Experiment: nitrogen source nitrate 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1606 and Pf1N1B4_1607 are separated by 353 nucleotides Pf1N1B4_1607 and Pf1N1B4_1608 are separated by 73 nucleotides Pf1N1B4_1608 and Pf1N1B4_1609 overlap by 4 nucleotides
Pf1N1B4_1606 - Glutamine synthetase type I (EC 6.3.1.2), at 1,851,177 to 1,852,583
_1606
Pf1N1B4_1607 - hypothetical protein, at 1,852,937 to 1,853,185
_1607
Pf1N1B4_1608 - FIG00956533: hypothetical protein, at 1,853,259 to 1,853,726
_1608
Pf1N1B4_1609 - FIG00953806: hypothetical protein, at 1,853,723 to 1,854,322
_1609
Position (kb)
1853
1854 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1852.645 kb on - strand at 1852.645 kb on - strand at 1852.687 kb on + strand at 1852.687 kb on + strand at 1852.688 kb on - strand at 1852.688 kb on - strand at 1852.739 kb on + strand at 1852.740 kb on - strand at 1852.740 kb on - strand at 1852.788 kb on + strand at 1852.789 kb on - strand at 1852.789 kb on - strand at 1852.891 kb on + strand at 1852.901 kb on + strand at 1852.901 kb on + strand at 1852.901 kb on + strand at 1852.902 kb on - strand at 1852.902 kb on - strand at 1852.902 kb on - strand at 1852.902 kb on - strand at 1852.902 kb on - strand at 1852.902 kb on - strand at 1852.902 kb on - strand at 1852.902 kb on - strand at 1852.902 kb on - strand at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.009 kb on - strand, within Pf1N1B4_1607 at 1853.009 kb on - strand, within Pf1N1B4_1607 at 1853.009 kb on - strand, within Pf1N1B4_1607 at 1853.009 kb on - strand, within Pf1N1B4_1607 at 1853.009 kb on - strand, within Pf1N1B4_1607 at 1853.026 kb on - strand, within Pf1N1B4_1607 at 1853.026 kb on - strand, within Pf1N1B4_1607 at 1853.026 kb on - strand at 1853.026 kb on - strand, within Pf1N1B4_1607 at 1853.062 kb on + strand, within Pf1N1B4_1607 at 1853.063 kb on - strand, within Pf1N1B4_1607 at 1853.098 kb on + strand, within Pf1N1B4_1607 at 1853.099 kb on - strand, within Pf1N1B4_1607 at 1853.140 kb on + strand, within Pf1N1B4_1607 at 1853.141 kb on - strand, within Pf1N1B4_1607 at 1853.141 kb on - strand, within Pf1N1B4_1607 at 1853.141 kb on - strand, within Pf1N1B4_1607 at 1853.141 kb on - strand, within Pf1N1B4_1607 at 1853.141 kb on - strand, within Pf1N1B4_1607 at 1853.150 kb on + strand, within Pf1N1B4_1607 at 1853.150 kb on + strand, within Pf1N1B4_1607 at 1853.150 kb on + strand, within Pf1N1B4_1607 at 1853.150 kb on + strand, within Pf1N1B4_1607 at 1853.151 kb on - strand, within Pf1N1B4_1607 at 1853.151 kb on - strand, within Pf1N1B4_1607 at 1853.151 kb on - strand, within Pf1N1B4_1607 at 1853.151 kb on - strand, within Pf1N1B4_1607 at 1853.151 kb on - strand, within Pf1N1B4_1607 at 1853.151 kb on - strand, within Pf1N1B4_1607 at 1853.151 kb on - strand, within Pf1N1B4_1607 at 1853.151 kb on - strand, within Pf1N1B4_1607 at 1853.151 kb on - strand, within Pf1N1B4_1607 at 1853.167 kb on + strand at 1853.183 kb on + strand at 1853.183 kb on + strand at 1853.183 kb on + strand at 1853.184 kb on - strand at 1853.184 kb on - strand at 1853.224 kb on + strand at 1853.224 kb on + strand at 1853.224 kb on + strand at 1853.225 kb on - strand at 1853.225 kb on - strand at 1853.225 kb on - strand at 1853.227 kb on + strand at 1853.227 kb on + strand at 1853.227 kb on + strand at 1853.227 kb on + strand at 1853.227 kb on + strand at 1853.227 kb on + strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.233 kb on + strand at 1853.233 kb on + strand at 1853.233 kb on + strand at 1853.233 kb on + strand at 1853.234 kb on - strand at 1853.234 kb on - strand at 1853.234 kb on - strand at 1853.271 kb on + strand at 1853.271 kb on + strand at 1853.272 kb on - strand at 1853.272 kb on - strand at 1853.277 kb on + strand at 1853.277 kb on + strand at 1853.277 kb on + strand at 1853.277 kb on + strand at 1853.277 kb on + strand at 1853.277 kb on + strand at 1853.277 kb on + strand at 1853.277 kb on + strand at 1853.277 kb on + strand at 1853.277 kb on + strand at 1853.278 kb on - strand at 1853.278 kb on - strand at 1853.278 kb on - strand at 1853.278 kb on - strand at 1853.278 kb on - strand at 1853.278 kb on - strand at 1853.278 kb on - strand at 1853.278 kb on - strand at 1853.278 kb on - strand at 1853.515 kb on + strand, within Pf1N1B4_1608 at 1853.560 kb on + strand, within Pf1N1B4_1608 at 1853.561 kb on - strand, within Pf1N1B4_1608 at 1853.704 kb on + strand at 1853.704 kb on + strand at 1853.704 kb on + strand at 1853.705 kb on - strand at 1853.705 kb on - strand at 1853.705 kb on - strand at 1853.705 kb on - strand at 1853.705 kb on - strand at 1853.705 kb on - strand at 1853.717 kb on + strand at 1853.717 kb on + strand at 1853.718 kb on - strand at 1853.718 kb on - strand at 1853.718 kb on - strand at 1853.718 kb on - strand at 1853.718 kb on - strand at 1853.718 kb on - strand at 1853.782 kb on + strand at 1853.783 kb on - strand, within Pf1N1B4_1609 at 1853.783 kb on - strand, within Pf1N1B4_1609 at 1853.786 kb on + strand, within Pf1N1B4_1609 at 1853.786 kb on + strand, within Pf1N1B4_1609 at 1853.786 kb on + strand, within Pf1N1B4_1609 at 1853.787 kb on - strand, within Pf1N1B4_1609 at 1853.787 kb on - strand, within Pf1N1B4_1609 at 1853.787 kb on - strand, within Pf1N1B4_1609 at 1853.787 kb on - strand, within Pf1N1B4_1609 at 1853.787 kb on - strand, within Pf1N1B4_1609 at 1853.836 kb on + strand, within Pf1N1B4_1609 at 1853.983 kb on + strand at 1854.341 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.481 kb on + strand at 1854.482 kb on - strand at 