Experiment: nitrogen source nitrate 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2499 and Pf1N1B4_2500 are separated by 4 nucleotides Pf1N1B4_2500 and Pf1N1B4_2501 overlap by 4 nucleotides Pf1N1B4_2501 and Pf1N1B4_2502 are separated by 127 nucleotides
Pf1N1B4_2499 - Coenzyme PQQ synthesis protein E, at 2,835,034 to 2,836,176
_2499
Pf1N1B4_2500 - Coenzyme PQQ synthesis protein D, at 2,836,181 to 2,836,456
_2500
Pf1N1B4_2501 - Pyrroloquinoline-quinone synthase (EC 1.3.3.11), at 2,836,453 to 2,837,205
_2501
Pf1N1B4_2502 - Coenzyme PQQ synthesis protein B, at 2,837,333 to 2,838,244
_2502
Position (kb)
2836
2837
2838 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2835.455 kb on + strand, within Pf1N1B4_2499 at 2835.456 kb on - strand, within Pf1N1B4_2499 at 2835.458 kb on + strand, within Pf1N1B4_2499 at 2835.459 kb on - strand, within Pf1N1B4_2499 at 2835.459 kb on - strand, within Pf1N1B4_2499 at 2835.519 kb on - strand, within Pf1N1B4_2499 at 2835.558 kb on + strand, within Pf1N1B4_2499 at 2835.790 kb on - strand, within Pf1N1B4_2499 at 2835.904 kb on - strand, within Pf1N1B4_2499 at 2835.905 kb on + strand, within Pf1N1B4_2499 at 2835.906 kb on - strand, within Pf1N1B4_2499 at 2836.085 kb on + strand at 2836.085 kb on + strand at 2836.109 kb on + strand at 2836.110 kb on - strand at 2836.162 kb on + strand at 2836.172 kb on - strand at 2836.172 kb on - strand at 2836.402 kb on - strand, within Pf1N1B4_2500 at 2836.456 kb on + strand at 2836.456 kb on + strand at 2836.456 kb on + strand at 2836.456 kb on + strand at 2836.456 kb on + strand at 2836.456 kb on + strand at 2836.456 kb on + strand at 2836.457 kb on - strand at 2836.457 kb on - strand at 2836.457 kb on - strand at 2836.457 kb on - strand at 2836.470 kb on + strand at 2836.470 kb on + strand at 2836.471 kb on - strand at 2836.523 kb on + strand at 2836.611 kb on + strand, within Pf1N1B4_2501 at 2836.611 kb on + strand, within Pf1N1B4_2501 at 2836.611 kb on + strand, within Pf1N1B4_2501 at 2836.611 kb on + strand, within Pf1N1B4_2501 at 2836.612 kb on - strand, within Pf1N1B4_2501 at 2836.616 kb on + strand, within Pf1N1B4_2501 at 2836.616 kb on + strand, within Pf1N1B4_2501 at 2836.616 kb on + strand, within Pf1N1B4_2501 at 2836.616 kb on + strand, within Pf1N1B4_2501 at 2836.616 kb on + strand, within Pf1N1B4_2501 at 2836.617 kb on - strand, within Pf1N1B4_2501 at 2836.617 kb on - strand, within Pf1N1B4_2501 at 2836.617 kb on - strand, within Pf1N1B4_2501 at 2836.617 kb on - strand, within Pf1N1B4_2501 at 2836.617 kb on - strand, within Pf1N1B4_2501 at 2836.617 kb on - strand, within Pf1N1B4_2501 at 2836.617 kb on - strand, within Pf1N1B4_2501 at 2836.686 kb on - strand, within Pf1N1B4_2501 at 2836.686 kb on - strand, within Pf1N1B4_2501 at 2836.686 kb on - strand, within Pf1N1B4_2501 at 2836.686 kb on - strand, within Pf1N1B4_2501 at 2836.692 kb on - strand, within Pf1N1B4_2501 at 2836.826 kb on + strand, within Pf1N1B4_2501 at 2836.826 kb on + strand, within Pf1N1B4_2501 at 2836.826 kb on + strand, within Pf1N1B4_2501 at 2836.826 kb on + strand, within Pf1N1B4_2501 at 2836.826 kb on + strand, within Pf1N1B4_2501 at 2836.826 kb on + strand, within Pf1N1B4_2501 at 2836.827 kb on - strand, within Pf1N1B4_2501 at 2836.827 kb on - strand, within Pf1N1B4_2501 at 2836.827 kb on - strand, within Pf1N1B4_2501 at 2837.052 kb on - strand, within Pf1N1B4_2501 at 2837.054 kb on + strand, within Pf1N1B4_2501 at 2837.054 kb on + strand, within Pf1N1B4_2501 at 2837.055 kb on - strand, within Pf1N1B4_2501 at 2837.108 kb on + strand, within Pf1N1B4_2501 at 2837.108 kb on + strand, within Pf1N1B4_2501 at 2837.108 kb on + strand, within Pf1N1B4_2501 at 2837.108 kb on + strand, within Pf1N1B4_2501 at 2837.108 kb on + strand, within Pf1N1B4_2501 at 2837.108 kb on + strand, within Pf1N1B4_2501 at 2837.108 kb on + strand, within Pf1N1B4_2501 at 2837.108 kb on + strand, within Pf1N1B4_2501 at 2837.108 kb on + strand, within Pf1N1B4_2501 at 2837.108 kb on + strand, within Pf1N1B4_2501 at 2837.108 kb on + strand, within Pf1N1B4_2501 at 2837.108 kb on + strand, within Pf1N1B4_2501 at 2837.109 kb on - strand, within Pf1N1B4_2501 at 2837.109 kb on - strand, within Pf1N1B4_2501 at 2837.109 kb on - strand, within Pf1N1B4_2501 at 2837.109 kb on - strand, within Pf1N1B4_2501 at 2837.109 kb on - strand, within Pf1N1B4_2501 at 2837.109 kb on - strand, within Pf1N1B4_2501 at 2837.109 kb on - strand, within Pf1N1B4_2501 at 2837.109 kb on - strand, within Pf1N1B4_2501 at 2837.109 kb on - strand, within Pf1N1B4_2501 at 2837.109 kb on - strand, within Pf1N1B4_2501 at 2837.109 kb on - strand, within Pf1N1B4_2501 at 2837.109 kb on - strand, within Pf1N1B4_2501 at 2837.109 kb on - strand, within Pf1N1B4_2501 at 2837.109 kb on - strand, within Pf1N1B4_2501 at 2837.109 kb on - strand, within Pf1N1B4_2501 at 2837.109 kb on - strand, within Pf1N1B4_2501 at 2837.109 kb on - strand, within Pf1N1B4_2501 at 2837.123 kb on + strand, within Pf1N1B4_2501 at 2837.132 kb on + strand at 2837.132 kb on + strand at 2837.132 kb on + strand at 2837.132 kb on + strand at 2837.132 kb on + strand at 2837.132 kb on + strand at 2837.132 kb on + strand at 2837.132 kb on + strand at 2837.132 kb on + strand at 2837.132 kb on + strand at 2837.133 kb on - strand at 2837.133 kb on - strand at 2837.133 kb on - strand at 2837.133 kb on - strand at 2837.133 kb on - strand at 2837.133 kb on - strand at 2837.133 kb on - strand at 2837.133 kb on - strand at 2837.133 kb on - strand at 2837.133 kb on - strand at 2837.141 kb on + strand at 2837.141 kb on + strand at 2837.141 kb on + strand at 2837.141 kb on + strand at 2837.141 kb on + strand at 2837.141 kb on + strand at 2837.142 kb on - strand at 2837.142 kb on - strand at 2837.142 kb on - strand at 2837.142 kb on - strand at 2837.142 kb on - strand at 2837.142 kb on - strand at 2837.142 kb on - strand at 2837.144 kb on + strand at 2837.144 kb on + strand at 2837.144 kb on + strand at 2837.144 kb on + strand at 2837.145 kb on - strand at 2837.145 kb on - strand at 2837.145 kb on - strand at 