Experiment: BHIS with Lithocholic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4445 and BT4446 are separated by 73 nucleotides BT4446 and BT4447 overlap by 1 nucleotides BT4447 and BT4448 are separated by 187 nucleotides
BT4445: BT4445 - conserved hypothetical protein (NCBI ptt file), at 5,855,714 to 5,857,429
BT4445
BT4446: BT4446 - sugar phosphate isomerase/epimerase (NCBI ptt file), at 5,857,503 to 5,858,390
BT4446
BT4447: BT4447 - conserved hypothetical protein (NCBI ptt file), at 5,858,390 to 5,859,112
BT4447
BT4448: BT4448 - putative dehydrogenase (NCBI ptt file), at 5,859,300 to 5,860,775
BT4448
Position (kb)
5857
5858
5859 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5856.596 kb on - strand, within BT4445 at 5856.602 kb on - strand, within BT4445 at 5856.610 kb on + strand, within BT4445 at 5856.678 kb on + strand, within BT4445 at 5856.682 kb on + strand, within BT4445 at 5856.692 kb on - strand, within BT4445 at 5856.692 kb on - strand, within BT4445 at 5856.754 kb on + strand, within BT4445 at 5856.755 kb on - strand, within BT4445 at 5856.769 kb on - strand, within BT4445 at 5856.769 kb on - strand, within BT4445 at 5856.773 kb on + strand, within BT4445 at 5856.774 kb on - strand, within BT4445 at 5856.774 kb on - strand, within BT4445 at 5856.871 kb on + strand, within BT4445 at 5856.877 kb on + strand, within BT4445 at 5856.882 kb on - strand, within BT4445 at 5856.901 kb on + strand, within BT4445 at 5856.923 kb on - strand, within BT4445 at 5856.932 kb on + strand, within BT4445 at 5856.943 kb on - strand, within BT4445 at 5857.144 kb on + strand, within BT4445 at 5857.144 kb on + strand, within BT4445 at 5857.145 kb on - strand, within BT4445 at 5857.221 kb on - strand, within BT4445 at 5857.223 kb on - strand, within BT4445 at 5857.236 kb on + strand, within BT4445 at 5857.251 kb on - strand, within BT4445 at 5857.253 kb on + strand, within BT4445 at 5857.281 kb on + strand at 5857.357 kb on + strand at 5857.361 kb on + strand at 5857.385 kb on + strand at 5857.385 kb on + strand at 5857.385 kb on + strand at 5857.385 kb on + strand at 5857.386 kb on - strand at 5857.386 kb on - strand at 5857.387 kb on + strand at 5857.387 kb on + strand at 5857.424 kb on + strand at 5857.425 kb on - strand at 5857.428 kb on - strand at 5857.463 kb on + strand at 5857.498 kb on + strand at 5857.499 kb on - strand at 5857.509 kb on - strand at 5857.509 kb on - strand at 5857.513 kb on + strand at 5857.514 kb on - strand at 5857.547 kb on - strand at 5857.576 kb on - strand at 5857.588 kb on + strand at 5857.597 kb on - strand, within BT4446 at 5857.610 kb on + strand, within BT4446 at 5857.610 kb on + strand, within BT4446 at 5857.611 kb on - strand, within BT4446 at 5857.611 kb on - strand, within BT4446 at 5857.618 kb on - strand, within BT4446 at 5857.647 kb on - strand, within BT4446 at 5857.647 kb on - strand, within BT4446 at 5857.665 kb on - strand, within BT4446 at 5857.665 kb on - strand, within BT4446 at 5857.670 kb on + strand, within BT4446 at 5857.774 kb on - strand, within BT4446 at 5857.774 kb on - strand, within BT4446 at 5857.796 kb on - strand, within BT4446 at 5857.820 kb on + strand, within BT4446 at 5857.822 kb on + strand, within BT4446 at 5857.822 kb on + strand, within BT4446 at 5857.823 kb on - strand, within BT4446 at 5857.875 kb on - strand, within BT4446 at 