Experiment: nitrogen source nitrate 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5676 and Pf1N1B4_5677 are separated by 218 nucleotides Pf1N1B4_5677 and Pf1N1B4_5678 are separated by 157 nucleotides Pf1N1B4_5678 and Pf1N1B4_5679 are separated by 179 nucleotides
Pf1N1B4_5676 - hypothetical protein, at 113,412 to 113,654
_5676
Pf1N1B4_5677 - putative exported protein, at 113,873 to 114,394
_5677
Pf1N1B4_5678 - sensory box histidine kinase/response regulator, at 114,552 to 116,615
_5678
Pf1N1B4_5679 - DNA polymerase III alpha subunit (EC 2.7.7.7), at 116,795 to 119,872
_5679
Position (kb)
114
115
116
117 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 113.552 kb on - strand, within Pf1N1B4_5676 at 113.848 kb on + strand at 113.849 kb on - strand at 114.601 kb on + strand at 114.619 kb on + strand at 114.619 kb on + strand at 114.619 kb on + strand at 114.653 kb on + strand at 114.653 kb on + strand at 114.653 kb on + strand at 114.653 kb on + strand at 114.654 kb on - strand at 114.654 kb on - strand at 114.654 kb on - strand at 114.654 kb on - strand at 114.986 kb on - strand, within Pf1N1B4_5678 at 114.986 kb on - strand, within Pf1N1B4_5678 at 114.986 kb on - strand, within Pf1N1B4_5678 at 115.186 kb on + strand, within Pf1N1B4_5678 at 115.186 kb on + strand, within Pf1N1B4_5678 at 115.187 kb on - strand, within Pf1N1B4_5678 at 115.236 kb on - strand, within Pf1N1B4_5678 at 115.339 kb on + strand, within Pf1N1B4_5678 at 115.340 kb on - strand, within Pf1N1B4_5678 at 115.382 kb on + strand, within Pf1N1B4_5678 at 115.382 kb on + strand, within Pf1N1B4_5678 at 115.867 kb on + strand at 115.906 kb on + strand, within Pf1N1B4_5678 at 115.906 kb on + strand at 116.260 kb on + strand, within Pf1N1B4_5678 at 116.260 kb on + strand at 116.261 kb on - strand, within Pf1N1B4_5678 at 116.422 kb on + strand at 116.453 kb on - strand at 116.463 kb on - strand at 116.464 kb on + strand at 116.464 kb on + strand at 116.464 kb on + strand at 116.473 kb on + strand at 116.474 kb on - strand at 116.523 kb on + strand at 116.633 kb on + strand at 116.633 kb on + strand at 116.775 kb on + strand at 116.775 kb on + strand at 116.778 kb on - strand at 116.778 kb on - strand at 116.933 kb on + strand at 117.094 kb on + strand at 117.214 kb on + strand, within Pf1N1B4_5679 at 117.615 kb on + strand at 117.615 kb on + strand, within Pf1N1B4_5679 at 117.615 kb on + strand, within Pf1N1B4_5679
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 5mM remove 113,552 - Pf1N1B4_5676 0.58 -0.2 113,848 + -1.1 113,849 - +0.1 114,601 + +0.5 114,619 + -1.2 114,619 + +1.9 114,619 + -0.7 114,653 + -0.5 114,653 + +0.9 114,653 + +2.3 114,653 + -0.8 114,654 - +0.4 114,654 - +0.1 114,654 - +0.0 114,654 - +1.1 114,986 - Pf1N1B4_5678 0.21 -1.4 114,986 - Pf1N1B4_5678 0.21 -3.6 114,986 - Pf1N1B4_5678 0.21 -3.0 115,186 + Pf1N1B4_5678 0.31 -0.8 115,186 + Pf1N1B4_5678 0.31 -0.3 115,187 - Pf1N1B4_5678 0.31 -1.8 115,236 - Pf1N1B4_5678 0.33 +0.0 115,339 + Pf1N1B4_5678 0.38 +0.4 115,340 - Pf1N1B4_5678 0.38 -0.4 115,382 + Pf1N1B4_5678 0.40 -1.1 115,382 + Pf1N1B4_5678 0.40 -1.6 115,867 + +0.7 115,906 + Pf1N1B4_5678 0.66 -0.4 115,906 + +1.3 116,260 + Pf1N1B4_5678 0.83 +0.4 116,260 + -0.9 116,261 - Pf1N1B4_5678 0.83 -1.8 116,422 + -1.4 116,453 - +1.0 116,463 - -1.5 116,464 + +0.6 116,464 + +0.6 116,464 + +0.5 116,473 + +0.6 116,474 - +0.1 116,523 + -0.4 116,633 + +0.5 116,633 + +0.9 116,775 + +0.2 116,775 + +0.3 116,778 - +0.0 116,778 - -1.8 116,933 + -0.3 117,094 + +0.7 117,214 + Pf1N1B4_5679 0.14 +0.8 117,615 + +1.0 117,615 + Pf1N1B4_5679 0.27 -2.6 117,615 + Pf1N1B4_5679 0.27 -0.8
Or see this region's nucleotide sequence