Experiment: nitrogen source nitrate 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1973 and Pf1N1B4_1974 are separated by 3 nucleotides Pf1N1B4_1974 and Pf1N1B4_1975 are separated by 146 nucleotides Pf1N1B4_1975 and Pf1N1B4_1976 are separated by 122 nucleotides
Pf1N1B4_1973 - FIG00964095: hypothetical protein, at 2,255,312 to 2,257,444
_1973
Pf1N1B4_1974 - L-lysine permease, at 2,257,448 to 2,258,077
_1974
Pf1N1B4_1975 - Transcriptional regulator, AsnC family, at 2,258,224 to 2,258,697
_1975
Pf1N1B4_1976 - Dihydrolipoamide dehydrogenase (EC 1.8.1.4), at 2,258,820 to 2,260,220
_1976
Position (kb)
2257
2258
2259 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2256.480 kb on - strand, within Pf1N1B4_1973 at 2256.548 kb on + strand, within Pf1N1B4_1973 at 2256.549 kb on - strand, within Pf1N1B4_1973 at 2256.549 kb on - strand, within Pf1N1B4_1973 at 2256.549 kb on - strand, within Pf1N1B4_1973 at 2256.549 kb on - strand, within Pf1N1B4_1973 at 2256.549 kb on - strand, within Pf1N1B4_1973 at 2256.573 kb on - strand, within Pf1N1B4_1973 at 2256.590 kb on + strand, within Pf1N1B4_1973 at 2256.590 kb on + strand, within Pf1N1B4_1973 at 2256.591 kb on - strand, within Pf1N1B4_1973 at 2256.591 kb on - strand, within Pf1N1B4_1973 at 2256.591 kb on - strand, within Pf1N1B4_1973 at 2256.596 kb on + strand, within Pf1N1B4_1973 at 2256.597 kb on - strand, within Pf1N1B4_1973 at 2256.650 kb on + strand at 2256.650 kb on + strand, within Pf1N1B4_1973 at 2256.650 kb on + strand, within Pf1N1B4_1973 at 2256.650 kb on + strand, within Pf1N1B4_1973 at 2256.650 kb on + strand, within Pf1N1B4_1973 at 2256.651 kb on - strand, within Pf1N1B4_1973 at 2256.651 kb on - strand, within Pf1N1B4_1973 at 2256.651 kb on - strand, within Pf1N1B4_1973 at 2256.651 kb on - strand, within Pf1N1B4_1973 at 2256.651 kb on - strand, within Pf1N1B4_1973 at 2256.651 kb on - strand, within Pf1N1B4_1973 at 2256.703 kb on + strand, within Pf1N1B4_1973 at 2256.704 kb on - strand, within Pf1N1B4_1973 at 2256.707 kb on + strand, within Pf1N1B4_1973 at 2256.707 kb on + strand, within Pf1N1B4_1973 at 2256.707 kb on + strand, within Pf1N1B4_1973 at 2256.707 kb on + strand, within Pf1N1B4_1973 at 2256.707 kb on + strand, within Pf1N1B4_1973 at 2256.707 kb on + strand, within Pf1N1B4_1973 at 2256.707 kb on + strand, within Pf1N1B4_1973 at 2256.707 kb on + strand, within Pf1N1B4_1973 at 2256.707 kb on + strand, within Pf1N1B4_1973 at 2256.707 kb on + strand, within Pf1N1B4_1973 at 2256.707 kb on + strand, within