Experiment: nitrogen source nitrate 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3572 and Pf1N1B4_3573 are separated by 23 nucleotides Pf1N1B4_3573 and Pf1N1B4_3574 are separated by 13 nucleotides
Pf1N1B4_3572 - Flagellar protein FlgJ [peptidoglycan hydrolase] (EC 3.2.1.-), at 3,975,251 to 3,976,549
_3572
Pf1N1B4_3573 - Flagellar hook-associated protein FlgK, at 3,976,573 to 3,978,633
_3573
Pf1N1B4_3574 - Flagellar hook-associated protein FlgL, at 3,978,647 to 3,980,215
_3574
Position (kb)
3976
3977
3978
3979 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3975.941 kb on + strand, within Pf1N1B4_3572 at 3975.942 kb on - strand, within Pf1N1B4_3572 at 3976.002 kb on - strand, within Pf1N1B4_3572 at 3976.024 kb on - strand at 3976.131 kb on - strand, within Pf1N1B4_3572 at 3976.131 kb on - strand, within Pf1N1B4_3572 at 3976.131 kb on - strand, within Pf1N1B4_3572 at 3976.234 kb on + strand, within Pf1N1B4_3572 at 3976.234 kb on + strand, within Pf1N1B4_3572 at 3976.329 kb on - strand, within Pf1N1B4_3572 at 3976.329 kb on - strand, within Pf1N1B4_3572 at 3976.373 kb on - strand, within Pf1N1B4_3572 at 3976.373 kb on - strand, within Pf1N1B4_3572 at 3976.537 kb on + strand at 3976.537 kb on + strand at 3976.545 kb on + strand at 3976.641 kb on + strand at 3976.642 kb on - strand at 3976.726 kb on + strand at 3976.727 kb on - strand at 3976.727 kb on - strand at 3976.769 kb on - strand at 3976.777 kb on + strand at 3976.858 kb on - strand, within Pf1N1B4_3573 at 3977.080 kb on - strand, within Pf1N1B4_3573 at 3977.242 kb on + strand, within Pf1N1B4_3573 at 3977.659 kb on + strand, within Pf1N1B4_3573 at 3977.660 kb on - strand, within Pf1N1B4_3573 at 3977.660 kb on - strand, within Pf1N1B4_3573 at 3977.690 kb on - strand at 3978.051 kb on - strand, within Pf1N1B4_3573 at 3978.051 kb on - strand, within Pf1N1B4_3573 at 3978.147 kb on + strand, within Pf1N1B4_3573 at 3978.148 kb on - strand, within Pf1N1B4_3573 at 3978.148 kb on - strand, within Pf1N1B4_3573 at 3978.284 kb on + strand, within Pf1N1B4_3573 at 3978.553 kb on + strand at 3978.554 kb on - strand at 3978.554 kb on - strand at 3978.554 kb on - strand at 3978.554 kb on - strand at 3978.563 kb on - strand at 3978.563 kb on - strand at 3978.563 kb on - strand at 3978.566 kb on - strand at 3978.566 kb on - strand at 3978.566 kb on - strand at 3978.566 kb on - strand at 3978.605 kb on + strand at 3978.606 kb on - strand at 3978.671 kb on + strand at 3978.671 kb on + strand at 3978.693 kb on - strand at 3979.046 kb on + strand, within Pf1N1B4_3574 at 3979.046 kb on + strand, within Pf1N1B4_3574 at 3979.106 kb on + strand at 3979.106 kb on + strand at 3979.107 kb on - strand, within Pf1N1B4_3574 at 3979.107 kb on - strand, within Pf1N1B4_3574 at 3979.112 kb on + strand, within Pf1N1B4_3574 at 3979.113 kb on - strand, within Pf1N1B4_3574 