Experiment: BHIS with Lithocholic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4507 and BT4508 are separated by 150 nucleotides BT4508 and BT4509 are separated by 174 nucleotides
BT4507: BT4507 - beta-lactamase precursor (NCBI ptt file), at 5,922,082 to 5,922,963
BT4507
BT4508: BT4508 - hypothetical protein (NCBI ptt file), at 5,923,114 to 5,924,340
BT4508
BT4509: BT4509 - conserved hypothetical protein (NCBI ptt file), at 5,924,515 to 5,925,408
BT4509
Position (kb)
5923
5924
5925 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5922.135 kb on + strand at 5922.181 kb on + strand, within BT4507 at 5922.181 kb on + strand, within BT4507 at 5922.182 kb on - strand, within BT4507 at 5922.183 kb on + strand, within BT4507 at 5922.184 kb on - strand, within BT4507 at 5922.184 kb on - strand, within BT4507 at 5922.189 kb on - strand, within BT4507 at 5922.189 kb on - strand, within BT4507 at 5922.371 kb on + strand, within BT4507 at 5922.372 kb on - strand, within BT4507 at 5922.627 kb on - strand, within BT4507 at 5922.743 kb on + strand, within BT4507 at 5922.779 kb on - strand, within BT4507 at 5922.818 kb on - strand, within BT4507 at 5922.921 kb on + strand at 5922.922 kb on - strand at 5922.990 kb on - strand at 5923.017 kb on + strand at 5923.018 kb on - strand at 5923.030 kb on + strand at 5923.055 kb on - strand at 5923.063 kb on + strand at 5923.064 kb on - strand at 5923.111 kb on + strand at 5923.118 kb on + strand at 5923.201 kb on - strand at 5923.201 kb on - strand at 5923.202 kb on + strand at 5923.252 kb on + strand, within BT4508 at 5923.256 kb on + strand, within BT4508 at 5923.256 kb on + strand, within BT4508 at 5923.257 kb on - strand, within BT4508 at 5923.282 kb on - strand, within BT4508 at 5923.282 kb on - strand, within BT4508 at 5923.363 kb on - strand, within BT4508 at 5923.366 kb on + strand, within BT4508 at 5923.366 kb on + strand, within BT4508 at 5923.461 kb on + strand, within BT4508 at 5923.589 kb on + strand, within BT4508 at 5923.589 kb on + strand, within BT4508 at 5923.589 kb on + strand, within BT4508 at 5923.596 kb on + strand, within BT4508 at 5923.715 kb on + strand, within BT4508 at 5923.728 kb on + strand, within BT4508 at 5923.732 kb on + strand, within BT4508 at 5923.751 kb on + strand, within BT4508 at 5923.783 kb on + strand, within BT4508 at 5923.899 kb on + strand, within BT4508 at 5923.899 kb on + strand, within BT4508 at 5923.918 kb on + strand, within BT4508 at 5923.921 kb on - strand, within BT4508 at 5923.967 kb on + strand, within BT4508 at 5924.069 kb on - strand, within BT4508 at 5924.073 kb on + strand, within BT4508 at 5924.074 kb on - strand, within BT4508 at 5924.166 kb on + strand, within BT4508 at 5924.167 kb on - strand, within BT4508 at 5924.239 kb on - strand at 5924.250 kb on + strand at 5924.251 kb on - strand at 5924.312 kb on - strand at 5924.334 kb on + strand at 5924.365 kb on - strand at 5924.365 kb on - strand at 5924.365 kb on - strand at 5924.365 kb on - strand at 5924.471 kb on - strand at 5924.492 kb on + strand at 5924.492 kb on + strand at 5924.493 kb on - strand at 5924.641 kb on - strand, within BT4509 at 5924.644 kb on + strand, within BT4509 at 5924.645 kb on - strand, within BT4509 at 5924.645 kb on - strand, within BT4509 at 5924.645 kb on - strand, within