5857.986 kb on + strand, within BT4446 at 5857.994 kb on - strand, within BT4446 at 5858.012 kb on + strand, within BT4446 at 5858.112 kb on + strand, within BT4446 at 5858.113 kb on - strand, within BT4446 at 5858.145 kb on - strand, within BT4446 at 5858.185 kb on + strand, within BT4446 at 5858.210 kb on - strand, within BT4446 at 5858.239 kb on - strand, within BT4446 at 5858.263 kb on - strand, within BT4446 at 5858.290 kb on - strand, within BT4446 at 5858.361 kb on + strand at 5858.361 kb on + strand at 5858.366 kb on - strand at 5858.391 kb on + strand at 5858.427 kb on - strand at 5858.621 kb on - strand, within BT4447 at 5858.825 kb on - strand, within BT4447 at 5858.984 kb on - strand, within BT4447 at 5858.984 kb on - strand, within BT4447 at 5859.089 kb on + strand at 5859.262 kb on + strand at 5859.311 kb on + strand at 5859.337 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Lithocholic 0.4 mg/ml remove 5,856,596 - BT4445 0.51 -1.4 5,856,602 - BT4445 0.52 -2.2 5,856,610 + BT4445 0.52 -0.6 5,856,678 + BT4445 0.56 -1.2 5,856,682 + BT4445 0.56 -0.2 5,856,692 - BT4445 0.57 +0.3 5,856,692 - BT4445 0.57 +0.7 5,856,754 + BT4445 0.61 -1.1 5,856,755 - BT4445 0.61 -1.3 5,856,769 - BT4445 0.61 -1.3 5,856,769 - BT4445 0.61 +0.3 5,856,773 + BT4445 0.62 -0.7 5,856,774 - BT4445 0.62 -2.4 5,856,774 - BT4445 0.62 -0.7 5,856,871 + BT4445 0.67 +0.3 5,856,877 + BT4445 0.68 +0.7 5,856,882 - BT4445 0.68 +2.7 5,856,901 + BT4445 0.69 -1.7 5,856,923 - BT4445 0.70 +1.3 5,856,932 + BT4445 0.71 -2.4 5,856,943 - BT4445 0.72 +0.3 5,857,144 + BT4445 0.83 -1.5 5,857,144 + BT4445 0.83 -0.7 5,857,145 - BT4445 0.83 -0.6 5,857,221 - BT4445 0.88 -0.1 5,857,223 - BT4445 0.88 -0.1 5,857,236 + BT4445 0.89 -3.5 5,857,251 - BT4445 0.90 -0.7 5,857,253 + BT4445 0.90 -0.7 5,857,281 + -0.1 5,857,357 + -1.7 5,857,361 + -0.3 5,857,385 + +0.1 5,857,385 + +0.5 5,857,385 + -0.1 5,857,385 + -2.3 5,857,386 - +0.3 5,857,386 - +2.1 5,857,387 + +0.6 5,857,387 + -1.9 5,857,424 + -2.4 5,857,425 - -0.8 5,857,428 - +0.3 5,857,463 + -1.9 5,857,498 + -1.5 5,857,499 - -1.9 5,857,509 - +0.7 5,857,509 - -1.7 5,857,513 + -0.6 5,857,514 - -0.5 5,857,547 - -0.1 5,857,576 - +0.6 5,857,588 + +0.0 5,857,597 - BT4446 0.11 +1.3 5,857,610 + BT4446 0.12 +0.0 5,857,610 + BT4446 0.12 -1.1 5,857,611 - BT4446 0.12 -0.6 5,857,611 - BT4446 0.12 -2.3 5,857,618 - BT4446 0.13 -0.8 5,857,647 - BT4446 0.16 -1.3 5,857,647 - BT4446 0.16 -1.5 5,857,665 - BT4446 0.18 +0.2 5,857,665 - BT4446 0.18 -0.6 5,857,670 + BT4446 0.19 +1.3 5,857,774 - BT4446 0.31 -1.6 5,857,774 - BT4446 0.31 -0.7 5,857,796 - BT4446 0.33 -2.7 5,857,820 + BT4446 0.36 +0.7 5,857,822 + BT4446 0.36 -1.2 5,857,822 + BT4446 0.36 +0.3 5,857,823 - BT4446 0.36 -0.0 5,857,875 - BT4446 0.42 -0.7 5,857,986 + BT4446 0.54 -0.9 5,857,994 - BT4446 0.55 -1.7 5,858,012 + BT4446 0.57 -1.7 5,858,112 + BT4446 0.69 -1.3 5,858,113 - BT4446 0.69 -1.2 5,858,145 - BT4446 0.72 -0.3 5,858,185 + BT4446 0.77 +2.3 5,858,210 - BT4446 0.80 +0.3 5,858,239 - BT4446 0.83 -0.7 5,858,263 - BT4446 0.86 -1.7 5,858,290 - BT4446 0.89 -1.1 5,858,361 + +0.1 5,858,361 + -1.5 5,858,366 - +1.3 5,858,391 + -0.2 5,858,427 - -1.8 5,858,621 - BT4447 0.32 -0.5 5,858,825 - BT4447 0.60 -1.9 5,858,984 - BT4447 0.82 -2.1 5,858,984 - BT4447 0.82 +1.9 5,859,089 + -1.1 5,859,262 + -0.9 5,859,311 + -1.0 5,859,337 + -0.3
Or see this region's nucleotide sequence