Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3573

Experiment: nitrogen source nitrate 5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3572 and Pf1N1B4_3573 are separated by 23 nucleotidesPf1N1B4_3573 and Pf1N1B4_3574 are separated by 13 nucleotides Pf1N1B4_3572 - Flagellar protein FlgJ [peptidoglycan hydrolase] (EC 3.2.1.-), at 3,975,251 to 3,976,549 _3572 Pf1N1B4_3573 - Flagellar hook-associated protein FlgK, at 3,976,573 to 3,978,633 _3573 Pf1N1B4_3574 - Flagellar hook-associated protein FlgL, at 3,978,647 to 3,980,215 _3574 Position (kb) 3976 3977 3978 3979Strain fitness (log2 ratio) -2 -1 0 1 2at 3975.941 kb on + strand, within Pf1N1B4_3572at 3975.942 kb on - strand, within Pf1N1B4_3572at 3976.002 kb on - strand, within Pf1N1B4_3572at 3976.024 kb on - strandat 3976.131 kb on - strand, within Pf1N1B4_3572at 3976.131 kb on - strand, within Pf1N1B4_3572at 3976.131 kb on - strand, within Pf1N1B4_3572at 3976.234 kb on + strand, within Pf1N1B4_3572at 3976.234 kb on + strand, within Pf1N1B4_3572at 3976.329 kb on - strand, within Pf1N1B4_3572at 3976.329 kb on - strand, within Pf1N1B4_3572at 3976.373 kb on - strand, within Pf1N1B4_3572at 3976.373 kb on - strand, within Pf1N1B4_3572at 3976.537 kb on + strandat 3976.537 kb on + strandat 3976.545 kb on + strandat 3976.641 kb on + strandat 3976.642 kb on - strandat 3976.726 kb on + strandat 3976.727 kb on - strandat 3976.727 kb on - strandat 3976.769 kb on - strandat 3976.777 kb on + strandat 3976.858 kb on - strand, within Pf1N1B4_3573at 3977.080 kb on - strand, within Pf1N1B4_3573at 3977.242 kb on + strand, within Pf1N1B4_3573at 3977.659 kb on + strand, within Pf1N1B4_3573at 3977.660 kb on - strand, within Pf1N1B4_3573at 3977.660 kb on - strand, within Pf1N1B4_3573at 3977.690 kb on - strandat 3978.051 kb on - strand, within Pf1N1B4_3573at 3978.051 kb on - strand, within Pf1N1B4_3573at 3978.147 kb on + strand, within Pf1N1B4_3573at 3978.148 kb on - strand, within Pf1N1B4_3573at 3978.148 kb on - strand, within Pf1N1B4_3573at 3978.284 kb on + strand, within Pf1N1B4_3573at 3978.553 kb on + strandat 3978.554 kb on - strandat 3978.554 kb on - strandat 3978.554 kb on - strandat 3978.554 kb on - strandat 3978.563 kb on - strandat 3978.563 kb on - strandat 3978.563 kb on - strandat 3978.566 kb on - strandat 3978.566 kb on - strandat 3978.566 kb on - strandat 3978.566 kb on - strandat 3978.605 kb on + strandat 3978.606 kb on - strandat 3978.671 kb on + strandat 3978.671 kb on + strandat 3978.693 kb on - strandat 3979.046 kb on + strand, within Pf1N1B4_3574at 3979.046 kb on + strand, within Pf1N1B4_3574at 3979.106 kb on + strandat 3979.106 kb on + strandat 3979.107 kb on - strand, within Pf1N1B4_3574at 3979.107 kb on - strand, within Pf1N1B4_3574at 3979.112 kb on + strand, within Pf1N1B4_3574at 3979.113 kb on - strand, within Pf1N1B4_3574at 3979.113 kb on - strand, within Pf1N1B4_3574at 3979.301 kb on + strand, within Pf1N1B4_3574at 3979.301 kb on + strand, within Pf1N1B4_3574at 3979.301 kb on + strand, within Pf1N1B4_3574at 3979.301 kb on + strand, within Pf1N1B4_3574at 3979.302 kb on - strand, within Pf1N1B4_3574at 3979.332 kb on - strand, within Pf1N1B4_3574at 3979.369 kb on + strand, within Pf1N1B4_3574at 3979.369 kb on + strand, within Pf1N1B4_3574at 3979.369 kb on + strand, within Pf1N1B4_3574at 3979.384 kb on + strand, within Pf1N1B4_3574at 3979.385 kb on - strand, within Pf1N1B4_3574at 3979.385 kb on - strand, within Pf1N1B4_3574at 3979.490 kb on - strand, within Pf1N1B4_3574