1854.531 kb on + strand at 1854.532 kb on - strand at 1854.534 kb on - strand at 1854.534 kb on - strand at 1854.544 kb on + strand at 1854.557 kb on - strand at 1854.557 kb on - strand at 1854.589 kb on - strand at 1854.589 kb on - strand at 1854.589 kb on - strand at 1854.589 kb on - strand at 1854.648 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 5mM remove 1,852,645 - -0.5 1,852,645 - -0.0 1,852,687 + +0.9 1,852,687 + +1.4 1,852,688 - -2.0 1,852,688 - -0.1 1,852,739 + -0.5 1,852,740 - -2.5 1,852,740 - -0.8 1,852,788 + +0.3 1,852,789 - +0.8 1,852,789 - -0.8 1,852,891 + -0.0 1,852,901 + +0.7 1,852,901 + +1.5 1,852,901 + +0.3 1,852,902 - +0.1 1,852,902 - +0.2 1,852,902 - +0.4 1,852,902 - -0.1 1,852,902 - -3.0 1,852,902 - -1.0 1,852,902 - +1.1 1,852,902 - +0.4 1,852,902 - +0.4 1,853,006 + Pf1N1B4_1607 0.28 +0.7 1,853,006 + -2.4 1,853,006 + Pf1N1B4_1607 0.28 -0.2 1,853,006 + Pf1N1B4_1607 0.28 +0.5 1,853,006 + Pf1N1B4_1607 0.28 +0.4 1,853,006 + Pf1N1B4_1607 0.28 -1.8 1,853,006 + Pf1N1B4_1607 0.28 +0.1 1,853,006 + Pf1N1B4_1607 0.28 -1.4 1,853,006 + Pf1N1B4_1607 0.28 -0.8 1,853,006 + Pf1N1B4_1607 0.28 -0.3 1,853,006 + Pf1N1B4_1607 0.28 -0.0 1,853,006 + Pf1N1B4_1607 0.28 +2.1 1,853,006 + Pf1N1B4_1607 0.28 -0.8 1,853,006 + Pf1N1B4_1607 0.28 +0.2 1,853,007 - Pf1N1B4_1607 0.28 +1.0 1,853,007 - Pf1N1B4_1607 0.28 -0.0 1,853,007 - Pf1N1B4_1607 0.28 +0.9 1,853,007 - Pf1N1B4_1607 0.28 +0.1 1,853,007 - Pf1N1B4_1607 0.28 +0.0 1,853,007 - Pf1N1B4_1607 0.28 +1.1 1,853,007 - Pf1N1B4_1607 0.28 +0.5 1,853,007 - Pf1N1B4_1607 0.28 -1.8 1,853,007 - Pf1N1B4_1607 0.28 -0.7 1,853,007 - Pf1N1B4_1607 0.28 +0.5 1,853,007 - Pf1N1B4_1607 0.28 -0.6 1,853,007 - Pf1N1B4_1607 0.28 +0.3 1,853,007 - Pf1N1B4_1607 0.28 +1.1 1,853,007 - Pf1N1B4_1607 0.28 +1.0 1,853,007 - Pf1N1B4_1607 0.28 -0.7 1,853,007 - Pf1N1B4_1607 0.28 +0.4 1,853,007 - Pf1N1B4_1607 0.28 -1.0 1,853,007 - Pf1N1B4_1607 0.28 +0.8 1,853,007 - Pf1N1B4_1607 0.28 -1.8 1,853,007 - Pf1N1B4_1607 0.28 +0.1 1,853,007 - Pf1N1B4_1607 0.28 +1.3 1,853,007 - Pf1N1B4_1607 0.28 -0.2 1,853,007 - Pf1N1B4_1607 0.28 -0.6 1,853,007 - Pf1N1B4_1607 0.28 -2.0 1,853,009 - Pf1N1B4_1607 0.29 -0.1 1,853,009 - Pf1N1B4_1607 0.29 +0.1 1,853,009 - Pf1N1B4_1607 0.29 -0.5 1,853,009 - Pf1N1B4_1607 0.29 +0.7 1,853,009 - Pf1N1B4_1607 0.29 -1.7 1,853,026 - Pf1N1B4_1607 0.36 +1.6 1,853,026 - Pf1N1B4_1607 0.36 -2.9 1,853,026 - +0.4 1,853,026 - Pf1N1B4_1607 0.36 -0.4 1,853,062 + Pf1N1B4_1607 0.50 +0.5 1,853,063 - Pf1N1B4_1607 0.51 -0.9 1,853,098 + Pf1N1B4_1607 0.65 +0.7 1,853,099 - Pf1N1B4_1607 0.65 +0.4 1,853,140 + Pf1N1B4_1607 0.82 -0.2 1,853,141 - Pf1N1B4_1607 0.82 +0.2 1,853,141 - Pf1N1B4_1607 0.82 +1.0 1,853,141 - Pf1N1B4_1607 0.82 -1.8 1,853,141 - Pf1N1B4_1607 0.82 +0.4 1,853,141 - Pf1N1B4_1607 0.82 -0.7 1,853,150 + Pf1N1B4_1607 