at 3979.113 kb on - strand, within Pf1N1B4_3574 at 3979.301 kb on + strand, within Pf1N1B4_3574 at 3979.301 kb on + strand, within Pf1N1B4_3574 at 3979.301 kb on + strand, within Pf1N1B4_3574 at 3979.301 kb on + strand, within Pf1N1B4_3574 at 3979.302 kb on - strand, within Pf1N1B4_3574 at 3979.332 kb on - strand, within Pf1N1B4_3574 at 3979.369 kb on + strand, within Pf1N1B4_3574 at 3979.369 kb on + strand, within Pf1N1B4_3574 at 3979.369 kb on + strand, within Pf1N1B4_3574 at 3979.384 kb on + strand, within Pf1N1B4_3574 at 3979.385 kb on - strand, within Pf1N1B4_3574 at 3979.385 kb on - strand, within Pf1N1B4_3574 at 3979.490 kb on - strand, within Pf1N1B4_3574
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 5mM remove 3,975,941 + Pf1N1B4_3572 0.53 -0.0 3,975,942 - Pf1N1B4_3572 0.53 +0.9 3,976,002 - Pf1N1B4_3572 0.58 -0.4 3,976,024 - +0.6 3,976,131 - Pf1N1B4_3572 0.68 +1.9 3,976,131 - Pf1N1B4_3572 0.68 +1.3 3,976,131 - Pf1N1B4_3572 0.68 +1.3 3,976,234 + Pf1N1B4_3572 0.76 +1.5 3,976,234 + Pf1N1B4_3572 0.76 +0.3 3,976,329 - Pf1N1B4_3572 0.83 +0.1 3,976,329 - Pf1N1B4_3572 0.83 +0.3 3,976,373 - Pf1N1B4_3572 0.86 +1.3 3,976,373 - Pf1N1B4_3572 0.86 -0.7 3,976,537 + -1.5 3,976,537 + -0.0 3,976,545 + +0.7 3,976,641 + +1.4 3,976,642 - -0.3 3,976,726 + -1.3 3,976,727 - -0.5 3,976,727 - +1.2 3,976,769 - -0.0 3,976,777 + +0.2 3,976,858 - Pf1N1B4_3573 0.14 -2.7 3,977,080 - Pf1N1B4_3573 0.25 -0.4 3,977,242 + Pf1N1B4_3573 0.32 -1.0 3,977,659 + Pf1N1B4_3573 0.53 -0.0 3,977,660 - Pf1N1B4_3573 0.53 -0.4 3,977,660 - Pf1N1B4_3573 0.53 -0.5 3,977,690 - -0.7 3,978,051 - Pf1N1B4_3573 0.72 -1.1 3,978,051 - Pf1N1B4_3573 0.72 -2.3 3,978,147 + Pf1N1B4_3573 0.76 +0.4 3,978,148 - Pf1N1B4_3573 0.76 -2.4 3,978,148 - Pf1N1B4_3573 0.76 -0.6 3,978,284 + Pf1N1B4_3573 0.83 -0.0 3,978,553 + +0.7 3,978,554 - +0.2 3,978,554 - -2.4 3,978,554 - +0.5 3,978,554 - +0.3 3,978,563 - +0.8 3,978,563 - -1.3 3,978,563 - -0.5 3,978,566 - -0.6 3,978,566 - +0.2 3,978,566 - -0.6 3,978,566 - -0.3 3,978,605 + +1.1 3,978,606 - +0.5 3,978,671 + +0.1 3,978,671 + -1.7 3,978,693 - +1.1 3,979,046 + Pf1N1B4_3574 0.25 +1.4 3,979,046 + Pf1N1B4_3574 0.25 -1.2 3,979,106 + +1.2 3,979,106 + -0.1 3,979,107 - Pf1N1B4_3574 0.29 +0.9 3,979,107 - Pf1N1B4_3574 0.29 +0.0 3,979,112 + Pf1N1B4_3574 0.30 +0.6 3,979,113 - Pf1N1B4_3574 0.30 -0.3 3,979,113 - Pf1N1B4_3574 0.30 -0.1 3,979,301 + Pf1N1B4_3574 0.42 -0.3 3,979,301 + Pf1N1B4_3574 0.42 -0.2 3,979,301 + Pf1N1B4_3574 0.42 -1.3 3,979,301 + Pf1N1B4_3574 0.42 +1.5 3,979,302 - Pf1N1B4_3574 0.42 +1.0 3,979,332 - Pf1N1B4_3574 0.44 +0.3 3,979,369 + Pf1N1B4_3574 0.46 -0.4 3,979,369 + Pf1N1B4_3574 0.46 +0.6 3,979,369 + Pf1N1B4_3574 0.46 -0.2 3,979,384 + Pf1N1B4_3574 0.47 -1.2 3,979,385 - Pf1N1B4_3574 0.47 +1.8 3,979,385 - Pf1N1B4_3574 0.47 +0.4 3,979,490 - Pf1N1B4_3574 0.54 +0.6
Or see this region's nucleotide sequence