Experiment: nitrogen source nitrate 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1250 and Pf1N1B4_1251 are separated by 139 nucleotides Pf1N1B4_1251 and Pf1N1B4_1252 are separated by 81 nucleotides
Pf1N1B4_1250 - Nucleoside ABC transporter, periplasmic nucleoside-binding protein, at 1,428,580 to 1,429,662
_1250
Pf1N1B4_1251 - 2-Oxobutyrate oxidase, putative, at 1,429,802 to 1,430,767
_1251
Pf1N1B4_1252 - Adenosine deaminase (EC 3.5.4.4), at 1,430,849 to 1,431,802
_1252
Position (kb)
1429
1430
1431 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1428.850 kb on + strand, within Pf1N1B4_1250 at 1428.851 kb on - strand, within Pf1N1B4_1250 at 1428.851 kb on - strand, within Pf1N1B4_1250 at 1428.851 kb on - strand, within Pf1N1B4_1250 at 1428.851 kb on - strand, within Pf1N1B4_1250 at 1428.851 kb on - strand, within Pf1N1B4_1250 at 1428.880 kb on + strand, within Pf1N1B4_1250 at 1428.974 kb on - strand, within Pf1N1B4_1250 at 1428.974 kb on - strand, within Pf1N1B4_1250 at 1428.974 kb on - strand, within Pf1N1B4_1250 at 1428.974 kb on - strand, within Pf1N1B4_1250 at 1428.988 kb on + strand, within Pf1N1B4_1250 at 1428.988 kb on + strand, within Pf1N1B4_1250 at 1428.988 kb on + strand, within Pf1N1B4_1250 at 1428.989 kb on - strand, within Pf1N1B4_1250 at 1428.989 kb on - strand, within Pf1N1B4_1250 at 1428.989 kb on - strand, within Pf1N1B4_1250 at 1428.989 kb on - strand, within Pf1N1B4_1250 at 1428.989 kb on - strand, within Pf1N1B4_1250 at 1429.120 kb on + strand, within Pf1N1B4_1250 at 1429.120 kb on + strand at 1429.120 kb on + strand at 1429.121 kb on - strand, within Pf1N1B4_1250 at 1429.121 kb on - strand, within Pf1N1B4_1250 at 1429.121 kb on - strand, within Pf1N1B4_1250 at 1429.121 kb on - strand, within Pf1N1B4_1250 at 1429.121 kb on - strand, within Pf1N1B4_1250 at 1429.121 kb on - strand, within Pf1N1B4_1250 at 1429.121 kb on - strand, within Pf1N1B4_1250 at 1429.282 kb on + strand, within Pf1N1B4_1250 at 1429.282 kb on + strand, within Pf1N1B4_1250 at 1429.282 kb on + strand, within Pf1N1B4_1250 at 1429.283 kb on - strand, within Pf1N1B4_1250 at 1429.366 kb on + strand, within Pf1N1B4_1250 at 1429.366 kb on + strand, within Pf1N1B4_1250 at 1429.366 kb on + strand, within Pf1N1B4_1250 at 1429.367 kb on - strand, within Pf1N1B4_1250 at 1429.367 kb on - strand, within Pf1N1B4_1250 at 1429.367 kb on - strand, within Pf1N1B4_1250 at 1429.367 kb on - strand, within Pf1N1B4_1250 at 1429.418 kb on - strand, within Pf1N1B4_1250 at 1429.482 kb on - strand, within Pf1N1B4_1250 at 1429.539 kb on + strand, within Pf1N1B4_1250 at 1429.539 kb on + strand, within Pf1N1B4_1250 at 1429.624 kb on + strand at 1429.624 kb on + strand at 1429.624 kb on + strand at 1429.624 kb on + strand at 1429.625 kb on - strand at 1429.625 kb on - strand at 1429.625 kb on - strand at 1429.625 kb on - strand at 1429.625 kb on - strand at 1429.625 kb on - strand at 1429.625 kb on - strand at 1429.625 kb on - strand at 1429.625 kb on - strand at 1429.625 kb on - strand at 1429.627 kb on + strand at 1429.627 kb on + strand at 1429.627 kb on + strand at 1429.627 kb on + strand at 1429.627 kb on + strand at 1429.627 kb on + strand at 1429.627 kb on + strand at 1429.627 kb on + strand at 1429.628 kb on - strand at 1429.628 kb on - strand at 1429.628 kb on - strand at 1429.661 kb on - strand at 1429.661 kb on - strand at 1429.838 kb on + strand at 1429.913 kb on + strand, within Pf1N1B4_1251 at 1429.913 kb on + strand, within Pf1N1B4_1251 at 1429.914 kb on - strand at 1429.914 kb on - strand, within Pf1N1B4_1251 at 1429.914 kb on - strand, within Pf1N1B4_1251 at 1429.915 kb on + strand, within Pf1N1B4_1251 at 1429.916 kb on - strand, within Pf1N1B4_1251 at 1430.219 kb on + strand, within Pf1N1B4_1251 at 1430.219 kb on + strand, within Pf1N1B4_1251 at 1430.219 kb on + strand, within Pf1N1B4_1251 at 1430.219 kb on + strand, within Pf1N1B4_1251 at 1430.219 kb on + strand, within Pf1N1B4_1251 at 1430.219 kb on + strand, within Pf1N1B4_1251 at 1430.220 kb on - strand, within Pf1N1B4_1251 at 1430.220 kb on - strand, within Pf1N1B4_1251 at 1430.220 kb on - strand, within Pf1N1B4_1251 at 1430.424 kb on - strand at 1430.685 kb on - strand at 1430.685 kb on - strand at 1430.685 kb on - strand at 1430.735 kb on + strand at 1430.735 kb on + strand at 1430.735 kb on + strand at 1430.735 kb on + strand at 1430.735 kb on + strand at 1430.736 kb on - strand at 1430.736 kb on - strand at 1430.736 kb on - strand at 1430.736 kb on - strand at 1430.736 kb on - strand at 1430.736 kb on - strand at 1430.736 kb on - strand at 1430.736 kb on - strand at 1430.736 kb on - strand at 1430.736 kb on - strand at 1430.766 kb on - strand at 1430.766 kb on - strand at 1430.813 kb on + strand at 1430.813 kb on + strand at 1430.813 kb on + strand at 1430.813 kb on + strand at 1430.814 kb on - strand at 1430.814 kb on - strand at 1430.814 kb on - strand at 1430.814 kb on - strand at 1430.852 kb on + strand at 1430.853 kb on - strand at 1430.853 kb on - strand at 1430.993 kb on + strand, within Pf1N1B4_1252 at 1430.993 kb on + strand, within Pf1N1B4_1252 at 1430.994 kb on - strand, within Pf1N1B4_1252 at 1431.029 kb on + strand, within Pf1N1B4_1252 at 1431.029 kb on + strand, within Pf1N1B4_1252 at 1431.029 kb on + strand, within Pf1N1B4_1252 at 1431.030 kb on - strand, within Pf1N1B4_1252 at 1431.030 kb on - strand, within Pf1N1B4_1252 at 1431.030 kb on - strand at 1431.033 kb on - strand at 1431.033 kb on - strand, within Pf1N1B4_1252 at 1431.071 kb on + strand, within Pf1N1B4_1252 at 1431.089 kb on + strand, within Pf1N1B4_1252 at 1431.455 kb on + strand, within Pf1N1B4_1252 at 1431.456 kb on - strand, within Pf1N1B4_1252 at 1431.684 kb on - strand, within Pf1N1B4_1252 at 1431.695 kb on + strand, within Pf1N1B4_1252 at 1431.696 kb on - strand, within Pf1N1B4_1252 at 1431.696 kb on - strand, within Pf1N1B4_1252 at 1431.723 kb on - strand at 1431.723 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 5mM remove 1,428,850 + Pf1N1B4_1250 0.25 -0.9 1,428,851 - Pf1N1B4_1250 0.25 +1.7 1,428,851 - Pf1N1B4_1250 0.25 -0.4 1,428,851 - Pf1N1B4_1250 0.25 +1.7 1,428,851 - Pf1N1B4_1250 0.25 +1.4 1,428,851 - Pf1N1B4_1250 0.25 +1.7 1,428,880 + Pf1N1B4_1250 0.28 -0.5 1,428,974 - Pf1N1B4_1250 0.36 +1.7 1,428,974 - Pf1N1B4_1250 0.36 -0.5 1,428,974 - Pf1N1B4_1250 0.36 -0.7 1,428,974 - Pf1N1B4_1250 0.36 -0.3 1,428,988 + Pf1N1B4_1250 0.38 +0.7 1,428,988 + Pf1N1B4_1250 0.38 +2.7 1,428,988 + Pf1N1B4_1250 0.38 +0.1 1,428,989 - Pf1N1B4_1250 0.38 -0.5 1,428,989 - Pf1N1B4_1250 0.38 -2.2 1,428,989 - Pf1N1B4_1250 0.38 +0.3 1,428,989 - Pf1N1B4_1250 0.38 -0.1 1,428,989 - Pf1N1B4_1250 0.38 -2.1 1,429,120 + Pf1N1B4_1250 0.50 +1.1 1,429,120 + +1.5 1,429,120 + -1.1 1,429,121 - Pf1N1B4_1250 0.50 +0.5 1,429,121 - Pf1N1B4_1250 0.50 -0.0 1,429,121 - Pf1N1B4_1250 0.50 +0.3 1,429,121 - Pf1N1B4_1250 0.50 -0.3 1,429,121 - Pf1N1B4_1250 0.50 -0.3 1,429,121 - Pf1N1B4_1250 0.50 +0.1 1,429,121 - Pf1N1B4_1250 0.50 -0.2 1,429,282 + Pf1N1B4_1250 0.65 -2.1 1,429,282 + Pf1N1B4_1250 0.65 -1.5 1,429,282 + Pf1N1B4_1250 0.65 +0.0 1,429,283 - Pf1N1B4_1250 0.65 -2.5 1,429,366 + Pf1N1B4_1250 0.73 +0.5 1,429,366 + Pf1N1B4_1250 0.73 -0.6 1,429,366 + Pf1N1B4_1250 0.73 +0.8 1,429,367 - Pf1N1B4_1250 0.73 -1.0 1,429,367 - Pf1N1B4_1250 0.73 -1.4 1,429,367 - Pf1N1B4_1250 0.73 +1.4 1,429,367 - Pf1N1B4_1250 0.73 -1.9 1,429,418 - Pf1N1B4_1250 0.77 -1.6 1,429,482 - Pf1N1B4_1250 0.83 +0.4 1,429,539 + Pf1N1B4_1250 0.89 -1.0 1,429,539 + Pf1N1B4_1250 0.89 +0.3 1,429,624 + +0.2 1,429,624 + +0.0 1,429,624 + +0.7 1,429,624 + -1.1 1,429,625 - -1.2 1,429,625 - +1.1 1,429,625 - -1.9 1,429,625 - -0.4 1,429,625 - -0.4 1,429,625 - -1.2 1,429,625 - +0.3 1,429,625 - +0.1 1,429,625 - +1.1 1,429,625 - -1.7 1,429,627 + +0.1 1,429,627 + +1.2 1,429,627 + -1.7 1,429,627 + -0.9 1,429,627 + +1.7 1,429,627 + -1.0 1,429,627 + -0.5 1,429,627 + -0.3 1,429,628 - -1.0 1,429,628 - +1.3 1,429,628 - +0.6 1,429,661 - -1.0 1,429,661 - +0.3 1,429,838 + -1.3 1,429,913 + Pf1N1B4_1251 0.11 -0.5 1,429,913 + Pf1N1B4_1251 0.11 -1.6 1,429,914 - +0.3 1,429,914 - Pf1N1B4_1251 0.12 -0.8 1,429,914 - Pf1N1B4_1251 0.12 -0.3 1,429,915 + Pf1N1B4_1251 0.12 +0.8 1,429,916 - Pf1N1B4_1251 0.12 -1.4 1,430,219 + Pf1N1B4_1251 0.43 -1.1 1,430,219 + Pf1N1B4_1251 0.43 -1.3 1,430,219 + Pf1N1B4_1251 0.43 -0.1 1,430,219 + Pf1N1B4_1251 0.43 -1.3 1,430,219 + Pf1N1B4_1251 0.43 +0.2 1,430,219 + Pf1N1B4_1251 0.43 -0.9 1,430,220 - Pf1N1B4_1251 0.43 -0.7 1,430,220 - Pf1N1B4_1251 0.43 -0.3 1,430,220 - Pf1N1B4_1251 0.43 -0.7 1,430,424 - +1.1 1,430,685 - -1.5 1,430,685 - +0.5 1,430,685 - -1.4 1,430,735 + +0.8 1,430,735 + +0.4 1,430,735 + +1.4 1,430,735 + +1.2 1,430,735 + +0.9 1,430,736 - +0.5 1,430,736 - +0.2 1,430,736 - +0.3 1,430,736 - +2.1 1,430,736 - +0.5 1,430,736 - -0.5 1,430,736 - -0.5 1,430,736 - +0.1 1,430,736 - -0.2 1,430,736 - -2.2 1,430,766 - +0.2 1,430,766 - -0.2 1,430,813 + -0.1 1,430,813 + -0.7 1,430,813 + -0.5 1,430,813 + +0.9 1,430,814 - -0.7 1,430,814 - -0.8 1,430,814 - -0.9 1,430,814 - -1.7 1,430,852 + +0.4 1,430,853 - -0.4 1,430,853 - -0.7 1,430,993 + Pf1N1B4_1252 0.15 -0.5 1,430,993 + Pf1N1B4_1252 0.15 -0.4 1,430,994 - Pf1N1B4_1252 0.15 -0.7 1,431,029 + Pf1N1B4_1252 0.19 +0.1 1,431,029 + Pf1N1B4_1252 0.19 +0.5 1,431,029 + Pf1N1B4_1252 0.19 +0.1 1,431,030 - Pf1N1B4_1252 0.19 +2.1 1,431,030 - Pf1N1B4_1252 0.19 +0.9 1,431,030 - -1.5 1,431,033 - +1.1 1,431,033 - Pf1N1B4_1252 0.19 -1.0 1,431,071 + Pf1N1B4_1252 0.23 +0.0 1,431,089 + Pf1N1B4_1252 0.25 +0.5 1,431,455 + Pf1N1B4_1252 0.64 +0.1 1,431,456 - Pf1N1B4_1252 0.64 -2.6 1,431,684 - Pf1N1B4_1252 0.88 -1.0 1,431,695 + Pf1N1B4_1252 0.89 -0.2 1,431,696 - Pf1N1B4_1252 0.89 +0.2 1,431,696 - Pf1N1B4_1252 0.89 -0.1 1,431,723 - -0.4 1,431,723 - +0.1
Or see this region's nucleotide sequence