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrate 5mM
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3,975,941 + Pf1N1B4_3572 0.53 -0.0
3,975,942 - Pf1N1B4_3572 0.53 +0.9
3,976,002 - Pf1N1B4_3572 0.58 -0.4
3,976,024 - +0.6
3,976,131 - Pf1N1B4_3572 0.68 +1.9
3,976,131 - Pf1N1B4_3572 0.68 +1.3
3,976,131 - Pf1N1B4_3572 0.68 +1.3
3,976,234 + Pf1N1B4_3572 0.76 +1.5
3,976,234 + Pf1N1B4_3572 0.76 +0.3
3,976,329 - Pf1N1B4_3572 0.83 +0.1
3,976,329 - Pf1N1B4_3572 0.83 +0.3
3,976,373 - Pf1N1B4_3572 0.86 +1.3
3,976,373 - Pf1N1B4_3572 0.86 -0.7
3,976,537 + -1.5
3,976,537 + -0.0
3,976,545 + +0.7
3,976,641 + +1.4
3,976,642 - -0.3
3,976,726 + -1.3
3,976,727 - -0.5
3,976,727 - +1.2
3,976,769 - -0.0
3,976,777 + +0.2
3,976,858 - Pf1N1B4_3573 0.14 -2.7
3,977,080 - Pf1N1B4_3573 0.25 -0.4
3,977,242 + Pf1N1B4_3573 0.32 -1.0
3,977,659 + Pf1N1B4_3573 0.53 -0.0
3,977,660 - Pf1N1B4_3573 0.53 -0.4
3,977,660 - Pf1N1B4_3573 0.53 -0.5
3,977,690 - -0.7
3,978,051 - Pf1N1B4_3573 0.72 -1.1
3,978,051 - Pf1N1B4_3573 0.72 -2.3
3,978,147 + Pf1N1B4_3573 0.76 +0.4
3,978,148 - Pf1N1B4_3573 0.76 -2.4
3,978,148 - Pf1N1B4_3573 0.76 -0.6
3,978,284 + Pf1N1B4_3573 0.83 -0.0
3,978,553 + +0.7
3,978,554 - +0.2
3,978,554 - -2.4
3,978,554 - +0.5
3,978,554 - +0.3
3,978,563 - +0.8
3,978,563 - -1.3
3,978,563 - -0.5
3,978,566 - -0.6
3,978,566 - +0.2
3,978,566 - -0.6
3,978,566 - -0.3
3,978,605 + +1.1
3,978,606 - +0.5
3,978,671 + +0.1
3,978,671 + -1.7
3,978,693 - +1.1
3,979,046 + Pf1N1B4_3574 0.25 +1.4
3,979,046 + Pf1N1B4_3574 0.25 -1.2
3,979,106 + +1.2
3,979,106 + -0.1
3,979,107 - Pf1N1B4_3574 0.29 +0.9
3,979,107 - Pf1N1B4_3574 0.29 +0.0
3,979,112 + Pf1N1B4_3574 0.30 +0.6
3,979,113 - Pf1N1B4_3574 0.30 -0.3
3,979,113 - Pf1N1B4_3574 0.30 -0.1
3,979,301 + Pf1N1B4_3574 0.42 -0.3
3,979,301 + Pf1N1B4_3574 0.42 -0.2
3,979,301 + Pf1N1B4_3574 0.42 -1.3
3,979,301 + Pf1N1B4_3574 0.42 +1.5
3,979,302 - Pf1N1B4_3574 0.42 +1.0
3,979,332 - Pf1N1B4_3574 0.44 +0.3
3,979,369 + Pf1N1B4_3574 0.46 -0.4
3,979,369 + Pf1N1B4_3574 0.46 +0.6
3,979,369 + Pf1N1B4_3574 0.46 -0.2
3,979,384 + Pf1N1B4_3574 0.47 -1.2
3,979,385 - Pf1N1B4_3574 0.47 +1.8
3,979,385 - Pf1N1B4_3574 0.47 +0.4
3,979,490 - Pf1N1B4_3574 0.54 +0.6

Or see this region's nucleotide sequence