2837.145 kb on - strand at 2837.145 kb on - strand at 2837.221 kb on - strand at 2837.286 kb on - strand at 2837.294 kb on + strand at 2837.294 kb on + strand at 2837.295 kb on - strand at 2837.295 kb on - strand at 2837.295 kb on - strand at 2837.344 kb on + strand at 2837.344 kb on + strand at 2837.344 kb on + strand at 2837.345 kb on - strand at 2837.345 kb on - strand at 2837.345 kb on - strand at 2837.359 kb on - strand at 2837.658 kb on + strand, within Pf1N1B4_2502 at 2837.658 kb on + strand, within Pf1N1B4_2502 at 2837.658 kb on + strand, within Pf1N1B4_2502 at 2837.658 kb on + strand, within Pf1N1B4_2502 at 2837.658 kb on + strand, within Pf1N1B4_2502 at 2837.658 kb on + strand, within Pf1N1B4_2502 at 2837.658 kb on + strand, within Pf1N1B4_2502 at 2837.659 kb on - strand, within Pf1N1B4_2502 at 2837.659 kb on - strand, within Pf1N1B4_2502 at 2837.659 kb on - strand, within Pf1N1B4_2502 at 2837.659 kb on - strand, within Pf1N1B4_2502 at 2837.659 kb on - strand, within Pf1N1B4_2502 at 2837.659 kb on - strand, within Pf1N1B4_2502 at 2837.659 kb on - strand, within Pf1N1B4_2502 at 2837.659 kb on - strand, within Pf1N1B4_2502 at 2837.659 kb on - strand, within Pf1N1B4_2502 at 2837.668 kb on + strand, within Pf1N1B4_2502 at 2837.828 kb on - strand, within Pf1N1B4_2502
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 5mM remove 2,835,455 + Pf1N1B4_2499 0.37 +0.3 2,835,456 - Pf1N1B4_2499 0.37 -2.2 2,835,458 + Pf1N1B4_2499 0.37 -0.4 2,835,459 - Pf1N1B4_2499 0.37 -0.7 2,835,459 - Pf1N1B4_2499 0.37 +0.6 2,835,519 - Pf1N1B4_2499 0.42 -0.9 2,835,558 + Pf1N1B4_2499 0.46 +0.9 2,835,790 - Pf1N1B4_2499 0.66 -0.1 2,835,904 - Pf1N1B4_2499 0.76 -1.3 2,835,905 + Pf1N1B4_2499 0.76 +0.5 2,835,906 - Pf1N1B4_2499 0.76 -1.3 2,836,085 + +0.0 2,836,085 + -2.1 2,836,109 + -1.2 2,836,110 - -2.1 2,836,162 + -0.9 2,836,172 - -1.2 2,836,172 - -1.2 2,836,402 - Pf1N1B4_2500 0.80 +0.2 2,836,456 + +0.3 2,836,456 + +0.8 2,836,456 + -2.5 2,836,456 + -1.5 2,836,456 + -0.9 2,836,456 + -1.5 2,836,456 + -1.6 2,836,457 - -0.6 2,836,457 - -0.7 2,836,457 - +1.0 2,836,457 - -0.7 2,836,470 + -1.9 2,836,470 + -2.7 2,836,471 - -2.6 2,836,523 + +0.0 2,836,611 + Pf1N1B4_2501 0.21 +1.2 2,836,611 + Pf1N1B4_2501 0.21 -2.5 2,836,611 + Pf1N1B4_2501 0.21 -1.7 2,836,611 + Pf1N1B4_2501 0.21 +0.0 2,836,612 - Pf1N1B4_2501 0.21 -1.5 2,836,616 + Pf1N1B4_2501 0.22 +0.4 2,836,616 + Pf1N1B4_2501 0.22 -0.4 2,836,616 + Pf1N1B4_2501 0.22 -0.9 2,836,616 + Pf1N1B4_2501 0.22 -2.1 2,836,616 + Pf1N1B4_2501 0.22 -0.0 2,836,617 - Pf1N1B4_2501 0.22 +0.8 2,836,617 - Pf1N1B4_2501 0.22 -0.2 2,836,617 - Pf1N1B4_2501 0.22 +1.1 2,836,617 - Pf1N1B4_2501 0.22 +1.3 2,836,617 - Pf1N1B4_2501 0.22 -1.1 2,836,617 - Pf1N1B4_2501 0.22 -1.9 2,836,617 - Pf1N1B4_2501 0.22 +0.6 2,836,686 - Pf1N1B4_2501 0.31 -1.9 2,836,686 - Pf1N1B4_2501 0.31 -0.6 2,836,686 - Pf1N1B4_2501 0.31 +1.6 2,836,686 - Pf1N1B4_2501 0.31 -1.5 2,836,692 - Pf1N1B4_2501 0.32 -2.1 2,836,826 + Pf1N1B4_2501 0.50 -0.0 2,836,826 + Pf1N1B4_2501 0.50 -0.3 2,836,826 + Pf1N1B4_2501 0.50 -0.8 2,836,826 + Pf1N1B4_2501 0.50 +0.4 2,836,826 + Pf1N1B4_2501 0.50 -1.7 2,836,826 + Pf1N1B4_2501 0.50 -0.6 2,836,827 - Pf1N1B4_2501 0.50 -2.4 2,836,827 - Pf1N1B4_2501 0.50 +1.6 2,836,827 - Pf1N1B4_2501 0.50 -0.9 2,837,052 - Pf1N1B4_2501 0.80 -2.1 2,837,054 + Pf1N1B4_2501 0.80 +1.1 2,837,054 + Pf1N1B4_2501 0.80 -1.6 2,837,055 - Pf1N1B4_2501 0.80 +0.8 2,837,108 + Pf1N1B4_2501 0.87 -1.4 2,837,108 + Pf1N1B4_2501 0.87 -2.6 2,837,108 + Pf1N1B4_2501 0.87 -1.0 2,837,108 + Pf1N1B4_2501 0.87 -1.7 2,837,108 + Pf1N1B4_2501 0.87 -2.3 2,837,108 + Pf1N1B4_2501 0.87 -1.3 2,837,108 + Pf1N1B4_2501 0.87 -1.7 2,837,108 + Pf1N1B4_2501 0.87 -1.6 2,837,108 + Pf1N1B4_2501 0.87 -2.8 2,837,108 + Pf1N1B4_2501 0.87 -1.8 2,837,108 + Pf1N1B4_2501 0.87 -1.0 2,837,108 + Pf1N1B4_2501 0.87 -0.2 2,837,109 - Pf1N1B4_2501 0.87 -0.6 2,837,109 - Pf1N1B4_2501 0.87 -2.1 2,837,109 - Pf1N1B4_2501 0.87 +1.1 2,837,109 - Pf1N1B4_2501 0.87 -0.2 2,837,109 - Pf1N1B4_2501 0.87 +0.9 2,837,109 - Pf1N1B4_2501 0.87 -0.2 2,837,109 - Pf1N1B4_2501 0.87 -1.1 2,837,109 - Pf1N1B4_2501 0.87 -0.3 2,837,109 - Pf1N1B4_2501 0.87 -1.7 2,837,109 - Pf1N1B4_2501 0.87 -1.3 2,837,109 - Pf1N1B4_2501 0.87 +0.5 2,837,109 - Pf1N1B4_2501 0.87 -1.3 2,837,109 - Pf1N1B4_2501 0.87 -0.5 2,837,109 - Pf1N1B4_2501 0.87 +0.9 2,837,109 - Pf1N1B4_2501 0.87 -2.9 2,837,109 - Pf1N1B4_2501 0.87 -0.8 2,837,109 - Pf1N1B4_2501 0.87 -3.5 2,837,123 + Pf1N1B4_2501 0.89 +0.1 2,837,132 + -2.2 2,837,132 + -1.8 2,837,132 + +0.3 2,837,132 + -0.6 2,837,132 + +0.4 2,837,132 + -2.1 2,837,132 + -2.0 2,837,132 + -1.4 2,837,132 + -1.1 2,837,132 + +0.2 2,837,133 - -1.3 2,837,133 - -0.2 2,837,133 - -1.6 2,837,133 - -0.8 2,837,133 - -2.5 2,837,133 - -1.5 2,837,133 - -2.2 2,837,133 - -2.5 2,837,133 - +0.4 2,837,133 - +1.7 2,837,141 + -2.6 2,837,141 + -2.3 2,837,141 + -0.8 2,837,141 + -0.7 2,837,141 + -0.2 2,837,141 + -0.8 2,837,142 - -0.8 2,837,142 - +2.5 2,837,142 - +1.0 2,837,142 - +0.1 2,837,142 - +0.1 2,837,142 - -1.3 2,837,142 - -0.3 2,837,144 + +1.4 2,837,144 + -1.8 2,837,144 + +2.1 2,837,144 + -0.6 2,837,145 - +0.8 2,837,145 - -1.5 2,837,145 - +1.1 2,837,145 - -0.0 2,837,145 - +0.0 2,837,221 - +0.8 2,837,286 - -0.2 2,837,294 + +0.2 2,837,294 + -0.2 2,837,295 - -0.3 2,837,295 - +1.0 2,837,295 - +0.5 2,837,344 + -0.2 2,837,344 + -1.8 2,837,344 + +1.1 2,837,345 - -0.2 2,837,345 - -2.1 2,837,345 - -0.5 2,837,359 - -0.6 2,837,658 + Pf1N1B4_2502 0.36 +0.2 2,837,658 + Pf1N1B4_2502 0.36 -0.5 2,837,658 + Pf1N1B4_2502 0.36 +0.7 2,837,658 + Pf1N1B4_2502 0.36 -1.1 2,837,658 + Pf1N1B4_2502 0.36 -2.3 2,837,658 + Pf1N1B4_2502 0.36 +0.1 2,837,658 + Pf1N1B4_2502 0.36 -0.3 2,837,659 - Pf1N1B4_2502 0.36 -0.1 2,837,659 - Pf1N1B4_2502 0.36 -1.9 2,837,659 - Pf1N1B4_2502 0.36 -1.3 2,837,659 - Pf1N1B4_2502 0.36 -2.3 2,837,659 - Pf1N1B4_2502 0.36 -1.5 2,837,659 - Pf1N1B4_2502 0.36 -1.0 2,837,659 - Pf1N1B4_2502 0.36 -0.3 2,837,659 - Pf1N1B4_2502 0.36 -0.8 2,837,659 - Pf1N1B4_2502 0.36 -1.9 2,837,668 + Pf1N1B4_2502 0.37 -1.1 2,837,828 - Pf1N1B4_2502 0.54 -2.0
Or see this region's nucleotide sequence