Pf1N1B4_1973 at 2256.707 kb on + strand, within Pf1N1B4_1973 at 2256.707 kb on + strand at 2256.707 kb on + strand, within Pf1N1B4_1973 at 2256.707 kb on + strand, within Pf1N1B4_1973 at 2256.707 kb on + strand, within Pf1N1B4_1973 at 2256.707 kb on + strand, within Pf1N1B4_1973 at 2256.707 kb on + strand, within Pf1N1B4_1973 at 2256.707 kb on + strand, within Pf1N1B4_1973 at 2256.707 kb on + strand at 2256.707 kb on + strand, within Pf1N1B4_1973 at 2256.708 kb on - strand, within Pf1N1B4_1973 at 2256.708 kb on - strand, within Pf1N1B4_1973 at 2256.708 kb on - strand, within Pf1N1B4_1973 at 2256.708 kb on - strand, within Pf1N1B4_1973 at 2256.708 kb on - strand, within Pf1N1B4_1973 at 2256.708 kb on - strand, within Pf1N1B4_1973 at 2256.708 kb on - strand, within Pf1N1B4_1973 at 2256.708 kb on - strand, within Pf1N1B4_1973 at 2256.708 kb on - strand, within Pf1N1B4_1973 at 2256.708 kb on - strand, within Pf1N1B4_1973 at 2256.708 kb on - strand, within Pf1N1B4_1973 at 2256.708 kb on - strand, within Pf1N1B4_1973 at 2256.708 kb on - strand, within Pf1N1B4_1973 at 2256.708 kb on - strand, within Pf1N1B4_1973 at 2256.708 kb on - strand, within Pf1N1B4_1973 at 2256.708 kb on - strand, within Pf1N1B4_1973 at 2256.777 kb on - strand, within Pf1N1B4_1973 at 2256.795 kb on - strand, within Pf1N1B4_1973 at 2256.795 kb on - strand, within Pf1N1B4_1973 at 2256.795 kb on - strand, within Pf1N1B4_1973 at 2256.833 kb on + strand, within Pf1N1B4_1973 at 2256.834 kb on - strand, within Pf1N1B4_1973 at 2256.894 kb on - strand, within Pf1N1B4_1973 at 2256.894 kb on - strand, within Pf1N1B4_1973 at 2256.896 kb on + strand at 2256.896 kb on + strand, within Pf1N1B4_1973 at 2256.896 kb on + strand, within Pf1N1B4_1973 at 2256.896 kb on + strand, within Pf1N1B4_1973 at 2256.896 kb on + strand, within Pf1N1B4_1973 at 2256.896 kb on + strand, within Pf1N1B4_1973 at 2256.896 kb on + strand, within Pf1N1B4_1973 at 2256.896 kb on + strand, within Pf1N1B4_1973 at 2256.896 kb on + strand, within Pf1N1B4_1973 at 2256.896 kb on + strand, within Pf1N1B4_1973 at 2256.896 kb on + strand, within Pf1N1B4_1973 at 2256.896 kb on + strand, within Pf1N1B4_1973 at 2256.897 kb on - strand, within Pf1N1B4_1973 at 2256.897 kb on - strand, within Pf1N1B4_1973 at 2256.897 kb on - strand, within Pf1N1B4_1973 at 2256.897 kb on - strand, within Pf1N1B4_1973 at 2256.899 kb on + strand, within Pf1N1B4_1973 at 2256.899 kb on + strand, within Pf1N1B4_1973 at 2256.899 kb on + strand, within Pf1N1B4_1973 at 2256.899 kb on + strand, within Pf1N1B4_1973 at 2256.899 kb on + strand, within Pf1N1B4_1973 at 2256.899 kb on + strand, within Pf1N1B4_1973 at 2256.900 kb on - strand, within Pf1N1B4_1973 at 2256.920 kb on + strand, within Pf1N1B4_1973 at 2256.936 kb on + strand, within Pf1N1B4_1973 at 2256.937 kb on - strand, within Pf1N1B4_1973 at 2256.937 kb on - strand, within Pf1N1B4_1973 at 2256.983 kb on + strand, within Pf1N1B4_1973 at 2256.983 kb on + strand, within Pf1N1B4_1973 at 2256.983 kb on + strand, within Pf1N1B4_1973 at 2256.984 kb on - strand, within Pf1N1B4_1973 at 2256.984 kb on - strand, within Pf1N1B4_1973 at 2256.984 kb on - strand, within Pf1N1B4_1973 at 2256.984 kb on - strand, within Pf1N1B4_1973 at 2257.091 kb on + strand, within Pf1N1B4_1973 at 2257.091 kb on + strand, within Pf1N1B4_1973 at 2257.091 kb on + strand, within Pf1N1B4_1973 at 2257.092 kb on - strand, within Pf1N1B4_1973 at 2257.092 kb on - strand, within Pf1N1B4_1973 at 2257.092 kb on - strand, within Pf1N1B4_1973 at 2257.092 kb on - strand, within Pf1N1B4_1973 at 2257.138 kb on + strand, within Pf1N1B4_1973 at 2257.139 kb on - strand, within Pf1N1B4_1973 at 2257.224 kb on - strand, within Pf1N1B4_1973 at 2257.227 kb on + strand, within Pf1N1B4_1973 at 2257.227 kb on + strand, within Pf1N1B4_1973 at 2257.227 kb on + strand, within Pf1N1B4_1973 at 2257.227 kb on + strand, within Pf1N1B4_1973 at 2257.228 kb on - strand, within Pf1N1B4_1973 at 2257.228 kb on - strand, within Pf1N1B4_1973 at 2257.241 kb on + strand at 2257.241 kb on + strand at 2257.241 kb on + strand at 2257.241 kb on + strand at 2257.241 kb on + strand at 2257.241 kb on + strand at 2257.241 kb on + strand at 2257.241 kb on + strand at 2257.241 kb on + strand at 2257.241 kb on + strand at 2257.241 kb on + strand at 2257.241 kb on + strand at 2257.241 kb on + strand at 2257.242 kb on - strand at 2257.303 kb on + strand at 2257.310 kb on + strand at 2257.310 kb on + strand at 2257.310 kb on + strand at 2257.311 kb on - strand at 2257.311 kb on - strand at 2257.311 kb on - strand at 2257.311 kb on - strand at 2257.311 kb on - strand at 2257.355 kb on + strand at 2257.355 kb on + strand at 2257.394 kb on - strand at 2257.410 kb on + strand at 2257.411 kb on - strand at 2257.432 kb on + strand at 2257.432 kb on + strand at 2257.433 kb on - strand at 2257.433 kb on - strand at 2257.578 kb on + strand, within Pf1N1B4_1974 at 2257.941 kb on - strand, within Pf1N1B4_1974 at 2258.034 kb on + strand at 2258.034 kb on + strand at 2258.035 kb on - strand