Experiment: nitrogen source nitrate 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5756 and Pf1N1B4_5757 overlap by 4 nucleotides Pf1N1B4_5757 and Pf1N1B4_5758 are separated by 195 nucleotides
Pf1N1B4_5756 - Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family), at 196,193 to 196,876
_5756
Pf1N1B4_5757 - hypothetical protein, at 196,873 to 197,067
_5757
Pf1N1B4_5758 - Nitric oxide reductase activation protein NorD, at 197,263 to 199,104
_5758
Position (kb) 196
197
198 Strain fitness (log2 ratio) -2
-1
0
1
2 at 195.926 kb on + strand at 195.927 kb on - strand at 196.142 kb on + strand at 196.352 kb on + strand, within Pf1N1B4_5756 at 196.353 kb on - strand, within Pf1N1B4_5756 at 196.479 kb on - strand, within Pf1N1B4_5756 at 196.538 kb on + strand, within Pf1N1B4_5756 at 196.539 kb on - strand, within Pf1N1B4_5756 at 196.689 kb on + strand, within Pf1N1B4_5756 at 196.897 kb on + strand, within Pf1N1B4_5757 at 196.897 kb on + strand, within Pf1N1B4_5757 at 197.078 kb on + strand at 197.078 kb on + strand at 197.079 kb on - strand at 197.079 kb on - strand at 197.237 kb on + strand at 197.308 kb on + strand at 197.309 kb on - strand at 197.357 kb on + strand at 197.357 kb on + strand at 197.358 kb on - strand at 197.366 kb on + strand at 197.394 kb on + strand at 197.471 kb on + strand, within Pf1N1B4_5758 at 197.471 kb on + strand, within Pf1N1B4_5758 at 197.472 kb on - strand, within Pf1N1B4_5758 at 197.546 kb on - strand, within Pf1N1B4_5758 at 197.546 kb on - strand, within Pf1N1B4_5758 at 197.585 kb on + strand, within Pf1N1B4_5758 at 197.586 kb on - strand, within Pf1N1B4_5758 at 197.586 kb on - strand, within Pf1N1B4_5758 at 197.586 kb on - strand, within Pf1N1B4_5758 at 197.589 kb on - strand, within Pf1N1B4_5758 at 197.764 kb on + strand, within Pf1N1B4_5758 at 197.764 kb on + strand, within Pf1N1B4_5758 at 197.764 kb on + strand, within Pf1N1B4_5758 at 197.765 kb on - strand, within Pf1N1B4_5758
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 5mM remove 195,926 + -0.1 195,927 - +2.5 196,142 + -0.3 196,352 + Pf1N1B4_5756 0.23 -0.7 196,353 - Pf1N1B4_5756 0.23 -1.7 196,479 - Pf1N1B4_5756 0.42 +0.1 196,538 + Pf1N1B4_5756 0.50 -0.4 196,539 - Pf1N1B4_5756 0.51 -0.9 196,689 + Pf1N1B4_5756 0.73 -2.0 196,897 + Pf1N1B4_5757 0.12 -0.7 196,897 + Pf1N1B4_5757 0.12 +0.9 197,078 + +0.0 197,078 + -0.1 197,079 - -0.1 197,079 - -0.2 197,237 + -1.5 197,308 + +0.8 197,309 - -0.3 197,357 + -1.1 197,357 + +0.2 197,358 - +0.3 197,366 + -1.5 197,394 + -0.7 197,471 + Pf1N1B4_5758 0.11 -2.3 197,471 + Pf1N1B4_5758 0.11 +1.0 197,472 - Pf1N1B4_5758 0.11 +0.5 197,546 - Pf1N1B4_5758 0.15 +0.3 197,546 - Pf1N1B4_5758 0.15 -0.7 197,585 + Pf1N1B4_5758 0.17 -1.6 197,586 - Pf1N1B4_5758 0.18 +0.4 197,586 - Pf1N1B4_5758 0.18 +1.3 197,586 - Pf1N1B4_5758 0.18 +0.3 197,589 - Pf1N1B4_5758 0.18 -0.1 197,764 + Pf1N1B4_5758 0.27 -0.7 197,764 + Pf1N1B4_5758 0.27 -0.1 197,764 + Pf1N1B4_5758 0.27 -1.8 197,765 - Pf1N1B4_5758 0.27 -1.8
Or see this region's nucleotide sequence