Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4000

Experiment: nitrogen source nitrate 5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3998 and Pf1N1B4_3999 are separated by 240 nucleotidesPf1N1B4_3999 and Pf1N1B4_4000 overlap by 11 nucleotidesPf1N1B4_4000 and Pf1N1B4_4001 are separated by 15 nucleotides Pf1N1B4_3998 - putative exported protein, at 4,443,602 to 4,447,276 _3998 Pf1N1B4_3999 - Transcriptional regulator, MarR family, at 4,447,517 to 4,447,996 _3999 Pf1N1B4_4000 - Outer membrane component of tripartite multidrug resistance system, at 4,447,986 to 4,449,455 _4000 Pf1N1B4_4001 - Membrane fusion component of tripartite multidrug resistance system, at 4,449,471 to 4,450,673 _4001 Position (kb) 4447 4448 4449 4450Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 4447.259 kb on + strandat 4447.260 kb on - strandat 4447.260 kb on - strandat 4447.274 kb on + strandat 4447.274 kb on + strandat 4447.399 kb on - strandat 4447.414 kb on - strandat 4447.440 kb on + strandat 4447.462 kb on + strandat 4447.482 kb on + strandat 4447.483 kb on - strandat 4447.483 kb on - strandat 4447.483 kb on - strandat 4447.977 kb on + strandat 4447.994 kb on + strandat 4447.994 kb on + strandat 4447.995 kb on - strandat 4448.058 kb on + strandat 4448.059 kb on - strandat 4448.059 kb on - strandat 4448.383 kb on - strand, within Pf1N1B4_4000at 4448.419 kb on - strand, within Pf1N1B4_4000at 4449.103 kb on - strand, within Pf1N1B4_4000at 4449.124 kb on - strand, within Pf1N1B4_4000at 4449.124 kb on - strand, within Pf1N1B4_4000at 4449.124 kb on - strand, within Pf1N1B4_4000at 4449.240 kb on + strand, within Pf1N1B4_4000at 4449.240 kb on + strand, within Pf1N1B4_4000at 4449.241 kb on - strand, within Pf1N1B4_4000at 4449.241 kb on - strand, within Pf1N1B4_4000at 4449.241 kb on - strandat 4449.241 kb on - strand, within Pf1N1B4_4000at 4449.279 kb on - strand, within Pf1N1B4_4000at 4449.282 kb on + strand, within Pf1N1B4_4000at 4449.282 kb on + strand, within Pf1N1B4_4000at 4449.453 kb on + strandat 4449.453 kb on + strandat 4449.453 kb on + strandat 4449.485 kb on + strandat 4449.485 kb on + strandat 4449.485 kb on + strandat 4449.486 kb on - strandat 4449.486 kb on - strandat 4449.526 kb on + strandat 4449.527 kb on - strandat 4449.831 kb on + strand, within Pf1N1B4_4001at 4449.831 kb on + strand, within Pf1N1B4_4001at 4450.329 kb on + strand, within Pf1N1B4_4001at 4450.329 kb on + strand, within Pf1N1B4_4001at 4450.330 kb on - strand, within Pf1N1B4_4001

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrate 5mM
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4,447,259 + +0.4
4,447,260 - +0.3
4,447,260 - -1.7
4,447,274 + +0.5
4,447,274 + +0.2
4,447,399 - -0.2
4,447,414 - -0.4
4,447,440 + +0.1
4,447,462 + -0.3
4,447,482 + -1.9
4,447,483 - -0.6
4,447,483 - -1.0
4,447,483 - -0.4
4,447,977 + -0.4
4,447,994 + -1.1
4,447,994 + -0.3
4,447,995 - +1.0
4,448,058 + +0.8
4,448,059 - +1.0
4,448,059 - +0.8
4,448,383 - Pf1N1B4_4000 0.27 -1.6
4,448,419 - Pf1N1B4_4000 0.29 +0.0
4,449,103 - Pf1N1B4_4000 0.76 +0.6
4,449,124 - Pf1N1B4_4000 0.77 +1.5
4,449,124 - Pf1N1B4_4000 0.77 +0.4
4,449,124 - Pf1N1B4_4000 0.77 -1.1
4,449,240 + Pf1N1B4_4000 0.85 +2.9
4,449,240 + Pf1N1B4_4000 0.85 +1.9
4,449,241 - Pf1N1B4_4000 0.85 +1.2
4,449,241 - Pf1N1B4_4000 0.85 -0.9
4,449,241 - -0.2
4,449,241 - Pf1N1B4_4000 0.85 -0.9
4,449,279 - Pf1N1B4_4000 0.88 +1.5
4,449,282 + Pf1N1B4_4000 0.88 +0.6
4,449,282 + Pf1N1B4_4000 0.88 -1.6
4,449,453 + -1.0
4,449,453 + -1.7
4,449,453 + -1.4
4,449,485 + +0.3
4,449,485 + +0.1
4,449,485 + +0.1
4,449,486 - -0.4
4,449,486 - +1.4
4,449,526 + +0.7
4,449,527 - -0.4
4,449,831 + Pf1N1B4_4001 0.30 -0.7
4,449,831 + Pf1N1B4_4001 0.30 +0.0
4,450,329 + Pf1N1B4_4001 0.71 +0.3
4,450,329 + Pf1N1B4_4001 0.71 +1.1
4,450,330 - Pf1N1B4_4001 0.71 -2.8

Or see this region's nucleotide sequence