0.86 +0.1 1,853,150 + Pf1N1B4_1607 0.86 -0.1 1,853,150 + Pf1N1B4_1607 0.86 +0.9 1,853,150 + Pf1N1B4_1607 0.86 +0.3 1,853,151 - Pf1N1B4_1607 0.86 -1.0 1,853,151 - Pf1N1B4_1607 0.86 +0.4 1,853,151 - Pf1N1B4_1607 0.86 +1.2 1,853,151 - Pf1N1B4_1607 0.86 +1.1 1,853,151 - Pf1N1B4_1607 0.86 +1.4 1,853,151 - Pf1N1B4_1607 0.86 +1.2 1,853,151 - Pf1N1B4_1607 0.86 -1.0 1,853,151 - Pf1N1B4_1607 0.86 -0.6 1,853,151 - Pf1N1B4_1607 0.86 -0.5 1,853,167 + +0.7 1,853,183 + -0.3 1,853,183 + +1.7 1,853,183 + -0.5 1,853,184 - -0.9 1,853,184 - +0.5 1,853,224 + +1.1 1,853,224 + -0.8 1,853,224 + +1.6 1,853,225 - +0.8 1,853,225 - +0.2 1,853,225 - +0.4 1,853,227 + +0.9 1,853,227 + +1.9 1,853,227 + -0.8 1,853,227 + -0.3 1,853,227 + -0.2 1,853,227 + +1.0 1,853,228 - -0.2 1,853,228 - +0.1 1,853,228 - -2.7 1,853,228 - -1.5 1,853,228 - +0.1 1,853,228 - +1.2 1,853,228 - -3.1 1,853,228 - -1.0 1,853,228 - -0.2 1,853,228 - -1.5 1,853,228 - +0.1 1,853,228 - -0.5 1,853,228 - -0.3 1,853,233 + +0.6 1,853,233 + +2.0 1,853,233 + +0.0 1,853,233 + -0.3 1,853,234 - -2.1 1,853,234 - +0.9 1,853,234 - +0.2 1,853,271 + +0.5 1,853,271 + -0.0 1,853,272 - -0.3 1,853,272 - +1.0 1,853,277 + -0.2 1,853,277 + -2.0 1,853,277 + -0.2 1,853,277 + +2.8 1,853,277 + +0.7 1,853,277 + +1.2 1,853,277 + +0.6 1,853,277 + +0.4 1,853,277 + -0.9 1,853,277 + -0.6 1,853,278 - +0.1 1,853,278 - +0.9 1,853,278 - +1.4 1,853,278 - -0.3 1,853,278 - -0.3 1,853,278 - +0.9 1,853,278 - +1.0 1,853,278 - +1.6 1,853,278 - +0.1 1,853,515 + Pf1N1B4_1608 0.55 -0.2 1,853,560 + Pf1N1B4_1608 0.64 -1.0 1,853,561 - Pf1N1B4_1608 0.65 -2.3 1,853,704 + +1.1 1,853,704 + +0.8 1,853,704 + -3.0 1,853,705 - -0.0 1,853,705 - +0.1 1,853,705 - -0.7 1,853,705 - -0.0 1,853,705 - +2.0 1,853,705 - -0.7 1,853,717 + -1.0 1,853,717 + +0.4 1,853,718 - +1.9 1,853,718 - -0.3 1,853,718 - -0.3 1,853,718 - +0.8 1,853,718 - +0.4 1,853,718 - +1.0 1,853,782 + -1.5 1,853,783 - Pf1N1B4_1609 0.10 +0.5 1,853,783 - Pf1N1B4_1609 0.10 -0.7 1,853,786 + Pf1N1B4_1609 0.10 +0.7 1,853,786 + Pf1N1B4_1609 0.10 +0.3 1,853,786 + Pf1N1B4_1609 0.10 +0.4 1,853,787 - Pf1N1B4_1609 0.11 +0.1 1,853,787 - Pf1N1B4_1609 0.11 -1.1 1,853,787 - Pf1N1B4_1609 0.11 +1.1 1,853,787 - Pf1N1B4_1609 0.11 +0.5 1,853,787 - Pf1N1B4_1609 0.11 +0.2 1,853,836 + Pf1N1B4_1609 0.19 -1.1 1,853,983 + -1.5 1,854,341 - +0.4 1,854,348 - +0.8 1,854,348 - +1.4 1,854,348 - -1.2 1,854,348 - +0.3 1,854,348 - +2.3 1,854,348 - -0.2 1,854,348 - -1.5 1,854,348 - +0.7 1,854,348 - +0.4 1,854,348 - -0.3 1,854,348 - +0.3 1,854,481 + +0.5 1,854,482 - -0.9 1,854,531 + -1.7 1,854,532 - +0.9 1,854,534 - -1.2 1,854,534 - -0.1 1,854,544 + -2.0 1,854,557 - +1.1 1,854,557 - +0.2 1,854,589 - -0.5 1,854,589 - +1.3 1,854,589 - +0.2 1,854,589 - -2.0 1,854,648 - -1.0
Or see this region's nucleotide sequence