BT4509 at 5924.666 kb on + strand, within BT4509 at 5924.683 kb on - strand, within BT4509 at 5924.702 kb on - strand, within BT4509 at 5924.773 kb on + strand, within BT4509 at 5924.774 kb on - strand, within BT4509 at 5924.847 kb on + strand, within BT4509 at 5924.866 kb on + strand, within BT4509 at 5924.881 kb on - strand, within BT4509 at 5924.886 kb on - strand, within BT4509 at 5925.026 kb on + strand, within BT4509 at 5925.026 kb on + strand, within BT4509 at 5925.105 kb on + strand, within BT4509 at 5925.110 kb on + strand, within BT4509 at 5925.110 kb on + strand, within BT4509 at 5925.259 kb on + strand, within BT4509 at 5925.260 kb on - strand, within BT4509 at 5925.260 kb on - strand, within BT4509 at 5925.275 kb on - strand, within BT4509 at 5925.330 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Lithocholic 0.4 mg/ml remove 5,922,135 + +1.3 5,922,181 + BT4507 0.11 -1.0 5,922,181 + BT4507 0.11 -1.3 5,922,182 - BT4507 0.11 -1.5 5,922,183 + BT4507 0.11 +1.5 5,922,184 - BT4507 0.12 +0.2 5,922,184 - BT4507 0.12 -2.0 5,922,189 - BT4507 0.12 -0.3 5,922,189 - BT4507 0.12 -2.3 5,922,371 + BT4507 0.33 +0.2 5,922,372 - BT4507 0.33 -3.6 5,922,627 - BT4507 0.62 -1.3 5,922,743 + BT4507 0.75 +0.3 5,922,779 - BT4507 0.79 -0.6 5,922,818 - BT4507 0.83 +1.5 5,922,921 + -2.3 5,922,922 - -1.3 5,922,990 - -0.6 5,923,017 + -0.5 5,923,018 - +0.9 5,923,030 + -1.3 5,923,055 - +1.3 5,923,063 + -1.4 5,923,064 - -1.0 5,923,111 + +0.3 5,923,118 + -1.0 5,923,201 - -2.2 5,923,201 - -1.0 5,923,202 + -1.3 5,923,252 + BT4508 0.11 -0.4 5,923,256 + BT4508 0.12 -1.3 5,923,256 + BT4508 0.12 -2.0 5,923,257 - BT4508 0.12 -0.7 5,923,282 - BT4508 0.14 -1.0 5,923,282 - BT4508 0.14 -0.3 5,923,363 - BT4508 0.20 -0.5 5,923,366 + BT4508 0.21 -0.7 5,923,366 + BT4508 0.21 -1.0 5,923,461 + BT4508 0.28 +0.3 5,923,589 + BT4508 0.39 +0.1 5,923,589 + BT4508 0.39 -1.7 5,923,589 + BT4508 0.39 -0.7 5,923,596 + BT4508 0.39 -2.3 5,923,715 + BT4508 0.49 -1.3 5,923,728 + BT4508 0.50 -0.3 5,923,732 + BT4508 0.50 -2.2 5,923,751 + BT4508 0.52 -2.2 5,923,783 + BT4508 0.55 +1.0 5,923,899 + BT4508 0.64 -1.3 5,923,899 + BT4508 0.64 -0.5 5,923,918 + BT4508 0.66 -0.7 5,923,921 - BT4508 0.66 -0.3 5,923,967 + BT4508 0.70 -1.2 5,924,069 - BT4508 0.78 +1.7 5,924,073 + BT4508 0.78 +0.2 5,924,074 - BT4508 0.78 +1.6 5,924,166 + BT4508 0.86 -0.7 5,924,167 - BT4508 0.86 -1.3 5,924,239 - -0.6 5,924,250 + -0.1 5,924,251 - -0.4 5,924,312 - -0.3 5,924,334 + -0.5 5,924,365 - -0.7 5,924,365 - -2.2 5,924,365 - +1.0 5,924,365 - -1.5 5,924,471 - +0.2 5,924,492 + -2.3 5,924,492 + -3.0 5,924,493 - -0.5 5,924,641 - BT4509 0.14 -0.7 5,924,644 + BT4509 0.14 -1.7 5,924,645 - BT4509 0.15 -1.0 5,924,645 - BT4509 0.15 -0.8 5,924,645 - BT4509 0.15 -2.4 5,924,666 + BT4509 0.17 -0.1 5,924,683 - BT4509 0.19 +0.3 5,924,702 - BT4509 0.21 -1.3 5,924,773 + BT4509 0.29 -2.6 5,924,774 - BT4509 0.29 +0.3 5,924,847 + BT4509 0.37 -3.3 5,924,866 + BT4509 0.39 -1.0 5,924,881 - BT4509 0.41 -1.5 5,924,886 - BT4509 0.41 -0.3 5,925,026 + BT4509 0.57 -2.6 5,925,026 + BT4509 0.57 +0.3 5,925,105 + BT4509 0.66 -2.1 5,925,110 + BT4509 0.67 -0.7 5,925,110 + BT4509 0.67 -0.3 5,925,259 + BT4509 0.83 -3.4 5,925,260 - BT4509 0.83 -2.0 5,925,260 - BT4509 0.83 -1.9 5,925,275 - BT4509 0.85 -3.6 5,925,330 - +0.8
Or see this region's nucleotide sequence