Experiment: nitrogen source nitrate 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4411 and Pf1N1B4_4412 are separated by 137 nucleotides Pf1N1B4_4412 and Pf1N1B4_4413 are separated by 107 nucleotides
Pf1N1B4_4411 - Transcriptional regulator, LysR family, at 4,873,905 to 4,874,828
_4411
Pf1N1B4_4412 - Tartrate dehydrogenase (EC 1.1.1.93) @ Tartrate decarboxylase (EC 4.1.1.73) @ D-malic enzyme (EC 1.1.1.83), at 4,874,966 to 4,876,021
_4412
Pf1N1B4_4413 - Aerobic C4-dicarboxylate transporter for fumarate, L-malate, D-malate, succunate, at 4,876,129 to 4,877,484
_4413
Position (kb)
4874
4875
4876
4877 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4874.023 kb on + strand at 4874.058 kb on + strand, within Pf1N1B4_4411 at 4874.277 kb on + strand, within Pf1N1B4_4411 at 4874.318 kb on - strand, within Pf1N1B4_4411 at 4874.848 kb on - strand at 4874.848 kb on - strand at 4874.848 kb on - strand at 4874.855 kb on + strand at 4874.887 kb on - strand at 4874.890 kb on + strand at 4874.890 kb on + strand at 4874.891 kb on - strand at 4874.892 kb on + strand at 4874.892 kb on + strand at 4874.893 kb on - strand at 4874.976 kb on - strand at 4875.089 kb on + strand, within Pf1N1B4_4412 at 4875.089 kb on + strand, within Pf1N1B4_4412 at 4875.128 kb on + strand, within Pf1N1B4_4412 at 4875.128 kb on + strand, within Pf1N1B4_4412 at 4875.129 kb on - strand, within Pf1N1B4_4412 at 4875.129 kb on - strand, within Pf1N1B4_4412 at 4875.457 kb on + strand, within Pf1N1B4_4412 at 4875.457 kb on + strand, within Pf1N1B4_4412 at 4875.458 kb on - strand, within Pf1N1B4_4412 at 4875.949 kb on + strand at 4876.042 kb on + strand at 4876.043 kb on - strand at 4876.043 kb on - strand at 4876.045 kb on + strand at 4876.045 kb on + strand at 4876.046 kb on - strand at 4876.110 kb on - strand at 4876.153 kb on + strand at 4876.154 kb on - strand at 4876.157 kb on + strand at 4876.157 kb on + strand at 4876.158 kb on - strand at 4876.158 kb on - strand at 4876.158 kb on - strand at 4876.158 kb on - strand at 4876.158 kb on - strand at 4876.158 kb on - strand at 4876.158 kb on - strand at 4876.158 kb on - strand at 4876.314 kb on + strand, within Pf1N1B4_4413 at 4876.314 kb on + strand, within Pf1N1B4_4413 at 4876.364 kb on - strand, within Pf1N1B4_4413 at 4876.464 kb on + strand, within Pf1N1B4_4413 at 4876.483 kb on + strand, within Pf1N1B4_4413 at 4876.484 kb on - strand, within Pf1N1B4_4413 at 4876.757 kb on - strand, within Pf1N1B4_4413 at 4876.836 kb on - strand, within Pf1N1B4_4413 at 4876.914 kb on - strand, within Pf1N1B4_4413 at 4876.914 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 5mM remove 4,874,023 + -1.0 4,874,058 + Pf1N1B4_4411 0.17 -0.9 4,874,277 + Pf1N1B4_4411 0.40 -1.3 4,874,318 - Pf1N1B4_4411 0.45 -0.1 4,874,848 - +1.3 4,874,848 - -0.4 4,874,848 - -0.2 4,874,855 + -0.1 4,874,887 - +1.2 4,874,890 + +1.1 4,874,890 + +1.3 4,874,891 - -1.6 4,874,892 + +0.0 4,874,892 + +0.8 4,874,893 - +0.3 4,874,976 - +2.3 4,875,089 + Pf1N1B4_4412 0.12 -1.4 4,875,089 + Pf1N1B4_4412 0.12 -1.4 4,875,128 + Pf1N1B4_4412 0.15 -1.1 4,875,128 + Pf1N1B4_4412 0.15 -0.8 4,875,129 - Pf1N1B4_4412 0.15 -0.8 4,875,129 - Pf1N1B4_4412 0.15 -0.3 4,875,457 + Pf1N1B4_4412 0.46 -0.4 4,875,457 + Pf1N1B4_4412 0.46 +0.1 4,875,458 - Pf1N1B4_4412 0.47 -0.1 4,875,949 + +1.1 4,876,042 + -0.7 4,876,043 - +0.2 4,876,043 - +0.1 4,876,045 + +0.4 4,876,045 + +0.4 4,876,046 - -0.5 4,876,110 - +1.0 4,876,153 + -1.0 4,876,154 - +1.1 4,876,157 + -1.6 4,876,157 + +0.4 4,876,158 - +0.7 4,876,158 - +0.5 4,876,158 - -1.4 4,876,158 - -0.2 4,876,158 - -0.1 4,876,158 - +0.7 4,876,158 - +0.2 4,876,158 - -0.2 4,876,314 + Pf1N1B4_4413 0.14 +1.0 4,876,314 + Pf1N1B4_4413 0.14 -1.4 4,876,364 - Pf1N1B4_4413 0.17 -0.7 4,876,464 + Pf1N1B4_4413 0.25 -2.3 4,876,483 + Pf1N1B4_4413 0.26 +0.5 4,876,484 - Pf1N1B4_4413 0.26 -0.3 4,876,757 - Pf1N1B4_4413 0.46 -0.5 4,876,836 - Pf1N1B4_4413 0.52 -1.0 4,876,914 - Pf1N1B4_4413 0.58 -0.1 4,876,914 - -1.4
Or see this region's nucleotide sequence