Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5735

Experiment: nitrogen source nitrate 5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5734 and Pf1N1B4_5735 are separated by 1 nucleotidesPf1N1B4_5735 and Pf1N1B4_5736 are separated by 68 nucleotides Pf1N1B4_5734 - Respiratory nitrate reductase beta chain (EC 1.7.99.4), at 173,287 to 174,825 _5734 Pf1N1B4_5735 - Respiratory nitrate reductase alpha chain (EC 1.7.99.4), at 174,827 to 178,600 _5735 Pf1N1B4_5736 - Nitrate/nitrite transporter, at 178,669 to 180,066 _5736 Position (kb) 174 175 176 177 178 179Strain fitness (log2 ratio) -2 -1 0 1at 173.838 kb on - strand, within Pf1N1B4_5734at 174.132 kb on - strand, within Pf1N1B4_5734at 174.224 kb on + strand, within Pf1N1B4_5734at 174.293 kb on + strand, within Pf1N1B4_5734at 174.293 kb on + strandat 174.293 kb on + strand, within Pf1N1B4_5734at 174.294 kb on - strand, within Pf1N1B4_5734at 174.294 kb on - strand, within Pf1N1B4_5734at 174.294 kb on - strand, within Pf1N1B4_5734at 174.525 kb on - strand, within Pf1N1B4_5734at 174.528 kb on - strand, within Pf1N1B4_5734at 174.714 kb on - strandat 174.955 kb on - strandat 174.957 kb on + strandat 174.958 kb on - strandat 174.958 kb on - strandat 174.958 kb on - strandat 175.101 kb on + strandat 175.102 kb on - strandat 175.284 kb on + strand, within Pf1N1B4_5735at 175.285 kb on - strand, within Pf1N1B4_5735at 175.522 kb on - strand, within Pf1N1B4_5735at 175.902 kb on + strandat 176.412 kb on + strand, within Pf1N1B4_5735at 176.412 kb on + strand, within Pf1N1B4_5735at 176.412 kb on + strand, within Pf1N1B4_5735at 176.544 kb on + strand, within Pf1N1B4_5735at 176.544 kb on + strand, within Pf1N1B4_5735at 176.545 kb on - strand, within Pf1N1B4_5735at 176.655 kb on + strand, within Pf1N1B4_5735at 176.655 kb on + strand, within Pf1N1B4_5735at 176.655 kb on + strand, within Pf1N1B4_5735at 176.655 kb on + strand, within Pf1N1B4_5735at 176.754 kb on + strand, within Pf1N1B4_5735at 176.859 kb on + strand, within Pf1N1B4_5735at 177.105 kb on + strand, within Pf1N1B4_5735at 177.294 kb on + strand, within Pf1N1B4_5735at 177.615 kb on + strand, within Pf1N1B4_5735at 177.615 kb on + strand, within Pf1N1B4_5735at 177.627 kb on + strand, within Pf1N1B4_5735at 177.750 kb on + strand, within Pf1N1B4_5735at 177.858 kb on + strandat 177.858 kb on + strand, within Pf1N1B4_5735at 177.858 kb on + strand, within Pf1N1B4_5735at 177.858 kb on + strand, within Pf1N1B4_5735at 177.859 kb on - strand, within Pf1N1B4_5735at 177.871 kb on - strand, within Pf1N1B4_5735at 177.951 kb on + strand, within Pf1N1B4_5735at 177.952 kb on - strand, within Pf1N1B4_5735at 177.952 kb on - strand, within Pf1N1B4_5735at 177.988 kb on - strand, within Pf1N1B4_5735at 178.063 kb on - strand, within Pf1N1B4_5735at 178.075 kb on + strand, within Pf1N1B4_5735at 178.076 kb on - strand, within Pf1N1B4_5735at 178.077 kb on + strand, within Pf1N1B4_5735at 178.077 kb on + strand, within Pf1N1B4_5735at 178.078 kb on - strand, within Pf1N1B4_5735at 178.078 kb on - strand, within Pf1N1B4_5735at 178.078 kb on - strand, within Pf1N1B4_5735at 178.156 kb on + strand, within Pf1N1B4_5735at 178.159 kb on - strand, within Pf1N1B4_5735at 178.159 kb on - strand, within Pf1N1B4_5735at 178.290 kb on + strandat 178.291 kb on - strandat 178.296 kb on + strandat 178.296 kb on + strandat 178.414 kb on - strandat 178.589 kb on + strandat 178.590 kb on - strandat 178.733 kb on + strandat 178.751 kb on + strandat 178.752 kb on - strandat 178.752 kb on - strandat 178.752 kb on - strandat 178.752 kb on - strandat 178.752 kb on - strandat 178.752 kb on - strandat 178.752 kb on - strandat 178.752 kb on - strandat 179.406 kb on + strand, within Pf1N1B4_5736at 179.406 kb on + strand, within Pf1N1B4_5736at 179.407 kb on - strand, within Pf1N1B4_5736at 179.407 kb on - strand, within Pf1N1B4_5736at 179.407 kb on - strandat 179.505 kb on + strand, within Pf1N1B4_5736

