0.2 4,967,435 + Pf1N1B4_4505 0.36 -0.1
Or see this region's nucleotide sequence