Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4622

Experiment: nitrogen source nitrate 5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4621 and Pf1N1B4_4622 are separated by 194 nucleotidesPf1N1B4_4622 and Pf1N1B4_4623 are separated by 157 nucleotides Pf1N1B4_4621 - L-arabonate dehydratase (EC 4.2.1.25), at 5,096,202 to 5,097,938 _4621 Pf1N1B4_4622 - Nitrate/nitrite transporter, at 5,098,133 to 5,099,455 _4622 Pf1N1B4_4623 - 2-dehydro-3-deoxy-L-arabinonate dehydratase (EC 4.2.1.43) (from data), at 5,099,613 to 5,100,605 _4623 Position (kb) 5098 5099 5100Strain fitness (log2 ratio) -2 -1 0 1 2 3at 5097.214 kb on - strand, within Pf1N1B4_4621at 5098.104 kb on + strandat 5098.104 kb on + strandat 5098.104 kb on + strandat 5098.105 kb on - strandat 5098.105 kb on - strandat 5098.105 kb on - strandat 5098.316 kb on + strandat 5098.448 kb on + strand, within Pf1N1B4_4622at 5098.448 kb on + strand, within Pf1N1B4_4622at 5098.448 kb on + strand, within Pf1N1B4_4622at 5098.448 kb on + strand, within Pf1N1B4_4622at 5098.448 kb on + strand, within Pf1N1B4_4622at 5098.449 kb on - strand, within Pf1N1B4_4622at 5098.449 kb on - strand, within Pf1N1B4_4622at 5098.449 kb on - strand, within Pf1N1B4_4622at 5098.449 kb on - strand, within Pf1N1B4_4622at 5098.505 kb on + strand, within Pf1N1B4_4622at 5098.506 kb on - strand, within Pf1N1B4_4622at 5098.508 kb on + strandat 5098.508 kb on + strand, within Pf1N1B4_4622at 5098.508 kb on + strand, within Pf1N1B4_4622at 5098.508 kb on + strandat 5098.508 kb on + strand, within Pf1N1B4_4622at 5098.509 kb on - strand, within Pf1N1B4_4622at 5098.802 kb on + strand, within Pf1N1B4_4622at 5098.802 kb on + strand, within Pf1N1B4_4622at 5098.898 kb on + strand, within Pf1N1B4_4622at 5098.899 kb on - strand, within Pf1N1B4_4622at 5098.899 kb on - strand, within Pf1N1B4_4622at 5098.982 kb on + strand, within Pf1N1B4_4622at 5099.066 kb on + strand, within Pf1N1B4_4622at 5099.066 kb on + strand, within Pf1N1B4_4622at 5099.067 kb on - strand, within Pf1N1B4_4622at 5099.067 kb on - strand, within Pf1N1B4_4622at 5099.067 kb on - strand, within Pf1N1B4_4622at 5099.067 kb on - strand, within Pf1N1B4_4622at 5099.070 kb on + strand, within Pf1N1B4_4622at 5099.071 kb on - strand, within Pf1N1B4_4622at 5099.300 kb on + strand, within Pf1N1B4_4622at 5099.301 kb on - strand, within Pf1N1B4_4622at 5099.327 kb on + strandat 5099.327 kb on + strandat 5099.327 kb on + strandat 5099.600 kb on - strandat 5099.793 kb on + strandat 5100.030 kb on + strand, within Pf1N1B4_4623at 5100.031 kb on - strand, within Pf1N1B4_4623at 5100.031 kb on - strand, within Pf1N1B4_4623at 5100.130 kb on - strand, within Pf1N1B4_4623at 5100.217 kb on - strand, within Pf1N1B4_4623at 5100.217 kb on - strandat 5100.223 kb on - strand, within Pf1N1B4_4623at 5100.255 kb on + strand, within Pf1N1B4_4623at 5100.411 kb on + strand, within Pf1N1B4_4623at 5100.411 kb on + strand, within Pf1N1B4_4623

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrate 5mM
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5,097,214 - Pf1N1B4_4621 0.58 -1.4
5,098,104 + -0.3
5,098,104 + -1.5
5,098,104 + +0.1
5,098,105 - +0.8
5,098,105 - -0.8
5,098,105 - +0.9
5,098,316 + -1.8
5,098,448 + Pf1N1B4_4622 0.24 -1.5
5,098,448 + Pf1N1B4_4622 0.24 -0.3
5,098,448 + Pf1N1B4_4622 0.24 -0.1
5,098,448 + Pf1N1B4_4622 0.24 +0.1
5,098,448 + Pf1N1B4_4622 0.24 +0.9
5,098,449 - Pf1N1B4_4622 0.24 +1.3
5,098,449 - Pf1N1B4_4622 0.24 +1.2
5,098,449 - Pf1N1B4_4622 0.24 -0.0
5,098,449 - Pf1N1B4_4622 0.24 +0.7
5,098,505 + Pf1N1B4_4622 0.28 +0.5
5,098,506 - Pf1N1B4_4622 0.28 +0.5
5,098,508 + -2.2
5,098,508 + Pf1N1B4_4622 0.28 +1.1
5,098,508 + Pf1N1B4_4622 0.28 +2.1
5,098,508 + +1.5
5,098,508 + Pf1N1B4_4622 0.28 -0.0
5,098,509 - Pf1N1B4_4622 0.28 +1.4
5,098,802 + Pf1N1B4_4622 0.51 +1.1
5,098,802 + Pf1N1B4_4622 0.51 +0.3
5,098,898 + Pf1N1B4_4622 0.58 +0.8
5,098,899 - Pf1N1B4_4622 0.58 +0.1
5,098,899 - Pf1N1B4_4622 0.58 -0.7
5,098,982 + Pf1N1B4_4622 0.64 -0.4
5,099,066 + Pf1N1B4_4622 0.71 -0.8
5,099,066 + Pf1N1B4_4622 0.71 +1.8
5,099,067 - Pf1N1B4_4622 0.71 +0.1
5,099,067 - Pf1N1B4_4622 0.71 +0.8
5,099,067 - Pf1N1B4_4622 0.71 +1.1
5,099,067 - Pf1N1B4_4622 0.71 -0.3
5,099,070 + Pf1N1B4_4622 0.71 -0.4
5,099,071 - Pf1N1B4_4622 0.71 +0.5
5,099,300 + Pf1N1B4_4622 0.88 -0.1
5,099,301 - Pf1N1B4_4622 0.88 -0.1
5,099,327 + +0.9
5,099,327 + -0.4
5,099,327 + +0.3
5,099,600 - +3.2
5,099,793 + +1.0
5,100,030 + Pf1N1B4_4623 0.42 +0.2
5,100,031 - Pf1N1B4_4623 0.42 +1.2
5,100,031 - Pf1N1B4_4623 0.42 +1.1
5,100,130 - Pf1N1B4_4623 0.52 +1.2
5,100,217 - Pf1N1B4_4623 0.61 +0.2
5,100,217 - -1.7
5,100,223 - Pf1N1B4_4623 0.61 -0.0
5,100,255 + Pf1N1B4_4623 0.65 -0.6
5,100,411 + Pf1N1B4_4623 0.80 +1.7
5,100,411 + Pf1N1B4_4623 0.80 +0.5

Or see this region's nucleotide sequence