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrate 5mM
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173,838 - Pf1N1B4_5734 0.36 -1.8
174,132 - Pf1N1B4_5734 0.55 -0.3
174,224 + Pf1N1B4_5734 0.61 -0.4
174,293 + Pf1N1B4_5734 0.65 -2.2
174,293 + -2.4
174,293 + Pf1N1B4_5734 0.65 -0.3
174,294 - Pf1N1B4_5734 0.65 +0.1
174,294 - Pf1N1B4_5734 0.65 -0.8
174,294 - Pf1N1B4_5734 0.65 -0.4
174,525 - Pf1N1B4_5734 0.80 -0.8
174,528 - Pf1N1B4_5734 0.81 +0.9
174,714 - +1.3
174,955 - +0.4
174,957 + -0.8
174,958 - -0.4
174,958 - -0.5
174,958 - +0.6
175,101 + -0.2
175,102 - -1.2
175,284 + Pf1N1B4_5735 0.12 +1.4
175,285 - Pf1N1B4_5735 0.12 -1.4
175,522 - Pf1N1B4_5735 0.18 -1.6
175,902 + +0.1
176,412 + Pf1N1B4_5735 0.42 +0.7
176,412 + Pf1N1B4_5735 0.42 +0.4
176,412 + Pf1N1B4_5735 0.42 +0.5
176,544 + Pf1N1B4_5735 0.45 +0.2
176,544 + Pf1N1B4_5735 0.45 +0.4
176,545 - Pf1N1B4_5735 0.46 +0.1
176,655 + Pf1N1B4_5735 0.48 -0.6
176,655 + Pf1N1B4_5735 0.48 +0.5
176,655 + Pf1N1B4_5735 0.48 +1.4
176,655 + Pf1N1B4_5735 0.48 -1.2
176,754 + Pf1N1B4_5735 0.51 +0.0
176,859 + Pf1N1B4_5735 0.54 -0.9
177,105 + Pf1N1B4_5735 0.60 -0.2
177,294 + Pf1N1B4_5735 0.65 +0.7
177,615 + Pf1N1B4_5735 0.74 +1.5
177,615 + Pf1N1B4_5735 0.74 -1.3
177,627 + Pf1N1B4_5735 0.74 -0.2
177,750 + Pf1N1B4_5735 0.77 -0.9
177,858 + +0.3
177,858 + Pf1N1B4_5735 0.80 -0.8
177,858 + Pf1N1B4_5735 0.80 +0.1
177,858 + Pf1N1B4_5735 0.80 +0.1
177,859 - Pf1N1B4_5735 0.80 -0.5
177,871 - Pf1N1B4_5735 0.81 +1.1
177,951 + Pf1N1B4_5735 0.83 -0.8
177,952 - Pf1N1B4_5735 0.83 +0.6
177,952 - Pf1N1B4_5735 0.83 -0.5
177,988 - Pf1N1B4_5735 0.84 -0.1
178,063 - Pf1N1B4_5735 0.86 -0.6
178,075 + Pf1N1B4_5735 0.86 +0.7
178,076 - Pf1N1B4_5735 0.86 -0.5
178,077 + Pf1N1B4_5735 0.86 +0.6
178,077 + Pf1N1B4_5735 0.86 +1.5
178,078 - Pf1N1B4_5735 0.86 -0.6
178,078 - Pf1N1B4_5735 0.86 +0.4
178,078 - Pf1N1B4_5735 0.86 -1.4
178,156 + Pf1N1B4_5735 0.88 +0.5
178,159 - Pf1N1B4_5735 0.88 -0.3
178,159 - Pf1N1B4_5735 0.88 +1.4
178,290 + +0.3
178,291 - +0.2
178,296 + -0.1
178,296 + +0.5
178,414 - +0.3
178,589 + -0.4
178,590 - -0.1
178,733 + -0.6
178,751 + -0.4
178,752 - -1.5
178,752 - -1.3
178,752 - -2.1
178,752 - -0.2
178,752 - +0.2
178,752 - -1.5
178,752 - -0.6
178,752 - +1.4
179,406 + Pf1N1B4_5736 0.53 +0.4
179,406 + Pf1N1B4_5736 0.53 -0.2
179,407 - Pf1N1B4_5736 0.53 +0.8
179,407 - Pf1N1B4_5736 0.53 -1.3
179,407 - -2.3
179,505 + Pf1N1B4_5736 0.60 -0.1

Or see this region's nucleotide sequence