at 2258.035 kb on - strand at 2258.035 kb on - strand at 2258.035 kb on - strand at 2258.117 kb on + strand at 2258.117 kb on + strand at 2258.117 kb on + strand at 2258.117 kb on + strand at 2258.118 kb on - strand at 2258.118 kb on - strand at 2258.118 kb on - strand at 2258.118 kb on - strand at 2258.118 kb on - strand at 2258.179 kb on - strand at 2258.575 kb on + strand, within Pf1N1B4_1975 at 2258.576 kb on - strand, within Pf1N1B4_1975 at 2258.576 kb on - strand, within Pf1N1B4_1975 at 2258.576 kb on - strand, within Pf1N1B4_1975 at 2258.695 kb on + strand at 2258.695 kb on + strand at 2258.695 kb on + strand at 2258.821 kb on + strand at 2258.821 kb on + strand at 2258.821 kb on + strand at 2258.821 kb on + strand at 2258.821 kb on + strand at 2258.821 kb on + strand at 2258.821 kb on + strand at 2258.821 kb on + strand at 2258.822 kb on - strand at 2258.822 kb on - strand at 2258.822 kb on - strand at 2258.822 kb on - strand at 2258.822 kb on - strand at 2258.822 kb on - strand at 2258.822 kb on - strand at 2258.822 kb on - strand at 2259.024 kb on + strand, within Pf1N1B4_1976 at 2259.024 kb on + strand, within Pf1N1B4_1976 at 2259.024 kb on + strand, within Pf1N1B4_1976 at 2259.024 kb on + strand at 2259.025 kb on - strand, within Pf1N1B4_1976 at 2259.025 kb on - strand, within Pf1N1B4_1976 at 2259.025 kb on - strand, within Pf1N1B4_1976 at 2259.025 kb on - strand, within Pf1N1B4_1976 at 2259.025 kb on - strand, within Pf1N1B4_1976 at 2259.025 kb on - strand, within Pf1N1B4_1976
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 5mM remove 2,256,480 - Pf1N1B4_1973 0.55 -2.0 2,256,548 + Pf1N1B4_1973 0.58 -1.7 2,256,549 - Pf1N1B4_1973 0.58 +0.5 2,256,549 - Pf1N1B4_1973 0.58 -0.3 2,256,549 - Pf1N1B4_1973 0.58 -0.8 2,256,549 - Pf1N1B4_1973 0.58 -0.8 2,256,549 - Pf1N1B4_1973 0.58 -0.5 2,256,573 - Pf1N1B4_1973 0.59 -0.4 2,256,590 + Pf1N1B4_1973 0.60 +1.0 2,256,590 + Pf1N1B4_1973 0.60 +1.0 2,256,591 - Pf1N1B4_1973 0.60 +1.6 2,256,591 - Pf1N1B4_1973 0.60 -0.0 2,256,591 - Pf1N1B4_1973 0.60 -1.0 2,256,596 + Pf1N1B4_1973 0.60 -0.8 2,256,597 - Pf1N1B4_1973 0.60 +0.1 2,256,650 + -0.7 2,256,650 + Pf1N1B4_1973 0.63 -0.0 2,256,650 + Pf1N1B4_1973 0.63 +0.3 2,256,650 + Pf1N1B4_1973 0.63 +1.2 2,256,650 + Pf1N1B4_1973 0.63 -0.5 2,256,651 - Pf1N1B4_1973 0.63 -0.7 2,256,651 - Pf1N1B4_1973 0.63 +1.4 2,256,651 - Pf1N1B4_1973 0.63 -0.9 2,256,651 - Pf1N1B4_1973 0.63 -1.5 2,256,651 - Pf1N1B4_1973 0.63 +0.3 2,256,651 - Pf1N1B4_1973 0.63 +0.1 2,256,703 + Pf1N1B4_1973 0.65 -0.3 2,256,704 - Pf1N1B4_1973 0.65 -1.7 2,256,707 + Pf1N1B4_1973 0.65 -0.7 2,256,707 + Pf1N1B4_1973 0.65 +0.5 2,256,707 + Pf1N1B4_1973 0.65 -1.5 2,256,707 + Pf1N1B4_1973 0.65 +0.3 2,256,707 + Pf1N1B4_1973 0.65 +0.7 2,256,707 + Pf1N1B4_1973 0.65 -2.0 2,256,707 + Pf1N1B4_1973 0.65 -0.1 2,256,707 + Pf1N1B4_1973 0.65 +1.1 2,256,707 + Pf1N1B4_1973 0.65 -1.0 2,256,707 + Pf1N1B4_1973 0.65 +0.4 2,256,707 + Pf1N1B4_1973 0.65 -0.5 2,256,707 + Pf1N1B4_1973 0.65 -0.9 2,256,707 + +0.1 2,256,707 + Pf1N1B4_1973 0.65 -0.1 2,256,707 + Pf1N1B4_1973 0.65 +1.1 2,256,707 + Pf1N1B4_1973 0.65 -0.5 2,256,707 + Pf1N1B4_1973 0.65 +1.1 2,256,707 + Pf1N1B4_1973 0.65 +0.5 2,256,707 + Pf1N1B4_1973 0.65 +1.7 2,256,707 + +0.5 2,256,707 + Pf1N1B4_1973 0.65 +1.1 2,256,708 - Pf1N1B4_1973 0.65 +0.9 2,256,708 - Pf1N1B4_1973 0.65 -1.2 2,256,708 - Pf1N1B4_1973 0.65 -0.1 2,256,708 - Pf1N1B4_1973 0.65 -1.7 2,256,708 - Pf1N1B4_1973 0.65 +1.5 2,256,708 - Pf1N1B4_1973 0.65 -2.0 2,256,708 - Pf1N1B4_1973 0.65 -0.3 2,256,708 - Pf1N1B4_1973 0.65 +1.8 2,256,708 - Pf1N1B4_1973 0.65 +0.7 2,256,708 - Pf1N1B4_1973 0.65 -2.0 2,256,708 - Pf1N1B4_1973 0.65 -1.0 2,256,708 - Pf1N1B4_1973 0.65 -0.8 2,256,708 - Pf1N1B4_1973 0.65 +0.9 2,256,708 - Pf1N1B4_1973 0.65 +0.7 2,256,708 - Pf1N1B4_1973 0.65 -0.0 2,256,708 - Pf1N1B4_1973 0.65 -0.2 2,256,777 - Pf1N1B4_1973 0.69 -0.2 2,256,795 - Pf1N1B4_1973 0.70 -0.5 2,256,795 - Pf1N1B4_1973 0.70 -1.6 2,256,795 - Pf1N1B4_1973 0.70 -1.1 2,256,833 + Pf1N1B4_1973 0.71 -1.3 2,256,834 - Pf1N1B4_1973 0.71 +0.3 2,256,894 - Pf1N1B4_1973 0.74 -0.6 2,256,894 - Pf1N1B4_1973 0.74 -2.0 2,256,896 + +2.1 2,256,896 + Pf1N1B4_1973 0.74 -1.5 2,256,896 + Pf1N1B4_1973 0.74 -0.2 2,256,896 + Pf1N1B4_1973 0.74 +0.7 2,256,896 + Pf1N1B4_1973 0.74 -0.5 2,256,896 + Pf1N1B4_1973 0.74 +0.1 2,256,896 + Pf1N1B4_1973 0.74 +1.4 2,256,896 + Pf1N1B4_1973 0.74 +1.5 2,256,896 + Pf1N1B4_1973 0.74 -1.1 2,256,896 + Pf1N1B4_1973 0.74 -0.8 2,256,896 + Pf1N1B4_1973 0.74 +1.8 2,256,896 + Pf1N1B4_1973 0.74 +0.4 2,256,897 - Pf1N1B4_1973 0.74 -1.4 2,256,897 - Pf1N1B4_1973 0.74 +0.0 2,256,897 - Pf1N1B4_1973 0.74 -1.0 2,256,897 - Pf1N1B4_1973 0.74 -0.5 2,256,899 + Pf1N1B4_1973 0.74 -0.3 2,256,899 + Pf1N1B4_1973 0.74 +0.5 2,256,899 + Pf1N1B4_1973 0.74 -1.7 2,256,899 + Pf1N1B4_1973 0.74 -2.3 2,256,899 + Pf1N1B4_1973 0.74 +0.5 2,256,899 + Pf1N1B4_1973 0.74 -1.9 2,256,900 - Pf1N1B4_1973 0.74 -0.9 2,256,920 + Pf1N1B4_1973 0.75 -0.6 2,256,936 + Pf1N1B4_1973 0.76 +1.4 2,256,937 - Pf1N1B4_1973 0.76 +1.4 2,256,937 - Pf1N1B4_1973 0.76 +0.7 2,256,983 + Pf1N1B4_1973 0.78 -0.5 2,256,983 + Pf1N1B4_1973 0.78 +0.3 2,256,983 + Pf1N1B4_1973 0.78 +0.3 2,256,984 - Pf1N1B4_1973 0.78 +0.0 2,256,984 - Pf1N1B4_1973 0.78 +0.1 2,256,984 - Pf1N1B4_1973 0.78 +0.2 2,256,984 - Pf1N1B4_1973 0.78 -0.7 2,257,091 + Pf1N1B4_1973 0.83 +0.3 2,257,091 + Pf1N1B4_1973 0.83 +1.3 2,257,091 + Pf1N1B4_1973 0.83 +0.1 2,257,092 - Pf1N1B4_1973 0.83 -1.0 2,257,092 - Pf1N1B4_1973 0.83 +0.5 2,257,092 - Pf1N1B4_1973 0.83 -0.9 2,257,092 - Pf1N1B4_1973 0.83 +0.4 2,257,138 + Pf1N1B4_1973 0.86 -1.5 2,257,139 - Pf1N1B4_1973 0.86 +0.1 2,257,224 - Pf1N1B4_1973 0.90 +0.2 2,257,227 + Pf1N1B4_1973 0.90 +0.6 2,257,227 + Pf1N1B4_1973 0.90 -2.1 2,257,227 + Pf1N1B4_1973 0.90 +2.5 2,257,227 + Pf1N1B4_1973 0.90 -1.1 2,257,228 - Pf1N1B4_1973 0.90 +1.8 2,257,228 - Pf1N1B4_1973 0.90 -0.8 2,257,241 + +0.5 2,257,241 + -0.2 2,257,241 + +0.5 2,257,241 + -0.4 2,257,241 + -2.2 2,257,241 + +0.0 2,257,241 + +0.9 2,257,241 + +0.3 2,257,241 + -1.0 2,257,241 + +0.2 2,257,241 + +1.3 2,257,241 + +0.6 2,257,241 + +1.4 2,257,242 - -1.2 2,257,303 + -1.1 2,257,310 + +0.0 2,257,310 + +0.1 2,257,310 + -0.3 2,257,311 - +1.4 2,257,311 - -0.1 2,257,311 - -2.1 2,257,311 - +0.7 2,257,311 - +0.5 2,257,355 + -0.0 2,257,355 + +0.7 2,257,394 - +0.3 2,257,410 + +0.0 2,257,411 - +0.8 2,257,432 + -0.4 2,257,432 + -0.7 2,257,433 - +0.5 2,257,433 - +0.4 2,257,578 + Pf1N1B4_1974 0.21 -1.0 2,257,941 - Pf1N1B4_1974 0.78 -1.0 2,258,034 + -2.1 2,258,034 + +1.5 2,258,035 - -1.9 2,258,035 - -0.5 2,258,035 - +1.0 2,258,035 - +0.9 2,258,117 + -0.0 2,258,117 + +0.2 2,258,117 + -0.4 2,258,117 + -0.3 2,258,118 - +0.0 2,258,118 - +0.0 2,258,118 - +1.1 2,258,118 - +1.5 2,258,118 - -0.4 2,258,179 - +0.2 2,258,575 + Pf1N1B4_1975 0.74 +0.3 2,258,576 - Pf1N1B4_1975 0.74 -1.5 2,258,576 - Pf1N1B4_1975 0.74 +0.8 2,258,576 - Pf1N1B4_1975 0.74 -1.0 2,258,695 + -2.3 2,258,695 + -0.1 2,258,695 + -0.5 2,258,821 + -1.0 2,258,821 + +1.7 2,258,821 + +0.5 2,258,821 + +1.5 2,258,821 + -0.6 2,258,821 + +0.5 2,258,821 + +0.5 2,258,821 + +0.6 2,258,822 - -1.3 2,258,822 - -0.5 2,258,822 - +0.7 2,258,822 - +0.5 2,258,822 - +0.5 2,258,822 - -0.6 2,258,822 - -0.3 2,258,822 - -0.5 2,259,024 + Pf1N1B4_1976 0.15 +0.0 2,259,024 + Pf1N1B4_1976 0.15 -0.7 2,259,024 + Pf1N1B4_1976 0.15 +1.2 2,259,024 + -1.3 2,259,025 - Pf1N1B4_1976 0.15 -1.6 2,259,025 - Pf1N1B4_1976 0.15 -1.8 2,259,025 - Pf1N1B4_1976 0.15 +0.5 2,259,025 - Pf1N1B4_1976 0.15 +0.2 2,259,025 - Pf1N1B4_1976 0.15 -0.7 2,259,025 - Pf1N1B4_1976 0.15 +2.3
Or see this region's nucleotide sequence